This membrane-bound enzyme is located in the plastids and requires Mg2+ for activity. The reaction forms the lavandulyl sidechain of sophoraflavanone G by transferring a prenyl group to the 2'' position of another prenyl group attached at position 8 of leachianone G. The enzyme is specific for prenyl diphosphate as the prenyl donor, as it cannot be replaced by isopentenyl diphosphate or geranyl diphosphate. Euchrenone a7 (a 5-deoxy derivative of leachianone G) and kenusanone I (a 7-methoxy derivative of leachianone G) can also act as substrates, but more slowly. Along with EC 1.14.14.142 (8-dimethylallylnaringenin 2'-hydroxylase) and EC 2.5.1.70 (naringenin 8-dimethylallyltransferase), this enzyme forms part of the sophoraflavanone-G-biosynthesis pathway.
This membrane-bound enzyme is located in the plastids and requires Mg2+ for activity. The reaction forms the lavandulyl sidechain of sophoraflavanone G by transferring a prenyl group to the 2'' position of another prenyl group attached at position 8 of leachianone G. The enzyme is specific for prenyl diphosphate as the prenyl donor, as it cannot be replaced by isopentenyl diphosphate or geranyl diphosphate. Euchrenone a7 (a 5-deoxy derivative of leachianone G) and kenusanone I (a 7-methoxy derivative of leachianone G) can also act as substrates, but more slowly. Along with EC 1.14.14.142 (8-dimethylallylnaringenin 2'-hydroxylase) and EC 2.5.1.70 (naringenin 8-dimethylallyltransferase), this enzyme forms part of the sophoraflavanone-G-biosynthesis pathway.
no activity with isopentenyl diphosphate and geranyl diphosphate as prenyl donors, and with kenusanone I, 8-dimethylallylnaringenin, and isovabachin as acceptor substrates, overview
the enzyme transfers a dimethylallyl group to the 2'' position of another dimethylallyl group attached at position 8 of leachianone G to form sophoraflavanone G, a branched monoterpenoid-conjugated flavanone characteristic to this plant
the enzyme is a prenyl side-chain elongation enzyme, the enzyme transfers a dimethylallyl group to the 2'' position of another dimethylallyl group attached at position 8 of leachianone G to form sophoraflavanone G, a branched monoterpenoid-conjugated flavanone, the enzyme is absolutely specific for dimethylallyl diphosphate as prenyl donor and highly specific for leachianone G, the 2'-hydroxy function in leachianone G is indispensable to the activity
the enzyme transfers a dimethylallyl group to the 2'' position of another dimethylallyl group attached at position 8 of leachianone G to form sophoraflavanone G, a branched monoterpenoid-conjugated flavanone characteristic to this plant
Characterization of leachianone G 2''-dimethylallyltransferase, a novel prenyl side-chain elongation enzyme for the formation of the lavandulyl group of sophoraflavanone G in Sophora flavescens Ait. cell suspension cultures
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2.5.1.71 leachianone-G 2''-dimethylallyltransferase
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