Information on EC 3.1.3.7 - 3'(2'),5'-bisphosphate nucleotidase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
3.1.3.7
-
RECOMMENDED NAME
GeneOntology No.
3'(2'),5'-bisphosphate nucleotidase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
adenosine 3',5'-bisphosphate + H2O = AMP + phosphate
show the reaction diagram
-
-
-
-
adenosine 3',5'-bisphosphate + H2O = AMP + phosphate
show the reaction diagram
mechanism
P32179
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis of phosphoric ester
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Metabolic pathways
-
Microbial metabolism in diverse environments
-
Sulfur metabolism
-
SYSTEMATIC NAME
IUBMB Comments
adenosine-3'(2'),5'-bisphosphate 3'(2')-phosphohydrolase
Also acts on 3'-phosphoadenylyl sulfate, and on the corresponding 2'-phosphates.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
3'(2'),5'-bisphosphate nucleotidase
Q38945
-
3'(2'),5'-bisphosphate nucleotidase
Q42546
gene FIERY1, dual enzymatic activity: inositol polyphosphate-1-phosphatase
3'(2'),5'-bisphosphonucleoside 3'(2')-phosphohydrolase
-
-
-
-
3'(2'),5-bisphosphonucleoside 3'(2')-phosphohydrolase
-
-
-
-
3'(2')-phosphoadenosine 5-phosphate phosphatase
O95861
-
3'-PAPase
-
-
3'-phosphoadenosine 5'-phosphate 3'-phosphatase
-
-
3'-phosphoadenosine 5'-phosphate phosphatase
-
-
3'-phosphoadenosine-5'-phosphatase
P65163
-
3'-phosphoadenylylsulfate 3'-phosphatase
-
-
-
-
3(2),5-bisphosphate nucleotidase
Q38945
-
3(2),5-bisphosphate nucleotidase
-
-
3-phosphoadenosine 5-phosphate phosphatase
-
-
3-phosphoadenosine-5-phosphatase
Q3LS17
-
3-phosphoadenosine-5-phosphatase
-
-
3-phosphoesterase
Q94G04
-
adenosine-3',5'-bisphosphate 3'-phosphohydrolase
-
-
-
-
bisphosphate 3'-nucleotidase
-
-
BPNT1
-
-
BPntase
Q38945
-
CysQ
-
exhibits also NADP(H) phosphatase and fructose-1,6-bisphosphatase activities
DPNPase
-
-
-
-
GhHL1
Q8VWZ6
-
GhHL1
Gossypium hirsutum CRI 12
Q8VWZ6
-
-
gPAPP
-
-
Halotolerance protein tol1
-
-
-
-
nucleotidase, phosphoadenylate 3'-
-
-
-
-
PAP 3'-phosphatase
-
-
PAP-phosphatease
O95861
-
PAPase
Q3LS17
-
phosphoadenylate 3'-nucleotidase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9025-83-6
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
-
Q9LKB5
SwissProt
Manually annotated by BRENDA team
GenBank U40433
-
-
Manually annotated by BRENDA team
formerly known as Arthrospira platensis
UniProt
Manually annotated by BRENDA team
Gossypium hirsutum CRI 12
-
UniProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
GenBank AF125042
SwissProt
Manually annotated by BRENDA team
recombinantly expressed in yeast
Uniprot
Manually annotated by BRENDA team
GenBank AF125043
Uniprot
Manually annotated by BRENDA team
GenBank U33283
Uniprot
Manually annotated by BRENDA team
KP767, KP812, KP656, KP801, KP803
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
physiological function
-, Q8VWZ6
overexpression of GhHL1 complements yeast hal2 mutant and enhances yeast growth under elevated NaCl or LiCl, showing a role in salt tolerance associated with ionic stress response
physiological function
Gossypium hirsutum CRI 12
-
overexpression of GhHL1 complements yeast hal2 mutant and enhances yeast growth under elevated NaCl or LiCl, showing a role in salt tolerance associated with ionic stress response
-
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2'-AMP + H2O
?
show the reaction diagram
-
10% relative activity compared to NADP+
-
-
?
