Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.108 extracted from

  • Day, A.J.; Canada, F.J.; Diaz, J.C.; Kroon, P.A.; Mclauchlan, R.; Faulds, C.B.; Plumb, G.W.; Morgan, M.R.A.; Williamson, G.
    Dietary flavonoids and isoflavone glycosides are hydrolysed by the lactase site of lactase phlorizin hydrolase (2000), FEBS Lett., 468, 166-170.
    View publication on PubMed
Search for more literature for 3.2.1.108

Inhibitors

Inhibitors Comment Organism Structure
2-fluoro-dinitrophenylglucoside specifically inhibits phlorizin hydrolase, lactase activity is inhibited slowly Ovis aries

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 phlorizin pH 6, 37°C Ovis aries
0.044
-
 quercetin-4'-glucoside pH 6, 37°C Ovis aries
0.046
-
 quercetin-3-glucoside pH 6, 37°C Ovis aries
0.085
-
 genistein-7-glucoside pH 6, 37°C Ovis aries
0.149
-
 daidzein-7-glucoside pH 6, 37°C Ovis aries
8.8
-
 lactose pH 6, 37°C Ovis aries

Organism

Organism UniProt Comment Textmining
Ovis aries
-
-
-

Reaction

Reaction Comment Organism Reaction ID
lactose + H2O = D-galactose + D-glucose mechanism Ovis aries
a

Source Tissue

Source Tissue Comment Organism Textmining
small intestine
-
 Ovis aries
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
daidzein 7-O-beta-D-glucopyranoside + H2O
-
 Ovis aries daidzein + beta-D-glucose
-
 ?
genistein-7-glucoside + H2O
-
 Ovis aries genistein + D-glucose
-
 ?
lactose + H2O reaction is not inhibited by 2-fluoro-dinotrophenylglucoside Ovis aries galactose + D-glucose
-
 ?
additional information no substrate: rutin, naringin Ovis aries ?
-
 ?
phlorizin + H2O reaction is inhibited by 2-fluoro-dinotrophenylglucoside Ovis aries ?
-
 ?
quercetin-3-glucoside + H2O reaction is marginally inhibited by 2-fluoro-dinotrophenylglucoside Ovis aries quercetin + D-glucose
-
 ?
quercetin-4'-glucoside + H2O reaction is marginally inhibited by 2-fluoro-dinotrophenylglucoside Ovis aries quercetin + D-glucose
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.5
-
 phlorizin pH 6, 37°C Ovis aries
2.1
-
 daidzein-7-glucoside pH 6, 37°C Ovis aries
6.3
-
 quercetin-3-glucoside pH 6, 37°C Ovis aries
6.5
-
 genistein-7-glucoside pH 6, 37°C Ovis aries
7.5
-
 quercetin-4'-glucoside pH 6, 37°C Ovis aries
33.8
-
 lactose pH 6, 37°C Ovis aries