Information on EC 3.1.3.7 - 3'(2'),5'-bisphosphate nucleotidase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.1.3.7
-
RECOMMENDED NAME
GeneOntology No.
3'(2'),5'-bisphosphate nucleotidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
adenosine 3',5'-bisphosphate + H2O = AMP + phosphate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
-
-
Microbial metabolism in diverse environments
-
-
Sulfur metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
adenosine-3'(2'),5'-bisphosphate 3'(2')-phosphohydrolase
Also acts on 3'-phosphoadenylyl sulfate, and on the corresponding 2'-phosphates.
CAS REGISTRY NUMBER
COMMENTARY hide
9025-83-6
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
formerly known as Arthrospira platensis
UniProt
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
GenBank U33283
Uniprot
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
KP767, KP812, KP656, KP801, KP803
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2'-AMP + H2O
?
show the reaction diagram
-
10% relative activity compared to NADP+
-
-
?
2'-deoxyadenosine 3',5'-bisphosphate + H2O
2'-deoxyadenosine 5'-phosphate + phosphate
show the reaction diagram
2'-deoxycytidine 3',5'-bisphosphate + H2O
2'-deoxycytidine 5'-phosphate + phosphate
show the reaction diagram
2'-deoxyguanosine 3',5'-bisphosphate + H2O
2'-deoxyguanosine 5'-phosphate + phosphate
show the reaction diagram
-
-
-
?
2'-deoxythymidine 3',5'-bisphosphate + H2O
2'-deoxythymidine 5'-phosphate + phosphate
show the reaction diagram
-
-
-
?
2'-deoxyuridine 3',5'-bisphosphate + H2O
2'-deoxyuridine 5'-phosphate + phosphate
show the reaction diagram
-
-
-
?
3'-AMP + H2O
?
show the reaction diagram
-
84% relative activity compared to NADP+
-
-
?
3'-phosphoadenosine 5'-phosphate + H2
AMP + phosphate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenosine 5'-phosphosulfate + H2O
adenosine 5'-phosphosulfate + phosphate
show the reaction diagram
5'-phosphoadenylyl-(3'->5')-adenosine + H2O
? + phosphate
show the reaction diagram
-
-
-
?
adenosine 2',5'-bisphosphate + H2O
adenosine 5'-phosphate + phosphate
show the reaction diagram
adenosine 3',5'-bisphosphate + H2O
adenosine 5'-phosphate + phosphate
show the reaction diagram
adenosine 3',5'-bisphosphate sulfate + H2O
adenosine 5'-phosphate sulfate + phosphate
show the reaction diagram
adenosine 3'-phosphate 5'-phosphosulfate + H2O
adenosine 5'-phosphosulfate + phosphate
show the reaction diagram
CoA + H2O
?
show the reaction diagram
-
-
-
-
?
cytosine 3',5'-bisphosphate + H2O
cytosine 5'-phosphate + phosphate
show the reaction diagram
-
-
-
?
D-fructose 1,6-bisphosphate + H2O
?
show the reaction diagram
D-myo-inositol 2-phosphate + H2O
D-myo-inositol + phosphate
show the reaction diagram
-
9% relative activity compared to NADP+
-
-
?
D-myo-inositol monophosphate + H2O
D-myo-inositol + phosphate
show the reaction diagram
D-myo-inositol-1,4,5-trisphosphate + H2O
?
show the reaction diagram
-
14% relative activity compared to NADP+
-
-
?
D-myo-inositol-1,4-bisphosphate + H2O
?
show the reaction diagram
-
485% relative activity compared to NADP+
-
-
?
guanosine 2',5'-bisphosphate + H2O
guanosine 2'-phosphate + phosphate
show the reaction diagram
-
-
-
?
guanosine 3',5'-bisphosphate + H2O
guanosine 5'-phosphate + phosphate
show the reaction diagram
inositol 1,3,4-trisphosphate + H2O
?
show the reaction diagram
-
-
-
?
inositol 1,3,4-trisphosphate + H2O
inositol 3,4-bisphosphate + phosphate
show the reaction diagram
inositol 1,4-bisphosphate + H2O
inositol 4-phosphate + phosphate
show the reaction diagram
inositol-1,4-bisphosphate + H2O
inositol 4-phosphate + phosphate
show the reaction diagram
N6-methyl-5'-phosphoadenylyl-(3'->5')-adenosine + H2O
? + phosphate
show the reaction diagram
-
-
-
?