3'-AMP + H2O
?
show the reaction diagram
-
84% relative activity compared to NADP+
-
-
?
3'-phosphoadenosine 5'-phosphosulfate + H2O
adenosine 5'-phosphosulfate + phosphate
show the reaction diagram
Q94FY6, Q94G04
-
-
?
3'-phosphoadenosine 5'-phosphosulfate + H2O
adenosine 5'-phosphosulfate + phosphate
show the reaction diagram
Q94FY6, Q94G04
67% enzyme activity in comparison to 3'-phosphoadenosine 5'-phosphate as substrate
-
?
adenosine 2',5'-bisphosphate + H2O
adenosine 5'-phosphate + phosphate
show the reaction diagram
-
-
-
?
adenosine 2',5'-bisphosphate + H2O
adenosine 5'-phosphate + phosphate
show the reaction diagram
-
-
-
-
?
adenosine 2',5'-bisphosphate + H2O
adenosine 5'-phosphate + phosphate
show the reaction diagram
-
-
-
-
?
adenosine 2',5'-bisphosphate + H2O
adenosine 5'-phosphate + phosphate
show the reaction diagram
-
-
-
?
adenosine 2',5'-bisphosphate + H2O
adenosine 5'-phosphate + phosphate
show the reaction diagram
-
-
-
?
adenosine 2',5'-bisphosphate + H2O
adenosine 5'-phosphate + phosphate
show the reaction diagram
P0C5A3
-
-
-
?
adenosine 2',5'-bisphosphate + H2O
adenosine 5'-phosphate + phosphate
show the reaction diagram
O95861
-
-
?
adenosine 2',5'-bisphosphate + H2O
adenosine 5'-phosphate + phosphate
show the reaction diagram
Q38945
-
-
-
?
adenosine 2',5'-bisphosphate + H2O
adenosine 5'-phosphate + phosphate
show the reaction diagram
Q94FY6, Q94G04
100% in comparison to 3'-phosphoadenosine 5'-phosphate as substrate
-
?
adenosine 2',5'-bisphosphate + H2O
adenosine 5'-phosphate + phosphate
show the reaction diagram
Q94FY6, Q94G04
only 6% enzyme activity in comparison to 3'-phosphoadenosine 5'-phosphate as substrate
-
?
adenosine 2',5'-bisphosphate + H2O
adenosine 5'-phosphate + phosphate
show the reaction diagram
-
829% relative activity compared to NADP+
-
-
?
adenosine 3',5'-bisphosphate + H2O
adenosine 5'-phosphate + phosphate
show the reaction diagram
-, Q9Z0S1
-
-
?
adenosine 3',5'-bisphosphate + H2O
adenosine 5'-phosphate + phosphate
show the reaction diagram
Q80V26
-
-
-
?
adenosine 3',5'-bisphosphate + H2O
adenosine 5'-phosphate + phosphate
show the reaction diagram
O95861
-
-
-
?
adenosine 3',5'-bisphosphate + H2O
adenosine 5'-phosphate + phosphate
show the reaction diagram
-
-
-
?
adenosine 3',5'-bisphosphate + H2O
adenosine 5'-phosphate + phosphate
show the reaction diagram
-
-
-
-
?
adenosine 3',5'-bisphosphate + H2O
adenosine 5'-phosphate + phosphate
show the reaction diagram
P32179
-
-
?
adenosine 3',5'-bisphosphate + H2O
adenosine 5'-phosphate + phosphate
show the reaction diagram
-
-
-
?
adenosine 3',5'-bisphosphate + H2O
adenosine 5'-phosphate + phosphate
show the reaction diagram
-
-
-
-
?
