Information on EC 3.2.1.108 - lactase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY
3.2.1.108
-
RECOMMENDED NAME
GeneOntology No.
lactase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
lactose + H2O = D-galactose + D-glucose
show the reaction diagram
mechanism
-
lactose + H2O = D-galactose + D-glucose
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis
-
-
hydrolysis
-
-
hydrolysis of O-glycosyl bond
-
-
-
-
hydrolysis of O-glycosyl bond
-
-
hydrolysis of O-glycosyl bond
-
-
hydrolysis of O-glycosyl bond
-
-
hydrolysis of O-glycosyl bond
Mus musculus BALB/c
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Galactose metabolism
-
Metabolic pathways
-
SYSTEMATIC NAME
IUBMB Comments
lactose galactohydrolase
The enzyme from intestinal mucosa is isolated as a complex that also catalyses the reaction of EC 3.2.1.62 glycosylceramidase. cf. EC 3.2.1.33 amylo-alpha-1,6-glucosidase.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
intestinal lactase
-
-
lactase
P09848
-
lactase
-
-
lactase
Mus musculus BALB/c
-
-
-
lactase phlorizin hydrolase
-
-
lactase phlorizin hydrolase
-
-
lactase phlorizin hydrolase
Q29587
-
lactase-phlorizin hydrolase
-
-
-
-
lactase-phlorizin hydrolase
-
-
lactase-phlorizin hydrolase
-
-
lactase-phlorizin hydrolase
Mus musculus BALB/c
-
-
-
lactase-phlorizin hydrolase
-
-
lactase-phlorizin hydrolase LPH
-
-
lactase-phlorozin hydrolase
-
-
lactase/phlorizin hydrolase
-
-
-
-
LPH
Mus musculus BALB/c
-
-
-
LPH
Q29587
-
CAS REGISTRY NUMBER
COMMENTARY
9031-11-2
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
BALB/c mouse
-
-
Manually annotated by BRENDA team
Mus musculus BALB/c
BALB/c mouse
-
-
Manually annotated by BRENDA team
lactase-phlorizin hydrolase complex
-
-
Manually annotated by BRENDA team
Sprague-Dawley rat
-
-
Manually annotated by BRENDA team
food supplemented with insulin-like growth factor I
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-O-beta-D-galactopyranosyl-D-xylose + H2O
D-galactose + D-xylose
show the reaction diagram
-
-
-
-
?
3-O-beta-D-galactopyranosyl-D-xylose + H2O
D-galactose + D-xylose
show the reaction diagram
-
-
-
-
?
4-O-beta-D-galactopyranosyl-D-xylose + H2O
D-galactose + D-xylose
show the reaction diagram
-
-
-
-
?
5-bromo-4-chloro-3-indolyl-beta-D-fucopyranoside + H2O
?
show the reaction diagram
-
37C, 1 hour, full-length lactase, mature lactase, cytosolic form of mature lactase
-
-
?
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O
?
show the reaction diagram
-
37C, 1 h
-
-
?
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O
?
show the reaction diagram
-
37C, 1 hour, only cytosolic form of mature lactase
-
-
?
cellobiose + H2O
2 beta-D-glucose
show the reaction diagram
-
16% of the activity with lactose
-
-
?
cellobiose + H2O
2 beta-D-glucose
show the reaction diagram
-
25% of the activity with lactose
-
-
?
cellobiose + H2O
2 beta-D-glucose
show the reaction diagram
-
14% of the activity with lactose
-
-
?
cellotetraose + 3 H2O
4 beta-D-glucose
show the reaction diagram
-
14% of the activity with lactose
-
-
?
cellotetraose + 3 H2O
4 beta-D-glucose
show the reaction diagram
-
9% of the activity with lactose
-
-
?
cellotriose + H2O
beta-D-glucose
show the reaction diagram
-
14% of the activity with lactose
-
-
?
cellotriose + H2O
beta-D-glucose
show the reaction diagram
-
9% of the activity with lactose
-
-
?