NADP+ + H2O
NAD+ + phosphate
show the reaction diagram
-
100% activity
-
-
?
NADPH + H2O
NADH + phosphate
show the reaction diagram
-
145% relative activity compared to NADP+
-
-
?
p-nitrophenyl phosphate
p-nitrophenol + phosphate
show the reaction diagram
-
21% relative activity compared to NADP+
-
-
?
thymidine 3',5'-bisphosphate + H2O
thymidine 5'-phosphate + phosphate
show the reaction diagram
-
-
-
?
uridine 3',5'-bisphosphate + H2O
uridine 5'-phosphate + phosphate
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
adenosine 3',5'-bisphosphate + H2O
adenosine 5'-phosphate + phosphate
show the reaction diagram
O95861
the activity of enzyme is required while 3'-phosphoadenosine 5'-phosphate is a potent inhibitor of a variety of enzymes that use 3'-phosphoadenosine 5'-phosphate as cosubstrate
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
activates slightly
Mn2+
enzyme has a trinuclear metal center in the active site with three Mn2+ ions
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
adenosine 5'-phosphosulfate
-
slight
adenosine-3',5'-bisphosphate
-
inhibition at high concentration
dithiothreitol
-
slight
inositol 1,4-bisphosphate
Mg2+
-
activity is decreased to approximately 70% in the presence of 10 mM MgCl2
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0102
2'-deoxyadenosine 3',5'-bisphosphate
pH 7.5, 3C
-
0.026
2'-deoxycytidine 3',5'-bisphosphate
pH 7.5, 3C
-
0.0052
2'-deoxyguanosine 3',5'-bisphosphate
pH 7.5, 3C
-
0.022
2'-deoxythymidine 3',5'-bisphosphate
pH 7.5, 3C
-
0.029
2'-deoxyuridine 3',5'-bisphosphate
pH 7.5, 3C
-
0.00025
3'-phosphoadenosine 5'-phosphate
-
pH 8.0, temperature not specified in the publication
41.5
5'-ADP
in 50 mM Tris (pH 8.0), 2 mM MgCl2, at 30C
0.0016
5'-phosphoadenylyl-(3'->5')-adenosine
pH 7.5, 3C
-
0.007 - 0.14
adenosine 2',5'-bisphosphate
0.000475 - 1.03
adenosine 3',5'-bisphosphate
0.1 - 0.61
adenosine 3'-phosphate 5'-phosphosulfate
0.014
cytosine 3',5'-bisphosphate
pH 7.5, 3C
-
0.566 - 1.17
D-fructose 1,6-bisphosphate
7.11
D-myo-inositol monophosphate
in 50 mM Tris buffer, pH 8.5, 0.5 mM Mg2+
0.2
Guanosine 2',5'-bisphosphate
-
-
0.0173
guanosine 3',5'-bisphosphate
pH 7.5, 3C
0.25
guanosine-3',5'-bisphosphate
-
-
0.1
inositol 1,3,4-trisphosphate
-
0.077 - 0.113
inositol 1,4-bisphosphate
0.0004
inositol-1,4-bisphosphate
pH 7.5, 30C
0.0054
N6-methyl-5'-phosphoadenylyl-(3'->5')-adenosine
pH 7.5, 3C
-
2.7
NADP+
-
in 10 mM HEPES pH 7.0
2.13
NADPH
-
in 10 mM HEPES pH 7.0
0.0093
thymidine 3',5'-bisphosphate
pH 7.5, 3C
0.0088
uridine 3',5'-bisphosphate
pH 7.5, 3C
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
7.1
2'-deoxyadenosine 3',5'-bisphosphate
Chromobacterium violaceum
Q7NXD4
pH 7.5, 3C
-
9.4
2'-deoxycytidine 3',5'-bisphosphate
Chromobacterium violaceum
Q7NXD4
pH 7.5, 3C
-
4.2
2'-deoxyguanosine 3',5'-bisphosphate
Chromobacterium violaceum
Q7NXD4
pH 7.5, 3C
-
4.3
2'-deoxythymidine 3',5'-bisphosphate
Chromobacterium violaceum
Q7NXD4
pH 7.5, 3C
-
5.2
2'-deoxyuridine 3',5'-bisphosphate
Chromobacterium violaceum
Q7NXD4
pH 7.5, 3C
-
11
3'-phosphoadenosine 5'-phosphate
Saccharomyces cerevisiae
-
pH 8.0, temperature not specified in the publication
0.05
5'-phosphoadenylyl-(3'->5')-adenosine
Chromobacterium violaceum
Q7NXD4
pH 7.5, 3C
-
13.2
adenosine 2',5'-bisphosphate
