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IUBMB Comments Highly specific; does not hydrolyse other dioxopiperazines, glycylglycine, proteins or barbiturates.
Synonyms
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5,10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase
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cyclo(Gly-Gly) hydrolase
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hydrolase, cyclo(glycylglycine)
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2,5-dioxopiperazine + H2O = glycylglycine
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hydrolysis of cyclic amides
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2,5-dioxopiperazine amidohydrolase
Highly specific; does not hydrolyse other dioxopiperazines, glycylglycine, proteins or barbiturates.
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2,5-dioxopiperazine + H2O
glycylglycine
formyltetrahydrofolate + H2O
methylenetetrahydrofolate + ?
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Substrates: -
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2,5-dioxopiperazine + H2O
glycylglycine
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Substrates: highly specific, does not hydrolyze other piperazines
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2,5-dioxopiperazine + H2O
glycylglycine
Bacillus sp. (in: firmicutes) No. 106
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Substrates: highly specific, does not hydrolyze other piperazines
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additional information
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5 - 10
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pH 5: about 20% of maximal activity, pH 10: about 50% of maximal activity
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30 - 70
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30°C: about 50% of maximal activity, 70°C: about 70% of maximal activity
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Bacillus sp. (in: firmicutes) No. 106
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strain DSM 1728
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Bacillus sp. (in: firmicutes) No. 106
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brenda
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Bacillus sp. (in: firmicutes) No. 106
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Highest Expressing Human Cell Lines
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Cell Line Links
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dimer
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x-ray crystallography
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in the presence of NADP+, at 22°C, using polyethylene glycol 4000 as a precipitant, sitting drop vapour diffusion method
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5.5 - 9
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30°C, 3 h, stable
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50
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pH 7.0, 15 min, stable below
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His-Trap affinity column chromatography and Superdex-S200 gel filtration
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expressed in Escherichia coli strain BL21 (DE3)
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Muro, T.; Tominaga, Y.; Okada, S.
Purification and some properties of cyclo(Gly-Gly) hydrolase from a strain of Bacillus sp. No. 106
Agric. Biol. Chem.
49
1567-1572
1985
Bacillus sp. (in: firmicutes), Bacillus sp. (in: firmicutes) No. 106
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Kim, J.H.; Sung, M.W.; Lee, E.H.; Nam, K.H.; Hwang, K.Y.
Crystallization and preliminary X-ray diffraction analysis of 5,10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase from Thermoplasma acidophilum DSM 1728
J. Microbiol. Biotechnol.
18
283-286
2008
Thermoplasma acidophilum
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