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Information on EC 7.3.2.4 - ABC-type nitrate transporter
for references in articles please use BRENDA:EC7.3.2.4
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EC Tree 7 Translocases
7.3 Catalysing the translocation of inorganic anions and their chelates
7.3.2 Linked to the hydrolysis of a nucleoside triphosphate
7.3.2.4 ABC-type nitrate transporter
IUBMB Comments
An ATP-binding cassette (ABC) type transporter, characterized by the presence of two similar ATP-binding domains/proteins and two integral membrane domains/proteins. The enzyme, found in bacteria, interacts with an extracytoplasmic substrate binding protein and mediates the import of nitrate, nitrite, and cyanate.
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
show+
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=
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nitrate-[nitrate-binding protein][side 1]
=
+
+
+
[nitrate-binding protein][side 1]
Synonyms
nitrate transporter, nrt1.1, nrt2.1, atnrt2.1, nark2, nrt1.5, nrt2.5, nrt2.4, nitrate/nitrite transporter, nrt1.8, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
ABC-type nitrate/nitrite transporter
-
-
-
-
ABC-type nitrate/nitrite-bispecific transporter
ABC-type NRT
AtNRT2.1
ATP-binding cassette-type nitrate/nitrite-bispecific transporter
CHL1
CynABD transporter
EC 3.6.3.26
-
-
formerly
-
NarK2
NAX1
nitrate excretion transporter, belongs to the NRT1/PTR transporter family
nitrate transporter
nitrate transporter CHL1
nitrate transporter chlorate resistant 1
nitrate-transporting ATPase
-
-
-
-
nitrate/nitrite transporter
NO3- transporter
NPF7.3
NRT1
NRT1.1
NRT1.3
NRT1.5
NRT2
NRT2.1
NRT2.4
NrtP
-
much higher affinity for nitrate than for nitrite, no post-translational regulation
NrtS
type I H+/nitrate symporter
additional information
-
NRT1.8 is a member of the nitrate transporter, NRT1, family
ATP-binding cassette-type nitrate/nitrite-bispecific transporter
-
-
-
nitrate transporter
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ATP + H2O + nitrate-[nitrate-binding protein][side 1] = ADP + phosphate + nitrate[side 2] + [nitrate-binding protein][side 1]
ATP + H2O + nitrate-[nitrate-binding protein][side 1] = ADP + phosphate + nitrate[side 2] + [nitrate-binding protein][side 1]
-
-
-
-
ATP + H2O + nitrate-[nitrate-binding protein][side 1] = ADP + phosphate + nitrate[side 2] + [nitrate-binding protein][side 1]
phosphorylated His-tagged NrtA1 specifically binds both nitrite and nitrate with high affinity and appears to be saturated at anion concentrations higher than 0.04 mM at pH 8.0 and 45°C
-
ATP + H2O + nitrate-[nitrate-binding protein][side 1] = ADP + phosphate + nitrate[side 2] + [nitrate-binding protein][side 1]
efflux transporter coupled to the ATP-dependent H+ pumping activity, transports intracellular nitrate through the membrane out of the cell
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of phosphoric ester
-
-
-
-
transmembrane transport
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
ATP phosphohydrolase (ABC-type, nitrate-importing)
An ATP-binding cassette (ABC) type transporter, characterized by the presence of two similar ATP-binding domains/proteins and two integral membrane domains/proteins. The enzyme, found in bacteria, interacts with an extracytoplasmic substrate binding protein and mediates the import of nitrate, nitrite, and cyanate.
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CAS REGISTRY NUMBER
COMMENTARY
9000-83-3
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + H2O + abscisic acid/out
ADP + phosphate + abscisic acid/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + nitrate-[nitrate-binding protein][side 1]
ADP + phosphate + nitrate[side 2] + [nitrate-binding protein][side 1]
ATP + H2O + nitrate-[nitrate-binding protein][side 1]
ADP + phosphate + nitrate[side 2] + [nitrate-binding protein][side 1]
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + nitrate-[nitrate-binding protein][side 1]
ADP + phosphate + nitrate[side 2] + [nitrate-binding protein][side 1]
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + nitrate-[nitrate-binding protein][side 1]
ADP + phosphate + nitrate[side 2] + [nitrate-binding protein][side 1]
Substrates: -
Products: -
Products: -
?
ATP + H2O + nitrate-[nitrate-binding protein][side 1]
ADP + phosphate + nitrate[side 2] + [nitrate-binding protein][side 1]
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + nitrate-[nitrate-binding protein][side 1]
ADP + phosphate + nitrate[side 2] + [nitrate-binding protein][side 1]
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + nitrate-[nitrate-binding protein][side 1]
ADP + phosphate + nitrate[side 2] + [nitrate-binding protein][side 1]
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + nitrate/out
ADP + phosphate + nitrate/in
-
Substrates: NRT1.5 is a low-affinity, pH-dependent bidirectional nitrate transporter, that participates in root xylem loading of nitrate
Products: -
Products: -
?
ATP + H2O + nitrate/out
ADP + phosphate + nitrate/in
-
Substrates: NRT1.5 is a low-affinity, pH-dependent bidirectional nitrate transporter, overview
Products: -
Products: -
?
ATP + H2O + nitrate/out
ADP + phosphate + nitrate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + nitrate/out
ADP + phosphate + nitrate/in
-
Substrates: NRT1.8 can transport nitrate and regulates nitrate removal from xylem. Nitrate distribution under Cd2+ stress is altered by NRT1.8
Products: -
Products: -
?
ATP + H2O + nitrate/out
ADP + phosphate + nitrate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + nitrate/out
ADP + phosphate + nitrate/in
Substrates: -
Products: -
Products: -
?
ATP + H2O + nitrate/out
ADP + phosphate + nitrate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + nitrate/out
ADP + phosphate + nitrate/in
-
Substrates: NRT1.5 is a low-affinity, pH-dependent bidirectional nitrate transporter, that participates in root xylem loading of nitrate
Products: -
Products: -
?
ATP + H2O + nitrate/out
ADP + phosphate + nitrate/in
-
Substrates: NRT1.5 is a low-affinity, pH-dependent bidirectional nitrate transporter, overview
Products: -
Products: -
?
ATP + H2O + nitrate/out
ADP + phosphate + nitrate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + nitrate/out
ADP + phosphate + nitrate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + nitrate/out
ADP + phosphate + nitrate/in
Substrates: -
Products: -
Products: -
?
ATP + H2O + nitrate/out
ADP + phosphate + nitrate/in
Substrates: -
Products: -
Products: -
?
ATP + H2O + nitrate/out
ADP + phosphate + nitrate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + nitrate/out
ADP + phosphate + nitrate/in
Substrates: -
Products: -
Products: -
?
ATP + H2O + nitrate/out
ADP + phosphate + nitrate/in
Substrates: -
Products: -
Products: -
?
ATP + H2O + nitrate/out
ADP + phosphate + nitrate/in
Substrates: -
Products: -
Products: -
?
ATP + H2O + nitrate/out
ADP + phosphate + nitrate/in
Substrates: -
Products: -
Products: -
?
ATP + H2O + nitrate/out
ADP + phosphate + nitrate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + nitrate/out
ADP + phosphate + nitrate/in
-
Substrates: the enzyme transports both nitrate and nitrite with high affinity
Products: -
Products: -
?
ATP + H2O + nitrate/out
ADP + phosphate + nitrate/in
-
Substrates: the enzyme transports both nitrate and nitrite with high affinity
Products: -
Products: -
?
ATP + H2O + nitrate/out
ADP + phosphate + nitrate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + nitrate/out
ADP + phosphate + nitrate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + nitrite/out
ADP + phosphate + nitrite/in
Substrates: -
Products: -
Products: -
?
ATP + H2O + nitrite/out
ADP + phosphate + nitrite/in
-
Substrates: the enzyme transports both nitrate and nitrite with high affinity
Products: -
Products: -
?
ATP + H2O + nitrite/out
ADP + phosphate + nitrite/in
-
Substrates: the enzyme transports both nitrate and nitrite with high affinity
Products: -
Products: -
?
ATP + H2O + NO2-/out
ADP + phosphate + NO2-/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + NO2-/out
ADP + phosphate + NO2-/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + NO2-/out
ADP + phosphate + NO2-/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + NO2-/out
ADP + phosphate + NO2-/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + NO2-/out
ADP + phosphate + NO2-/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + NO2-/out
ADP + phosphate + NO2-/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + NO3-/out
ADP + phosphate + NO3-/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + NO3-/out
ADP + phosphate + NO3-/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + NO3-/out
ADP + phosphate + NO3-/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + NO3-/out
ADP + phosphate + NO3-/in
-
Substrates: inactivation of the enzyme at low temperatures limits growth at low temperatures
Products: -
Products: -
?
ATP + H2O + NO3-/out
ADP + phosphate + NO3-/in
-
Substrates: rate-limiting step of nitrate-assimilation, nitrate transport activity is strictly dependent on active CO2-fixation. This positive C-control proceeds independently of the negative feed-back regulation promoted by ammonium assimilation
Products: -
Products: -
?
ATP + H2O + NO3-/out
ADP + phosphate + NO3-/in
-
Substrates: high-affinity nitrate transport
Products: -
Products: -
?
ATP + H2O + NO3-/out
ADP + phosphate + NO3-/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + NO3-/out
ADP + phosphate + NO3-/in
-
Substrates: high-affinity nitrate transport
Products: -
Products: -
?
ATP + H2O + NO3-/out
ADP + phosphate + NO3-/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + NO3-/out
ADP + phosphate + NO3-/in
-
Substrates: rate-limiting step of nitrate-assimilation, nitrate transport activity is strictly dependent on active CO2-fixation. This positive C-control proceeds independently of the negative feed-back regulation promoted by ammonium assimilation
Products: -
Products: -
?
ATP + H2O + NO3-/out
ADP + phosphate + NO3-/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + NO3-/out
ADP + phosphate + NO3-/in
-
Substrates: inactivation of the enzyme at low temperatures limits growth at low temperatures
Products: -
Products: -
?
additional information
?
-
-
Substrates: nitrate transport through the cell membrane, important for nitrate accumulation in seeds, influences the kinetics of seed germination, not involved in the nitrate uptake in roots or in nitrate distribution within the vegetative organs
Products: -
Products: -
?
additional information
?
-
Substrates: efflux nitrate transport through the cell membrane, increased transport rates in acidic conditions without enhancement of transcript levels but increased enzyme level resulting from posttranscriptional processes, involved in nitrate distribution although this seems not be essential for correct plant growth in standard growth conditions
Products: -
Products: -
?
additional information
?
-
-
Substrates: nitrate transport through the cell membrane, important for NO3- signaling, which is important for the root architecture
Products: -
Products: -
?
additional information
?
-
-
Substrates: nitrate transport through the cell membrane
Products: -
Products: -
?
additional information
?
-
-
Substrates: nitrate transport through the cell membrane, NRT2.1 functions as the major nitrate uptake system and coordinates root development with external nitrate availability
Products: -
Products: -
?
additional information
?
-
-
Substrates: NRT1.7 is involved in nitrate remobilization from the old leaf
Products: -
Products: -
?
additional information
?
