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Information on EC 2.4.1.183 - alpha-1,3-glucan synthase
for references in articles please use BRENDA:EC2.4.1.183
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EC Tree 2 Transferases
2.4 Glycosyltransferases
2.4.1 Hexosyltransferases
2.4.1.183 alpha-1,3-glucan synthase
IUBMB Comments
A glucan primer is needed to begin the reaction, which brings about elongation of the glucan chains.
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
- 2.4.1.183
- glucans
- beta-1,3-glucan
-
water-insoluble
- rho1
- brasiliensis
- sobrinus
-
dimorphic
- gtpases
- polysaccharide
- paracoccidioides
- niger
- mutans
- nidulans
-
1,6-alpha-d-glucan
- dextran
Reaction Schemes
showSynonyms
alpha-1,3-glucan synthase, 1,3-alpha-d-glucan synthase, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
1,3-alpha-D-glucan synthase
-
-
-
-
AbagsA
AbagsB
AbagsC
AbagsD
AbagsE
AGS
Ags1
AGS3
agsA
agsB
agsC
agsE
alpha-1,3-glucan synthase
glucosyltransferase, uridine diphosphoglucose-1,3-alpha-glucan
-
-
-
-
uridine diphosphoglucose-1,3-alpha-glucan glucosyltransferase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + [alpha-D-glucosyl-(1->3)]n = UDP + [alpha-D-glucosyl-(1->3)]n+1
a glucan primer is needed to start the reaction, which brings about elongation of the glucan chains
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hexosyl group transfer
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:alpha-D-(1->3)-glucan 3-alpha-D-glucosyltransferase
A glucan primer is needed to begin the reaction, which brings about elongation of the glucan chains.
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CAS REGISTRY NUMBER
COMMENTARY
113478-38-9
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-glucose + 2-(octadecylthio)-ethyl-beta-D-glucose
UDP + 2-(octadecylthio)-ethyl-beta-D-glucose-1,3-alpha-D-glucose
UDP-glucose + 2-(octadecylthio)-ethyl-beta-D-glucose
UDP + 2-(octadecylthio)-ethyl-beta-D-glucose-1,3-alpha-D-glucose
-
Substrates: synthetic acceptor composed of a monosaccharide linked to a long hydrophobic aliphatic chain, -CH2CH2SC18H37
Products: -
Products: -
?
UDP-glucose + 2-(octadecylthio)-ethyl-beta-D-glucose
UDP + 2-(octadecylthio)-ethyl-beta-D-glucose-1,3-alpha-D-glucose
-
Substrates: synthetic acceptor composed of a monosaccharide linked to a long hydrophobic aliphatic chain, -CH2CH2SC18H37
Products: -
Products: -
?
UDP-glucose + [alpha-D-glucosyl-(1-3)]n
UDP + [alpha-D-glucosyl-(1-3)]n+1
-
Substrates: substrate specificity
Products: product analysis
Products: product analysis
?
UDP-glucose + [alpha-D-glucosyl-(1-3)]n
UDP + [alpha-D-glucosyl-(1-3)]n+1
-
Substrates: enzyme forms alpha-1,3- and alpha-1,6-linkages onto the initial beta-D-glucose-phosphate
Products: product analysis
Products: product analysis
?
UDP-glucose + [alpha-D-glucosyl-(1-3)]n
UDP + [alpha-D-glucosyl-(1-3)]n+1
-
Substrates: enzyme EpsF: introduction of an alpha-1,3-linkage branch at the first beta-D-glucose-phosphate of the side-chain
Products: product analysis
Products: product analysis
?
UDP-glucose + [alpha-D-glucosyl-(1-3)]n
UDP + [alpha-D-glucosyl-(1-3)]n+1
-
Substrates: substrate specificity
Products: product analysis
Products: product analysis
?
UDP-glucose + [alpha-D-glucosyl-(1-3)]n
UDP + [alpha-D-glucosyl-(1-3)]n+1
-
Substrates: enzyme forms alpha-1,3- and alpha-1,6-linkages onto the initial beta-D-glucose-phosphate
Products: product analysis
Products: product analysis
?
UDP-glucose + [alpha-D-glucosyl-(1-3)]n
UDP + [alpha-D-glucosyl-(1-3)]n+1
-
Substrates: enzyme EpsF: introduction of an alpha-1,3-linkage branch at the first beta-D-glucose-phosphate of the side-chain
Products: product analysis
Products: product analysis
?
UDP-glucose + [alpha-D-glucosyl-(1-3)]n
UDP + [alpha-D-glucosyl-(1-3)]n+1
-
Substrates: enzyme catalyzes both the hydrolysis of sucrose to glucose and fructose and the glucosyl transfer to glucosyl polymers to yield water-insoluble glucan involving 2 different active sites, the enzyme catalyzes only sucrose hydrolysis however in the absence of 1,6-alpha-D-glucan as acceptor
Products: -
Products: -
?
UDP-glucose + [alpha-D-glucosyl-(1-3)]n
UDP + [alpha-D-glucosyl-(1-3)]n+1
-
Substrates: a glucan primer is needed to begin the reaction which brings about elongation of the glucan chains
Products: -
Products: -
?
UDP-glucose + [alpha-D-glucosyl-(1-3)]n
UDP + [alpha-D-glucosyl-(1-3)]n+1
-
Substrates: introduction of an alpha-1,3-linkage branch at the C3-position to the alpha-1,6-chain
Products: -
Products: -
?
UDP-glucose + [alpha-D-glucosyl-(1-3)]n
UDP + [alpha-D-glucosyl-(1-3)]n+1
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + [alpha-D-glucosyl-(1-3)]n
UDP + [alpha-D-glucosyl-(1-3)]n+1
-
Substrates: enzyme catalyzes both the hydrolysis of sucrose to glucose and fructose and the glucosyl transfer to glucosyl polymers to yield water-insoluble glucan involving 2 different active sites, the enzyme catalyzes only sucrose hydrolysis however in the absence of 1,6-alpha-D-glucan as acceptor
Products: -
Products: -
?
UDP-glucose + [alpha-D-glucosyl-(1-3)]n
UDP + [alpha-D-glucosyl-(1-3)]n+1
-
Substrates: a glucan primer is needed to begin the reaction which brings about elongation of the glucan chains
Products: -
Products: -
?
UDP-glucose + [alpha-D-glucosyl-(1-3)]n
UDP + [alpha-D-glucosyl-(1-3)]n+1
-
Substrates: introduction of an alpha-1,3-linkage branch at the C3-position to the alpha-1,6-chain
Products: -
Products: -
?
UDP-glucose + [alpha-D-glucosyl-(1-3)]n
UDP + [alpha-D-glucosyl-(1-3)]n+1
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + [alpha-D-glucosyl-(1->3)]n
UDP + [alpha-D-glucosyl-(1->3)]n+1
Substrates: -
Products: -
Products: -
?
UDP-glucose + [alpha-D-glucosyl-(1->3)]n
UDP + [alpha-D-glucosyl-(1->3)]n+1
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + [alpha-D-glucosyl-(1->3)]n
UDP + [alpha-D-glucosyl-(1->3)]n+1
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + [alpha-D-glucosyl-(1->3)]n
UDP + [alpha-D-glucosyl-(1->3)]n+1
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + [alpha-D-glucosyl-(1->3)]n
UDP + [alpha-D-glucosyl-(1->3)]n+1
Substrates: -
Products: -
Products: -
?
UDP-glucose + [alpha-D-glucosyl-(1->3)]n
UDP + [alpha-D-glucosyl-(1->3)]n+1
Substrates: -
Products: -
Products: -
?
UDP-glucose + [alpha-D-glucosyl-(1->3)]n
UDP + [alpha-D-glucosyl-(1->3)]n+1
Substrates: -
Products: -
Products: -
?
additional information
?
-
Substrates: expression of the agsA gene and to a lesser extent also of agsE is induced in the presence of the cell wall stress-inducing compounds such as Calco X uor White (CFW), SDS, and caspofungin. Induction of agsA in response to cell wall stress contributes to ensuring cell wall integrity
Products: -
Products: -
?
additional information
?
-
Substrates: expression of the agsA gene and to a lesser extent also of agsE is induced in the presence of the cell wall stress-inducing compounds such as Calco X uor White (CFW), SDS, and caspofungin. Induction of agsA in response to cell wall stress contributes to ensuring cell wall integrity
Products: -
Products: -
?
additional information
?
-
Substrates: expression of the agsA gene and to a lesser extent also of agsE is induced in the presence of the cell wall stress-inducing compounds such as Calco X uor White (CFW), SDS, and caspofungin. Induction of agsA in response to cell wall stress contributes to ensuring cell wall integrity
Products: -
Products: -
?
additional information
?
-
Substrates: expression of the agsA gene and to a lesser extent also of agsE is induced in the presence of the cell wall stress-inducing compounds such as Calco X uor White (CFW), SDS, and caspofungin. Induction of agsA in response to cell wall stress contributes to ensuring cell wall integrity
Products: -
Products: -
?
additional information
?
-
Substrates: expression of the agsA gene and to a lesser extent also of agsE are induced in the presence of the cell wall stress-inducing compounds such as Calco X uor White (CFW), SDS, and caspofungin. Induction of agsA in response to cell wall stress contributes to ensuring cell wall integrity
Products: -
Products: -
?
additional information
?
-
Substrates: expression of the agsA gene and to a lesser extent also of agsE are induced in the presence of the cell wall stress-inducing compounds such as Calco X uor White (CFW), SDS, and caspofungin. Induction of agsA in response to cell wall stress contributes to ensuring cell wall integrity
Products: -
Products: -
?
additional information
?
-
Substrates: expression of the agsA gene and to a lesser extent also of agsE are induced in the presence of the cell wall stress-inducing compounds such as Calco X uor White (CFW), SDS, and caspofungin. Induction of agsA in response to cell wall stress contributes to ensuring cell wall integrity
Products: -
Products: -
?
additional information
?
