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Literature summary for 7.3.2.4 extracted from
- Arabidopsis nitrate transporter NRT1.9 is important in phloem nitrate transport (2011), Plant Cell, 23, 1945-1957.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| transient expression of GFP-NRT1.9 fusion protein in Arabidopsis thaliana mesophyll protoplasts using the CMV 35S promoter | Arabidopsis thaliana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | uptake kinetics of recombinant NRT1.9 in NRT1.9-injected oocytes, pH 5.5 | Arabidopsis thaliana |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| plasma membrane | - |
Arabidopsis thaliana | 5886 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| companion cell | NRT1.9 is a transporter expressed in the companion cells of root phloem | Arabidopsis thaliana | - |
| additional information | quantitative RT-PCR NRT1.9 expression analysis | Arabidopsis thaliana | - |
| phloem | - |
Arabidopsis thaliana | - |
| root | NRT1.9 is mainly expressed in roots, in phloem | Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | NRT1.9 is a low-affinity nitrate transporter | Arabidopsis thaliana | ? | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| nitrate transporter | - |
Arabidopsis thaliana |
| NRT1.9 | - |
Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
recombinant NRT1.9 in frog oocytes, transport assay at | Arabidopsis thaliana |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme is a member of the NRT1 family | Arabidopsis thaliana |
| malfunction | in nrt1.9 mutants, nitrate content in root phloem exudates is decreased, and downward nitrate transport is reduced. Under high nitrate conditions, the nrt1.9 mutant shows enhanced root-to-shoot nitrate transport and plant growth, phenotypes, overview | Arabidopsis thaliana |
| physiological function | NRT1.9 facilitates loading of nitrate into the root phloem and enhance downward nitrate transport in roots, expression of NRT1.9 in root companion cells | Arabidopsis thaliana |
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Release 2023.1 (January 2023)
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