-
-
Substrates: NRT1.8, injected in Xenopus laevis oocytes, mediates low-affinity nitrate uptake
Products: -
Products: -
?
additional information
?
-
-
Substrates: high-affinity NO3- influx in the hni mutants, overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: NRT2.4 is a nitrate transporter functioning in the high-affinity range
Products: -
Products: -
?
additional information
?
-
-
Substrates: NRT1.9 is a low-affinity nitrate transporter
Products: -
Products: -
?
additional information
?
-
-
Substrates: nitrate transport through the cell membrane, BnNrt2.1 but not BnNrt1.1 expression level affects root architecture formation
Products: -
Products: -
?
additional information
?
-
-
Substrates: nitrate transport through the cell membrane
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme does not transport histidine
Products: -
Products: -
?
additional information
?
-
Substrates: NarK2 does not function as a nitrate/nitrite antiporter
Products: -
Products: -
?
additional information
?
-
-
Substrates: NarK2 does not function as a nitrate/nitrite antiporter
Products: -
Products: -
?
additional information
?
-
Substrates: NarK2 does not function as a nitrate/nitrite antiporter
Products: -
Products: -
?
additional information
?
-
-
Substrates: nitrate transport through the cell membrane
Products: -
Products: -
?
additional information
?
-
-
Substrates: nitrate and nitrite transport through the cell membrane, prefers nitrate over nitrite
Products: -
Products: -
?
additional information
?
-
-
Substrates: nitrate and nitrite transport through the cell membrane, essential for nitrogen supply
Products: -
Products: -
?
additional information
?
-
-
Substrates: important for nitrate/nitrite transport through the cell membrane, NarK2 protein is required as a nitrate/nitrite transporter under denitrifying conditions.
Products: -
Products: -
?
additional information
?
-
-
Substrates: nitrate transport through the cell membrane, reqiured for nitrate respiration under anoxic conditions
Products: -
Products: -
?
additional information
?
-
Substrates: Nrt2 is a Na+-dependent high-affnity nitrate transporter
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + H2O + nitrate-[nitrate-binding protein][side 1]
ADP + phosphate + nitrate[side 2] + [nitrate-binding protein][side 1]
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + nitrate/out
ADP + phosphate + nitrate/in
-
Substrates: NRT1.5 is a low-affinity, pH-dependent bidirectional nitrate transporter, that participates in root xylem loading of nitrate
Products: -
Products: -
?
ATP + H2O + nitrate/out
ADP + phosphate + nitrate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + nitrate/out
ADP + phosphate + nitrate/in
-
Substrates: NRT1.8 can transport nitrate and regulates nitrate removal from xylem. Nitrate distribution under Cd2+ stress is altered by NRT1.8
Products: -
Products: -
?
ATP + H2O + nitrate/out
ADP + phosphate + nitrate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + nitrate/out
ADP + phosphate + nitrate/in
Substrates: -
Products: -
Products: -
?
ATP + H2O + nitrate/out
ADP + phosphate + nitrate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + nitrate/out
ADP + phosphate + nitrate/in
-
Substrates: NRT1.5 is a low-affinity, pH-dependent bidirectional nitrate transporter, that participates in root xylem loading of nitrate
Products: -
Products: -
?
ATP + H2O + nitrate/out
ADP + phosphate + nitrate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + nitrate/out
ADP + phosphate + nitrate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + nitrate/out
ADP + phosphate + nitrate/in
Substrates: -
Products: -
Products: -
?
ATP + H2O + nitrate/out
ADP + phosphate + nitrate/in
Substrates: -
Products: -
Products: -
?
ATP + H2O + nitrate/out
ADP + phosphate + nitrate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + nitrate/out
ADP + phosphate + nitrate/in
Substrates: -
Products: -
Products: -
?
ATP + H2O + nitrate/out
ADP + phosphate + nitrate/in
Substrates: -
Products: -
Products: -
?
ATP + H2O + nitrate/out
ADP + phosphate + nitrate/in
Substrates: -
Products: -
Products: -
?
ATP + H2O + nitrate/out
ADP + phosphate + nitrate/in
Substrates: -
Products: -
Products: -
?
ATP + H2O + nitrate/out
ADP + phosphate + nitrate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + nitrate/out
ADP + phosphate + nitrate/in
-
Substrates: the enzyme transports both nitrate and nitrite with high affinity
Products: -
Products: -
?
ATP + H2O + nitrate/out
ADP + phosphate + nitrate/in
-
Substrates: the enzyme transports both nitrate and nitrite with high affinity
Products: -
Products: -
?
ATP + H2O + nitrate/out
ADP + phosphate + nitrate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + nitrate/out
ADP + phosphate + nitrate/in
-
Substrates: -
Products: -
Products: -
?
ATP + H2O + nitrite/out
ADP + phosphate + nitrite/in
Substrates: -
Products: -
Products: -
?
ATP + H2O + nitrite/out
ADP + phosphate + nitrite/in
-
Substrates: the enzyme transports both nitrate and nitrite with high affinity
Products: -
Products: -
?
ATP + H2O + nitrite/out
ADP + phosphate + nitrite/in
-
Substrates: the enzyme transports both nitrate and nitrite with high affinity
Products: -
Products: -
?
ATP + H2O + NO3-/out
ADP + phosphate + NO3-/in
-
Substrates: inactivation of the enzyme at low temperatures limits growth at low temperatures
Products: -
Products: -
?
ATP + H2O + NO3-/out
ADP + phosphate + NO3-/in
-
Substrates: rate-limiting step of nitrate-assimilation, nitrate transport activity is strictly dependent on active CO2-fixation. This positive C-control proceeds independently of the negative feed-back regulation promoted by ammonium assimilation
Products: -
Products: -
?
ATP + H2O + NO3-/out
ADP + phosphate + NO3-/in
-
Substrates: high-affinity nitrate transport
Products: -
Products: -
?
ATP + H2O + NO3-/out
ADP + phosphate + NO3-/in
-
Substrates: high-affinity nitrate transport
Products: -
Products: -
?
ATP + H2O + NO3-/out
ADP + phosphate + NO3-/in
-
Substrates: rate-limiting step of nitrate-assimilation, nitrate transport activity is strictly dependent on active CO2-fixation. This positive C-control proceeds independently of the negative feed-back regulation promoted by ammonium assimilation
Products: -
Products: -
?
ATP + H2O + NO3-/out
ADP + phosphate + NO3-/in
-
Substrates: inactivation of the enzyme at low temperatures limits growth at low temperatures
Products: -
Products: -
?
additional information
?
-
-
Substrates: nitrate transport through the cell membrane, important for nitrate accumulation in seeds, influences the kinetics of seed germination, not involved in the nitrate uptake in roots or in nitrate distribution within the vegetative organs
Products: -
Products: -
?
additional information
?
-
Substrates: efflux nitrate transport through the cell membrane, increased transport rates in acidic conditions without enhancement of transcript levels but increased enzyme level resulting from posttranscriptional processes, involved in nitrate distribution although this seems not be essential for correct plant growth in standard growth conditions
Products: -
Products: -
?
additional information
?
-
-
Substrates: nitrate transport through the cell membrane, important for NO3- signaling, which is important for the root architecture
Products: -
Products: -
?
additional information
?
-
-
Substrates: nitrate transport through the cell membrane
Products: -
Products: -
?
additional information
?
-
-
Substrates: nitrate transport through the cell membrane, NRT2.1 functions as the major nitrate uptake system and coordinates root development with external nitrate availability
Products: -
Products: -
?
additional information
?
-
-
Substrates: NRT1.7 is involved in nitrate remobilization from the old leaf
Products: -
Products: -
?
additional information
?
-
-
Substrates: NRT1.8, injected in Xenopus laevis oocytes, mediates low-affinity nitrate uptake
Products: -
Products: -
?
additional information
?
-
-
Substrates: high-affinity NO3- influx in the hni mutants, overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: NRT2.4 is a nitrate transporter functioning in the high-affinity range
Products: -
Products: -
?
additional information
?
-
-
Substrates: nitrate transport through the cell membrane, BnNrt2.1 but not BnNrt1.1 expression level affects root architecture formation
Products: -
Products: -
?
additional information
?
-
-
Substrates: nitrate transport through the cell membrane
Products: -
Products: -
?
additional information
?
-
-
Substrates: nitrate transport through the cell membrane
Products: -
Products: -
?
additional information
?
-
-
Substrates: nitrate and nitrite transport through the cell membrane, prefers nitrate over nitrite
Products: -
Products: -
?
additional information
?
-
-
Substrates: nitrate and nitrite transport through the cell membrane, essential for nitrogen supply
Products: -
Products: -
?
additional information
?
-
-
Substrates: important for nitrate/nitrite transport through the cell membrane, NarK2 protein is required as a nitrate/nitrite transporter under denitrifying conditions.
Products: -
Products: -
?
additional information
?
-
-
Substrates: nitrate transport through the cell membrane, reqiured for nitrate respiration under anoxic conditions
Products: -
Products: -
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Cd2+
-
the nrt1.8-1 mutant shows a nitrate-dependent Cd2+-sensitive phenotype, while wild-type NRT1.8 is upregulated by Cd2+ stress
Pb2+
exposure to Pb specifically stimulates Nrt1.1-mediated nitrate uptake. Nrt1.1-mediated reduction of Pb2+ uptake is associated with increased pH in the rhizosphere
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Cd2+
-
the nrt1.8-1 mutant shows a nitrate-dependent Cd2+-sensitive phenotype
NaCl
Q8YRV9; Q8YZ76; Q8YZ75
increase in salinity reduces the nitrate transport into wild-type cells
NH4+
-
the C-terminal domain of NrtC is involved in the ammonium-promoted inhibition of the nitrate/nitrite transporter
additional information
-
nitrate uptake is inhibited by addition of ammonium ions to the medium, while uptake of nitrite is not, results indicate that the transporter is not directly inhibited by ammonium ions
-
additional information
-
is degraded in the vacuole in response to glutamine, induced by nitrate and repressed by the preferred nitrogen sources ammonium or glutamine
-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
glycine betaine
Q8YRV9; Q8YZ76; Q8YZ75
recombinant glycine betaine synthesized within the cell protects nitrate transport from salt toxicity, and increases the efficiency of the nitrate transport system
additional information
-
the nrt1.8-1 mutant shows a nitrate-dependent Cd2+-sensitive phenotype, and nitrate transport by NRT1.8 is pH-dependent
-
NAR2
-
the two-transmembrane-domain protein is required for the nitrate transport activity of NRT2 transporters
-
NAR2
-
the two-transmembrane-domain protein is required for the nitrate transport activity of NRT2 transporters
-
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Anemia, Hypochromic
Nitrogen form-mediated ethylene signal regulates root-to-shoot K+ translocation via NRT1.5.
Anemia, Hypochromic
NRT1.1-Related NH4+ Toxicity Is Associated with a Disturbed Balance between NH4+ Uptake and Assimilation.
Hypersensitivity
Ammonium aggravates salt stress in plants by entrapping them in a chloride over-accumulation state in an NRT1.1-dependent manner.
Infections
Abscisic acid and lateral root organ defective/NUMEROUS INFECTIONS AND POLYPHENOLICS modulate root elongation via reactive oxygen species in Medicago truncatula.