-
Substrates: expression of the agsA gene and to a lesser extent also of agsE are induced in the presence of the cell wall stress-inducing compounds such as Calco X uor White (CFW), SDS, and caspofungin. Induction of agsA in response to cell wall stress contributes to ensuring cell wall integrity
Products: -
Products: -
?
additional information
?
-
Substrates: expression of the agsA gene and to a lesser extent also of agsE are induced in the presence of the cell wall stress-inducing compounds such as Calco X uor White (CFW), SDS, and caspofungin. Induction of agsA in response to cell wall stress contributes to ensuring cell wall integrity
Products: -
Products: -
?
additional information
?
-
Substrates: expression of the agsA gene and to a lesser extent also of agsE are induced in the presence of the cell wall stress-inducing compounds such as Calco X uor White (CFW), SDS, and caspofungin. Induction of agsA in response to cell wall stress contributes to ensuring cell wall integrity
Products: -
Products: -
?
additional information
?
-
Substrates: expression of the agsA gene and to a lesser extent also of agsE are induced in the presence of the cell wall stress-inducing compounds such as Calco X uor White (CFW), SDS, and caspofungin. Induction of agsA in response to cell wall stress contributes to ensuring cell wall integrity
Products: -
Products: -
?
additional information
?
-
Substrates: expression of the agsA gene and to a lesser extent also of agsE are induced in the presence of the cell wall stress-inducing compounds such as Calco X uor White (CFW), SDS, and caspofungin. Induction of agsA in response to cell wall stress contributes to ensuring cell wall integrity
Products: -
Products: -
?
additional information
?
-
Substrates: expression of the agsA gene and to a lesser extent also of agsE is induced in the presence of the cell wall stress-inducing compounds such as Calco X uor White (CFW), SDS, and caspofungin. Induction of agsA in response to cell wall stress contributes to ensuring cell wall integrity
Products: -
Products: -
?
additional information
?
-
Substrates: expression of the agsA gene and to a lesser extent also of agsE is induced in the presence of the cell wall stress-inducing compounds such as Calco X uor White (CFW), SDS, and caspofungin. Induction of agsA in response to cell wall stress contributes to ensuring cell wall integrity
Products: -
Products: -
?
additional information
?
-
Substrates: expression of the agsA gene and to a lesser extent also of agsE is induced in the presence of the cell wall stress-inducing compounds such as Calco X uor White (CFW), SDS, and caspofungin. Induction of agsA in response to cell wall stress contributes to ensuring cell wall integrity
Products: -
Products: -
?
additional information
?
-
Substrates: expression of the agsA gene and to a lesser extent also of agsE is induced in the presence of the cell wall stress-inducing compounds such as Calco X uor White (CFW), SDS, and caspofungin. Induction of agsA in response to cell wall stress contributes to ensuring cell wall integrity
Products: -
Products: -
?
additional information
?
-
-
Substrates: no activity with p-aminophenyl, p-nitrophenyl, and 6-(fluorescein-5-carboxamido)-hexanoic acid succimidyl ester
Products: -
Products: -
?
additional information
?
-
-
Substrates: no activity with p-aminophenyl, p-nitrophenyl, and 6-(fluorescein-5-carboxamido)-hexanoic acid succimidyl ester
Products: -
Products: -
?
additional information
?
-
Substrates: induction of 1,3-alpha D-glucan synthase-encoding genes in response to cell wall stress
Products: -
Products: -
?
additional information
?
-
Substrates: induction of 1,3-alpha D-glucan synthase-encoding genes in response to cell wall stress
Products: -
Products: -
?
additional information
?
-
Substrates: induction of 1,3-alpha D-glucan synthase-encoding genes in response to cell wall stress
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-glucose + [alpha-D-glucosyl-(1-3)]n
UDP + [alpha-D-glucosyl-(1-3)]n+1
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + [alpha-D-glucosyl-(1-3)]n
UDP + [alpha-D-glucosyl-(1-3)]n+1
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + [alpha-D-glucosyl-(1->3)]n
UDP + [alpha-D-glucosyl-(1->3)]n+1
Substrates: -
Products: -
Products: -
?
UDP-glucose + [alpha-D-glucosyl-(1->3)]n
UDP + [alpha-D-glucosyl-(1->3)]n+1
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + [alpha-D-glucosyl-(1->3)]n
UDP + [alpha-D-glucosyl-(1->3)]n+1
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + [alpha-D-glucosyl-(1->3)]n
UDP + [alpha-D-glucosyl-(1->3)]n+1
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + [alpha-D-glucosyl-(1->3)]n
UDP + [alpha-D-glucosyl-(1->3)]n+1
Substrates: -
Products: -
Products: -
?
UDP-glucose + [alpha-D-glucosyl-(1->3)]n
UDP + [alpha-D-glucosyl-(1->3)]n+1
Substrates: -
Products: -
Products: -
?
UDP-glucose + [alpha-D-glucosyl-(1->3)]n
UDP + [alpha-D-glucosyl-(1->3)]n+1
Substrates: -
Products: -
Products: -
?
additional information
?
-
Substrates: expression of the agsA gene and to a lesser extent also of agsE is induced in the presence of the cell wall stress-inducing compounds such as Calco X uor White (CFW), SDS, and caspofungin. Induction of agsA in response to cell wall stress contributes to ensuring cell wall integrity
Products: -
Products: -
?
additional information
?
-
Substrates: expression of the agsA gene and to a lesser extent also of agsE is induced in the presence of the cell wall stress-inducing compounds such as Calco X uor White (CFW), SDS, and caspofungin. Induction of agsA in response to cell wall stress contributes to ensuring cell wall integrity
Products: -
Products: -
?
additional information
?
-
Substrates: expression of the agsA gene and to a lesser extent also of agsE is induced in the presence of the cell wall stress-inducing compounds such as Calco X uor White (CFW), SDS, and caspofungin. Induction of agsA in response to cell wall stress contributes to ensuring cell wall integrity
Products: -
Products: -
?
additional information
?
-
Substrates: expression of the agsA gene and to a lesser extent also of agsE is induced in the presence of the cell wall stress-inducing compounds such as Calco X uor White (CFW), SDS, and caspofungin. Induction of agsA in response to cell wall stress contributes to ensuring cell wall integrity
Products: -
Products: -
?
additional information
?
-
Substrates: expression of the agsA gene and to a lesser extent also of agsE are induced in the presence of the cell wall stress-inducing compounds such as Calco X uor White (CFW), SDS, and caspofungin. Induction of agsA in response to cell wall stress contributes to ensuring cell wall integrity
Products: -
Products: -
?
additional information
?
-
Substrates: expression of the agsA gene and to a lesser extent also of agsE are induced in the presence of the cell wall stress-inducing compounds such as Calco X uor White (CFW), SDS, and caspofungin. Induction of agsA in response to cell wall stress contributes to ensuring cell wall integrity
Products: -
Products: -
?
additional information
?
-
Substrates: expression of the agsA gene and to a lesser extent also of agsE are induced in the presence of the cell wall stress-inducing compounds such as Calco X uor White (CFW), SDS, and caspofungin. Induction of agsA in response to cell wall stress contributes to ensuring cell wall integrity
Products: -
Products: -
?
additional information
?
-
Substrates: expression of the agsA gene and to a lesser extent also of agsE are induced in the presence of the cell wall stress-inducing compounds such as Calco X uor White (CFW), SDS, and caspofungin. Induction of agsA in response to cell wall stress contributes to ensuring cell wall integrity
Products: -
Products: -
?
additional information
?
-
Substrates: expression of the agsA gene and to a lesser extent also of agsE are induced in the presence of the cell wall stress-inducing compounds such as Calco X uor White (CFW), SDS, and caspofungin. Induction of agsA in response to cell wall stress contributes to ensuring cell wall integrity
Products: -
Products: -
?
additional information
?
-
Substrates: expression of the agsA gene and to a lesser extent also of agsE are induced in the presence of the cell wall stress-inducing compounds such as Calco X uor White (CFW), SDS, and caspofungin. Induction of agsA in response to cell wall stress contributes to ensuring cell wall integrity
Products: -
Products: -
?
additional information
?
-
Substrates: expression of the agsA gene and to a lesser extent also of agsE are induced in the presence of the cell wall stress-inducing compounds such as Calco X uor White (CFW), SDS, and caspofungin. Induction of agsA in response to cell wall stress contributes to ensuring cell wall integrity
Products: -
Products: -
?
additional information
?
-
Substrates: expression of the agsA gene and to a lesser extent also of agsE are induced in the presence of the cell wall stress-inducing compounds such as Calco X uor White (CFW), SDS, and caspofungin. Induction of agsA in response to cell wall stress contributes to ensuring cell wall integrity
Products: -
Products: -
?
additional information
?
-
Substrates: expression of the agsA gene and to a lesser extent also of agsE is induced in the presence of the cell wall stress-inducing compounds such as Calco X uor White (CFW), SDS, and caspofungin. Induction of agsA in response to cell wall stress contributes to ensuring cell wall integrity
Products: -
Products: -
?
additional information
?
-
Substrates: expression of the agsA gene and to a lesser extent also of agsE is induced in the presence of the cell wall stress-inducing compounds such as Calco X uor White (CFW), SDS, and caspofungin. Induction of agsA in response to cell wall stress contributes to ensuring cell wall integrity
Products: -
Products: -
?
additional information
?
-
Substrates: expression of the agsA gene and to a lesser extent also of agsE is induced in the presence of the cell wall stress-inducing compounds such as Calco X uor White (CFW), SDS, and caspofungin. Induction of agsA in response to cell wall stress contributes to ensuring cell wall integrity
Products: -
Products: -
?
additional information
?
-
Substrates: expression of the agsA gene and to a lesser extent also of agsE is induced in the presence of the cell wall stress-inducing compounds such as Calco X uor White (CFW), SDS, and caspofungin. Induction of agsA in response to cell wall stress contributes to ensuring cell wall integrity
Products: -
Products: -
?
additional information
?