Escherichia coli
-
in 10 mM HEPES pH 7.0
0.069 - 22
adenosine 3',5'-bisphosphate
8.6
cytosine 3',5'-bisphosphate
Chromobacterium violaceum
Q7NXD4
pH 7.5, 3C
-
0.24 - 1.1
D-fructose 1,6-bisphosphate
0.31
D-myo-inositol monophosphate
Mycobacterium tuberculosis
P9WKJ1
in 50 mM Tris buffer, pH 8.5, 0.5 mM Mg2+
12.3
guanosine 3',5'-bisphosphate
Chromobacterium violaceum
Q7NXD4
pH 7.5, 3C
11.6
N6-methyl-5'-phosphoadenylyl-(3'->5')-adenosine
Chromobacterium violaceum
Q7NXD4
pH 7.5, 3C
-
0.7
NADP+
Escherichia coli
-
in 10 mM HEPES pH 7.0
1.45
NADPH
Escherichia coli
-
in 10 mM HEPES pH 7.0
3.3
thymidine 3',5'-bisphosphate
Chromobacterium violaceum
Q7NXD4
pH 7.5, 3C
5.4
uridine 3',5'-bisphosphate
Chromobacterium violaceum
Q7NXD4
pH 7.5, 3C
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
700
2'-deoxyadenosine 3',5'-bisphosphate
Chromobacterium violaceum
Q7NXD4
pH 7.5, 3C
197727
360
2'-deoxycytidine 3',5'-bisphosphate
Chromobacterium violaceum
Q7NXD4
pH 7.5, 3C
197729
790
2'-deoxyguanosine 3',5'-bisphosphate
Chromobacterium violaceum
Q7NXD4
pH 7.5, 3C
197730
190
2'-deoxythymidine 3',5'-bisphosphate
Chromobacterium violaceum
Q7NXD4
pH 7.5, 3C
197733
180
2'-deoxyuridine 3',5'-bisphosphate
Chromobacterium violaceum
Q7NXD4
pH 7.5, 3C
197732
32
5'-phosphoadenylyl-(3'->5')-adenosine
Chromobacterium violaceum
Q7NXD4
pH 7.5, 3C
197734
1400
adenosine 3',5'-bisphosphate
Chromobacterium violaceum
Q7NXD4
pH 7.5, 3C
68
620
cytosine 3',5'-bisphosphate
Chromobacterium violaceum
Q7NXD4
pH 7.5, 3C
197728
710
guanosine 3',5'-bisphosphate
Chromobacterium violaceum
Q7NXD4
pH 7.5, 3C
93572
2100
N6-methyl-5'-phosphoadenylyl-(3'->5')-adenosine
Chromobacterium violaceum
Q7NXD4
pH 7.5, 3C
197735
360
thymidine 3',5'-bisphosphate
Chromobacterium violaceum
Q7NXD4
pH 7.5, 3C
103683
610
uridine 3',5'-bisphosphate
Chromobacterium violaceum
Q7NXD4
pH 7.5, 3C
197731
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.09 - 0.35
Ca2+
140
K+
pH 8.1, 30C
0.174 - 20
Li+
160
Na+
pH 8.1, 30C
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.035
Ca2+
Saccharomyces cerevisiae
-
pH 8.0, temperature not specified in the publication
2.4 - 3.6
Li+
0.05 - 0.15
LiCl
180 - 600
Na+
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 9
activity range, highest activity in the pH range from pH 7.0 to pH 8.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10 - 75
highest activity at 30-65C, low activity at below 10C, and 28.9% of maximal activity at 75C
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
isoform Bpnt1 is most enriched in the enterocytes of the small intestine and the proximal tubule and thick ascending limb epithelia of the kidneys
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
isoform Bpnt1 is most enriched in the enterocytes of the small intestine and the proximal tubule and thick ascending limb epithelia of the kidneys
Manually annotated by BRENDA team
additional information
-
isoform Bpnt1 is expressed at relatively similar levels in a majority of tissues including the brain, heart, lungs, spleen, pancreas, stomach, liver, small intestine, colon, kidneys, and testes. Bpnt1 is most enriched in the enterocytes of the small intestine and the proximal tubule and thick ascending limb epithelia of the kidneys