cellulose + H2O
beta-D-glucose
show the reaction diagram
-
-
-
-
?
cellulose + H2O
beta-D-glucose
show the reaction diagram
-
0.1% of the activity with lactose
-
-
?
daidzein 7-glucoside + H2O
quercetin + D-glucose
show the reaction diagram
-
-
-
-
?
daidzein-7-glucoside + H2O
quercetin + D-glucose
show the reaction diagram
-
-
-
-
?
fluorescein-di-beta-D-galactopyranoside + H2O
?
show the reaction diagram
-
only cytosolic form of mature lactase
-
-
?
genistein-7-glucoside + H2O
genistein + D-glucose
show the reaction diagram
-
-
-
-
?
gentiobiose + H2O
2 beta-D-glucose
show the reaction diagram
-
0.7% of the activity with lactose
-
-
?
lactose + H2O
D-glucose + D-galactose
show the reaction diagram
-
-
-
?
lactose + H2O
D-glucose + D-galactose
show the reaction diagram
-
-
-
?
lactose + H2O
D-glucose + D-galactose
show the reaction diagram
-
-
-
?
lactose + H2O
D-glucose + D-galactose
show the reaction diagram
-
-
-
-
?
lactose + H2O
D-glucose + D-galactose
show the reaction diagram
-
-
-
?
lactose + H2O
D-glucose + D-galactose
show the reaction diagram
-
-
-
?
lactose + H2O
D-glucose + D-galactose
show the reaction diagram
-
-
-
-
?
lactose + H2O
D-glucose + D-galactose
show the reaction diagram
-
-
-
?
lactose + H2O
D-glucose + D-galactose
show the reaction diagram
Q29587
-
-
-
?
lactose + H2O
D-glucose + D-galactose
show the reaction diagram
-
-
most active substrate
?
lactose + H2O
D-glucose + D-galactose
show the reaction diagram
-
rotavirus RRV infection affects the lactose metabolism of enterocytes in the intestine, overview
-
-
?
lactose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
-
?
lactose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
-
?
lactose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
-
?
lactose + H2O
D-galactose + D-glucose
show the reaction diagram
-
in rats grown under iron deficiency lactase-phlorizin hydrolase activity was 56.8% lower compared to control rats
-
-
?
lactose + H2O
D-galactose + D-glucose
show the reaction diagram
-
the nuclear transcription factor Oct-1 binds to the T variant at position -13910 upstream of the lactase gene and enhances transcription of the lactase gene
-
-
?
lactose + H2O
D-galactose + D-glucose
show the reaction diagram
-
1h, 37C, pH 6.0
-
-
?
lactose + H2O
D-galactose + D-glucose
show the reaction diagram
-
37C, 2h
-
-
?
lactose + H2O
D-galactose + D-glucose
show the reaction diagram
Mus musculus BALB/c
-
-
-
-
?
lactose + H2O
galactose + D-glucose
show the reaction diagram
-
reaction is not inhibited by 2-fluoro-dinotrophenylglucoside
-
-
?
o-nitrophenyl-beta-galactoside + H2O
o-nitrophenol + beta-D-galactose
show the reaction diagram
-
15.4% of the activity with lactose
-
-
?
o-nitrophenyl-beta-galactoside + H2O
o-nitrophenol + beta-D-galactose
show the reaction diagram
-
17% of the activity with lactose
-
-
?
o-nitrophenyl-beta-glucoside + H2O
o-nitrophenol + beta-D-glucose
show the reaction diagram
-
13% of the activity with lactose
-
-
?
p-nitrophenyl-beta-galactoside + H2O
p-nitrophenol + beta-D-galactose
show the reaction diagram
-
13% of the activity with lactose
-
-
?
p-nitrophenyl-beta-galactoside + H2O
p-nitrophenol + beta-D-galactose
show the reaction diagram
-
9.3% of the activity with lactose
-
-
?
p-nitrophenyl-beta-glucoside + H2O
p-nitrophenol + beta-D-glucose
show the reaction diagram
-
10% of the activity with lactose
-
-
?