-
Substrates: induction of 1,3-alpha D-glucan synthase-encoding genes in response to cell wall stress
Products: -
Products: -
?
additional information
?
-
Substrates: induction of 1,3-alpha D-glucan synthase-encoding genes in response to cell wall stress
Products: -
Products: -
?
additional information
?
-
Substrates: induction of 1,3-alpha D-glucan synthase-encoding genes in response to cell wall stress
Products: -
Products: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Antiserum against the isolated 1,3-alpha-D-glucan synthase
-
glucosyl transfer activity is completely inhibited, not sucrose hydrolysis activity
-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
i.e. Emericella nidulans, genes agsA and agsB
UniProt
brenda
G10 a spontaneous nitrate reductase mutant of strain CBS 144-89
-
-
brenda
Aspergillus niger responds to cell wall stress by transcriptional activation of cell wall reinforcing genes including agsA (encoding a alpha-1,3-glucan synthase) through an RIm1p-like transcription factor.
-
-
brenda
Aspergillus niger responds to cell wall stress by transcriptional activation of cell wall reinforcing genes including agsA (encoding a alpha-1,3-glucan synthase) through an RIm1p-like transcription factor.
-
-
brenda
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
additional information
additional information
-
alpha-1,3-glucan is located in the inner layer in the agsA overexpressing strain
-
brenda
additional information
-
alpha-1,3-glucan is located in the outer layer of the cell wall in the agsB overexpressing strain
-
brenda
additional information
-
alpha-1,3-glucan is located in the inner layer in the agsA overexpressing strain
-
-
brenda
additional information
-
alpha-1,3-glucan is located in the outer layer of the cell wall in the agsB overexpressing strain
-
-
brenda
additional information
-
alpha-1,3-glucan is located in the outer layer of the cell wall in the agsB overexpressing strain
-
brenda
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
evolution
AgsE is one of five alpha-1,3-glucan synthase genes in Aspergillus luchuensis and a homologue of the major alpha-1,3-glucan synthase agsB in Aspergillus nidulans
malfunction
physiological function
additional information
malfunction
alpha-1,3-glucan is the main component of the alkali-soluble cell wall fraction in the wild-type and agsA disruption strains, but almost no alpha-1,3-glucan is found in the alkali-soluble fraction derived from either the agsB disruption strain or the CagsB strain under the agsB-repressed conditions, regardless of the agsA genetic background. Hyphal morphology of the control and agsB disruption strains, overview
malfunction
deletion of all the three AGS genes results in a triple mutant that is devoid of alpha-(1,3)-glucan in its cell wall, buts growth and germination is identical to that of the parental strain in vitro. In the experimental murine aspergillosis model, this mutant is less pathogenic than the parental strain. The AGS deletion results in an extensive structural modification of the conidial cell wall, especially conidial surface where the rodlet layer is covered by an amorphous glycoprotein matrix. Thie surface modification is responsible for viability reduction of conidia in vivo, which explains decrease in the virulence of triple agsD mutant
malfunction
deletion of all the three AGS genes results in a triple mutant that is devoid of alpha-(1,3)-glucan in its cell wall, buts growth and germination is identical to that of the parental strain in vitro. In the experimental murine aspergillosis model, this mutant is less pathogenic than the parental strain. The AGS deletion results in an extensive structural modification of the conidial cell wall, especially conidial surface where the rodlet layer is covered by an amorphous glycoprotein matrix. The surface modification is responsible for viability reduction of conidia in vivo, which explains decrease in the virulence of triple agsD mutant
malfunction
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the disruption of the agsA gene does not alter or slightly increase the alpha-1,3-glucan content compared to wild-type. Overexpression of agsA increases the amount of alkali-soluble glucan compared to wild-type. Alkali-soluble glucan from the agsA overexpressing strain is composed mainly of alpha-1,3-glucan (1,3,5-triacetyl-2,4,6-tri-O-methyl-D-glucitol). The average molecular mass of alkali-soluble glucan is larger in agsA overexpressing than in agsB overespressing strains. The double mutant DELTAagsA-DELTAagsB strain lacks cell wall alpha-1,3-glucan
malfunction
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the disruption of the agsB gene leads to loss of alpha-1,3-glucan. Overexpression of agsA increases the amount of alkali-soluble glucan compared to wild-type. Alkali-soluble glucan from the agsB overexpressing strain is composed mainly of alpha-1,3-glucan (1,3,5-triacetyl-2,4,6-tri-O-methyl-D-glucitol). The double mutant DELTAagsA-DELTAagsB strain lacks cell wall alpha-1,3-glucan
malfunction
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the disruption of the agsB gene leads to the loss of alpha-1,3-glucan. Overexpression of agsB increases the amount of alkali-soluble glucan compared to wild-type. Alkali-soluble glucan from the agsB overexpressing strain is composed mainly of alpha-1,3-glucan. The average molecular mass of alkali-soluble glucan is larger in agsA overexpressing than in agsB overespressing strains
malfunction
disruption of the genes encoding cell wall alpha-1,3-glucan synthase leads to increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae. The amount of CutL1 secreted by the tripleDELTA-cutL1 and quintupleDELTA- cutL1 strains iapproximately twice that produced by the wild-type-cutL1 strain
malfunction
disruption of the genes encoding cell wall alpha-1,3-glucan synthase leads to increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae. The amount of CutL1 secreted by the tripleDELTA-cutL1 and quintupleDELTA- cutL1 strains is approximately twice that produced by the wild-type-cutL1 strain
malfunction
disruption of agsE in Aspergillus luchuensis strain NBRC 4314 (DELTAagsE) shows that protoplast formation in DELTAagsE is comparable with protoplast formation in Aspergillus oryzae with commercial cell wall-digesting enzyme Yatalase. The DELTAagsE protoplasts are also competent for transformation with the protoplast-PEG method
malfunction
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overexpression of Aspergillus nidulans alpha-1,3-glucan synthase increases cellular adhesion and causes cell wall defects
malfunction
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ags1 gene deletion reduces mycelial aggregation, ags1 gene overexpression increases mycelial aggregation. The exopolysaccharide content is significantly higher in ags1 deletion mutants and lower in ags1 overexpreessing strains, whereas the mycelial polysaccharide contents show the opposite trend
malfunction
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disruption of the genes encoding cell wall alpha-1,3-glucan synthase leads to increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae. The amount of CutL1 secreted by the tripleDELTA-cutL1 and quintupleDELTA- cutL1 strains iapproximately twice that produced by the wild-type-cutL1 strain
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malfunction
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disruption of the genes encoding cell wall alpha-1,3-glucan synthase leads to increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae. The amount of CutL1 secreted by the tripleDELTA-cutL1 and quintupleDELTA- cutL1 strains is approximately twice that produced by the wild-type-cutL1 strain
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malfunction
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the disruption of the agsA gene does not alter or slightly increase the alpha-1,3-glucan content compared to wild-type. Overexpression of agsA increases the amount of alkali-soluble glucan compared to wild-type. Alkali-soluble glucan from the agsA overexpressing strain is composed mainly of alpha-1,3-glucan (1,3,5-triacetyl-2,4,6-tri-O-methyl-D-glucitol). The average molecular mass of alkali-soluble glucan is larger in agsA overexpressing than in agsB overespressing strains. The double mutant DELTAagsA-DELTAagsB strain lacks cell wall alpha-1,3-glucan
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malfunction
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the disruption of the agsB gene leads to loss of alpha-1,3-glucan. Overexpression of agsA increases the amount of alkali-soluble glucan compared to wild-type. Alkali-soluble glucan from the agsB overexpressing strain is composed mainly of alpha-1,3-glucan (1,3,5-triacetyl-2,4,6-tri-O-methyl-D-glucitol). The double mutant DELTAagsA-DELTAagsB strain lacks cell wall alpha-1,3-glucan
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malfunction
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disruption of the genes encoding cell wall alpha-1,3-glucan synthase leads to increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae. The amount of CutL1 secreted by the tripleDELTA-cutL1 and quintupleDELTA- cutL1 strains iapproximately twice that produced by the wild-type-cutL1 strain
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malfunction
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disruption of the genes encoding cell wall alpha-1,3-glucan synthase leads to increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae. The amount of CutL1 secreted by the tripleDELTA-cutL1 and quintupleDELTA- cutL1 strains is approximately twice that produced by the wild-type-cutL1 strain
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malfunction
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alpha-1,3-glucan is the main component of the alkali-soluble cell wall fraction in the wild-type and agsA disruption strains, but almost no alpha-1,3-glucan is found in the alkali-soluble fraction derived from either the agsB disruption strain or the CagsB strain under the agsB-repressed conditions, regardless of the agsA genetic background. Hyphal morphology of the control and agsB disruption strains, overview
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malfunction
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ags1 gene deletion reduces mycelial aggregation, ags1 gene overexpression increases mycelial aggregation. The exopolysaccharide content is significantly higher in ags1 deletion mutants and lower in ags1 overexpreessing strains, whereas the mycelial polysaccharide contents show the opposite trend
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physiological function
the enzyme is involved in the cell wall biogenesis. Alteration of the alpha-1,3-glucan amount is counterbalanced by alterations in the amount of another cell wall component such as beta-1,3-glucan or chitin
physiological function
the enzyme is involved in biosynthesis of alpha-1,3-glucan and the cell wall biogenesis. alpha-(1,3)-Glucan is a major cell wall component of most ascomycetous and basidiomycetous fungi, including the human pathogens. In Aspergillus fumigatus, alpha-(1,3)-glucan is a key component of the extracellular matrix, which encloses the cell wall beta-(1,3)-glucan-chitin fibrillar core
physiological function
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alpha-1,3-glucan is one of the main polysaccharides in the cell wall of filamentous fungi. Aspergillus nidulans has two alpha-1,3-glucan synthase genes, agsA and agsB. AgsB is a major alpha-1,3-glucan synthase in vegetative hyphae, but the function of AgsA is intracellular