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
bound to. Stress markedly influences the accumulation of the membrane-bound enzyme form in the cell. The N-terminal hydrophobic region is necessary for the synthesis of the membrane-bound isoform
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
UNIPROT
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
33000
SDS-PAGE
38000
-
gel filtration
375000
-
calculation from sequence of cDNA
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 38000, SDS-PAGE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
to 1.9 resolution, with AMP and orthophosphate bound in the active site. The enzyme has a trinuclear metal center in the active site with three Mn2+ ions. It belongs to the Cluster of Orthologous Groups cog0613 from the polymerase and histidinol phosphatase family of enzymes
X-ray structure in complex with magnesium and with calcium ions
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
-
the 3'-PAPase activity is decreased to approximately 50% at pH 8.0
677696
10
remained 60% of maximal activity when the pH of the reaction sample is increased to pH 10
677661
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
adenosine 3',5'-bisphosphate-agarose resin column chromatography
Ni-NTA column chromatography
-
nickel affinity chromatography
partial, copurification with other enzymes
-
recombinant enzyme
recombinant protein
-
TALON metal affinity resin column chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cloning of HsPIP and expression in Escherichia coli
expressed in Arabidopsis for genetic complementation
expressed in Escherichia coli
expressed in Escherichia coli BL21 (DE3) cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli K-12 AG1
-
expression in Escherichia coli
expression in Sf9 cells
expression of the Sal1 gene in Escherichia coli
-
GhHL1 gene, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21, overexpression of GhHL1 complements yeast hal2 mutant and enhances yeast growth under elevated NaCl or LiCl
overexpression of the HAL2 gene in Saccharomyces cerevisiae
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
after 1h of dark treatment
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D201N
-
8.2% relative activity compared to the wild type enzyme
D363N
-
6.2% relative activity compared to the wild type enzyme
E133Q
-
4.8% relative activity compared to the wild type enzyme
E301K
-
strongly reduced activity compared to the wild type enzyme
T206A
-
5.6% relative activity compared to the wild type enzyme
V131A
-
strongly reduced activity compared to the wild type enzyme
D141A
less than 5% of the wild-type activity
D141N
less than 5% of the wild-type activity
D144N
less than 5% of the wild-type activity
D292A
less than 5% of the wild-type activity
D292N
less than 5% of the wild-type activity
W291L
less than 5% of the wild-type activity
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
biotechnology
medicine
synthesis
-
construction of a protein expression vector using 3',5'-bisphosphate nucleotidase HAL2 in the presence of Ca2+ as an affinity tag. The tag can be used for procaryotic protein expression and purification by maintaining Ca2+ for efficient affinity binding and chelating Ca2+ for elution
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