adenosine 3',5'-bisphosphate + H2O
adenosine 5'-phosphate + phosphate
show the reaction diagram
-
-
-
?
adenosine 3',5'-bisphosphate + H2O
adenosine 5'-phosphate + phosphate
show the reaction diagram
-
-
-
?
adenosine 3',5'-bisphosphate + H2O
adenosine 5'-phosphate + phosphate
show the reaction diagram
-
-
-
-
?
adenosine 3',5'-bisphosphate + H2O
adenosine 5'-phosphate + phosphate
show the reaction diagram
P0C5A3
-
-
?
adenosine 3',5'-bisphosphate + H2O
adenosine 5'-phosphate + phosphate
show the reaction diagram
-
-
-
-
?
adenosine 3',5'-bisphosphate + H2O
adenosine 5'-phosphate + phosphate
show the reaction diagram
O95861
-
-
?
adenosine 3',5'-bisphosphate + H2O
adenosine 5'-phosphate + phosphate
show the reaction diagram
Q94FY6, Q94G04
-
-
-
-
adenosine 3',5'-bisphosphate + H2O
adenosine 5'-phosphate + phosphate
show the reaction diagram
Q94FY6, Q94G04
-
-
?
adenosine 3',5'-bisphosphate + H2O
adenosine 5'-phosphate + phosphate
show the reaction diagram
Q38945
-
-
-
?
adenosine 3',5'-bisphosphate + H2O
adenosine 5'-phosphate + phosphate
show the reaction diagram
-, Q8VWZ6
-
-
-
?
adenosine 3',5'-bisphosphate + H2O
adenosine 5'-phosphate + phosphate
show the reaction diagram
-, P65163
best substrate
-
-
?
adenosine 3',5'-bisphosphate + H2O
adenosine 5'-phosphate + phosphate
show the reaction diagram
-, Q3LS17
100% activity
-
-
?
adenosine 3',5'-bisphosphate + H2O
adenosine 5'-phosphate + phosphate
show the reaction diagram
O95861
the activity of enzyme is required while 3'-phosphoadenosine 5'-phosphate is a potent inhibitor of a variety of enzymes that use 3'-phosphoadenosine 5'-phosphate as cosubstrate
-
?
adenosine 3',5'-bisphosphate + H2O
adenosine 5'-phosphate + phosphate
show the reaction diagram
-
607% relative activity compared to NADP+
-
-
?
adenosine 3',5'-bisphosphate + H2O
adenosine 5'-phosphate + phosphate
show the reaction diagram
Gossypium hirsutum CRI 12
Q8VWZ6
-
-
-
?
adenosine 3',5'-bisphosphate sulfate + H2O
adenosine 5'-phosphate sulfate + phosphate
show the reaction diagram
-, Q8VWZ6
-
-
-
?
adenosine 3',5'-bisphosphate sulfate + H2O
adenosine 5'-phosphate sulfate + phosphate
show the reaction diagram
Gossypium hirsutum CRI 12
Q8VWZ6
-
-
-
?
adenosine 3'-phosphate 5'-phosphosulfate + H2O
adenosine 5'-phosphosulfate + phosphate
show the reaction diagram
-
-
-
?
adenosine 3'-phosphate 5'-phosphosulfate + H2O
adenosine 5'-phosphosulfate + phosphate
show the reaction diagram
-
-
-
?
adenosine 3'-phosphate 5'-phosphosulfate + H2O
adenosine 5'-phosphosulfate + phosphate
show the reaction diagram
-
-
-
?
adenosine 3'-phosphate 5'-phosphosulfate + H2O
adenosine 5'-phosphosulfate + phosphate
show the reaction diagram
-
-
-
-
?
adenosine 3'-phosphate 5'-phosphosulfate + H2O
adenosine 5'-phosphosulfate + phosphate
show the reaction diagram
-
-
-
?
adenosine 3'-phosphate 5'-phosphosulfate + H2O
adenosine 5'-phosphosulfate + phosphate
show the reaction diagram
P0C5A3
-
-
?
adenosine 3'-phosphate 5'-phosphosulfate + H2O
adenosine 5'-phosphosulfate + phosphate
show the reaction diagram
-, Q3LS17
42% activity compared to adenosine 3',5'bisphosphate
-
-
?