Infections
Alternaria Brassicae Induces Systemic Jasmonate Responses in Arabidopsis Which Travel to Neighboring Plants via a Piriformsopora Indica Hyphal Network and Activate Abscisic Acid Responses.
Iron Deficiencies
The nitrate transporter NRT1.1 is involved in iron deficiency responses in Arabidopsis
Plant Diseases
A deletion in NRT2.1 attenuates Pseudomonas syringae-induced hormonal perturbation, resulting in primed plant defenses.
Starvation
A central role for the nitrate transporter NRT2.1 in the integrated morphological and physiological responses of the root system to nitrogen limitation in Arabidopsis.
Starvation
Arabidopsis NRT1.5 Mediates the Suppression of Nitrate Starvation-Induced Leaf Senescence by Modulating Foliar Potassium Level.
Starvation
Comparative genome and transcriptome analysis unravels key factors of nitrogen use efficiency in Brassica napus L.
Starvation
eCALIBRATOR: A Comparative Tool to Identify Key Genes and Pathways for Eucalyptus Defense Against Biotic Stressors.
Starvation
Genome-wide identification and analysis of high-affinity nitrate transporter 2 (NRT2) family genes in rapeseed (Brassica napus L.) and their responses to various stresses.
Starvation
Improving nitrogen use efficiency by manipulating nitrate remobilization in plants.
Starvation
Reciprocal regulation between AtNRT2.1 and AtAMT1.1 expression and the kinetics of NH(4)(+) and NO(3)(-) influxes.
Starvation
Repression of Nitrogen Starvation Responses by Members of the Arabidopsis GARP-Type Transcription Factor NIGT1/HRS1 Subfamily.
Starvation
The Arabidopsis Nitrate Transporter NRT2.4 Plays a Double Role in Roots and Shoots of Nitrogen-Starved Plants.
Starvation
The Arabidopsis nitrate transporter NRT2.5 plays a role in nitrate acquisition and remobilization in nitrogen-starved plants.
Starvation
The AraC-type regulator RipA represses aconitase and other iron proteins from corynebacterium under iron limitation and is itself repressed by DtxR.
Starvation
The expression patterns and putative function of nitrate transporter 2.5 in plants.
Starvation
The Transcription Factor NIGT1.2 Modulates Both Phosphate Uptake and Nitrate Influx during Phosphate Starvation in Arabidopsis and Maize.
Starvation
[Separation of the up-regulated genes under nitrogen starvation from Phaeodactylum tricornutum by suppression subtractive hybridization technology]
Tuberculosis
A single-nucleotide mutation in the -10 promoter region inactivates the narK2X promoter in Mycobacterium bovis and Mycobacterium bovis BCG and has an application in diagnosis.
Tuberculosis
Differences in nitrate reduction between Mycobacterium tuberculosis and Mycobacterium bovis are due to differential expression of both narGHJI and narK2.
Tuberculosis
Expression of the Mycobacterium tuberculosis acr-coregulated genes from the DevR (DosR) regulon is controlled by multiple levels of regulation.
Tuberculosis
Interaction of DevR with multiple binding sites synergistically activates divergent transcription of narK2-Rv1738 genes in Mycobacterium tuberculosis.
Tuberculosis
Mutational analysis of the respiratory nitrate transporter NarK2 of Mycobacterium tuberculosis.
Tuberculosis
Regulation of nitrate reductase activity in Mycobacterium tuberculosis by oxygen and nitric oxide.
Tuberculosis
Role of narK2X and narGHJI in hypoxic upregulation of nitrate reduction by Mycobacterium tuberculosis.
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.15
nitrate-[nitrate-binding protein][side 1]
-
pH not specified in the publication, temperature not specified in the publication
-
0.49
nitrate-[nitrate-binding protein][side 1]
-
pH not specified in the publication, temperature not specified in the publication
-
2.5
nitrate-[nitrate-binding protein][side 1]
-
pH not specified in the publication, temperature not specified in the publication
-
0.0416
nitrate/out
recombinant enzyme in frog oocytes, pH 5.5, temperature not specified in the publication
additional information
additional information
-
uptake kinetics of recombinant NRT1.9 in NRT1.9-injected oocytes, pH 5.5
-
additional information
nitrate-[nitrate-binding protein][side 1]
in Xenopus oocytes, NRT2.4 alone without partner protein NAR2 coexpression shows biphasic kinetics at a wide concentration range, with high- and low-affinity KM values of 0.15 and 4 mM, respectively. pH not specified in the publication, temperature not specified in the publication
-
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
treatments with the ethylene precursor, aminocyclopropane carboxylic acid and the ethylene biosynthesis inhibitor, aminoethoxyvinylglycine are found to affect nitrate uptake
-
-
brenda
ssp. chinensis Makino, cultivar Suzhouqing, gene BcNRT1
UniProt
brenda
cv. Gatersleben, boron is found to affect nitrate uptake, boron deficiency causes decrease in nitrate uptake
-
-
brenda
Q8YRV9 i.e. subunit NrtA, Q8YZ76 i.e. NrtC, Q8YZ75 i.e. subunit NrtD
Q8YRV9; Q8YZ76; Q8YZ75
UniProt
brenda
different strains which are derivatives of the NCYC495 leu2 ura3 strain, enzyme is highly expressed in media containing nitrate as sole nitrogen source, addition of glutamine or ammonium decreased nitrate uptake and enzyme level
-
-
brenda
wild type and deletion mutants, NarK1 deletion does not affect nitrate uptake while NarK2 deletion causes strongly reduced nitrate uptake rates
-
-
brenda
ecotype Wassilewskija, NRT2.1 is up-regulated by nitrogen starvation, mutation of NRT2.1 or NRT2.2 has no significant consequences on the development of root growth under nonlimiting nitrogen conditions, in NRT2.1-dysfunction mutants the length of lateral roots is increased by low nitrogen supply
-
-
brenda
formerly unidentified protein; light and N-status does not affect enzyme level
UniProt
brenda
Heynh., deficiency mutant and transgenic derivatives of this mutant
-
-
brenda
strain PCC7942, ABC-type bispecific nitrate/nitrite transporter is encoded by the four genes nrtA, -B, -C, and -D
-
-
brenda
strain PCC7942, ABC-type bispecific nitrate/nitrite transporter is encoded by the four genes nrtA, -B, -C, and -D
-
-
brenda
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
histochemical analysis shows that NRT1.8 is expressed predominantly in xylem parenchyma cells within the vasculature
brenda
-
highly expressed in dry seeds, especially at the end of seed maturation, weak expression in the surrounding endosperm
brenda
additional information
-
NRT1.9 is expressed in the companion cells of the phloem
brenda
-
NRT1.9 is a transporter expressed in the companion cells of root phloem
brenda
-
NRT1.7 is expressed in the phloem of the leaf minor vein. NRT1.7 is predominantly expressed in old leaves
brenda
-
NRT1.4 and NRT1.7, the latter is expressed in the phloem of minor veins in older leaves
brenda
-
NRT1.7 is expressed in the phloem of the leaf minor vein
brenda
-
NRT1.9 is expressed in the companion cells of the phloem, NRT1.7 is expressed in the phloem of minor veins in older leaves
brenda
-
NRT2.4 expression in lateral root epidermal cells. NRT2.1 expression in the older part of the primary root regardless of nitrogen status
brenda
-
CHL1, i.e. NRT1.1, is expressed in root epidermis, cortex, and endodermis. NRT1.8, a low-affinity nitrate transporter located in the plasma membrane, is expressed dominantly in the xylem parenchyma cells of roots
brenda
-
lateral, in the epidermis of lateral roots and in or close to the shoot phloem, spatiotemporal expression pattern of NRT2.4 in roots, overview
brenda
NRT2.1 is the major component of the root high affinity nitrate transport system
brenda
growing region of the root, root hair, and root stele, expression during lateral root formation and during the formation and emergence of the lateral root primordia
brenda
MtNRT1.3 expression is developmentally regulated in roots, with increasing expression after completion of germination in N-free medium
brenda
-
expressed in the pericycle region of primary roots and lateral roots, and in lateral root primordia and tips
brenda
-
MtNRT1.3 expression is developmentally regulated in roots, with increasing expression after completion of germination in N-free medium
-
brenda
-
MtNRT1.3 expression is developmentally regulated in roots, with increasing expression after completion of germination in N-free medium
-
brenda
NRT2.4 is expressed mainly in the base of lateral root primordia
brenda
-
NRT2.4 expression in shoots is limited to the primary veins of leaves and inflorescences and is adjacent to the phloem parenchyma
brenda
NRT2.4 is expressed mainly in vascular tissues
brenda
-
histochemical analysis shows that NRT1.8 is expressed predominantly in xylem parenchyma cells within the vasculature
brenda
expresses preferentially in xylem parenchyma cells of vasculature tissues
brenda
additional information
-
expression of NRT1.7 is diurnally regulated, quantitative RT-PCR analysis of NRT1.7 expression, overview
brenda
additional information
-
in nitrate-containing media, NRT2.4 expression is detected at low levels in roots and undetectable in shoots, but is induced after 3 days of nitrogen starvation 12fold in roots and detectable in shoots. NRT2.1 expression is high in roots in the presence of external nitrate and, after a transient increase, is relatively unchanged after 3 days of nitrogen starvation
brenda
additional information
-
quantitative RT-PCR NRT2.4 expression analysis
brenda
additional information
-
quantitative RT-PCR NRT1.9 expression analysis
brenda
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
accumulation of enzyme upon acid load or medium acidification
brenda
-
part of a mobilizable respiratory island for nitrate respiration
brenda
-
subcellular localization study, overview
brenda
presence of 12 transmembrane domains is predicted
brenda
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
evolution
malfunction
metabolism
physiological function
additional information
evolution
-
the enzyme belongs to the NRT1/PTR family. Of 16 members characterized, some transport nitrate and some transport dipeptides. With the exception of Arabidopsis thaliana CHL1 (AtNRT1.1) and Mycobacterium tuberculosis NRT1.3, which are dual-affinity nitrate transporters, most of the NRT1 nitrate transporters characterized are low-affinity nitrate transporters. Most nitrate and peptide transporters characterized in the NRT1/PTR family are proton-coupled transporters. all NRT2 transporters isolated from Aspergillus, Chlamydomonas, and higher plants transport nitrate. It is believed that the NRT2s are also proton-coupled transporters
evolution
-
the enzyme belongs to the NRT1/PTR family. Of 16 members characterized, some transport nitrate and some transport dipeptides. With the exception of Arabidopsis thaliana CHL1 (AtNRT1.1) and Mycobacterium tuberculosis NRT1.3, which are dual-affinity nitrate transporters, most of the NRT1 nitrate transporters characterized are low-affinity nitrate transporters. Most nitrate and peptide transporters characterized in the NRT1/PTR family are proton-coupled transporters. all NRT2 transporters isolated from Aspergillus, Chlamydomonas, and higher plants transport nitrate. It is believed that the NRT2s are also proton-coupled transporters
evolution
-
the enzyme belongs to the NRT1/PTR family. Of 16 members characterized, some transport nitrate and some transport dipeptides. With the exception of Arabidopsis thaliana CHL1 (AtNRT1.1) and Mycobacterium tuberculosis NRT1.3, which are dual-affinity nitrate transporters, most of the NRT1 nitrate transporters characterized are low-affinity nitrate transporters. Most nitrate and peptide transporters characterized in the NRT1/PTR family are proton-coupled transporters. all NRT2 transporters isolated from Aspergillus, Chlamydomonas, and higher plants transport nitrate. It is believed that the NRT2s are also proton-coupled transporters