p-nitrophenyl-beta-glucoside + H2O
p-nitrophenol + beta-D-glucose
show the reaction diagram
-
12.7% of the activity with lactose
-
-
?
phenyl-beta-galactoside + H2O
phenol + beta-D-galactose
show the reaction diagram
-
0.1% of the activity with lactose
-
-
?
phlorizin
phloretin + D-glucose
show the reaction diagram
-
37C, 2h
-
-
?
phlorizin + H2O
phloretin + D-glucose
show the reaction diagram
-
-
-
-
?
phlorizin + H2O
phloretin + D-glucose
show the reaction diagram
-
-
-
?
phlorizin + H2O
phloretin + D-glucose
show the reaction diagram
-
22% of the activity with lactose
-
-
?
phlorizin + H2O
phloretin + D-glucose
show the reaction diagram
-
at 2.3% of the activity with lactose
-
-
?
phlorizin + H2O
?
show the reaction diagram
-
reaction is inhibited by 2-fluoro-dinotrophenylglucoside
-
-
?
quercetin-3-glucoside + H2O
quercetin + D-glucose
show the reaction diagram
-
reaction is marginally inhibited by 2-fluoro-dinotrophenylglucoside
-
-
?
quercetin-4'-glucoside + H2O
quercetin + D-glucose
show the reaction diagram
-
reaction is marginally inhibited by 2-fluoro-dinotrophenylglucoside
-
-
?
salicin + H2O
?
show the reaction diagram
-
1.6% of the activity with lactose
-
-
?
methyl-beta-D-glucoside + H2O
methanol + beta-D-glucose
show the reaction diagram
-
7.3% of the activity with lactose
-
-
?
additional information
?
-
-
total lactase activity increases to reach maximal levels around weaning, and remains high subsequently, enzyme activity appears to be regulated during development predominantly by transcription mechanism, while alterations during lactation, and along the proximal to distal gradient, are the result of other control mechanism
-
-
-
additional information
?
-
-
absorption of quercetin-3-glucoside requires hydrolysis by the enzyme, absorption of quercetin-4-glucoside requires both an interaction with sodium-dependent glucose transporter and hydrolysis by the enzyme
-
-
-
additional information
?
-
-
enzyme plays a major role in metabolism of glycosylated phytochemicals
-
-
-
additional information
?
-
-
no substrate: rutin, naringin
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
lactose + H2O
D-glucose + D-galactose
show the reaction diagram
-
rotavirus RRV infection affects the lactose metabolism of enterocytes in the intestine, overview
-
-
?
lactose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
-
?
lactose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
-
?
lactose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
-
?
lactose + H2O
D-galactose + D-glucose
show the reaction diagram
-
in rats grown under iron deficiency lactase-phlorizin hydrolase activity was 56.8% lower compared to control rats
-
-
?
lactose + H2O
D-galactose + D-glucose
show the reaction diagram
-
the nuclear transcription factor Oct-1 binds to the T variant at position -13910 upstream of the lactase gene and enhances transcription of the lactase gene
-
-
?
lactose + H2O
D-galactose + D-glucose
show the reaction diagram
Mus musculus BALB/c
-
-
-
-
?
additional information
?
-
-
total lactase activity increases to reach maximal levels around weaning, and remains high subsequently, enzyme activity appears to be regulated during development predominantly by transcription mechanism, while alterations during lactation, and along the proximal to distal gradient, are the result of other control mechanism
-
-
-
additional information
?
-
-
absorption of quercetin-3-glucoside requires hydrolysis by the enzyme, absorption of quercetin-4-glucoside requires both an interaction with sodium-dependent glucose transporter and hydrolysis by the enzyme
-
-
-
additional information
?