physiological function
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alpha-1,3-glucan is one of the main polysaccharides in the cell wall of filamentous fungi. Aspergillus nidulans has two alpha-1,3-glucan synthase genes, agsA and agsB. AgsB is a major alpha-1,3-glucan synthase in vegetative hyphae, but the function of AgsA is intracellular. alpha-1,3-Glucan in vegetative hyphae is synthesized mainly by AgsB. AgsB is required for alpha-1,3 glucan biosynthesis under normal growth conditions
physiological function
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alpha-1,3-glucan is one of the main polysaccharides in the cell wall of filamentous fungi. Aspergillus nidulans has two alpha-1,3-glucan synthase genes, agsA and agsB. AgsB is a major alpha-1,3-glucan synthase in vegetative hyphae, but the function of AgsA is intracellular. alpha-1,3-Glucan in vegetative hyphae is synthesized mainly by AgsB. AgsB is required for alpha-1,3 glucan biosynthesis under normal growth conditions
physiological function
the ags genes play a major role in alpha-1,3-glucan biosynthesis in Aspergillus oryzae, while the amyD and amyG genes do not
physiological function
role of the alpha-1,3-glucan synthase gene agsE in protoplast formation: agsE appears to inhibit protoplast formation in Aspergillus luchuensis, overview
physiological function
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alpha-1,3-glucan has important roles in fungal cellular adhesion and may contribute to fungal pathogenesis
physiological function
in a strain producing free dihomo-gamma-linolenic acid at an increased yield by faaA gene disruption and cooverexpression of one elongase and two desaturase genes, disruption of gene agsB further increases yield by 1.3-fold to 533 mg/l
physiological function
deletion of AgsE decreases the pH of culture medium from 5.2 in the case of the wild-type to 6.4 for the deletion strain. The diameter of microcolonies is reduced from 3304 microm for wild-type to 1229 microm for the deletion strain. The secretome shows 54 unique proteins with a predicted signal peptide in wild-type, 36 in the mutant. Biomass is similar in both strains
physiological function
removing AgsE increases sensitivity to CR and CFW. The deletion results in dispersed homogenous growth characteristics with a visible decrease in viscosity over time. Deleting the entire Ags gene family does not further affect cell wall integrity compared to the AgsE single mutant
physiological function
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the ags genes play a major role in alpha-1,3-glucan biosynthesis in Aspergillus oryzae, while the amyD and amyG genes do not
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physiological function
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alpha-1,3-glucan is one of the main polysaccharides in the cell wall of filamentous fungi. Aspergillus nidulans has two alpha-1,3-glucan synthase genes, agsA and agsB. AgsB is a major alpha-1,3-glucan synthase in vegetative hyphae, but the function of AgsA is intracellular
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physiological function
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alpha-1,3-glucan is one of the main polysaccharides in the cell wall of filamentous fungi. Aspergillus nidulans has two alpha-1,3-glucan synthase genes, agsA and agsB. AgsB is a major alpha-1,3-glucan synthase in vegetative hyphae, but the function of AgsA is intracellular. alpha-1,3-Glucan in vegetative hyphae is synthesized mainly by AgsB. AgsB is required for alpha-1,3 glucan biosynthesis under normal growth conditions
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physiological function
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the ags genes play a major role in alpha-1,3-glucan biosynthesis in Aspergillus oryzae, while the amyD and amyG genes do not
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physiological function
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in a strain producing free dihomo-gamma-linolenic acid at an increased yield by faaA gene disruption and cooverexpression of one elongase and two desaturase genes, disruption of gene agsB further increases yield by 1.3-fold to 533 mg/l
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physiological function
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the enzyme is involved in the cell wall biogenesis. Alteration of the alpha-1,3-glucan amount is counterbalanced by alterations in the amount of another cell wall component such as beta-1,3-glucan or chitin
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additional information
expression profiles of genes involved in cell wall biosynthesis. Levels of transcription of several cell wall-related genes (gelA, chsA, and gfaA) are significantly upregulated in the agsB disruption strain, transcription of gelB and csmB is significantly downregulated in the disruptant. In the CagsB strain, the expression of genes agsA, fksA, gelA, chsA, and gfaA is significantly upregulated under the agsB-repressing conditions, as is the case in the agsB disruption strain for all except fksA. In contrast, under the agsB-inducing conditions, the expression levels of fksA, gelB, chsD, and gfaA are significantly reduced in the CagsB strain, gene csmA is significantly upregulated under these conditions
additional information
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determination of the average molecular mass of polysaccharides in cell extracts, methylation analysis of alkali-soluble glucan, overview. alpha-1,3-Glucan is located in the inner layer in the agsA overexpressing strain
additional information
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determination of the average molecular mass of polysaccharides in cell extracts, methylation analysis of alkali-soluble glucan, overview. alpha-1,3-Glucan is located in the outer layer of the cell wall in the agsB overexpressing strain
additional information
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determination of the average molecular mass of polysaccharides in cell extracts, methylation analysis of alkali-soluble glucan, overview. alpha-1,3-Glucan is located in the outer layer of the cell wall in the agsB overexpressing strain
additional information
analysis of the monosaccharide composition of the cell walls from wild-type, tripleDELTA (agsADELTAagsBDELTAagsCDELTA), and quintupleDELTA (agsADELTAagsBDELTAagsCDELTAamyGDELTA), lyophilized hyphal cells of each strain of Aspergillus oryzae, overview. The HW fraction mainly contains galactose and mannose and the AS1 fraction mainly contains glucose and mannose. The alkali-soluble 2 (AS2) fraction derived from Aspergillus oryzae wild-type mainly contains glucose (approx. 20% of the AS2 fraction) with a small amount of mannose. But those derived from Aspergillus oryzae tripleDELTA and quintupleDELTA contain significantly less glucose. The alkali-soluble 1 (A1) fraction derived from each strain contains glucose, glucosamine, and mannose. The glucose and glucosamine contents of the A1 fraction derived from each strain are around 28% and 20% of the total dry weight of the A1 fraction, respectively
additional information
analysis of the monosaccharide composition of the cell walls from wild-type, tripleDELTA (agsADELTAagsBDELTAagsCDELTA), and quintupleDELTA (agsADELTAagsBDELTAagsCDELTAamyGDELTA), lyophilized hyphal cells of each strain of Aspergillus oryzae, overview. The HW fraction mainly contains galactose and mannose and the AS1 fraction mainly contains glucose and mannose. The alkali-soluble 2 (AS2) fraction derived from Aspergillus oryzae wild-type mainly contains glucose (approx. 20% of the AS2 fraction) with a small amount of mannose. But those derived from Aspergillus oryzae tripleDELTA and quintupleDELTA contain significantly less glucose. The alkali-soluble 1 (A1) fraction derived from each strain contains glucose, glucosamine, and mannose. The glucose and glucosamine contents of the A1 fraction derived from each strain are around 28% and 20% of the total dry weight of the A1 fraction, respectively
additional information
analysis of the monosaccharide composition of the cell walls from wild-type, tripleDELTA (agsADELTAagsBDELTAagsCDELTA), and quintupleDELTA (agsADELTAagsBDELTAagsCDELTAamyGDELTA), lyophilized hyphal cells of each strain of Aspergillus oryzae, overview. The HW fraction mainly contains galactose and mannose and the AS1 fraction mainly contains glucose and mannose. The alkali-soluble 2 (AS2) fraction derived from Aspergillus oryzae wild-type mainly contains glucose (approx. 20% of the AS2 fraction) with a small amount of mannose. But those derived from Aspergillus oryzae tripleDELTA and quintupleDELTA contain significantly less glucose. The alkali-soluble 1 (A1) fraction derived from each strain contains glucose, glucosamine, and mannose. The glucose and glucosamine contents of the A1 fraction derived from each strain are around 28% and 20% of the total dry weight of the A1 fraction, respectively
additional information
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analysis of the monosaccharide composition of the cell walls from wild-type, tripleDELTA (agsADELTAagsBDELTAagsCDELTA), and quintupleDELTA (agsADELTAagsBDELTAagsCDELTAamyGDELTA), lyophilized hyphal cells of each strain of Aspergillus oryzae, overview. The HW fraction mainly contains galactose and mannose and the AS1 fraction mainly contains glucose and mannose. The alkali-soluble 2 (AS2) fraction derived from Aspergillus oryzae wild-type mainly contains glucose (approx. 20% of the AS2 fraction) with a small amount of mannose. But those derived from Aspergillus oryzae tripleDELTA and quintupleDELTA contain significantly less glucose. The alkali-soluble 1 (A1) fraction derived from each strain contains glucose, glucosamine, and mannose. The glucose and glucosamine contents of the A1 fraction derived from each strain are around 28% and 20% of the total dry weight of the A1 fraction, respectively
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additional information
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determination of the average molecular mass of polysaccharides in cell extracts, methylation analysis of alkali-soluble glucan, overview. alpha-1,3-Glucan is located in the inner layer in the agsA overexpressing strain
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additional information
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analysis of the monosaccharide composition of the cell walls from wild-type, tripleDELTA (agsADELTAagsBDELTAagsCDELTA), and quintupleDELTA (agsADELTAagsBDELTAagsCDELTAamyGDELTA), lyophilized hyphal cells of each strain of Aspergillus oryzae, overview. The HW fraction mainly contains galactose and mannose and the AS1 fraction mainly contains glucose and mannose. The alkali-soluble 2 (AS2) fraction derived from Aspergillus oryzae wild-type mainly contains glucose (approx. 20% of the AS2 fraction) with a small amount of mannose. But those derived from Aspergillus oryzae tripleDELTA and quintupleDELTA contain significantly less glucose. The alkali-soluble 1 (A1) fraction derived from each strain contains glucose, glucosamine, and mannose. The glucose and glucosamine contents of the A1 fraction derived from each strain are around 28% and 20% of the total dry weight of the A1 fraction, respectively
