Literature summary for 7.3.2.4 extracted from

Li, J.Y.; Fu, Y.L.; Pike, S.M.; Bao, J.; Tian, W.; Zhang, Y.; Chen, C.Z.; Zhang, Y.; Li, H.M.; Huang, J.; Li, L.G.; Schroeder, J.I.; Gassmann, W.; Gong, J.M.
The Arabidopsis nitrate transporter NRT1.8 functions in nitrate removal from the xylem sap and mediates cadmium tolerance (2010), Plant Cell, 22, 1633-1646.

Search for more literature for 7.3.2.4

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	the nrt1.8-1 mutant shows a nitrate-dependent Cd2+-sensitive phenotype, and nitrate transport by NRT1.8 is pH-dependent	Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment)	Organism
ansient expression of the NRT1.8:enhanced green fluorescent protein fusion in onion epidermal cells and Arabidopsis protoplasts	Arabidopsis thaliana

Inhibitors

Inhibitors	Comment	Organism	Structure
Cd2+	the nrt1.8-1 mutant shows a nitrate-dependent Cd2+-sensitive phenotype	Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
plasma membrane	subcellular localization study, overview	Arabidopsis thaliana	5886	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cd2+	the nrt1.8-1 mutant shows a nitrate-dependent Cd2+-sensitive phenotype, while wild-type NRT1.8 is upregulated by Cd2+ stress	Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O + nitrate/out	Arabidopsis thaliana	NRT1.8 can transport nitrate and regulates nitrate removal from xylem. Nitrate distribution under Cd2+ stress is altered by NRT1.8	ADP + phosphate + nitrate/in	-	?
additional information	Arabidopsis thaliana	NRT1.8, injected in Xenopus laevis oocytes, mediates low-affinity nitrate uptake	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Arabidopsis thaliana	-
parenchyma	histochemical analysis shows that NRT1.8 is expressed predominantly in xylem parenchyma cells within the vasculature	Arabidopsis thaliana	-
xylem	histochemical analysis shows that NRT1.8 is expressed predominantly in xylem parenchyma cells within the vasculature	Arabidopsis thaliana	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + nitrate/out	-	Arabidopsis thaliana	ADP + phosphate + nitrate/in	-	?
ATP + H2O + nitrate/out	NRT1.8 can transport nitrate and regulates nitrate removal from xylem. Nitrate distribution under Cd2+ stress is altered by NRT1.8	Arabidopsis thaliana	ADP + phosphate + nitrate/in	-	?
additional information	NRT1.8, injected in Xenopus laevis oocytes, mediates low-affinity nitrate uptake	Arabidopsis thaliana	?	-	?

Synonyms

Synonyms	Comment	Organism
More	NRT1.8 is a member of the nitrate transporter, NRT1, family	Arabidopsis thaliana
nitrate transporter	-	Arabidopsis thaliana
NRT1.8	-	Arabidopsis thaliana

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
additional information	-	nitrate transport by NRT1.8 is pH-dependent	Arabidopsis thaliana

Expression

Organism	Comment	Expression
Arabidopsis thaliana	NRT1.8 is upregulated by nitrate. NRT1.8 is the only nitrate assimilatory pathway gene that is strongly upregulated by Cd2+ stress in roots	up

General Information

General Information	Comment	Organism
metabolism	NRT1.8 is the only nitrate assimilatory pathway gene that is strongly upregulated by Cd2+ stress in roots. NRT1.8-regulated nitrate distribution plays an important role in Cd2+ tolerance	Arabidopsis thaliana
additional information	functional disruption of NRT1.8 significantly increased the nitrate concentration in xylem sap	Arabidopsis thaliana
physiological function	nitrate transporter NRT1.8 functions in nitrate removal from the xylem sap and mediates cadmium tolerance	Arabidopsis thaliana

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Release 2023.1 (January 2023)