evolution
-
ten putative NRT2 and three putative NRT3 genes are found in Hordeum vulgare. All NRT2/3 genes are located on chromosomes 3H, 5H, 6H or 7H. The HvNRT2/3 genes display various expression patterns at selected developmental stages and are induced in the roots by both low and high nitrogen levels
evolution
-
a total of 331 NPF genes (113 homoeologous groups) encoding membrane transporters involved in the transport of a large variety of substrates including nitrate and peptides are identified in wheat. The chromosomal location of the NPF genes is unevenly distributed. Wheat NPF genes are closely clustered with Arabidopsis thaliana, Brachypodium, and rice orthologues, and subdivided into eight subfamilies
evolution
-
homologs of NrtS are present in a limited number of eubacteria including mostly cyanobacteria and proteobacteria
evolution
-
homologs of NrtS are present in a limited number of eubacteria including mostly cyanobacteria and proteobacteria
evolution
-
homologs of NrtS are present in a limited number of eubacteria including mostly cyanobacteria and proteobacteria
evolution
-
homologs of NrtS are present in a limited number of eubacteria including mostly cyanobacteria and proteobacteria
-
malfunction
-
nrt2.1 mutants show reduced susceptibility to the bacterial pathogen Pseudomonas syringae pv tomato DC3000. In NRT1.7 mutants more nitrate accumulates in older leaves, less 15NO3-dropped on the older leaves can be transported to younger leaves, and less nitrate is detected in the phloem sap of the older leaves. Nrt1.8 mutant shows a nitrate-dependent cadmium-sensitive phenotype and, compared with the wild type, an increased amount of cadmium is transported to the shoot. In the Arabidopsis nar2.1 mutant, the disappearance of NRT2.1 protein in the membrane fraction suggests that NAR2.1 is required for the plasma membrane targeting, and/or the protein stability, of NRT2.1. Nrt1.8 mutants show increased nitrate content in xylem sap and increased root-to-shoot nitrate translocation
malfunction
-
functional disruption of NRT1.5 enhances tolerance to salt, drought and cadmium stresses, also nitrate as well as Na+ and Cd2+ levels are significantly increased in nrt1.5 roots. Genes including NHX1, SOS1, P5CS1, RD29A, AtPCS1 and NRT1.8, important in stress response pathways, are steadily upregulated in nrt1.5 mutant plants
malfunction
-
in N-starved nrt2.4 mutants, nitrate uptake under low external supply and nitrate content in shoot phloem exudates is decreased. In the absence of NRT2.1 and NRT2.2, loss of function of NRT2.4 (triple mutants) has an impact on biomass production under low nitrate supply, phenotypes, overview
malfunction
-
in nrt1.9 mutants, nitrate content in root phloem exudates is decreased, and downward nitrate transport is reduced. Under high nitrate conditions, the nrt1.9 mutant shows enhanced root-to-shoot nitrate transport and plant growth, phenotypes, overview
malfunction
-
mutants lacking AtNAR2.1 have virtually no high-affinity nitrate transport system capacity and exhibit extremely poor growth on low nitrate as the sole source of nitrogen. Near-normal growth and nitrate transport in the mutant are restored by transformation with myc-tagged AtNAR2.1 cDNA
malfunction
-
reduction of nitrate transporter NRT2.5 expression results in a decrease in high-affinity nitrate uptake without impacting low-affinity uptake
malfunction
-
enzyme nockout decreases rice growth and potassium concentration in xylem sap, root, culm, and sheath, but increases the shoot:root ratio of tissue potassium under higher nitrate
malfunction
-
functional disruption of NRT1.1 enhances resistance to iron deficiency stress
malfunction
-
a loss-of-function mutation in NPF2.3 results in decreased root-to-shoot nitrate translocation and reduced shoot nitrate content in plants grown under salt stress
metabolism
-
NRT1.1 is involved in a mechanism connecting nutrient and hormone signaling during organ development, overview
metabolism
-
NRT1.8 is the only nitrate assimilatory pathway gene that is strongly upregulated by Cd2+ stress in roots. NRT1.8-regulated nitrate distribution plays an important role in Cd2+ tolerance
metabolism
-
nitrate uptake is regulated at both the transcriptional and post-transcriptional level
metabolism
-
nitrate reallocation to roots might be a common response to stresses and is coordinately regulated by NRT1.8 and NRT1.5 genes, overview
metabolism
NRT2.1 protein stability is regulated in response to nitrogen nutrition availability. In seedlings transferred to nitrate-limited conditions, the apparent half-life of NRT2.1 in roots increases from 3 to 9 h. The stabilization of NRT2.1 protein occurs rapidly, even prior to the transcriptional stimulation of NRT2.1
physiological function
-
the NRT1.1 nitrate transporter is crucial for nitrate signaling governing root growth, and acts as a nitrate sensor. NRT1.1 also facilitates uptake of the phytohormone auxin. Moreover, nitrate inhibits NRT1.1-dependent auxin uptake, suggesting that transduction of nitrate signal by NRT1.1 is associated with a modification of auxin transport. Mutation of NRT1.1 enhances both auxin accumulation in lateral roots and growth of these roots at low, but not high, nitrate concentration. NRT1.1 represses lateral root growth at low nitrate availability by promoting basipetal auxin transport out of these roots NRT1.1, mechanism, overview
physiological function
-
NRT1 transporter NRT1.7 is a low-affinity nitrate transporter, and involved in nitrate remobilization from the old leaf. Internal nitrate remobilization between leaves was important for plants to cope with nitrogen deficiency and the importance of enhanced nitrogen use efficiency for maximum growth. And NRT1.7 is responsible for phloem loading of nitrate in the source leaf to allow nitrate transport out of older leaves and into younger leaves
physiological function
-
nitrate transporter NRT1.8 functions in nitrate removal from the xylem sap and mediates cadmium tolerance
physiological function
-
pNRT2.1 is involved in high-affinity root uptake, and is a major target of this N signaling mechanism
physiological function
-
NRT2.1 is a prominent high-affinity nitrate transporter that functions at low external nitrate concentrations and plays an additional role in lateral root initiation that is independent of this transport function. NRT2.4 is a high-affinity nitrate transporter important in both root uptake and phloem loading and that its spatial and temporal expression complements that of NRT2.1
physiological function
-
the NRT2.1- NAR2.1 complex has dual effects on lateral root development. In addition to being a transporter involved in nitrate uptake, CHL1 also functions as a nitrate sensor, regulating a transcriptional response called the primary nitrate response with roles in regulating root architecture. NRT1.4, a low-affinity nitrate transporter, plays a role in regulating leaf nitrate homeostasis and leaf development, NRT1.4 expressed in the petiole could affect lamina nitrate content and lamina growth. NRT1 transporters NRT1.8 and NRT1.9 are involved in regulating root-to-shoot nitrate translocation, both NRT1.8 and NRT1.9 are negative regulators of root-to-shoot nitrate transport but through different mechanisms. The function of NRT1.8 in removing nitrate from xylem sap also allows Cd2+ to stay in the roots, and consequently enhances Cd2+ tolerance. CHL1, i.e. NRT1.1, is a dual-affinity nitrate transporter mediating both the high-affinity transport system and the low-affinity transport system. The switch between the two affinities is controlled by phosphorylation at the T101 residue between the second and third transmembrane domains, this phosphorylation is regulated by the calcineurin B-like-interacting protein kinase CIPK23. Dual-affinity transport activity is also exhibited by the potassium transporter KUP and the nitrate transporter MtNRT1.3. NRT1.8 functions in removing nitrate from the xylem sap back into the root cells
physiological function
-
NRT1.5 functions to mediate nitrate reallocation to roots, stress-responsive gene expression and metabolism, and consequently salt, drought and Cd2+ tolerance. NRT1.5 is involved in nitrate allocation to roots and the consequent tolerance to several stresses, in a mechanism probably shared with NRT1.8. NRT1.5 works together with NRT1.8 to fine-tune nitrate long-distance transport from roots to shoots
physiological function
nitrate transporter NRT1.3 is involved in the control of primary root growth and NO3- sensing acting in the response to N limitation, which increases the ability of the plant to acquire NO3- under N-limiting conditions
physiological function
-
the Arabidopsis thaliana high affinity range nitrate transporter NRT2.4 plays a double role in roots and shoots of nitrogen-starved plants
physiological function
-
NRT1.9 facilitates loading of nitrate into the root phloem and enhance downward nitrate transport in roots, expression of NRT1.9 in root companion cells
physiological function
-
nitrate transporter NRT2.5 plays a role in nitrate acquisition and remobilization in nitrogen-starved plants by ensuring the efficient uptake of nitrate collectively with enzyme forms NRT2.1, NRT2.2 and NRT2.4 and by taking part in nitrate loading into the phloem during nitrate remobilization
physiological function
-
enzyme NPF6.8 s a major contributor to the inducible component of the low-affinity transport system and plays a role of in the primary nitrate response
physiological function
-
the enzyme plays a major role in post-flowering nitrate uptake
physiological function
-
the enzyme functions in acquisition and long-distance transport of nitrate and plays an important role in maintaining nitrate-mediated growth and development in rice. Low-affinity nitrate acquisition of roots is increased by enzyme overexpression
physiological function
-
under low N condition, enzyme NRT1.1b accumulates more nitrogen in plants and improves rice growth
physiological function
-
the enzyme is involved in iron deficiency responses
physiological function
-
the enzyme contributes to nitrate translocation to shoots under salt stress
physiological function
-
the nitrate transporter NPF7.3/NRT1.5 is involved in lateral root development under potassium deprivation. The enzyme drives root-to-shoot transport of NO3- and is also involved in root-to-shoot translocation of potassium under low NO3- nutrition
physiological function
-
plant nitrate transporter 1/ peptide transporter family (NPF) proteins may function as gibberellic acid transporters. A screen of all Arabidopsis thaliana NPF proteins reveals transport activity towards six gibberellic acids and identifies distinct residues within the substrate-binding cavity that are shared between gibberellic acid transporting NPF proteins. From subclade NPF4, only NPF4.1 and NPF4.6 transport nonpermeating gibberellic acids, and NPF4.6 accumulates only about 15% of gibberellic acids compared to NPF4.1 levels. NPF3.1 imports approximately 40% of NPF4.1 levels. NPF1.1, NPF2.3, NPF2.4, NPF2.7, NPF2.12, and NPF2.13 import gibberellic acids to approximately 10% to 15% of NPF4.1 levels
physiological function
-
overexpression of isofrm NRT2.1 improves the yield related traits in Arabidopsis thaliana
physiological function
a loss-of function mutant of nitrate transporter gene NRT2.1 lacks low red:far red light-induced lateral root reduction and its root growth is hypersensitive to low nitrate. Gene ELONGATED HYPOCOTYL5 (HY5) plays an important role in the root response to low red:far red light. Low red:far red light increases NRT2.1 expression and low nitrate enhances HY5 expression. HY5 also affects NRT2.1 expression