-
-
enzyme plays a major role in metabolism of glycosylated phytochemicals
-
-
-
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2-fluoro-dinitrophenylglucoside
-
specifically inhibits phlorizin hydrolase, lactase activity is inhibited slowly
cerebroside
-
bovine brain cerebroside
D-glucal
-
40% inhibition for 4-O-beta-D-galactopyranosyl-D-xylose and 75% inhibition for 3-O-beta-D-galactopyranosyl-D-xylose at 100 mM D-glucal, no increase of inhibition above 100 mM D-glucal
N-(n-butyl)-deoxygalactojirimycin
-
-
NSP4
-
impairment of lactase activity observed during rotavirus infection results from an inhibitory action of the secreted non-structural rotavirus protein NSP4
-
phlorizin
-
competitive inhibitor of lactose hydrolysis
phlorizin
-
competitive inhibitor of lactose hydrolysis
thyroxine
-
administration of thyroxine to 8-day-old pups shows a decline in lactase activity
Tris
-
competitive
Tris
-
competitive
epigallocatechin-3-gallate
-
inhibition by epigallocatechin-3-gallate of lactase is alleviated about 1.7fold by proline-rich proteins
additional information
-
rotavirus impairs lactase enzymatic activity by an NSP4-dependant mechanism in brush border of human enterocyte-like Caco-2 cells, the infected brush border membranes show an enzyme with altered kinetics, overview
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
cortisone
-
administration of cortisone to 8-day-old pups augments lactase activity
additional information
-
determination of disaccharidase activities in intestinal biopsy samples show a highly significant correlation between lactase activity and the T/G-13915 genotype
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
22.2
-
2-O-beta-D-galactopyranosyl-D-xylose
-
100 mM sodium maleate buffer, pH 6.0, 25C
8.5
-
3-O-beta-D-galactopyranosyl-D-xylose
-
100 mM sodium maleate buffer, pH 6.0, 25C
58.8
-
4-O-beta-D-galactopyranosyl-D-xylose
-
100 mM sodium maleate buffer, pH 6.0, 25C
2.78
-
cellobiose
-
-
4.4
-
cellobiose
-
-
0.149
-
daidzein-7-glucoside
-
pH 6, 37C
0.085
-
genistein-7-glucoside
-
pH 6, 37C
5.1
-
lactose
-
-
8.8
-
lactose
-
pH 6, 37C
17.9
-
lactose
-
100 mM sodium malaete buffer, pH 6.0, 25C
21
-
lactose
-
-
21
-
lactose
-
-
21.8
-
lactose
-
-
27
-
lactose
-
100 mM maleate buffer pH 6.0, 37C
28.9
-
lactose
-
PPH from RRV-infected cells
0.002
-
phlorizin
-
pH 6, 37C
0.4
-
phlorizin
-
-
0.44
-
phlorizin
-
-
0.046
-
quercetin-3-glucoside
-
pH 6, 37C
0.044
-
quercetin-4'-glucoside
-
pH 6, 37C
30
-
lactose
-
LPH from uninfected cells
additional information
-
additional information
-
rotavirus infection decreases Vmax of LPH, overview
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2.1
-
daidzein-7-glucoside
-
pH 6, 37C
6.5
-
genistein-7-glucoside
-
pH 6, 37C
33.8
-
lactose
-
pH 6, 37C
0.5
-
phlorizin
-
pH 6, 37C
6.3
-
quercetin-3-glucoside
-
pH 6, 37C
7.5
-
quercetin-4'-glucoside
-
pH 6, 37C
IC50 VALUE [mM]
IC50 VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.074
-
epigallocatechin-3-gallate
-
in the absence of proline-rich protein fraction, in 1.5 mM sodium phosphate buffer (pH 6.8), at 37C
0.126
-
epigallocatechin-3-gallate
-
in the presence of proline-rich protein fraction, in 1.5 mM sodium phosphate buffer (pH 6.8), at 37C
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.00164
-
-
non-infected cells, lactase enzyme activity in cell homogenates
0.00167
-
-
rhesus monkey rotavirus-, RRV, infected cells, lactase enzyme activity in cell homogenates
0.002
-
-
mutant S218fsX224, mean lactase activity
0.0021
-
-
mutant Y1390X, mean lactase activity
0.00219
-
-
rhesus monkey rotavirus-, RRV, infected cells, lactase enzyme activity in brush border-enriched fraction
0.003
-
-
mutant G1363S, mean lactase activity
0.0033
-
-
rats are force-fed a low-strach diet, with thyroid hormone treatment
0.00344
-
-
non-infected cells, lactase enzyme activity in brush border-enriched fraction
0.00359
-
-
UV, psoralen-inactivated RRV infected cells, lactase enzyme activity in brush border-enriched fraction
0.0042
-
-