CoA + H2O
?
show the reaction diagram
-
-
-
-
?
D-fructose 1,6-bisphosphate + H2O
?
show the reaction diagram
-, P65163
-
-
-
?
D-fructose 1,6-bisphosphate + H2O
?
show the reaction diagram
-
122% relative activity compared to NADP+
-
-
?
D-myo-inositol 2-phosphate + H2O
D-myo-inositol + phosphate
show the reaction diagram
-
9% relative activity compared to NADP+
-
-
?
D-myo-inositol monophosphate + H2O
D-myo-inositol + phosphate
show the reaction diagram
-, P65163
-
-
-
?
D-myo-inositol monophosphate + H2O
D-myo-inositol + phosphate
show the reaction diagram
-
13% relative activity compared to NADP+
-
-
?
D-myo-inositol-1,4,5-trisphosphate + H2O
?
show the reaction diagram
-
14% relative activity compared to NADP+
-
-
?
D-myo-inositol-1,4-bisphosphate + H2O
?
show the reaction diagram
-
485% relative activity compared to NADP+
-
-
?
guanosine 2',5'-bisphosphate + H2O
guanosine 2'-phosphate + phosphate
show the reaction diagram
-
-
-
?
guanosine 3',5'-bisphosphate + H2O
guanosine 5'-phosphate + phosphate
show the reaction diagram
-
-
-
?
inositol 1,3,4-trisphosphate + H2O
inositol 3,4-bisphosphate + phosphate
show the reaction diagram
-, Q9Z0S1
-
-
?
inositol 1,3,4-trisphosphate + H2O
inositol 3,4-bisphosphate + phosphate
show the reaction diagram
-
-
-
-
?
inositol 1,3,4-trisphosphate + H2O
?
show the reaction diagram
-
-
-
?
inositol 1,4-bisphosphate + H2O
inositol 4-phosphate + phosphate
show the reaction diagram
-, Q9Z0S1
-
-
?
inositol 1,4-bisphosphate + H2O
inositol 4-phosphate + phosphate
show the reaction diagram
-
-
-
-
?
inositol 1,4-bisphosphate + H2O
inositol 4-phosphate + phosphate
show the reaction diagram
-
-
-
?
inositol 1,4-bisphosphate + H2O
inositol 4-phosphate + phosphate
show the reaction diagram
Q94FY6, Q94G04
-
-
?
inositol-1,4-bisphosphate + H2O
inositol 4-phosphate + phosphate
show the reaction diagram
O95861
-
-
?
NADP+ + H2O
NAD+ + phosphate
show the reaction diagram
-
100% activity
-
-
?
NADPH + H2O
NADH + phosphate
show the reaction diagram
-
145% relative activity compared to NADP+
-
-
?
p-nitrophenyl phosphate
p-nitrophenol + phosphate
show the reaction diagram
-
21% relative activity compared to NADP+
-
-
?
inositol-1,4-bisphosphate + H2O
inositol 4-phosphate + phosphate
show the reaction diagram
-, Q3LS17
5.6% activity compared to adenosine 3',5'bisphosphate
-
-
?
additional information
?
-
Q94FY6, Q94G04
not: 3-AMP, ATP, NADP, O-phospho-L-serine, phosphoglycolic acid, L-histidinol phosphate
-
?
additional information
?
-
P0C5A3
adenosine 3',5'-bisphosphate is not the primary substrate, the enzyme may be important for response to salt stress
-
-
-
additional information
?
-
-, Q9NX62
the enzyme mediates lithium toxicity effects
-
-
-
additional information
?
