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Information on EC 3.5.1.22 - pantothenase
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3.5.1.22 pantothenaseSpecify your search results
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- 3.5.1.22
- fluorescens
- oxaloacetate
-
phenylmethanesulphonyl
- fluoride
- beta-alanine
- nutrition
The enzyme appears in viruses and cellular organisms
Synonyms
pantothenate hydrolase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
pantothenate hydrolase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
(R)-pantothenate + H2O = (R)-pantoate + beta-alanine
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of peptide bond
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
(R)-pantothenate amidohydrolase
-
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CAS REGISTRY NUMBER
COMMENTARY
9076-90-8
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
pantothenate + H2O
2,4-dihydroxy-3,3-dimethylbutyric acid + beta-alanine
-
-
-
-
?
pantoyl-gamma-aminobutyrate + H2O
4-aminobutyrate + D-pantoate
-
-
-
-
?
pantothenic acid + H2O
?
-
-
-
-
?
pantothenic acid + H2O
?
-
sole source of carbon and nitrogen
-
-
?
pantothenic acid + H2O
beta-alanine + D-pantoic acid
-
PS-21
-
ir
pantothenic acid + H2O
beta-alanine + D-pantoic acid
-
PS-21
-
ir
pantothenic acid + H2O
beta-alanine + D-pantoyl lactone
-
-
-
-
?
pantothenic acid + H2O
beta-alanine + D-pantoyl lactone
-
-
-
-
r
pantothenic acid + H2O
beta-alanine + D-pantoyl lactone
-
-
-
-
r
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
pantothenic acid + H2O
?
-
-
-
-
?
pantothenic acid + H2O
?
-
sole source of carbon and nitrogen
-
-
?
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
oxalate
-
same ligands inhibits enzyme act as effective protectors against thermal inactivation
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
3.2
pantothenic acid
-
PS-21, pH 8.1, 50 mM diethanolamine/H2SO4, Km-value pH independent
15
pantothenic acid
-
dependent on pH, phosphate buffer, pH 7.0, 20°C
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
NCIB12017, earlier Pseudomonas fluorescens UK-1, isolated from sea water
-
-
brenda
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
dimer
tetramer
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
28 - 37
-
partial inactivation, reactivation with oxalate, oxaloacetate or pyruvate to 10-20% of original activity, 30 min at 35°, inactivation
28 - 40
30 - 35
-
above 30°C rapidly inactivated, at 35°C inactivation rate 7.2%/min
28 - 40
-
thermal inactivation, reactivation when temperature is lowered again
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C 20 mM potassium phosphate pH 7, enzyme is made accessible to substrates by freezing and thawing four times, decrease of activity 20% per round of freezing and thawing, frozen enzyme retains activity for at least 2 years
-
-20°C enzyme is stable frozen, but loses activity in solution at 0°C in a few days
-
-20°C, 5 mM K2SO4, repeated cycles of freezing and thawing inactivate the enzyme
-
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
nutrition
-
vitamin determination, extremely sensitive assay of pantothenic acid in various dietary sources, as low as 5 nM concentration
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kalvero Airas, R.
Pantothenase-based assay of pantothenic acid
Methods Enzymol.
122
33-35
1986
Pseudomonas fluorescens
brenda
Kalvero Airas, R.
Pantothenase-based assay of pantothenic acid
Anal. Biochem.
134
122-125
1983
Pseudomonas fluorescens
brenda
Kalvero Airas, R.
Pantothenase from Pseudomonas fluorescens
Methods Enzymol.
62
267-275
1979
Pseudomonas fluorescens
brenda
Solberg, O.; Hegma, I.K.
Microbiological assay of pantothenic acid
Methods Enzymol.
62
201-205
1979
Lactiplantibacillus plantarum, Pediococcus acidilactici
brenda
Kalvero Airas, R.
Kinetic study on the reaction mechanism of pantothenase:Existence of an acyl-enzyme intermediate and role of general acid catalysis
Biochemistry
17
4932-4939
1978
Pseudomonas fluorescens
brenda
Kalvero Airas, R.
On the partial reactivation of inactivated pantothenase from Pseudomonas fluorescens
Biochim. Biophys. Acta
452
201-208
1976
Pseudomonas fluorescens
brenda
Kalvero Airas, R.
Thermal inactivation of pantothenase from Pseudomonas fluorescens
Biochim. Biophys. Acta
452
193-200
1976
Pseudomonas fluorescens
brenda
Kalvero Airas, R.
Kinetic studies on pantothenase of Pseudomonas fluorescens
Biochem. J.
157
415-421
1976
Pseudomonas fluorescens, Pseudomonas fluorescens P-2
brenda
Kalvero Airas, R.; Hietanen, E.A.; Nurmikko, V.T.
Purification and properties of pantothenase from Pseudomonas fluorescens
Biochem. J.
157
409-413
1976
Pseudomonas fluorescens, Pseudomonas fluorescens P-2
brenda
Kalvero Airas, R.
Thermal inactivation and reactivation of an enzyme in vivo
Biochem. J.
130
111-119
1972
Pseudomonas fluorescens
brenda
Nurmikko, V.; Salo, E.; Hakkola, H.; Maekinen, K.; Snell, E.E.
The bacterial degradation of pantothenic acid. II. pantothenate hydrolase
Biochemistry
5
399-403
1966
Pseudomonas fluorescens, Pseudomonas fluorescens P-2
brenda
Kalvero Airas, R.
Pantothenases from pseudomonads produce either pantoyl lactone or pantoic acid
Biochem. J.
250
447-451
1988
Pseudomonas fluorescens, Pseudomonas fluorescens PS-21
brenda
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