rats are fed a low-starch diet for 7 days
0.005
-
-
mutant Q268H, mean lactase activity; mutant S1666fsX1722, mean lactase activity
0.0058
-
-
rats are force-fed a sucrose diet, with thyroid hormone treatment
0.0067
-
-
rats are fed a low-starch diet, starved for 3 days, and thyroid hormones are injected daily after the diet
0.0083
-
-
rats are force-fed a low-strach diet, without thyroid hormone treatment; rats are force-fed a sucrose diet, without thyroid hormone treatment
0.01
-
-
rats are fed a high-starch diet for 7 days
0.012
-
-
rats are fed a low-starch diet, starved for 3 days
0.015
-
-
30 days old rat
0.07
-
-
18 days old rat
0.17
-
-
administration of thyroxine to 8-day-old pups, activity in suckling rat intestine
0.172
-
-
15 days old rat
0.27
-
-
control
0.29
-
-
administration of insuline to 8-day-old pups, activity in suckling rat intestine
0.36
-
-
administration of cortisone to 8-day-old pups, activity in suckling rat intestine
16.1
-
-
lactase
18
-
-
-
32
-
-
-
450
-
-
phlorizin hydrolase
additional information
-
-
20% enzyme activity of mutant G1363S after incubation at 20C for 22h; enzymatic activity of mutant G1363S below detection line
additional information
-
Q29587
analysis of lactase phlorizin hydrolase activity in germfree, Lactobacillus fermentum or Escherichia coli monoassociated, Klebsiella pneumoniae LF diassociated and conventional pigs (measuring the amount of D-glucose liberated from lactose). Lowest lactase phlorizin hydrolase activity is observed in pigs, monoassociated with Lactobacillus fermentum. Lactase phlorizin hydrolase activity to mRNA ratio is reduced in enterocytes of pigs monoassociated with Lactobacillus fermentum as compared with germfree pigs
additional information
-
-
10.96 U/g intestinal tissue, membrane bound lactase, after treatment of rats with cortisone; 12.31 U/g intestinal tissue, homogenate, after treatment of rats with cortisone; 1.46 U/g intestinal tissue, soluble lactase, after treatment of rats with cortisone; 2.37 U/g intestinal tissue, soluble lactase; 2.44 U/g intestinal tissue, soluble lactase, after treatment of rats with thyroxine; 4.82 U/g intestinal tissue, membrane bound lactase, after treatment of rats with thyroxine; 7.10 U/g intestinal tissue, homogenate, after treatment of rats with thyroxine; 7.38 U/g intestinal tissue, membrane bound lactase; 9.83 U/g intestinal tissue, homogenate
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.6
6.2
-
-
5.8
6
-
hydrolysis of lactose
6
-
-
activity assay
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4
10
-
pH 4.0: about 60% of maximal activity, pH 10.0: about 50% of maximal activity
5
7
-
pH 5.0: about 70% of maximal activity, pH 7.0: about 65% of maximal activity
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
20
-
-
restoring of enzymatic activity of misfolded mutation G1363S by expression at 20C
25
-
-
activity assay
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
; fully differentiated, intestinal enterocyte-like cells
Manually annotated by BRENDA team
-
predominantly at the apical membrane
Manually annotated by BRENDA team
-
intestinal cells that hydrolyse lactose
Manually annotated by BRENDA team
-
everted sac preparation
Manually annotated by BRENDA team
-
enzyme activity appears to be regulated during development predominantly by transcription mechanism, while alterations during lactation, and along the proximal to distal gradient, are the result of other control mechanism
Manually annotated by BRENDA team
-
brush border membrane of small intestine
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
brush border membrane
Manually annotated by BRENDA team
-
full-length lactase form, mature lactase form with domain III and IV
Manually annotated by BRENDA team
-
water-soluble form of mature lactase with domain III and IV
Manually annotated by BRENDA team
-
misfolded mutation G1363S
Manually annotated by BRENDA team
-
water-soluble form of mature lactase with domain III and IV
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
130000
-
-