-
-
glycerol phosphate, D-glucose-1-phosphate, D-glucose-6-phosphate, D-fructose-1-phosphate, D-fructose 1,6-bisphosphate, D-fructose-6-phosphate, ADP, 5'-AMP, ATP, NAD+, NMN+, bis-p-nitrophenol phosphate are no substrates
-
-
-
additional information
?
-
Q80V26
3'-phosphoadenosine 5'-phosphate 3'-phosphatase plays a role in the formation of skeletal elements derived through endochondral ossification
-
-
-
additional information
?
-
-, Q8VWZ6
GhHL1 protein efficiently hydrolyzes 3'(2'),5'-bisphosphate nucleotide and 3'(2'),5'-bisphosphate nucleotide sulfate, at 128% of 3'(2'),5'-bisphosphate nucleotide levels, but has low or no activity with other tested compounds including 3'-AMP, 5'-AMP, or ADP. Purified recombinant GhHL1 protein dephosphorylates both 3',5'-bisphosphate nucleotide and inositol 1,4-bisphosphate, demonstrating dual 3',5'-bisphosphate nucleotidase and inositol polyphosphate 1-phosphatase activities, substrate specificity, overview
-
-
-
additional information
?
-
Gossypium hirsutum CRI 12
Q8VWZ6
GhHL1 protein efficiently hydrolyzes 3'(2'),5'-bisphosphate nucleotide and 3'(2'),5'-bisphosphate nucleotide sulfate, at 128% of 3'(2'),5'-bisphosphate nucleotide levels, but has low or no activity with other tested compounds including 3'-AMP, 5'-AMP, or ADP. Purified recombinant GhHL1 protein dephosphorylates both 3',5'-bisphosphate nucleotide and inositol 1,4-bisphosphate, demonstrating dual 3',5'-bisphosphate nucleotidase and inositol polyphosphate 1-phosphatase activities, substrate specificity, overview
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
adenosine 3',5'-bisphosphate + H2O
adenosine 5'-phosphate + phosphate
show the reaction diagram
O95861
the activity of enzyme is required while 3'-phosphoadenosine 5'-phosphate is a potent inhibitor of a variety of enzymes that use 3'-phosphoadenosine 5'-phosphate as cosubstrate
-
?
additional information
?
-
P0C5A3
adenosine 3',5'-bisphosphate is not the primary substrate, the enzyme may be important for response to salt stress
-
-
-
additional information
?
-
-, Q9NX62
the enzyme mediates lithium toxicity effects
-
-
-
additional information
?
-
Q80V26
3'-phosphoadenosine 5'-phosphate 3'-phosphatase plays a role in the formation of skeletal elements derived through endochondral ossification
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ca2+
-
activates slightly
Mg2+
O95861
required, optimal concentration of 1 mM
Mg2+
Q94FY6, Q94G04
required
Mg2+
P32179
required
Mg2+
-, Q3LS17
the activity increases over increasing concentrations of Mg2+ to reach its optimal values in the presence of 2 to 2.5 mM Mg2+
Mg2+
-
in the presence of 10 mM MgCl2, CysQ shows maximum activities of 3'-PAPase and NADP(H)ase at pH 6.4 and pH 8.0, respectively
Mg2+
-, P65163
required for activity, optimal activity observed between pH 8.5 and pH 9.5, using 0.5 mM Mg2+
Mg2+
-, Q8VWZ6
dependent on, optimal at 2 mM
Mg2+