physiological function
nitrate allocation mediated by NPF7.9 is essential for balancing rice growth and stress tolerance. Nitrate allocation from roots to shoots is decreased in Npf7.9 mutants. Biomass, grain yield, and nitrogen use efficiency decrease in the mutant dependent on nitrate availability. In Npf7.9 mutants, less K accumulates in mutant shoots than in wild-type when supplied with 1 mM NH4NO3, but not with 0.25 mM NH4NO3
physiological function
when high-NH4+ medium is supplemented with low concentrations of NO3-, Nrt1.1 mutant plants show a NH4+ hyper-sensitive phenotype. Mutation in Nrt1.1 causes enhanced medium acidification under high-NH4+/low-NO3- condition. Nrt1.1 interact with NO3- efflux channel Slah3 to form a transporter-channel complex. Slah3 appears to affect NO3- influx while Nrt1.1 influences NO3- efflux
physiological function
-
the sensitivity of the primary root tip to nitrate is strongly linked to the integrity of MtNPF6.8 which acts as a master mediator of the nitrate signal involved in the control of the root system architecture. The Npf6.8-3 mutant behaves as the wild-type grown without nitrate. 13 transcripts and no protein are differently accumulated in the primary root tip of the Npf6.8-3 mutant line in response to nitrate
physiological function
In Xenopus oocytes, NRT2.4 alone without partner protein NAR2 coexpression facilitates nitrate uptake showing biphasic kinetics at a wide concentration range, with high- and low-affinity KM values of 0.15 and 4 mM, respectively. NRT2.4 does not have nitrate efflux or indole-3-acetic acid influx activity. Knockout of NRT2.4 decreases lateral root number and length, and the total N uptake per plant at both 0.25 and 2.5 mM NO3? levels. Knockout of NRT2.4 in the shoots decreases the growth and NO3--N distribution. Knockout of NRT2.4 does not affect rice growth and N uptake under conditions without N or with only NH4+ supply
physiological function
under alternating wet and dry condition, wild-type plants show a 31% reduction in grain yields compared to waterlogged condition. The overexpression of NRT2.1 recovers this loss, and NRT2.1 transgenic lines display higher grain yields than wild-type plants. Transgenic lines display 60% higher grain Mn under alternating wet and dry condition and approximately 30% higher Mn in the grain in waterlogged condition. The overexpression of NRT2.1 does not alter Mg and Fe in the seeds in either growth condition
physiological function
NRT1.5 mutants display conspicuously longer primary roots along with a significantly reduced lateral root density under phosphate-deficient conditions than does the wild-type plants. Ethylene synthesis antagonist Co2+ partly rescues this phenotype. The expression of important phosphate starvation-induced genes, is significantly higher in the NRT1.5 mutants than in wild-type plants under phospahte-starvation conditions. NRT1.5 mutant plants retain higher tissue phosphate concentrations
physiological function
-
overexpression of NrtS from an inducible promoter confers nitrate uptake activity on the nitrate transport-less NA4 mutant of Synechococcus elongatus
physiological function
-
NrtS1 and NrtS2 form a heteromeric transporter complex. Coexpression of the two genes in nitrate transport-less NA4 mutant of Synechococcus elongatus results in nitrate uptake, while expression of either of the two results in low-affinity nitrate uptake activity with Km values of >3 mM
physiological function
expression of Nrt2-GFP together with the accessory protein NAR2 (Zosma63g00220) in Nicotiana benthamiana leaves displays four-fold higher fluorescence intensity than single expression Nrt2-GFP
physiological function
loss of function of isoform Nrt1.1 results in greater Pb toxicity and higher Pb accumulation in nitrate-sufficient growth medium.In contrast, no difference is seen between wild-type plants and null-mutants for isoforms Nrt1.2, Nrt2.1, Nrt2.2, Nrt2.4, and Nrt2.5. Rhizosphere acidification, which favors Pb entry to roots by increasing its availability, is prevented when Nrt1.1 is functional and both NO3- and NH4+ are present in the medium
physiological function
-
overexpression of NrtS from an inducible promoter confers nitrate uptake activity on the nitrate transport-less NA4 mutant of Synechococcus elongatus
-
physiological function
-
nitrate transporter NRT1.3 is involved in the control of primary root growth and NO3- sensing acting in the response to N limitation, which increases the ability of the plant to acquire NO3- under N-limiting conditions
-
physiological function
-
nitrate transporter NRT1.3 is involved in the control of primary root growth and NO3- sensing acting in the response to N limitation, which increases the ability of the plant to acquire NO3- under N-limiting conditions
-
additional information
-
in nrt1.7 mutants, more nitrate is present in the older leaves, less 15NO3- spotted on old leaves is remobilized into N-demanding tissues, and less nitrate is detected in the phloem exudates of old leaves. Nrt1.7 mutants show growth retardation when external nitrogen is depleted
additional information
-
functional disruption of NRT1.8 significantly increased the nitrate concentration in xylem sap
additional information
-
a membrane protein, NAR2, is required for the nitrate transport activity of NRT2 transporters
additional information
-
a membrane protein, NAR2, is required for the nitrate transport activity of NRT2 transporters
additional information
MtNRT1.3 is a dual-affinity NO3- transporter
additional information
-
expression of NRT2.4 and NRT2.1 is differentially regulated in young seedlings in response to N availability
additional information
-
AtNRT2.1 is a polypeptide of the Arabidopsis thaliana two-component inducible high-affinity nitrate transport system, IHATS, formed by AtNRT2.1 and AtNAR2.1, i.e AtNRT3.1.The monomeric form of AtNRT2.1 is the most abundant form, but the complex, rather than monomeric AtNRT2.1, is the form that is active in IHATS nitrate transport
additional information
-
MtNRT1.3 is a dual-affinity NO3- transporter
-
additional information
-
MtNRT1.3 is a dual-affinity NO3- transporter
-
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). NRTC_SYNE7
Synechococcus elongatus (strain ATCC 33912 / PCC 7942 / FACHB-805)
659
0
72346
Swiss-Prot
-
NRTD_SYNE7
Synechococcus elongatus (strain ATCC 33912 / PCC 7942 / FACHB-805)
274
0
30366
Swiss-Prot
Mitochondrion (Reliability: 5)
NRTD_SYNY3
Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa)
266
0
29920
Swiss-Prot
-
NRTC_SYNY3
Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa)
670
0
75101
Swiss-Prot
other Location (Reliability: 2)
C7SFH0_9AGAR
510
0
54856
TrEMBL
other Location (Reliability: 3)
D5LQ68_MEDTR
588
11
64884
TrEMBL
Secretory Pathway (Reliability: 1)
NRT21_ORYSJ
533
0
57230
Swiss-Prot
Secretory Pathway (Reliability: 1)
NARK2_MYCTU
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
395
0
41109
Swiss-Prot
Secretory Pathway (Reliability: 1)
PTR7_ARATH
590
0
64922
Swiss-Prot
Secretory Pathway (Reliability: 1)
Q0DYI7_ORYSJ
610
0
66037
TrEMBL
other Location (Reliability: 1)
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PDB
SCOP
CATH
UNIPROT
ORGANISM
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
26000
-
2 * 48000, AtNRT2.1, SDS-PAGE, + 2 * 26000, myc-tagged AtNAR2.1, SDS-PAGE, the functional unit for high-affinity nitrate influx may be a tetramer consisting of two subunits each of AtNRT2.1 and AtNAR2.1
45000
-
His-tagged NrtA1, silver staining and immunoblotting of two-dimensional gel electrophoresis
47300
-
x * 47300, NarK1, calculated from the deduced amino acid sequence
48000
-
2 * 48000, AtNRT2.1, SDS-PAGE, + 2 * 26000, myc-tagged AtNAR2.1, SDS-PAGE, the functional unit for high-affinity nitrate influx may be a tetramer consisting of two subunits each of AtNRT2.1 and AtNAR2.1
50600
-
x * 50600, NarK2, calculated from the deduced amino acid sequence
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
tetramer
-
2 * 48000, AtNRT2.1, SDS-PAGE, + 2 * 26000, myc-tagged AtNAR2.1, SDS-PAGE, the functional unit for high-affinity nitrate influx may be a tetramer consisting of two subunits each of AtNRT2.1 and AtNAR2.1
additional information
additional information
Q8YRV9; Q8YZ76; Q8YZ75
subunit NrtA protein consists of 427 amino acids, the majority of which are hydrophobic, and contains a Tat (twin-arginine translocation) signal profile. The NrtC subunit of 657 amino acids contains two hydrophobic distinct domains, its C-terminal may serve as a solute binding domain and N-terminal as ATP binding domain. Subunit NrtD consists of 277 amino acids and its N-terminal is an ATP binding motif
additional information
-
ATP-binding cassette-type ATPase, NrtC and NrtD constitute the membrane transporter complex, NrtB is a hydrophobic protein with five putative membrane-spanning segments, the substrate-binding protein NrtA is tightly bound to the cytoplasmic membrane, an NrtA-like extra domain is linked to one of the ATP-binding subunits
additional information
-
nrtB, nrtC and nrtD encode proteins of 279, 659 and 274 amino acids
additional information
-
the nrtB, nrtC and nrtD genes encode membrane components of the ATP-binding cassette transporter, the 45000 Da nrtA gene product is a nitrate- and nitrite-binding lipoprotein
additional information
-
ATP-binding cassette-type ATPase, NrtC and NrtD constitute the membrane transporter complex, NrtB is a hydrophobic protein with five putative membrane-spanning segments, the substrate-binding protein NrtA is tightly bound to the cytoplasmic membrane, an NrtA-like extra domain is linked to one of the ATP-binding subunits
-
additional information
-
the nrtB, nrtC and nrtD genes encode membrane components of the ATP-binding cassette transporter, the 45000 Da nrtA gene product is a nitrate- and nitrite-binding lipoprotein
-
additional information
-
nrtB, nrtC and nrtD encode proteins of 279, 659 and 274 amino acids
-
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POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phosphoprotein
additional information
-
nitrogen sources trigger Ynt1 ubiquitinylation, Ynt1 becomes modified with ubiquitin during down-regulation as a result of nitrate assimilation or the shift to a preferred nitrogen source
phosphoprotein
the mode of action ofNRT1.1 is controlled by phosphorylation of a key residue Thr101
phosphoprotein
phosphorylation at residue Ser28 of NRT2.1 is involved in regulating its stability. Substitution of Ser28 by alanine results in unstable NRT2.1
phosphoprotein
-
phosphorylation sites at the central cytosolic domain of the enzyme, not involved in the down-regulation of the enzyme
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
apo and nitrate-bound enzyme, hanging drop vapor diffusion method, using 22% (w/v) PEG 400, 0.05 M sodium citrate pH 4.5, 0.07 M sodium chloride and 1.5% (w/v) PEG 600
hanging drop vapor diffusion method, using 100 mM sodium acetate, pH 4.5, 30% PEG300 and 3% (w/v) 2-methyl-2,4-pentanediol
homology modeling and docking analysis. Amino acids Lys251, Lys295and Arg298, Ser290, Asp246, and Pro48 of the NrtA protein interact in docking with nitrate
Q8YRV9; Q8YZ76; Q8YZ75
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
H356A
the mutation results in complete loss of nitrate binding ability
S28A
substitution results in unstable NRT2.1, and a mutant strain fails to complement the growth-restricted phenotype of a Nrt2.1 mutant when a low concentration of nitrate is the sole nitrogen source
T101A
-
inactivation of the high-affinity component of the transporter by preventing phosphorylation at Thr101
T101D
the variant shows increased nitrate uptake of approximately 2.8fold compared to the wild type enzyme
F265A