molecular mass after N-glycosidase F treatment of LPH; N-glycosylated LPH, gel filtration
160000
-
-
cleaved intestinal mature form of LPH, determinated by pulse-chase experiments; N- and O-glycosylated LPH, gel filtration
220000
-
-
apparent molecular mass of the high-mannose LPH form, determinated by pulse-chase experiments
220000
-
-
determined by SDS-PAGE and Western blot
220000
-
-
Western blot
280000
-
-
hydrophilic enzyme form, gel filtration
320000
-
-
amphiphilic enzyme form, gel filtration
320000
-
-
-
320000
-
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 140000, SDS-PAGE
dimer
-
2 * 160000, SDS-PAGE
dimer
-
2 * 160000, SDS-PAGE
homodimer
-
2* 145000
additional information
-
region LAC236 between R1647 and T1882 is implicated in the dimerization process of pro-enzyme
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
glycoprotein
-
processing of enzyme to a complex glycosylated protein increases in presence of pro-region
glycoprotein
-
existence of two forms of brush border membrane-associated LPH, as N-glycosylated molecule and N- and O-glycosylated molecule; two forms of brush border membrane-associated LPH are known, an N-glycosylated, and an N- and O-glycosylated molecule
proteolytic modification
-
pro-enzyme is cleaved in trans-Golgi network between Arg734 and Leu735, pro-region of enzyme is an intramolecular chaperone, analysis of processing and folding
proteolytic modification
-
human LPH is synthesized as a precursor pro-LPH molecule, pro-LPH undergoes proteolytic cleavage to yield mature LPH by a trypsin-like protease that occurs intracellularly
glycoprotein
-
-
proteolytic modification
-
oral doses of insulin-like growth factor I increase the processing efficiency of pro-enzyme but do not affect enzyme activity
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.5
-
-
30% loss of activity after 0.5 min, 55% loss of activity after 120 min
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
37
-
-
about 30% loss of lactase activity after 30 min
46
-
-
35 min, about 50% loss of phlorizin hydrolase activity. About 70% loss of lactase activity after 25 min
55
-
-
lactase and phlorizin hydrolase activity are identically heat inactivated
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
repeated freezing and thawing, activity declines fast
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-20C, stable for several months
-
4C, stable for several weeks
-
-20C, stable for several months
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expression in HuH-7 cells
-
expression in Cos-1 cells
-
into the pNEB-193 vector
-
expression in HuH-7 cells
-
higher lactase activity and expression of LPH gene in jejunum of rat fed the high-carbohydrate/low-fat (HCT) diet compared with those fed a lowcarbohydrate/high-fat (LCT) diet is regulated at the transcription levels (nuclear run-on assay). In vitro binding of Cdx-2 to CE-LPH1 gene sequence as well as to SIF1 gene sequence is greater in rats fed a HCT diet than those fed a LCT diet
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
D1711N
-
introduction of potential N-glycosilation site, no enzymic activity, probably due to altered protein folding pattern and reduced dimerization efficiency
E1612X
P09848
results in congenital lactase deficiency
G1363S
-
congential lactase deficiency, CLD, mutation, DNA mutation c.4087G->A, genotype compound heterozygote for Y1390X
G1363S
P09848
results in congenital lactase deficiency
G1363S
-
misfolded, rest in endoplasmatic reticulum, congenital lactase deficiency, temperature sensitive
more |
-
CC13910, lactose intolerance, low lactase genotype, no predispose for cataracts with mutation of galactose-1-phosphate uridyl transferase
P1743S
-
introduction of potential N-glycosilation site, no enzymic activity, probably due to altered protein folding pattern and reduced dimerization efficiency
Q268H
-
congential lactase deficiency, CLD, mutation, DNA mutation c.804G->C, genotype compound heterozygote for Y1390X
R1587H
P09848
results in congenital lactase deficiency