-
activates
Mg2+
P0C5A3
activates; dependend on
Mg2+
-
strict requirement
Mg2+
-
dependend on
additional information
-, Q8VWZ6
GhHL1 is Mg2+-dependent and cation-sensitive
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
adenosine 5'-phosphosulfate
-
slight
adenosine-3',5'-bisphosphate
-
inhibition at high concentration
Ca2+
Q94FY6, Q94G04
;
Ca2+
-
in presence of Mg2+
Ca2+
P0C5A3
-
dithiothreitol
-
slight
inositol 1,4-bisphosphate
O95861
-
inositol 1,4-bisphosphate
-, Q9Z0S1
-
K+
Q94FY6, Q94G04
-
Li+
Q94FY6, Q94G04
;
Li+
P32179
mechanism of lithium inhibition
Li+
Q80V26
inhibited by lithium in vitro
Li+
P0C5A3
-
Li+
-, Q9Z0S1
-
Mg2+
-
activity is decreased to approximately 70% in the presence of 10 mM MgCl2
Na+
Q94FY6, Q94G04
-
Na+
P0C5A3
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
41.5
-
5'-ADP
-, Q3LS17
in 50 mM Tris (pH 8.0), 2 mM MgCl2, at 30C
0.007
-
adenosine 2',5'-bisphosphate
-
-
0.14
-
adenosine 2',5'-bisphosphate
-
in 10 mM HEPES pH 7.0
0.000475
-
adenosine 3',5'-bisphosphate
-, Q9Z0S1
native enzyme
0.00052
-
adenosine 3',5'-bisphosphate
-, Q9Z0S1
recombinant enzyme
0.001
-
adenosine 3',5'-bisphosphate
O95861
pH 7.5, 30C
0.0081
-
adenosine 3',5'-bisphosphate
-, P65163
in 50 mM Tris buffer, pH 8.5, 0.5 mM Mg2+
0.01
-
adenosine 3',5'-bisphosphate
-
-
0.01704
-
adenosine 3',5'-bisphosphate
-
wild type enzyme, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30C
0.02646
-
adenosine 3',5'-bisphosphate
-
mutant enzyme E301K, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30C
0.033
-
adenosine 3',5'-bisphosphate
Q94FY6, Q94G04
pH 8.1, 30C
0.07299
-
adenosine 3',5'-bisphosphate
-
mutant enzyme V131A, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30C
0.126
-
adenosine 3',5'-bisphosphate
Q94FY6, Q94G04
pH 8.1, 30C
0.24
-
adenosine 3',5'-bisphosphate
-
in 10 mM HEPES pH 7.0
0.24
-
adenosine 3',5'-bisphosphate
-
-
1.03
-
adenosine 3',5'-bisphosphate
-, Q3LS17
in 50 mM Tris (pH 8.0), 2 mM MgCl2, at 30C
0.1
-
adenosine 3'-phosphate 5'-phosphosulfate
P0C5A3
-
0.25
-
adenosine 3'-phosphate 5'-phosphosulfate
-
-
0.61
-
adenosine 3'-phosphate 5'-phosphosulfate
-, Q3LS17
in 50 mM Tris (pH 8.0), 2 mM MgCl2, at 30C
0.566
-
D-fructose 1,6-bisphosphate
-, P65163
in 50 mM Tris buffer, pH 8.5, 0.5 mM Mg2+
1.17
-
D-fructose 1,6-bisphosphate
-
in 10 mM HEPES pH 7.0
7.11
-
D-myo-inositol monophosphate
-, P65163
in 50 mM Tris buffer, pH 8.5, 0.5 mM Mg2+
0.2
-
Guanosine 2',5'-bisphosphate
-
-
0.25
-
guanosine-3',5'-bisphosphate
-
-
0.1
-
inositol 1,3,4-trisphosphate
-, Q9Z0S1
-
0.077
-
inositol 1,4-bisphosphate
-
pH 7.8, 30C
0.09
-
inositol 1,4-bisphosphate
-
-
0.113
-
inositol 1,4-bisphosphate
-, Q9Z0S1
-
0.0004
-
inositol-1,4-bisphosphate
O95861
pH 7.5, 30C
2.7
-
NADP+
-
in 10 mM HEPES pH 7.0
2.13
-
NADPH
-
in 10 mM HEPES pH 7.0
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
13.2
-
adenosine 2',5'-bisphosphate
-
in 10 mM HEPES pH 7.0