the mutant retains very weak binding to nitrate but not to nitrite
N173A
the mutant retains some binding to substrate, albeit drastically reduced in comparison to the wild type
R303A
the mutation completely abrogates binding to nitrate or nitrite
Y261A,
the mutation completely abrogates binding to nitrate or nitrite
D267G
the mutant shows reduced activity compared to the wild type enzyme
F114S
the mutant shows reduced activity compared to the wild type enzyme
F19S
the mutant shows reduced activity compared to the wild type enzyme
G140S
the mutant shows reduced activity compared to the wild type enzyme
G315S
the mutant shows reduced activity compared to the wild type enzyme
G69S
the mutant shows reduced activity compared to the wild type enzyme
P50S
the mutant shows reduced activity compared to the wild type enzyme
P84T
the mutant shows reduced activity compared to the wild type enzyme
F19S
-
the mutant shows reduced activity compared to the wild type enzyme
-
K243R/K253R/K270R
-
no ubiquitinylation, strongly reduced down-regulation in response to glutamine
additional information
additional information
construction of a knockout mutant, no effect on plant growth, no changes in nitrate uptake upon acid load or medium acidification
additional information
-
mutation of the NRT1.5 nitrate transporter causes defective root-to-shoot nitrate transport, knockdown or knockout mutations of NRT1.5 reduces the amount of nitrate transported from the root to the shoot, Root and shoot nitrate contents of antisense NRT1.5 plants, overview. T-DNA-tagged mutants nrt1.5-1 and nrt1.5-2 are defective in long-distance transport of nitrate but not of sulfate or phosphate
additional information
-
a line expressing the auxin-inducible DR5::GUS reporter gene is crossed with the chl1-5 knockout mutant for NRT1.1. Mutation of NRT1.1 does not affect expression of DR5::GUS in plants supplied with 1 mM NO3, but dramatically increases it in plants either grown in the absence of NO3- or supplied with a low external NO3- concentration. Absence of a functional NRT1.1 transporter prevents the decrease of DR5::GUS expression in response to removal or lowered supply of NO3-, leading to a high DR5 activity regardless of the presence of an N source, phenotype, overview
additional information
-
isolation of three high nitrogen insensitive mutants, belonging to three genetic loci and related to single and recessive mutations, that display reduced downregulation of both NRT2.1 expression and high-affinity NO3- influx under repressive conditions. associated with an almost complete suppression of systemic repression of pNRT2.1 activity by high N status of the whole plant. The three hni mutants define a class of N signaling mutants specifically impaired in the systemic feedback repression of root NO3- uptake, molecular effects of the mutations and phenotypes, detailed overview. pNRT2.1::LUC is a good marker for NRT2.1 expression
additional information
-
generation of several mutants devoid of NAR2.1, e.g. Atnar2.1-35S:NAR2.1-myc mutant, complementation by expression of wild-type NAR2.1, phenotypes, overview
additional information
-
mutation of the NRT1.5 nitrate transporter causes defective root-to-shoot nitrate transport, knockdown or knockout mutations of NRT1.5 reduces the amount of nitrate transported from the root to the shoot, Root and shoot nitrate contents of antisense NRT1.5 plants, overview. T-DNA-tagged mutants nrt1.5-1 and nrt1.5-2 are defective in long-distance transport of nitrate but not of sulfate or phosphate
-
additional information
nitrate uptake is restored in mutant chl1-5 plants by expression of the wild-type enzyme using the 35S::BcNRT1 construct
additional information
a single nucleotide polymorphism leads to an H433R substitution in subspecies indica. The indica type shows a significantly stronger nitrate uptake ability than the japonica type. Transgenic japonica lines harboring the indica allele translocate more nitrate to shoots and generally grow better than the japonica wild-type
additional information
-
construction of deletion mutants, NarK1 mutant grows almost normally, while NarK2 mutant shows strongly reduced generation times under nitrate-dependent anaerobic growth conditions, almost no growth of the NarK1/NarK2 double mutant, impaired growth can be restored by expression of the narK gene from Escherichia coli K-12
additional information
-
the NA3 mutant of strain PCC7942, constructed by deleting the nrtABCD genes from the nirA operon, is defective in the active transport of nitrate, an insertional mutant of NA3 is constructed for each of the four genes and analyzed for its nitrite transport ability, overview
additional information
-
the NA3 mutant of strain PCC7942, constructed by deleting the nrtABCD genes from the nirA operon, is defective in the active transport of nitrate, an insertional mutant of NA3 is constructed for each of the four genes and analyzed for its nitrite transport ability, overview
-
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a fusion protein between a 2-kb fragment of the ATNRT2.7 promoter and either the beta-glucuronidase or green fluorescent protein reporter gene stably introduced into the Arabidopsis thaliana ecotype Wassilewskija
-
ansient expression of the NRT1.8:enhanced green fluorescent protein fusion in onion epidermal cells and Arabidopsis protoplasts
-
expressed as GFP-fusion protein using Agrobacterium tumefaciens, correct localization
-
expressed in cells the nrtABCD deletion mutant NA3 of Synechococcus elongatus which restores the ability of the cell to grow on nitrate as sole nitrogen source
-
expressed in Escherichia coli
expressed in Xenopus laevis oocytes
expression of the pNRT2.1::LUC reporter gene construct in transgenic Arabidopsis thaliana plants, genotyping, overview
-
gene BcNRT1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Brassica campestris mesophyll protoplasts from pEarleyGate101 and pEarleyGate104 vectors or the cauliflower mosaic virus 35S promoter using the BcNRT1 promoter for analysis of subcellular localization by YFP fluorescence labeling, expression analysis by quantitative RT-PCR
gene Medtr5g093170.1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of NRT1.3 in Xenopus laevis oocytes
gene nrt2, DNA and amino acid sequence determination and analysis, detailed phylogenetic analysis
mutants and GFP-fusion protein, GFP-fusion protein is active and correctly localized
-
narK gene expressed in NarK2 and NarK1/NarK2 deletion mutants of Pseudomonas aeruginosa PAO1, narK gene expression is capable of restoring the anaerobic growth of Pseudomonas aeruginosa PAO1, although at reduced rates compared with the wild type
-
the nitrite transporting system is encoded by the four genes nrtA, -B, -C, and -D, organized in the CynABD operon genes, physical map of the cynABDS genomic region
-
transient expression of GFP-NRT1.9 fusion protein in Arabidopsis thaliana mesophyll protoplasts using the CMV 35S promoter
-
truncated NrtA protein lacking the N-terminal 81 amino acids, expressed in Escherichia coli cells as a histidine-tagged soluble protein
-
Xenopus laevis oocytes are injected with nuclease-free water or ATNRT2.7 mRNA, ATNRT2.7 mRNA-injected oocytes take up significantly more nitrate than water-injected controls
-
gene nrt2, DNA and amino acid sequence determination and analysis, detailed phylogenetic analysis
gene nrt2, DNA and amino acid sequence determination and analysis, detailed phylogenetic analysis
gene nrt2, DNA and amino acid sequence determination and analysis, detailed phylogenetic analysis
gene nrt2, DNA and amino acid sequence determination and analysis, detailed phylogenetic analysis
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
alternating wet and dry growth condition can induce the expression of NO3- and Mn transporters in grain filling organs which increases the accumulation of N and Mn in grain
enzyme expression is strongly influenced by plant nitrogen status at flowering during the first 300-400 degree-days after flowering
-
ex-pression of CHL1 (NRT1.1), NRT1.2, NRT2.1, NRT2.2, and/or NRT2.4 is regulated at the transcriptional level by nitrate, nitrite, ammonium, glutamine, N starvation, light, sucrose, diurnal rhythm, and/or pH
-
expression of NRT2/3 genes is induced in the roots by both low and high nitrogen levels
-
gene expression of Nrt2.1 and Nrt2.3 is strongly induced by low NO3- concentration
-
in response to salt stress (24 h treatment with 100 mM Na+), the expression of the root stele NO-3 transporter gene NPF2.3 is maintained
-
in roots, enzyme expression can be increased by 33% by supply of high nitrate (2.5 mM)
-
mRNA level of NRT1.5 is down-regulated by salt, drought as well as cadmium treatments
-
MtNRT1.3 is environmentally downregulated by the addition of NO3- to the roots
MtNRT1.3 is environmentally upregulated by the absence of NO3-, not as a result of a systemic signalling of plant N status
NRT1.8 is upregulated by nitrate. NRT1.8 is the only nitrate assimilatory pathway gene that is strongly upregulated by Cd2+ stress in roots
-
NRT2.1 transgenic Arabidopsis and Nicotiana reveal post-transcriptional repression of nitrate uptake in response to ammonium or dark treatments. In wild type, both the AtNRT2.1 mRNA level and the HATS are downregulated by prolonged dark. In the Arabidopsis 35S-NRT2.1 plant, the mRNA level remains unchanged in response to prolonged dark, but the HATS and NRT2.1 protein levels are reduced. cadmium treatment reduces the activity of NRT1.8 in the shoot
-
the expression of NRT1.5 is substantially induced by phosphate starvation
transporters NRT1.6, NRT1.7, NRT2.1, and NRT2.4 are inducible by nitrate. In wild type, expression of NRT1.8 is upregulated by cadmium
-
MtNRT1.3 is environmentally downregulated by the addition of NO3- to the roots
MtNRT1.3 is environmentally downregulated by the addition of NO3- to the roots
-
-
MtNRT1.3 is environmentally downregulated by the addition of NO3- to the roots
-
-
MtNRT1.3 is environmentally upregulated by the absence of NO3-, not as a result of a systemic signalling of plant N status
MtNRT1.3 is environmentally upregulated by the absence of NO3-, not as a result of a systemic signalling of plant N status
-
-
MtNRT1.3 is environmentally upregulated by the absence of NO3-, not as a result of a systemic signalling of plant N status
-
-
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
agriculture
analysis
-
transport assay in Xenopus laevis oocytes, directly measuring movement of six different gibberellic acids across oocyte membranes over short time. Membrane permeability of gibberellic acids in oocytes can be predicted based on number of oxygen atoms and several gibberellic acids do not diffuse over membranes regardless of changes in pH values. Small changes in internal cellular pH can result in strongly altered distribution of membrane permeable phytohormones
agriculture
changes in nitrogen sources can be crucial for root plasticity
agriculture
alternating wet and dry growth condition can induce the expression of NO3- and Mn transporters in grain filling organs which increases the accumulation of N and Mn in grain. NO3- uptake in NRT2.1 transgenic lines can improve Mn accumulation
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Omata, T.
Structure, function and regulation of the nitrate transport system of the cyanobacterium Synechococcus sp. PCC7942
Plant Cell Physiol.
36
207-213
1995
Synechococcus sp., Synechococcus sp. PCC 7942
brenda
Sakamoto, T.; Bryant, D.A.
Nitrate transport and not photoinhibition limits growth of the freshwater cyanobacterium Synechococcus species PCC 6301 at low temperature
Plant Physiol.
119
785-794
1999
Synechococcus sp., Synechococcus sp. PCC6301
brenda
Maeda, S.; Omata, T.
