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Literature summary for 3.5.2.13 extracted from

  • Kim, J.H.; Sung, M.W.; Lee, E.H.; Nam, K.H.; Hwang, K.Y.
    Crystallization and preliminary X-ray diffraction analysis of 5,10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase from Thermoplasma acidophilum DSM 1728 (2008), J. Microbiol. Biotechnol., 18, 283-286.
    View publication on PubMed
Search for more literature for 3.5.2.13

Activating Compound

Activating Compound Comment Organism Structure
NAD+
-
 Thermoplasma acidophilum
NADP+
-
 Thermoplasma acidophilum

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21 (DE3) Thermoplasma acidophilum

Crystallization (Commentary)

Crystallization (Comment) Organism
in the presence of NADP+, at 22°C, using polyethylene glycol 4000 as a precipitant, sitting drop vapour diffusion method Thermoplasma acidophilum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30600
-
 SDS-PAGE Thermoplasma acidophilum

Organism

Organism UniProt Comment Textmining
Thermoplasma acidophilum
-
 strain DSM 1728
-

Purification (Commentary)

Purification (Comment) Organism
His-Trap affinity column chromatography and Superdex-S200 gel filtration Thermoplasma acidophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formyltetrahydrofolate + H2O
-
 Thermoplasma acidophilum methylenetetrahydrofolate + ?
-
 ?

Subunits

Subunits Comment Organism
dimer x-ray crystallography Thermoplasma acidophilum

Synonyms

Synonyms Comment Organism
5,10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase
-
 Thermoplasma acidophilum
MTHFDC
-
 Thermoplasma acidophilum