S1666fsX1722
-
congential lactase deficiency, CLD, mutation, DNA mutation c.4998_5001delTGAG, genotype compound heterozygote for Y1390X
S218fsX224
-
congential lactase deficiency, CLD, mutation, DNA mutation c.653_654delCT, genotype compound heterozygote for Y1390X
S688P
P09848
results in congenital lactase deficiency
Y1390X
-
congential lactase deficiency, CLD, mutation, DNA mutation c.4170T->A, genotype homozygote
Y1390X
P09848
results in congenital lactase deficiency
I1697N
-
introduction of potential N-glycosilation site, no enzymic activity, probably due to altered protein folding pattern and reduced dimerization efficiency
additional information
-
CT13910, high lactase genotype; TT13910, high lactase genotype
additional information
-
C/C-13910, less enzyme activity, lactase non-persistence, adult type hypolactasia; C/T-13910, less enzyme activity, lactase persistence; T/T-13910, less enzyme activity, lactase persistence
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
medicine
-
findings facilitate genetic testing and enable genetic counseling for congential lactase deficiency, CLD
medicine
-
in irritable bowel syndrome, IBS, patients, genotyping of C/T_13910 and G/A_22018 polymorphisms predicts gastrointestinal symptoms after lactose ingestion and are a diagnostic tool for lactose intolerance
medicine
-
the T/G-13915 variant is the founder mutation of lactase persistence, the result has implications for genetic testing of adult-type hypolactasia
medicine
-
the C allele of the T-13910C polymorphism causing lactose intolerance is associated with lower dietary calcium intake and serum calcium levels but not with bone mineral density or fractures. The associations observed with height and vertebral area were the result of population stratification
medicine
-
among 107 milk-drinking Somali camel-herders from Ethiopia, eight polymorphic sites are identified in the enhancer sequence in an intron of a neighboring gene MCM6 which modulates lactase transcription in vitro. -13915*G and -13907*G are each significantly associated with lactase persistence. Allele -14009*G has borderline association with lactase persistence, but loses significance after correction for multiple testing. Sequence diversity of the enhancer is significantly higher in the lactase persistent members of this and a second cohort compared with non-persistent members of the two groups
medicine
-
analysis of sequence variants of lactase persistence/lactase non-persistence LP/LNP in subjects originating from Northern Russia. The prevalence of the -13910C/C genotype among 148 patients was 28.4%. A G to A variant residing 13914 bp upstream from the LCT gene, -13914G/A, was identified in one participant carrying the -13910C/C genotype. The -13914G/A variant in heterozygous state is associated with increased lactase activity, suggesting that the increased lactase activity is most likely to be associated with the -13914G/A variant
medicine
-
C/T-13910 cis-acting regulatory variant located around 14 kb upstream of lactase gene completely correlates with lactase phenotype in Indian children. The genetic testing for the C/T -13910 variant may be helpful in the diagnosis of adult-type hypolactasia in Indian children
pharmacology
-
BALB/c mouse LPH cDNA provides a necessary foundation for study of the biological function and regulatory mechanism of the lactose intolerance in mice
pharmacology
Mus musculus BALB/c
-
BALB/c mouse LPH cDNA provides a necessary foundation for study of the biological function and regulatory mechanism of the lactose intolerance in mice
-
medicine
-
absorption of quercetin-3-glucoside requires hydrolysis by the enzyme, absorption of quercetin-4-glucoside requires both an interaction with sodium-dependent glucose transporter and hydrolysis by the enzyme
medicine
-
enzyme plays a major role in metabolism of glycosilated phytochemicals
medicine
-
the use of new substrates for the measurement of lactase activity provides a new method for the noninvasive diagnosis of hypolactasia