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additional information
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expression profiles of genes involved in cell wall biosynthesis. Levels of transcription of several cell wall-related genes (gelA, chsA, and gfaA) are significantly upregulated in the agsB disruption strain, transcription of gelB and csmB is significantly downregulated in the disruptant. In the CagsB strain, the expression of genes agsA, fksA, gelA, chsA, and gfaA is significantly upregulated under the agsB-repressing conditions, as is the case in the agsB disruption strain for all except fksA. In contrast, under the agsB-inducing conditions, the expression levels of fksA, gelB, chsD, and gfaA are significantly reduced in the CagsB strain, gene csmA is significantly upregulated under these conditions
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
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deltaags3, with a non-functional, disrupted AGS3 gene. The cell wall composition of the mycelium or the conidia of the deltaags3 mutant and the wild type strain does not present any qualitive or quantitive differences. The dense outer layer surrounding the conidial cell wall that contains melanin is 2times thicker in the deltaags3 mutants than in the wild type. Deletion of AGS1 results in a 2.8 and 1.9fold increase in the expression of AGS1 and AGS2, respectively. Lung invasion in infected mice is significantly higher in the mutant than in the wild type. The mutant has a better resistance to reactive oxygen species and a quicker germination rate
additional information
deletion of all the three AGS genes, the ags1DELTAags2DELTAags3DELTA (agsDELTA) mutants are less virulent than the parental strain in murine model of aspergillosis, mutant phenotypes, overview. Susceptibility of the agsDELTA and parental strain conidia to antifungal molecules is similar. The resting conidia of the agsDELTA mutants are immediately recognized by the innate immune system of mice because the surface rodlet layer is masked by a layer of glycoproteins, overview
additional information
deletion of all the three AGS genes, the ags1DELTAags2DELTAags3DELTA (agsDELTA) mutants are less virulent than the parental strain in murine model of aspergillosis, mutant phenotypes, overview. Susceptibility of the agsDELTA and parental strain conidia to antifungal molecules is similar. The resting conidia of the agsDELTA mutants are immediately recognized by the innate immune system of mice because the surface rodlet layer is masked by a layer of glycoproteins, overview
additional information
deletion of all the three AGS genes, the ags1DELTAags2DELTAags3DELTA (agsDELTA) mutants are less virulent than the parental strain in murine model of aspergillosis, mutant phenotypes, overview. Susceptibility of the agsDELTA and parental strain conidia to antifungal molecules is similar. The resting conidia of the agsDELTA mutants are immediately recognized by the innate immune system of mice because the surface rodlet layer is masked by a layer of glycoproteins, overview
additional information
disruption of gene agsE in Aspergillus luchuensis (DELTAagsE) with Agrobacterium-mediated transformation (AMT) . The DELTAagsE protoplasts are also competent for transformation with the protoplast-PEG method. Phenotypes of the agsE disruptant, overview
additional information
construction of several mutant strains in Aspergillus nidulans by gene replacement: agsA disruption, agsB disruption, and double-disruption strains, as well asl CagsB strains in which agsB expression is controlled by the inducible alcA promoter, with or without the agsA-disrupting mutation. The agsA disruption strains do not show markedly different phenotypes from the wild-type strain. The agsB disruption strains forms dispersed hyphal cells under liquid culture conditions, regardless of the agsA genetic background. Dispersed hyphal cells are also observed in liquid culture of the CagsB strains when agsB expression is repressed, whereas these strains grow normally in plate culture even under the agsB-repressed conditions. Phenotypes, overview
additional information
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overexpression of agsA in Aspergillus nidulans under the control of a constitutive promoter in the genetic background of agsB disruptants, the native promoter is replaced by the constitutive tef1 promoter. Alkali-soluble glucan from the agsA overexpressing strain is composed mainly of alpha-1,3-glucan. The agsA gene is disrupted by using the Cre/loxP marker recycling system. Generation of an agsA deletion mutant strain agsADELTA. Genetic structure of mutant strains, overview. The double mutant DELTAagsA-DELTAagsB strain lacks cell wall alpha-1,3-glucan
additional information
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overexpression of agsB in Aspergillus nidulans under control of a constitutive promoter in the genetic background of agsA disruptants, the native promoter is replaced by the constitutive tef1 promoter. Generation of an agsB deletion mutant strain agsBDELTA. Genetic structure of mutant strains, overview. The double mutant DELTAagsA-DELTAagsB strain lacks cell wall alpha-1,3-glucan
additional information
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generation of an Aspergillus nidulans alpha-1,3-glucan in constitutive overexpression strain. Constitutive high-level alpha-1,3-glucan synthase activity increases hyphal wall thickness, but colonies grow slowly and sporulate poorly and have much higher adhesion to hydrophobic materials. In contrast to the wild-type strain, the overexpression strain forms a biofilm-like structure in plastic culture wells that is as adhesive as wild-type Aspergillus fumigatus. The high level of agsB expression is deleterious. Phenotype, overview
additional information
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overexpression of agsA in Aspergillus nidulans under the control of a constitutive promoter in the genetic background of agsB disruptants, the native promoter is replaced by the constitutive tef1 promoter. Alkali-soluble glucan from the agsA overexpressing strain is composed mainly of alpha-1,3-glucan. The agsA gene is disrupted by using the Cre/loxP marker recycling system. Generation of an agsA deletion mutant strain agsADELTA. Genetic structure of mutant strains, overview. The double mutant DELTAagsA-DELTAagsB strain lacks cell wall alpha-1,3-glucan
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additional information
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overexpression of agsB in Aspergillus nidulans under control of a constitutive promoter in the genetic background of agsA disruptants, the native promoter is replaced by the constitutive tef1 promoter. Generation of an agsB deletion mutant strain agsBDELTA. Genetic structure of mutant strains, overview. The double mutant DELTAagsA-DELTAagsB strain lacks cell wall alpha-1,3-glucan
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additional information
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construction of several mutant strains in Aspergillus nidulans by gene replacement: agsA disruption, agsB disruption, and double-disruption strains, as well asl CagsB strains in which agsB expression is controlled by the inducible alcA promoter, with or without the agsA-disrupting mutation. The agsA disruption strains do not show markedly different phenotypes from the wild-type strain. The agsB disruption strains forms dispersed hyphal cells under liquid culture conditions, regardless of the agsA genetic background. Dispersed hyphal cells are also observed in liquid culture of the CagsB strains when agsB expression is repressed, whereas these strains grow normally in plate culture even under the agsB-repressed conditions. Phenotypes, overview
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additional information
disruption of the genes encoding cell wall alpha-1,3-glucan synthases, i.e. agsA, agsB, agsC, in Aspergillus oryzae strain NS4 (sC-, niaD-) leads to increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae. Transformation of the cutinase-encoding gene cutL1 into Aspergillus oryzae wild-type and the tripleDELTA mutant showing that tripleDELTA-cutL1 forms smaller hyphal pellets and shows both greater biomass and increased CutL1 productivity compared to wild-type-cutL1, which might be attributable to a decrease in the number of tripleDELTA-cutL1 cells under anaerobic conditions. Mutant phenotypes, overview. Analysis of the monosaccharide composition of the cell walls from wild-type, tripleDELTA (agsADELTAagsBDELTAagsCDELTA), and quintupleDELTA (agsADELTAagsBDELTAagsCDELTAamyGDELTA), lyophilized hyphal cells of each strain of Aspergillus oryzae, overview. The HW fraction mainly contains galactose and mannose and the AS1 fraction mainly contains glucose and mannose. The alkali-soluble 2 (AS2) fraction derived from Aspergillus oryzae wild-type mainly contains glucose (approx. 20% of the AS2 fraction) with a small amount of mannose. But those derived from Aspergillus oryzae tripleDELTA and quintupleDELTA contain significantly less glucose. The alkali-soluble 1 (A1) fraction derived from each strain contains glucose, glucosamine, and mannose. The glucose and glucosamine contents of the A1 fraction derived from each strain are around 28% and 20% of the total dry weight of the A1 fraction, respectively
additional information
disruption of the genes encoding cell wall alpha-1,3-glucan synthases, i.e. agsA, agsB, agsC, in Aspergillus oryzae strain NS4 (sC-, niaD-) leads to increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae. Transformation of the cutinase-encoding gene cutL1 into Aspergillus oryzae wild-type and the tripleDELTA mutant showing that tripleDELTA-cutL1 forms smaller hyphal pellets and shows both greater biomass and increased CutL1 productivity compared to wild-type-cutL1, which might be attributable to a decrease in the number of tripleDELTA-cutL1 cells under anaerobic conditions. Mutant phenotypes, overview. Analysis of the monosaccharide composition of the cell walls from wild-type, tripleDELTA (agsADELTAagsBDELTAagsCDELTA), and quintupleDELTA (agsADELTAagsBDELTAagsCDELTAamyGDELTA), lyophilized hyphal cells of each strain of Aspergillus oryzae, overview. The HW fraction mainly contains galactose and mannose and the AS1 fraction mainly contains glucose and mannose. The alkali-soluble 2 (AS2) fraction derived from Aspergillus oryzae wild-type mainly contains glucose (approx. 20% of the AS2 fraction) with a small amount of mannose. But those derived from Aspergillus oryzae tripleDELTA and quintupleDELTA contain significantly less glucose. The alkali-soluble 1 (A1) fraction derived from each strain contains glucose, glucosamine, and mannose. The glucose and glucosamine contents of the A1 fraction derived from each strain are around 28% and 20% of the total dry weight of the A1 fraction, respectively
additional information
