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Highly specific; does not hydrolyse other dioxopiperazines, glycylglycine, proteins or barbiturates.
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The expected taxonomic range for this enzyme is: Bacteria, Archaea
5,10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase, cyclo(Gly-Gly) hydrolase, hydrolase, cyclo(glycylglycine), MTHFDC, more
2,5-dioxopiperazine + H2O = glycylglycine
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