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Literature summary for 3.5.2.13 extracted from

  • Muro, T.; Tominaga, Y.; Okada, S.
    Purification and some properties of cyclo(Gly-Gly) hydrolase from a strain of Bacillus sp. No. 106 (1985), Agric. Biol. Chem., 49, 1567-1572.
No PubMed abstract available
Search for more literature for 3.5.2.13

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Bacillus sp. (in: Bacteria)
monoiodoacetate
-
 Bacillus sp. (in: Bacteria)
Ni2+
-
 Bacillus sp. (in: Bacteria)
PCMB
-
 Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
11.1
-
 cyclo(Gly-Gly)
-
 Bacillus sp. (in: Bacteria)

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Bacillus sp. (in: Bacteria)
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Bacillus sp. (in: Bacteria)
Mg2+ activates Bacillus sp. (in: Bacteria)
Na+ activates Bacillus sp. (in: Bacteria)
Sr2+ activates Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-
Bacillus sp. (in: Bacteria) No. 106
-
-
-

Purification (Commentary)

Purification (Comment) Organism
strain No. 106 Bacillus sp. (in: Bacteria)

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Bacillus sp. (in: Bacteria)
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,5-dioxopiperazine + H2O highly specific, does not hydrolyze other piperazines Bacillus sp. (in: Bacteria) glycylglycine
-
 ?
2,5-dioxopiperazine + H2O highly specific, does not hydrolyze other piperazines Bacillus sp. (in: Bacteria) No. 106 glycylglycine
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Bacillus sp. (in: Bacteria)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 30°C: about 50% of maximal activity, 70°C: about 70% of maximal activity Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 pH 7.0, 15 min, stable below Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
 Bacillus sp. (in: Bacteria)

pH Range

pH Minimum pH Maximum Comment Organism
5 10 pH 5: about 20% of maximal activity, pH 10: about 50% of maximal activity Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 9 30°C, 3 h, stable Bacillus sp. (in: Bacteria)