disruption of the genes encoding cell wall alpha-1,3-glucan synthases, i.e. agsA, agsB, agsC, in Aspergillus oryzae strain NS4 (sC-, niaD-) leads to increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae. Transformation of the cutinase-encoding gene cutL1 into Aspergillus oryzae wild-type and the tripleDELTA mutant showing that tripleDELTA-cutL1 forms smaller hyphal pellets and shows both greater biomass and increased CutL1 productivity compared to wild-type-cutL1, which might be attributable to a decrease in the number of tripleDELTA-cutL1 cells under anaerobic conditions. Mutant phenotypes, overview. Analysis of the monosaccharide composition of the cell walls from wild-type, tripleDELTA (agsADELTAagsBDELTAagsCDELTA), and quintupleDELTA (agsADELTAagsBDELTAagsCDELTAamyGDELTA), lyophilized hyphal cells of each strain of Aspergillus oryzae, overview. The HW fraction mainly contains galactose and mannose and the AS1 fraction mainly contains glucose and mannose. The alkali-soluble 2 (AS2) fraction derived from Aspergillus oryzae wild-type mainly contains glucose (approx. 20% of the AS2 fraction) with a small amount of mannose. But those derived from Aspergillus oryzae tripleDELTA and quintupleDELTA contain significantly less glucose. The alkali-soluble 1 (A1) fraction derived from each strain contains glucose, glucosamine, and mannose. The glucose and glucosamine contents of the A1 fraction derived from each strain are around 28% and 20% of the total dry weight of the A1 fraction, respectively
additional information
disruption of the genes encoding cell wall alpha-1,3-glucan synthases, i.e. agsA, agsB, agsC, in Aspergillus oryzae strain NS4 (sC-, niaD-) leads to increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae. Transformation of the cutinase-encoding gene cutL1 into Aspergillus oryzae wild-type and the tripleDELTA mutant showing that tripleDELTA-cutL1 forms smaller hyphal pellets and shows both greater biomass and increased CutL1 productivity compared to wild-type-cutL1, which might be attributable to a decrease in the number of tripleDELTA-cutL1 cells under anaerobic conditions. The amount of CutL1 secreted by the tripleDELTA-cutL1 and quintupleDELTA- cutL1 strains is approximately twice that produced by the wild-type-cutL1 strain. Analysis of the monosaccharide composition of the cell walls from wild-type, tripleDELTA (agsADELTAagsBDELTAagsCDELTA), and quintupleDELTA (agsADELTAagsBDELTAagsCDELTAamyGDELTA), lyophilized hyphal cells of each strain of Aspergillus oryzae, overview. The HW fraction mainly contains galactose and mannose and the AS1 fraction mainly contains glucose and mannose. The alkali-soluble 2 (AS2) fraction derived from Aspergillus oryzae wild-type mainly contains glucose (approx. 20% of the AS2 fraction) with a small amount of mannose. But those derived from Aspergillus oryzae tripleDELTA and quintupleDELTA contain significantly less glucose. The alkali-soluble 1 (A1) fraction derived from each strain contains glucose, glucosamine, and mannose. The glucose and glucosamine contents of the A1 fraction derived from each strain are around 28% and 20% of the total dry weight of the A1 fraction, respectively
additional information
disruption of the genes encoding cell wall alpha-1,3-glucan synthases, i.e. agsA, agsB, agsC, in Aspergillus oryzae strain NS4 (sC-, niaD-) leads to increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae. Transformation of the cutinase-encoding gene cutL1 into Aspergillus oryzae wild-type and the tripleDELTA mutant showing that tripleDELTA-cutL1 forms smaller hyphal pellets and shows both greater biomass and increased CutL1 productivity compared to wild-type-cutL1, which might be attributable to a decrease in the number of tripleDELTA-cutL1 cells under anaerobic conditions. The amount of CutL1 secreted by the tripleDELTA-cutL1 and quintupleDELTA- cutL1 strains is approximately twice that produced by the wild-type-cutL1 strain. Analysis of the monosaccharide composition of the cell walls from wild-type, tripleDELTA (agsADELTAagsBDELTAagsCDELTA), and quintupleDELTA (agsADELTAagsBDELTAagsCDELTAamyGDELTA), lyophilized hyphal cells of each strain of Aspergillus oryzae, overview. The HW fraction mainly contains galactose and mannose and the AS1 fraction mainly contains glucose and mannose. The alkali-soluble 2 (AS2) fraction derived from Aspergillus oryzae wild-type mainly contains glucose (approx. 20% of the AS2 fraction) with a small amount of mannose. But those derived from Aspergillus oryzae tripleDELTA and quintupleDELTA contain significantly less glucose. The alkali-soluble 1 (A1) fraction derived from each strain contains glucose, glucosamine, and mannose. The glucose and glucosamine contents of the A1 fraction derived from each strain are around 28% and 20% of the total dry weight of the A1 fraction, respectively
additional information
disruption of the genes encoding cell wall alpha-1,3-glucan synthases, i.e. agsA, agsB, agsC, in Aspergillus oryzae strain NS4 (sC-, niaD-) leads to increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae. Transformation of the cutinase-encoding gene cutL1 into Aspergillus oryzae wild-type and the tripleDELTA mutant showing that tripleDELTA-cutL1 forms smaller hyphal pellets and shows both greater biomass and increased CutL1 productivity compared to wild-type-cutL1, which might be attributable to a decrease in the number of tripleDELTA-cutL1 cells under anaerobic conditions. The amount of CutL1 secreted by the tripleDELTA-cutL1 and quintupleDELTA- cutL1 strains is approximately twice that produced by the wild-type-cutL1 strain. Analysis of the monosaccharide composition of the cell walls from wild-type, tripleDELTA (agsADELTAagsBDELTAagsCDELTA), and quintupleDELTA (agsADELTAagsBDELTAagsCDELTAamyGDELTA), lyophilized hyphal cells of each strain of Aspergillus oryzae, overview. The HW fraction mainly contains galactose and mannose and the AS1 fraction mainly contains glucose and mannose. The alkali-soluble 2 (AS2) fraction derived from Aspergillus oryzae wild-type mainly contains glucose (approx. 20% of the AS2 fraction) with a small amount of mannose. But those derived from Aspergillus oryzae tripleDELTA and quintupleDELTA contain significantly less glucose. The alkali-soluble 1 (A1) fraction derived from each strain contains glucose, glucosamine, and mannose. The glucose and glucosamine contents of the A1 fraction derived from each strain are around 28% and 20% of the total dry weight of the A1 fraction, respectively
additional information
disruption of the genes encoding cell wall alpha-1,3-glucan synthases, i.e. agsA, agsB, agsC, in Aspergillus oryzae strain NS4 (sC-, niaD-) leads to increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae. Transformation of the cutinase-encoding gene cutL1 into Aspergillus oryzae wild-type and the tripleDELTA mutant showing that tripleDELTA-cutL1 forms smaller hyphal pellets and shows both greater biomass and increased CutL1 productivity compared to wild-type-cutL1, which might be attributable to a decrease in the number of tripleDELTA-cutL1 cells under anaerobic conditions. Mutant phenotypes, overview. The amount of CutL1 secreted by the tripleDELTA-cutL1 and quintupleDELTA- cutL1 strains is approximately twice that produced by the wild-type-cutL1 strain. Analysis of the monosaccharide composition of the cell walls from wild-type, tripleDELTA (agsADELTAagsBDELTAagsCDELTA), and quintupleDELTA (agsADELTAagsBDELTAagsCDELTAamyGDELTA), lyophilized hyphal cells of each strain of Aspergillus oryzae, overview. The HW fraction mainly contains galactose and mannose and the AS1 fraction mainly contains glucose and mannose. The alkali-soluble 2 (AS2) fraction derived from Aspergillus oryzae wild-type mainly contains glucose (approx. 20% of the AS2 fraction) with a small amount of mannose. But those derived from Aspergillus oryzae tripleDELTA and quintupleDELTA contain significantly less glucose. The alkali-soluble 1 (A1) fraction derived from each strain contains glucose, glucosamine, and mannose. The glucose and glucosamine contents of the A1 fraction derived from each strain are around 28% and 20% of the total dry weight of the A1 fraction, respectively
additional information
disruption of the genes encoding cell wall alpha-1,3-glucan synthases, i.e. agsA, agsB, agsC, in Aspergillus oryzae strain NS4 (sC-, niaD-) leads to increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae. Transformation of the cutinase-encoding gene cutL1 into Aspergillus oryzae wild-type and the tripleDELTA mutant showing that tripleDELTA-cutL1 forms smaller hyphal pellets and shows both greater biomass and increased CutL1 productivity compared to wild-type-cutL1, which might be attributable to a decrease in the number of tripleDELTA-cutL1 cells under anaerobic conditions. Mutant phenotypes, overview. The amount of CutL1 secreted by the tripleDELTA-cutL1 and quintupleDELTA- cutL1 strains is approximately twice that produced by the wild-type-cutL1 strain. Analysis of the monosaccharide composition of the cell walls from wild-type, tripleDELTA (agsADELTAagsBDELTAagsCDELTA), and quintupleDELTA (agsADELTAagsBDELTAagsCDELTAamyGDELTA), lyophilized hyphal cells of each strain of Aspergillus oryzae, overview. The HW fraction mainly contains galactose and mannose and the AS1 fraction mainly contains glucose and mannose. The alkali-soluble 2 (AS2) fraction derived from Aspergillus oryzae wild-type mainly contains glucose (approx. 20% of the AS2 fraction) with a small amount of mannose. But those derived from Aspergillus oryzae tripleDELTA and quintupleDELTA contain significantly less glucose. The alkali-soluble 1 (A1) fraction derived from each strain contains glucose, glucosamine, and mannose. The glucose and glucosamine contents of the A1 fraction derived from each strain are around 28% and 20% of the total dry weight of the A1 fraction, respectively
additional information
disruption of the genes encoding cell wall alpha-1,3-glucan synthases, i.e. agsA, agsB, agsC, in Aspergillus oryzae strain NS4 (sC-, niaD-) leads to increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae. Transformation of the cutinase-encoding gene cutL1 into Aspergillus oryzae wild-type and the tripleDELTA mutant showing that tripleDELTA-cutL1 forms smaller hyphal pellets and shows both greater biomass and increased CutL1 productivity compared to wild-type-cutL1, which might be attributable to a decrease in the number of tripleDELTA-cutL1 cells under anaerobic conditions. Mutant phenotypes, overview. The amount of CutL1 secreted by the tripleDELTA-cutL1 and quintupleDELTA- cutL1 strains is approximately twice that produced by the wild-type-cutL1 strain. Analysis of the monosaccharide composition of the cell walls from wild-type, tripleDELTA (agsADELTAagsBDELTAagsCDELTA), and quintupleDELTA (agsADELTAagsBDELTAagsCDELTAamyGDELTA), lyophilized hyphal cells of each strain of Aspergillus oryzae, overview. The HW fraction mainly contains galactose and mannose and the AS1 fraction mainly contains glucose and mannose. The alkali-soluble 2 (AS2) fraction derived from Aspergillus oryzae wild-type mainly contains glucose (approx. 20% of the AS2 fraction) with a small amount of mannose. But those derived from Aspergillus oryzae tripleDELTA and quintupleDELTA contain significantly less glucose. The alkali-soluble 1 (A1) fraction derived from each strain contains glucose, glucosamine, and mannose. The glucose and glucosamine contents of the A1 fraction derived from each strain are around 28% and 20% of the total dry weight of the A1 fraction, respectively
additional information
-