Substrate-binding lipoprotein of the cyanobacterium Synechococcus sp. strain PCC 7942 involved in the transport of nitrate and nitrite
J. Biol. Chem.
272
3036-3041
1997
Synechococcus sp., Synechococcus sp. PCC 7942
brenda
Kobayashi, M.; Rodriguez, R.; Lara, C.; Omata, T.
Involvement of the C-terminal domain of an ATP-binding subunit in the regulation of the ABC-type nitrate/nitrite transporter of the cyanobacterium Synechococcus sp. strain PCC 7942
J. Biol. Chem.
272
27197-27201
1997
Synechococcus sp., Synechococcus sp. PCC 7942
brenda
Omata, T.; Andriesse, X.; Hirano, A.
Identification and characterization of a gene cluster involved in nitrate transport in the cyanobacterium Synechococcus sp. PCC7942
Mol. Gen. Genet.
236
193-202
1993
Synechococcus sp., Synechococcus sp. PCC 7942
brenda
Wu, Q.; Stewart, V.
NasFED proteins mediate assimilatory nitrate and nitrite transport in Klebsiella oxytoca (pneumoniae) M5a1
J. Bacteriol.
180
1311-1322
1998
Klebsiella oxytoca, Klebsiella oxytoca M5a1
brenda
Rodriguez, R.; Kobayashi, M.; Omata, T.; Lara, C.
Independence of carbon and nitrogen control in the posttranslational regulation of nitrate transport in the cyanobacterium Synechococcus sp. strain PCC 7942
FEBS Lett.
432
207-212
1998
Synechococcus sp., Synechococcus sp. PCC 7942
brenda
Cava , C.F.; Berenguer , B.J.
Biochemical and regulatory properties of a respiratory island encoded by a conjugative plasmid in the extreme thermophile Thermus thermophilus
Biochem. Soc. Trans.
34
97-100
2006
Thermus thermophilus
brenda
Nagore, D.; Sanz, B.; Soria, J.; Llarena, M.; Llama, M.J.; Calvete, J.J.; Serra, J.L.
The nitrate/nitrite ABC transporter of Phormidium laminosum: phosphorylation state of NrtA is not involved in its substrate binding activity
Biochim. Biophys. Acta
1760
172-181
2006
Leptolyngbya laminosa
brenda
Sharma, V.; Noriega, C.E.; Rowe, J.J.
Involvement of NarK1 and NarK2 proteins in transport of nitrate and nitrite in the denitrifying bacterium Pseudomonas aeruginosa PAO1
Appl. Environ. Microbiol.
72
3802
2006
Pseudomonas aeruginosa, Escherichia coli
-
brenda
Aichi, M.; Yoshihara, S.; Yamashita, M.; Maeda, S.; Nagai, K.; Omata, T.
Characterization of the nitrate-nitrite transporter of the major facilitator superfamily (the nrtP gene product) from the cyanobacterium Nostoc punctiforme strain ATCC 29133
Biosci. Biotechnol. Biochem.
70
2682-2689
2006
Nostoc punctiforme
brenda
Navarro, F.J.; Machin, F.; Martin, Y.; Siverio, J.M.
Down-regulation of eukaryotic nitrate transporter by nitrogen-dependent ubiquitinylation
J. Biol. Chem.
281
13268-13274
2006
Ogataea angusta
brenda
Segonzac, C.; Boyer, J.C.; Ipotesi, E.; Szponarski, W.; Tillard, P.; Touraine, B.; Sommerer, N.; Rossignol, M.; Gibrat, R.
Nitrate efflux at the root plasma membrane: identification of an Arabidopsis excretion transporter
Plant Cell
19
3760-3777
2007
Arabidopsis thaliana (Q9M1E2)
brenda
Walch-Liu, P.; Forde, B.G.
Nitrate signalling mediated by the NRT1.1 nitrate transporter antagonises L-glutamate-induced changes in root architecture
Plant J.
54
820-828
2008
Arabidopsis thaliana
brenda
Remans, T.; Nacry, P.; Pervent, M.; Girin, T.; Tillard, P.; Lepetit, M.; Gojon, A.
A central role for the nitrate transporter NRT2.1 in the integrated morphological and physiological responses of the root system to nitrogen limitation in Arabidopsis
Plant Physiol.
140
909-921
2006
Arabidopsis thaliana
brenda
Leblanc, A.; Renault, H.; Lecourt, J.; Etienne, P.; Deleu, C.; Le Deunff, E.
Elongation changes of exploratory and root hair systems induced by aminocyclopropane carboxylic acid and aminoethoxyvinylglycine affect nitrate uptake and BnNrt2.1 and BnNrt1.1 transporter gene expression in oilseed rape
Plant Physiol.
146
1928-1940
2008
Brassica napus
brenda
Chopin, F.; Wirth, J.; Dorbe, M.F.; Lejay, L.; Krapp, A.; Gojon, A.; Daniel-Vedele, F.
The Arabidopsis nitrate transporter AtNRT2.1 is targeted to the root plasma membrane
Plant Physiol. Biochem.
45
630-635
2007
Arabidopsis thaliana
brenda
Camacho-Cristobal, J.J.; Gonzalez-Fontes, A.
Boron deficiency decreases plasmalemma H+-ATPase expression and nitrate uptake, and promotes ammonium assimilation into asparagine in tobacco roots
Planta
226
443-451
2007
Nicotiana tabacum
brenda
Maeda, S.; Omata, T.
Nitrite transport activity of the ABC-type cyanate transporter of the cyanobacterium Synechococcus elongatus
J. Bacteriol.
191
3265-3272
2009
Synechococcus elongatus, Synechococcus elongatus PCC 7942
brenda
Lin, S.H.; Kuo, H.F.; Canivenc, G.; Lin, C.S.; Lepetit, M.; Hsu, P.K.; Tillard, P.; Lin, H.L.; Wang, Y.Y.; Tsai, C.B.; Gojon, A.; Tsay, Y.F.
Mutation of the Arabidopsis NRT1.5 nitrate transporter causes defective root-to-shoot nitrate transport
Plant Cell
20
2514-2528
2008
Arabidopsis thaliana, Arabidopsis thaliana NRT1.5
brenda
Krouk, G.; Lacombe, B.; Bielach, A.; Perrine-Walker, F.; Malinska, K.; Mounier, E.; Hoyerova, K.; Tillard, P.; Leon, S.; Ljung, K.; Zazimalova, E.; Benkova, E.; Nacry, P.; Gojon, A.
Nitrate-regulated auxin transport by NRT1.1 defines a mechanism for nutrient sensing in plants
Dev. Cell
18
927-937
2010
Arabidopsis thaliana
brenda
Fan, S.C.; Lin, C.S.; Hsu, P.K.; Lin, S.H.; Tsay, Y.F.
The Arabidopsis nitrate transporter NRT1.7, expressed in phloem, is responsible for source-to-sink remobilization of nitrate
Plant Cell
21
2750-2761
2009
Arabidopsis thaliana
brenda
Li, J.Y.; Fu, Y.L.; Pike, S.M.; Bao, J.; Tian, W.; Zhang, Y.; Chen, C.Z.; Zhang, Y.; Li, H.M.; Huang, J.; Li, L.G.; Schroeder, J.I.; Gassmann, W.; Gong, J.M.
The Arabidopsis nitrate transporter NRT1.8 functions in nitrate removal from the xylem sap and mediates cadmium tolerance
Plant Cell
22
1633-1646
2010
Arabidopsis thaliana
brenda
Girin, T.; El-Kafafi, e.l.-.S.; Widiez, T.; Erban, A.; Hubberten, H.M.; Kopka, J.; Hoefgen, R.; Gojon, A.; Lepetit, M.
Identification of Arabidopsis mutants impaired in the systemic regulation of root nitrate uptake by the nitrogen status of the plant
Plant Physiol.
153
1250-1260
2010
Arabidopsis thaliana
brenda
Slot, J.; Hallstrom, K.; Matheny, P.; Hosaka, K.; Mueller, G.; Robertson, D.; Hibbett, D.
Phylogenetic, structural and functional diversification of nitrate transporters in three ecologically diverse clades of mushroom-forming fungi
Fungal Ecology
3
160-177
2010
Hebeloma helodes (C7SFH0), Hebeloma helodes (C7SFH1), Hebeloma radicosum (C7SFH7), Hebeloma velutipes (C7SFH8), Hebeloma sp. (C7SFI4), Hebeloma helodes JCS92306A (C7SFH0), Hebeloma helodes JCS92306A (C7SFH1), Hebeloma radicosum MYA2661 (C7SFH7), Hebeloma velutipes JCS91707A (C7SFH8), Hebeloma sp. PBM2674 (C7SFI4)
-
brenda
Morere-Le Paven, M.C.; Viau, L.; Hamon, A.; Vandecasteele, C.; Pellizzaro, A.; Bourdin, C.; Laffont, C.; Lapied, B.; Lepetit, M.; Frugier, F.; Legros, C.; Limami, A.M.
Characterization of a dual-affinity nitrate transporter MtNRT1.3 in the model legume Medicago truncatula
J. Exp. Bot.
62
5595-5605
2011
Medicago truncatula (D5LQ68), Medicago truncatula, Medicago truncatula DZA315.16 (D5LQ68), Medicago truncatula Jemalong-6 (D5LQ68)
brenda
Yang, X.; Sun, F.; Xiong, A.; Wang, F.; Kong, M.; Wang, Q.; Wang, J.; Dai, W.; Xia, X.; Hou, X.
BcNRT1, a plasma membrane-localized nitrate transporter from non-heading Chinese cabbage
Mol. Biol. Rep.
39
7997-8006
2012
Brassica rapa subsp. chinensis (H8Y6S4)
brenda
Wang, Y.Y.; Tsay, Y.F.
Arabidopsis nitrate transporter NRT1.9 is important in phloem nitrate transport
Plant Cell
23
1945-1957
2011
Arabidopsis thaliana
brenda
Bertoni, G.
A nitrate transporter for both roots and shoots
Plant Cell
24
1
2012
Arabidopsis thaliana
brenda
Kiba, T.; Feria-Bourrellier, A.B.; Lafouge, F.; Lezhneva, L.; Boutet-Mercey, S.; Orsel, M.; Brehaut, V.; Miller, A.; Daniel-Vedele, F.; Sakakibara, H.; Krapp, A.
The Arabidopsis nitrate transporter NRT2.4 plays a double role in roots and shoots of nitrogen-starved plants
Plant Cell
24
245-258
2012
Arabidopsis thaliana
brenda
Yong, Z.; Kotur, Z.; Glass, A.D.
Characterization of an intact two-component high-affinity nitrate transporter from Arabidopsis roots
Plant J.
63
739-748
2010
Arabidopsis thaliana
brenda
Chen, C.Z.; Lv, X.F.; Li, J.Y.; Yi, H.Y.; Gong, J.M.
Arabidopsis NRT1.5 is another essential component in regulation of nitrate reallocation and stress tolerance
Plant Physiol.
159
1582-1590
2012
Arabidopsis thaliana
brenda
Wang, Y.; Hsu, P.; Tsay, Y.
Uptake, allocation and signaling of nitrate
Trends Plant Sci.
