Literature summary for 2.5.1.71 extracted from

  • Zhao, P.; Inoue, K.; Kouno, I.; Yamamoto, H.
    Characterization of leachianone G 2''-dimethylallyltransferase, a novel prenyl side-chain elongation enzyme for the formation of the lavandulyl group of sophoraflavanone G in Sophora flavescens Ait. cell suspension cultures (2003), Plant Physiol., 133, 1306-1313.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0023
-
leachianone G pH 8.8, 30°C Sophora flavescens
0.059
-
dimethylallyl diphosphate pH 8.8, 30°C Sophora flavescens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane bound, determination of enzyme distibution in membranes Sophora flavescens
-
-
chloroplast
-
Sophora flavescens
-
-
additional information subcellular localization of the enzymes involved in the biosynthetic route from naringenin to sophoraflavanone G, overview Sophora flavescens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ 10% of the activity with Mg2+ Sophora flavescens
Mn2+ 15% of the activity with Mg2+ Sophora flavescens
Ca2+ 25% of the activity with Mg2+ Sophora flavescens
Cu2+ 4% of the activity with Mg2+ Sophora flavescens
Co2+ 5% of the activity with Mg2+ Sophora flavescens
Fe2+ 5% of the activity with Mg2+ Sophora flavescens
Ni2+ 5% of the activity with Mg2+ Sophora flavescens
Mg2+ required, optimal at 10 mM, other divalent cations can only partially substitute for Mg2+, overview Sophora flavescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dimethylallyl diphosphate + leachianone G Sophora flavescens the enzyme transfers a dimethylallyl group to the 2'' position of another dimethylallyl group attached at position 8 of leachianone G to form sophoraflavanone G, a branched monoterpenoid-conjugated flavanone characteristic to this plant diphosphate + sophoraflavanone G
-
?

Organism

Organism UniProt Comment Textmining
Sophora flavescens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme partially by microsome preparation Sophora flavescens

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
Sophora flavescens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.06
-
microsomes Sophora flavescens
0.00014
-
crude cell extract Sophora flavescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with isopentenyl diphosphate and geranyl diphosphate as prenyl donors, and with kenusanone I, 8-dimethylallylnaringenin, and isovabachin as acceptor substrates, overview Sophora flavescens ?
-
?
dimethylallyl diphosphate + leachianone G the enzyme transfers a dimethylallyl group to the 2'' position of another dimethylallyl group attached at position 8 of leachianone G to form sophoraflavanone G, a branched monoterpenoid-conjugated flavanone characteristic to this plant Sophora flavescens diphosphate + sophoraflavanone G
-
?
dimethylallyl diphosphate + leachianone G the enzyme is a prenyl side-chain elongation enzyme, the enzyme transfers a dimethylallyl group to the 2'' position of another dimethylallyl group attached at position 8 of leachianone G to form sophoraflavanone G, a branched monoterpenoid-conjugated flavanone, the enzyme is absolutely specific for dimethylallyl diphosphate as prenyl donor and highly specific for leachianone G, the 2'-hydroxy function in leachianone G is indispensable to the activity Sophora flavescens diphosphate + sophoraflavanone G
-
?
dimethylallyl diphosphate + euchrenone a7 54% of the activity with leachianone G Sophora flavescens diphosphate + ?
-
?

Synonyms

Synonyms Comment Organism
leachianone G 2''-dimethylallyltransferase
-
Sophora flavescens
LG 2''-dimethylallyltransferase
-
Sophora flavescens
LGDT
-
Sophora flavescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Sophora flavescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
-
Sophora flavescens

pH Range

pH Minimum pH Maximum Comment Organism
7.9 10 50% of maximal activity within this range, almost inactive at pH 6.8, pH profile, overview Sophora flavescens