disruption of the genes encoding cell wall alpha-1,3-glucan synthases, i.e. agsA, agsB, agsC, in Aspergillus oryzae strain NS4 (sC-, niaD-) leads to increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae. Transformation of the cutinase-encoding gene cutL1 into Aspergillus oryzae wild-type and the tripleDELTA mutant showing that tripleDELTA-cutL1 forms smaller hyphal pellets and shows both greater biomass and increased CutL1 productivity compared to wild-type-cutL1, which might be attributable to a decrease in the number of tripleDELTA-cutL1 cells under anaerobic conditions. Mutant phenotypes, overview. Analysis of the monosaccharide composition of the cell walls from wild-type, tripleDELTA (agsADELTAagsBDELTAagsCDELTA), and quintupleDELTA (agsADELTAagsBDELTAagsCDELTAamyGDELTA), lyophilized hyphal cells of each strain of Aspergillus oryzae, overview. The HW fraction mainly contains galactose and mannose and the AS1 fraction mainly contains glucose and mannose. The alkali-soluble 2 (AS2) fraction derived from Aspergillus oryzae wild-type mainly contains glucose (approx. 20% of the AS2 fraction) with a small amount of mannose. But those derived from Aspergillus oryzae tripleDELTA and quintupleDELTA contain significantly less glucose. The alkali-soluble 1 (A1) fraction derived from each strain contains glucose, glucosamine, and mannose. The glucose and glucosamine contents of the A1 fraction derived from each strain are around 28% and 20% of the total dry weight of the A1 fraction, respectively
-
additional information
-
disruption of the genes encoding cell wall alpha-1,3-glucan synthases, i.e. agsA, agsB, agsC, in Aspergillus oryzae strain NS4 (sC-, niaD-) leads to increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae. Transformation of the cutinase-encoding gene cutL1 into Aspergillus oryzae wild-type and the tripleDELTA mutant showing that tripleDELTA-cutL1 forms smaller hyphal pellets and shows both greater biomass and increased CutL1 productivity compared to wild-type-cutL1, which might be attributable to a decrease in the number of tripleDELTA-cutL1 cells under anaerobic conditions. The amount of CutL1 secreted by the tripleDELTA-cutL1 and quintupleDELTA- cutL1 strains is approximately twice that produced by the wild-type-cutL1 strain. Analysis of the monosaccharide composition of the cell walls from wild-type, tripleDELTA (agsADELTAagsBDELTAagsCDELTA), and quintupleDELTA (agsADELTAagsBDELTAagsCDELTAamyGDELTA), lyophilized hyphal cells of each strain of Aspergillus oryzae, overview. The HW fraction mainly contains galactose and mannose and the AS1 fraction mainly contains glucose and mannose. The alkali-soluble 2 (AS2) fraction derived from Aspergillus oryzae wild-type mainly contains glucose (approx. 20% of the AS2 fraction) with a small amount of mannose. But those derived from Aspergillus oryzae tripleDELTA and quintupleDELTA contain significantly less glucose. The alkali-soluble 1 (A1) fraction derived from each strain contains glucose, glucosamine, and mannose. The glucose and glucosamine contents of the A1 fraction derived from each strain are around 28% and 20% of the total dry weight of the A1 fraction, respectively
-
additional information
-
disruption of the genes encoding cell wall alpha-1,3-glucan synthases, i.e. agsA, agsB, agsC, in Aspergillus oryzae strain NS4 (sC-, niaD-) leads to increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae. Transformation of the cutinase-encoding gene cutL1 into Aspergillus oryzae wild-type and the tripleDELTA mutant showing that tripleDELTA-cutL1 forms smaller hyphal pellets and shows both greater biomass and increased CutL1 productivity compared to wild-type-cutL1, which might be attributable to a decrease in the number of tripleDELTA-cutL1 cells under anaerobic conditions. Mutant phenotypes, overview. The amount of CutL1 secreted by the tripleDELTA-cutL1 and quintupleDELTA- cutL1 strains is approximately twice that produced by the wild-type-cutL1 strain. Analysis of the monosaccharide composition of the cell walls from wild-type, tripleDELTA (agsADELTAagsBDELTAagsCDELTA), and quintupleDELTA (agsADELTAagsBDELTAagsCDELTAamyGDELTA), lyophilized hyphal cells of each strain of Aspergillus oryzae, overview. The HW fraction mainly contains galactose and mannose and the AS1 fraction mainly contains glucose and mannose. The alkali-soluble 2 (AS2) fraction derived from Aspergillus oryzae wild-type mainly contains glucose (approx. 20% of the AS2 fraction) with a small amount of mannose. But those derived from Aspergillus oryzae tripleDELTA and quintupleDELTA contain significantly less glucose. The alkali-soluble 1 (A1) fraction derived from each strain contains glucose, glucosamine, and mannose. The glucose and glucosamine contents of the A1 fraction derived from each strain are around 28% and 20% of the total dry weight of the A1 fraction, respectively
-
additional information
-
disruption of the genes encoding cell wall alpha-1,3-glucan synthases, i.e. agsA, agsB, agsC, in Aspergillus oryzae strain NS4 (sC-, niaD-) leads to increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae. Transformation of the cutinase-encoding gene cutL1 into Aspergillus oryzae wild-type and the tripleDELTA mutant showing that tripleDELTA-cutL1 forms smaller hyphal pellets and shows both greater biomass and increased CutL1 productivity compared to wild-type-cutL1, which might be attributable to a decrease in the number of tripleDELTA-cutL1 cells under anaerobic conditions. Mutant phenotypes, overview. Analysis of the monosaccharide composition of the cell walls from wild-type, tripleDELTA (agsADELTAagsBDELTAagsCDELTA), and quintupleDELTA (agsADELTAagsBDELTAagsCDELTAamyGDELTA), lyophilized hyphal cells of each strain of Aspergillus oryzae, overview. The HW fraction mainly contains galactose and mannose and the AS1 fraction mainly contains glucose and mannose. The alkali-soluble 2 (AS2) fraction derived from Aspergillus oryzae wild-type mainly contains glucose (approx. 20% of the AS2 fraction) with a small amount of mannose. But those derived from Aspergillus oryzae tripleDELTA and quintupleDELTA contain significantly less glucose. The alkali-soluble 1 (A1) fraction derived from each strain contains glucose, glucosamine, and mannose. The glucose and glucosamine contents of the A1 fraction derived from each strain are around 28% and 20% of the total dry weight of the A1 fraction, respectively
-
additional information
-
disruption of the genes encoding cell wall alpha-1,3-glucan synthases, i.e. agsA, agsB, agsC, in Aspergillus oryzae strain NS4 (sC-, niaD-) leads to increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae. Transformation of the cutinase-encoding gene cutL1 into Aspergillus oryzae wild-type and the tripleDELTA mutant showing that tripleDELTA-cutL1 forms smaller hyphal pellets and shows both greater biomass and increased CutL1 productivity compared to wild-type-cutL1, which might be attributable to a decrease in the number of tripleDELTA-cutL1 cells under anaerobic conditions. The amount of CutL1 secreted by the tripleDELTA-cutL1 and quintupleDELTA- cutL1 strains is approximately twice that produced by the wild-type-cutL1 strain. Analysis of the monosaccharide composition of the cell walls from wild-type, tripleDELTA (agsADELTAagsBDELTAagsCDELTA), and quintupleDELTA (agsADELTAagsBDELTAagsCDELTAamyGDELTA), lyophilized hyphal cells of each strain of Aspergillus oryzae, overview. The HW fraction mainly contains galactose and mannose and the AS1 fraction mainly contains glucose and mannose. The alkali-soluble 2 (AS2) fraction derived from Aspergillus oryzae wild-type mainly contains glucose (approx. 20% of the AS2 fraction) with a small amount of mannose. But those derived from Aspergillus oryzae tripleDELTA and quintupleDELTA contain significantly less glucose. The alkali-soluble 1 (A1) fraction derived from each strain contains glucose, glucosamine, and mannose. The glucose and glucosamine contents of the A1 fraction derived from each strain are around 28% and 20% of the total dry weight of the A1 fraction, respectively
-
additional information
-
disruption of the genes encoding cell wall alpha-1,3-glucan synthases, i.e. agsA, agsB, agsC, in Aspergillus oryzae strain NS4 (sC-, niaD-) leads to increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae. Transformation of the cutinase-encoding gene cutL1 into Aspergillus oryzae wild-type and the tripleDELTA mutant showing that tripleDELTA-cutL1 forms smaller hyphal pellets and shows both greater biomass and increased CutL1 productivity compared to wild-type-cutL1, which might be attributable to a decrease in the number of tripleDELTA-cutL1 cells under anaerobic conditions. Mutant phenotypes, overview. The amount of CutL1 secreted by the tripleDELTA-cutL1 and quintupleDELTA- cutL1 strains is approximately twice that produced by the wild-type-cutL1 strain. Analysis of the monosaccharide composition of the cell walls from wild-type, tripleDELTA (agsADELTAagsBDELTAagsCDELTA), and quintupleDELTA (agsADELTAagsBDELTAagsCDELTAamyGDELTA), lyophilized hyphal cells of each strain of Aspergillus oryzae, overview. The HW fraction mainly contains galactose and mannose and the AS1 fraction mainly contains glucose and mannose. The alkali-soluble 2 (AS2) fraction derived from Aspergillus oryzae wild-type mainly contains glucose (approx. 20% of the AS2 fraction) with a small amount of mannose. But those derived from Aspergillus oryzae tripleDELTA and quintupleDELTA contain significantly less glucose. The alkali-soluble 1 (A1) fraction derived from each strain contains glucose, glucosamine, and mannose. The glucose and glucosamine contents of the A1 fraction derived from each strain are around 28% and 20% of the total dry weight of the A1 fraction, respectively
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
protoplast generation in Aspergillus luchuensis wild-type and DELTAagsE strains
recombinant as His-tagged or GST-tagged proteins from Escherichia coli
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning of eps gene cluster from genomic library and expression in Escherichia coli as His-tagges or GST-tagged proteins, DNA sequence analysis
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expression in Escherichia coli. Isolation of fragments of a family of five 1,3-alpha D-glucan synthase-encoding genes (agsA-E)
gene agsA, recombinant expression of GFP-tagged enzyme in Aspergillus nidulans
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gene agsB, quantitative PCR enzyme expression analysis, constitutive overexpression in Aspergillus nidulans. The high level of agsB expression is deleterious
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gene agsB, recombinant expression of GFP-tagged enzyme in Aspergillus nidulans
gene agsB, recombinant expression of GFP-tagged enzyme in Aspergillus nidulans
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gene agsB, recombinant expression of GFP-tagged enzyme in Aspergillus nidulans
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
AgsE gene is highly expressed during the first two hours of germination and vegetative growth when exposed to caspofungin as well as under non-perturbed growth conditions with fructose, galactose, glucose, and mannose
in presence of inulin, maltose, and xylose, expression is increased after 8 h
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
synthesis
synthesis
in a strain producing free dihomo-gamma-linolenic acid at an increased yield by faaA gene disruption and cooverexpression of one elongase and two desaturase genes, disruption of gene agsB further increases yield by 1.3-fold to 533 mg/l
synthesis
-
in a strain producing free dihomo-gamma-linolenic acid at an increased yield by faaA gene disruption and cooverexpression of one elongase and two desaturase genes, disruption of gene agsB further increases yield by 1.3-fold to 533 mg/l
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Yamashita, Y.; Hanada, N.; Itoh-Andoh, M.; Takehara, T.
