Inhibitors | Comment | Organism | Structure |
---|---|---|---|
oxalate | - |
Pseudomonas fluorescens | |
oxaloacetate | noncompetitive inhibition, dependent on pH | Pseudomonas fluorescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.6 | - |
pantothenic acid | pH-independent theoretical Km | Pseudomonas fluorescens | |
15 | - |
pantothenic acid | dependent on pH, phosphate buffer, pH 7.0, 20°C | Pseudomonas fluorescens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Pseudomonas fluorescens | 5622 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
2 * 50000, SDS-PAGE | Pseudomonas fluorescens |
97400 | - |
gel filtration | Pseudomonas fluorescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pantothenic acid + H2O | Pseudomonas fluorescens | sole source of carbon and nitrogen | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas fluorescens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
14.8 | - |
- |
Pseudomonas fluorescens |
Storage Stability | Organism |
---|---|
-20°C 20 mM potassium phosphate pH 7, enzyme is made accessible to substrates by freezing and thawing four times, decrease of activity 20% per round of freezing and thawing, frozen enzyme retains activity for at least 2 years | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pantothenate + H2O | - |
Pseudomonas fluorescens | 2,4-dihydroxy-3,3-dimethylbutyric acid + beta-alanine | - |
? | |
pantothenic acid + H2O | - |
Pseudomonas fluorescens | beta-alanine + D-pantoyl lactone + H2O | - |
r | |
pantothenic acid + H2O | - |
Pseudomonas fluorescens | beta-alanine + D-pantoic acid | - |
ir | |
pantothenic acid + H2O | sole source of carbon and nitrogen | Pseudomonas fluorescens | ? | - |
? | |
pantoyl-gamma-aminobutyrate + H2O | - |
Pseudomonas fluorescens | 4-aminobutyrate + D-pantoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 50000, SDS-PAGE | Pseudomonas fluorescens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
- |
Pseudomonas fluorescens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 35 | above 30°C rapidly inactivated, at 35°C inactivation rate 7.2%/min | Pseudomonas fluorescens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9.5 | - |
Pseudomonas fluorescens |