17
458-467
2012
Arabidopsis thaliana, Chlamydomonas sp., Aspergillus sp.
brenda
Yan, H.; Huang, W.; Yan, C.; Gong, X.; Jiang, S.; Zhao, Y.; Wang, J.; Shi, Y.
Structure and mechanism of a nitrate transporter
Cell Rep.
3
716-723
2013
Escherichia coli (P37758)
brenda
Xia, X.; Fan, X.; Wei, J.; Feng, H.; Qu, H.; Xie, D.; Miller, A.J.; Xu, G.
Rice nitrate transporter OsNPF2.4 functions in low-affinity acquisition and long-distance transport
J. Exp. Bot.
66
317-331
2015
Oryza sativa
brenda
Chen, Z.; Ma, J.
Improving nitrogen use efficiency in rice through enhancing root nitrate uptake mediated by a nitrate transporter, NRT1.1B
J. Genet. Genomics
42
463-465
2015
Oryza sativa
brenda
Fan, X.; Feng, H.; Tan, Y.; Xu, Y.; Miao, Q.; Xu, G.
A putative 6 trans-membrane nitrate transporter OsNRT1.1b plays a key role in rice under low nitrogen
J. Integr. Plant Biol.
58
590-599
2015
Oryza sativa
brenda
Liu, X.; Cui, H.; Li, A.; Zhang, M.; Teng, Y.
The nitrate transporter NRT1.1 is involved in iron deficiency responses in Arabidopsis
J. Plant Nutr. Soil Sci.
178
601-608
2015
Arabidopsis thaliana
-
brenda
Parker, J.L.; Newstead, S.
Molecular basis of nitrate uptake by the plant nitrate transporter NRT1.1
Nature
507
68-72
2014
Arabidopsis thaliana (Q05085), Arabidopsis thaliana
brenda
Sun, J.; Bankston, J.R.; Payandeh, J.; Hinds, T.R.; Zagotta, W.N.; Zheng, N.
Crystal structure of the plant dual-affinity nitrate transporter NRT1.1
Nature
507
73-77
2014
Arabidopsis thaliana (Q05085)
brenda
Charrier, A.; Berard, J.B.; Bougaran, G.; Carrier, G.; Lukomska, E.; Schreiber, N.; Fournier, F.; Charrier, A.F.; Rouxel, C.; Garnier, M.; Cadoret, J.P.; Saint-Jean, B.
High-affinity nitrate/nitrite transporter genes (Nrt2) in Tisochrysis lutea: identification and expression analyses reveal some interesting specificities of Haptophyta microalgae
Physiol. Plant.
154
572-590
2015
Tisochrysis lutea
brenda
Lezhneva, L.; Kiba, T.; Feria-Bourrellier, A.B.; Lafouge, F.; Boutet-Mercey, S.; Zoufan, P.; Sakakibara, H.; Daniel-Vedele, F.; Krapp, A.
The Arabidopsis nitrate transporter NRT2.5 plays a role in nitrate acquisition and remobilization in nitrogen-starved plants
Plant J.
80
230-241
2014
Arabidopsis thaliana
brenda
Taochy, C.; Gaillard, I.; Ipotesi, E.; Oomen, R.; Leonhardt, N.; Zimmermann, S.; Peltier, J.B.; Szponarski, W.; Simonneau, T.; Sentenac, H.; Gibrat, R.; Boyer, J.C.
The Arabidopsis root stele transporter NPF2.3 contributes to nitrate translocation to shoots under salt stress
Plant J.
83
466-479
2015
Arabidopsis thaliana
brenda
Bagchi, R.; Salehin, M.; Adeyemo, O.S.; Salazar, C.; Shulaev, V.; Sherrier, D.J.; Dickstein, R.
Functional assessment of the Medicago truncatula NIP/LATD protein demonstrates that it is a high-affinity nitrate transporter
Plant Physiol.
160
906-916
2012
Medicago truncatula
brenda
Pellizzaro, A.; Clochard, T.; Cukier, C.; Bourdin, C.; Juchaux, M.; Montrichard, F.; Thany, S.; Raymond, V.; Planchet, E.; Limami, A.M.; Morere-Le Paven, M.C.
The nitrate transporter MtNPF6.8 (MtNRT1.3) transports abscisic acid and mediates nitrate regulation of primary root growth in Medicago truncatula
Plant Physiol.
166
2152-2165
2014
Medicago truncatula
brenda
Taulemesse, F.; Le Gouis, J.; Gouache, D.; Gibon, Y.; Allard, V.
Post-flowering nitrate uptake in wheat is controlled by N status at flowering, with a putative major role of root nitrate transporter NRT2.1
PLoS ONE
10
e0120291
2015
Triticum aestivum
brenda
Giffin, M.M.; Raab, R.W.; Morganstern, M.; Sohaskey, C.D.
Mutational analysis of the respiratory nitrate transporter NarK2 of Mycobacterium tuberculosis
PLoS ONE
7
e45459
2012
Mycobacterium tuberculosis (P9WJY7), Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv (P9WJY7)
brenda
Li, G.; Tillard, P.; Gojon, A.; Maurel, C.
Dual regulation of root hydraulic conductivity and plasma membrane aquaporins by plant nitrate accumulation and high-affinity nitrate transporter NRT2.1
Plant Cell Physiol.
57
733-742
2016
Arabidopsis thaliana
brenda
Zheng, Y.; Drechsler, N.; Rausch, C.; Kunze, R.
The Arabidopsis nitrate transporter NPF7.3/NRT1.5 is involved in lateral root development under potassium deprivation
Plant Signal. Behav.
11
e1176819
2016
Arabidopsis thaliana
brenda
Cui, Y.N.; Li, X.T.; Yuan, J.Z.; Wang, F.Z.; Wang, S.M.; Ma, Q.
Nitrate transporter NPF7.3/NRT1.5 plays an essential role in regulating phosphate deficiency responses in Arabidopsis
Biochem. Biophys. Res. Commun.
508
314-319
2019
Arabidopsis thaliana (Q9LQL2)
brenda
Wulff, N.; Ernst, H.A.; Joergensen, M.E.; Lambertz, S.; Maierhofer, T.; Belew, Z.M.; Crocoll, C.; Motawia, M.S.; Geiger, D.; Joergensen, F.S.; Mirza, O.; Nour-Eldin, H.H.
An optimized screen reduces the number of GA transporters and provides insights into nitrate transporter 1/peptide transporter family substrate determinants
Front. Plant Sci.
10
1106
2019
Arabidopsis thaliana
brenda
van Gelderen, K.; Kang, C.; Li, P.; Pierik, R.
Regulation of lateral root development by shoot-sensed far-red light via HY5 is nitrate-dependent and involves the NRT2.1 nitrate transporter
Front. Plant Sci.
12
660870
2021
Arabidopsis thaliana (O82811)
brenda
Zang, L.; Tarkowski, L.P.; Morere-Le Paven, M.C.; Zivy, M.; Balliau, T.; Clochard, T.; Bahut, M.; Balzergue, S.; Pelletier, S.; Landes, C.; Limami, A.M.; Montrichard, F.
The nitrate transporter MtNPF6.8 is a master sensor of nitrate signal in the primary root tip of Medicago truncatula
Front. Plant Sci.
13
832246
2022
Medicago truncatula
brenda
Luo, B.; Chen, J.; Zhu, L.; Liu, S.; Li, B.; Lu, H.; Ye, G.; Xu, G.; Fan, X.
Overexpression of a high-affinity nitrate transporter OsNRT2.1 increases yield and manganese accumulation in rice under alternating wet and dry condition
Front. Plant Sci.
9
1192
2018
Oryza sativa Japonica Group (P0DKG9)
brenda
Rubio, L.; Diaz-Garcia, J.; Amorim-Silva, V.; Macho, A.P.; Botella, M.A.; Fernandez, J.A.
Molecular characterization of ZosmaNRT2, the putative sodium dependent high-affinity nitrate transporter of Zostera marina L.
Int. J. Mol. Sci.
20
3650
2019
Zostera marina (A0A0K9NQH5)
brenda
Wei, J.; Zheng, Y.; Feng, H.; Qu, H.; Fan, X.; Yamaji, N.; Ma, J.F.; Xu, G.
OsNRT2.4 encodes a dual-affinity nitrate transporter and functions in nitrate-regulated root growth and nitrate distribution in rice
J. Exp. Bot.
69
1095-1107
2018
Oryza sativa Japonica Group (A2ZU80)
brenda
Zhu, J.; Fang, X.Z.; Dai, Y.J.; Zhu, Y.X.; Chen, H.S.; Lin, X.Y.; Jin, C.W.
Nitrate transporter 1.1 alleviates lead toxicity in Arabidopsis by preventing rhizosphere acidification
J. Exp. Bot.
70
6363-6374
2019
Arabidopsis thaliana (Q05085)
brenda
Wang, H.; Wan, Y.; Buchner, P.; King, R.; Ma, H.; Hawkesford, M.J.
Phylogeny and gene expression of the complete NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER FAMILY in Triticum aestivum
J. Exp. Bot.
71
4531-4546
2020
Triticum aestivum
brenda
Zou, X.; Liu, M.Y.; Wu, W.H.; Wang, Y.
Phosphorylation at Ser28 stabilizes the Arabidopsis nitrate transporter NRT2.1 in response to nitrate limitation
J. Integr. Plant Biol.
62
865-876
2020
Arabidopsis thaliana (O82811)
brenda
Xiao, C.; Sun, D.; Liu, B.; Fang, X.; Li, P.; Jiang, Y.; He, M.; Li, J.; Luan, S.; He, K.
Nitrate transporter NRT1.1 and anion channel SLAH3 form a functional unit to regulate nitrate-dependent alleviation of ammonium toxicity
J. Integr. Plant Biol.
64
942-957
2022
Arabidopsis thaliana (Q05085)
brenda
Maeda, S.I.; Aoba, R.; Nishino, Y.; Omata, T.
A novel bacterial nitrate transporter composed of small transmembrane proteins
Plant Cell Physiol.
60
2180-2192
2019
Synechococcus elongatus, Marinomonas mediterranea, Picosynechococcus sp. PCC 7002, Synechococcus elongatus PCC 7942
brenda
Guan, Y.; Liu, D.F.; Qiu, J.; Liu, Z.J.; He, Y.N.; Fang, Z.J.; Huang, X.H.; Gong, J.M.
The nitrate transporter OsNPF7.9 mediates nitrate allocation and the divergent nitrate use efficiency between indica and japonica rice
Plant Physiol.
189
215-229
2022
Oryza sativa Japonica Group (Q0DYI7)
brenda
Guo, B.; Li, Y.; Wang, S.; Li, D.; Lv, C.; Xu, R.
Characterization of the nitrate transporter gene family and functional identification of HvNRT2.1 in barley (Hordeum vulgare L.)
PLoS ONE
15
e0232056
2020
Hordeum vulgare
brenda
Swapnil, P.; Meena, M.; Rai, A.K.
Molecular interaction of nitrate transporter proteins with recombinant glycinebetaine results in efficient nitrate uptake in the cyanobacterium Anabaena PCC 7120
PLoS ONE
16
e0257870
2021
Nostoc sp. PCC 7120 = FACHB-418 (Q8YRV9 and Q8YZ76 and Q8YZ75)
brenda
EXTERNAL LINKS (specific for EC-Number 7.3.2.4)
Transporter Classification Database (TCDB): 3.A.1.16.1
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