Evidence for the presence of two distinct sites of sucrose hydrolysis and glucosyl transfer activities on 1,3-alpha-D-glucan synthase of Streptococcus mutans
FEBS Lett.
243
343-346
1989
Streptococcus mutans, Streptococcus mutans AHT
brenda
Jolly, L.; Newell, J.; Porcelli, I.; Vincent, S.J.F.; Stingele, F.
Lactobacillus helveticus glycosyltransferases: from genes to carbohydrate synthesis
Glycobiology
12
319-327
2002
Lactobacillus helveticus, Lactobacillus helveticus NCC2745
brenda
Damveld, R.A.; van Kuyk, P.A.; Arentshorst, M.; Klis, F.M.; van den Hondel, C.A.M.J.J.; Ram, A.F.J.
Expression of agsA, one of five 1,3-alpha-D-glucan synthase-encoding genes in Aspergillus niger, is induced in response to cell wall stress
Fungal Genet. Biol.
42
165-177
2005
Aspergillus niger (Q5GHR7), Aspergillus niger (Q5GHR6), Aspergillus niger (Q5GHS0), Aspergillus niger (Q5GHR9), Aspergillus niger N402 (Q5GHR7), Aspergillus niger N402 (Q5GHR6), Aspergillus niger N402 (Q5GHS0), Aspergillus niger N402 (Q5GHR9), Penicillium chrysogenum (Q5GHR5), Penicillium chrysogenum (Q5GHR4), Penicillium chrysogenum (Q5GHR3)
brenda
Maubon, D.; Park, S.; Tanguy, M.; Huerre, M.; Schmitt, C.; Prevost, M.C.; Perlin, D.S.; Latge, J.P.; Beauvais, A.
AGS3, an a(1-3)glucan synthase gene family member of Aspergillus fumigatus, modulates mycelium growth in the lung of experimentally infected mice
Fungal Genet. Biol.
43
366-375
2006
Aspergillus fumigatus
brenda
Damveld, R.A.; Arentshorst, M.; Franken, A.; vanKuyk, P.A.; Klis, F.M.; van den Hondel, C.A.; Ram, A.F.
The Aspergillus niger MADS-box transcription factor RlmA is required for cell wall reinforcement in response to cell wall stress
Mol. Microbiol.
58
305-319
2005
Aspergillus niger, Aspergillus niger N402
brenda
Yoshimi, A.; Sano, M.; Inaba, A.; Kokubun, Y.; Fujioka, T.; Mizutani, O.; Hagiwara, D.; Fujikawa, T.; Nishimura, M.; Yano, S.; Kasahara, S.; Shimizu, K.; Yamaguchi, M.; Kawakami, K.; Abe, K.
Functional analysis of the alpha-1,3-glucan synthase genes agsA and agsB in Aspergillus nidulans: agsB is the major alpha-1,3-glucan synthase in this fungus
PLoS ONE
8
e54893
2013
Aspergillus nidulans (Q5B0P5), Aspergillus nidulans ATCC 38163 (Q5B0P5)
brenda
Beauvais, A.; Bozza, S.; Kniemeyer, O.; Formosa, C.; Formosa, C.; Balloy, V.; Henry, C.; Roberson, R.W.; Dague, E.; Chignard, M.; Brakhage, A.A.; Romani, L.; Latge, J.P.
Deletion of the alpha-(1,3)-glucan synthase genes induces a restructuring of the conidial cell wall responsible for the avirulence of Aspergillus fumigatus
PLoS Pathog.
9
e1003716
2013
Aspergillus fumigatus (Q4WFV3), Aspergillus fumigatus (Q4X143), Aspergillus fumigatus (Q4WRQ8)
brenda
Miyazawa, K.; Yoshimi, A.; Zhang, S.; Sano, M.; Nakayama, M.; Gomi, K.; Abe, K.
Increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae by disruption of the genes encoding cell wall alpha-1,3-glucan synthase
Biosci. Biotechnol. Biochem.
80
1853-1863
2016
Aspergillus oryzae (Q2TXK6), Aspergillus oryzae (Q2UEQ6), Aspergillus oryzae (Q2UIS4), Aspergillus oryzae ATCC 42149 (Q2TXK6), Aspergillus oryzae ATCC 42149 (Q2UEQ6), Aspergillus oryzae ATCC 42149 (Q2UIS4), Aspergillus oryzae RIB 40 (Q2TXK6), Aspergillus oryzae RIB 40 (Q2UEQ6), Aspergillus oryzae RIB 40 (Q2UIS4)
brenda
Miyazawa, K.; Yoshimi, A.; Kasahara, S.; Sugahara, A.; Koizumi, A.; Yano, S.; Kimura, S.; Iwata, T.; Sano, M.; Abe, K.
Molecular mass and localization of alpha-1,3-glucan in cell wall control the degree of hyphal aggregation in liquid culture of Aspergillus nidulans
Front. Microbiol.
9
2623
2018
Aspergillus nidulans, Aspergillus nidulans ABPU1, Aspergillus oryzae
brenda
Tokashiki, J.; Hayashi, R.; Yano, S.; Watanabe, T.; Yamada, O.; Toyama, H.; Mizutani, O.
Influence of alpha-1,3-glucan synthase gene agsE on protoplast formation for transformation of Aspergillus luchuensis
J. Biosci. Bioeng.
128
129-134
2019
Aspergillus luchuensis (A0A146F7N5)
brenda
He, X.; Li, S.; Kaminskyj, S.G.W.
Overexpression of Aspergillus nidulans alpha-1,3-glucan synthase increases cellular adhesion and causes cell wall defects
Med. Mycol.
56
645-648
2018
Aspergillus nidulans
brenda
Lyu, J.; Torchia, C.; Post, H.; Moran Torres, J.P.; Altelaar, A.F.M.; de Cock, H.; Woesten, H.A.B.
The alpha-(1,3)-glucan synthase gene agsE impacts the secretome of Aspergillus niger
Antonie van Leeuwenhoek
116
867-882
2023
Aspergillus niger (Q5GHR6)
brenda
Tamano, K.; Nakai, S.; Takayama, H.; Imai, Y.
Overexpression of a predicted transketolase gene and disruption of an alpha-1,3-glucan synthase gene in Aspergillus oryzae DGLA3 strain enhances the yield of free dihomo-gamma-linolenic acid
Biosci. Biotechnol. Biochem.
87
448-457
2023
Aspergillus oryzae (Q2UIS4), Aspergillus oryzae ATCC 42149 (Q2UIS4)
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brenda
Ost, K.J.; Arentshorst, M.; Moerschbacher, B.M.; Dirks-Hofmeister, M.E.; Ram, A.F.J.
Comprehensive phenotypic analysis of multiple gene deletions of alpha-glucan synthase and Crh-transglycosylase gene families in Aspergillus niger highlighting the versatility of the fungal cell wall
Cell Surf.
13
100141
2025
Aspergillus niger (Q5GHR6)
brenda
Ding, C.; Zheng, Y.; Wang, X.; Gao, K.; Sun, Z.; Wang, N.; Chen, M.; Wang, C.; Guo, Q.
Structural and immunomodulatory changes in mycelial and exopolysaccharides from Monascus purpureus M9 after mutation of the alpha-1,3-glucan synthase gene
J. Agric. Food Chem.
73
12038-12048
2025
Monascus purpureus, Monascus purpureus M9
brenda
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