0.069
-
adenosine 3',5'-bisphosphate
-
mutant enzyme E301K, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30C
1.465
-
adenosine 3',5'-bisphosphate
-
mutant enzyme V131A, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30C
1.921
-
adenosine 3',5'-bisphosphate
-
wild type enzyme, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30C
2.94
-
adenosine 3',5'-bisphosphate
-
wild type enzyme, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30C
5.4
-
adenosine 3',5'-bisphosphate
-, P65163
in 50 mM Tris buffer, pH 8.5, 0.5 mM Mg2+
6.08
-
adenosine 3',5'-bisphosphate
-
mutant enzyme V131A, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30C
11.4
-
adenosine 3',5'-bisphosphate
-
in 10 mM HEPES pH 7.0
0.24
-
D-fructose 1,6-bisphosphate
-, P65163
in 50 mM Tris buffer, pH 8.5, 0.5 mM Mg2+
1.1
-
D-fructose 1,6-bisphosphate
-
in 10 mM HEPES pH 7.0
0.31
-
D-myo-inositol monophosphate
-, P65163
in 50 mM Tris buffer, pH 8.5, 0.5 mM Mg2+
0.7
-
NADP+
-
in 10 mM HEPES pH 7.0
1.45
-
NADPH
-
in 10 mM HEPES pH 7.0
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.09
-
Ca2+
Q94FY6, Q94G04
pH 8.1, 30C
0.35
-
Ca2+
Q94FY6, Q94G04
pH 8.1, 30C
140
-
K+
Q94FY6, Q94G04
pH 8.1, 30C
0.174
-
Li+
Q80V26
in 50 mM Bis-Tris (pH 6.5), 0.05 mM MnCl2, 100 mM KCl
0.3
-
Li+
O95861
pH 7.5, 30C, substrate: adenosine 3,5-bisphosphate
0.6
-
Li+
O95861
pH 7.5, 30C, substrate: inositol-1,4-bisphosphate
1.6
-
Li+
Q94FY6, Q94G04
pH 8.1, 30C
20
-
Li+
Q94FY6, Q94G04
pH 8.1, 30C
160
-
Na+
Q94FY6, Q94G04
pH 8.1, 30C
IC50 VALUE [mM]
IC50 VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2.4
-
Li+
-
wild type enzyme, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30C
3.6
-
Li+
-, Q3LS17
in 50 mM Tris (pH 8.0), 2 mM MgCl2, at 30C
0.05
-
LiCl
-
using adenosine 3',5'-bisphosphate as a substrate
0.15
-
LiCl
-
using adenosine 2',5'-bisphosphate as a substrate
180
-
Na+
-
wild type enzyme, in 50 mM Tris-MES, pH 7.5, 0.5 mM magnesium acetate for 30 min at 30C
600
-
Na+
-, Q3LS17
in 50 mM Tris (pH 8.0), 2 mM MgCl2, at 30C
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.5
-
-, Q8VWZ6
-
8
-
-, Q3LS17
-
8.5
9.5
-, P65163
-
8.5
-
P0C5A3
-
9
-
-
assay at
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.5
9
-, Q8VWZ6
activity range, highest activity in the pH range from pH 7.0 to pH 8.5
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
-
-, Q8VWZ6
-
30
-
-
assay at
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
10
75
-, Q8VWZ6
highest activity at 30-65C, low activity at below 10C, and 28.9% of maximal activity at 75C
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
bound to. Stress markedly influences the accumulation of the membrane-bound enzyme form in the cell. The N-terminal hydrophobic region is necessary for the synthesis of the membrane-bound isoform
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
28000
-
Q94FY6, Q94G04
-
28000
-
-
SDS-PAGE
30000
-
-, P65163
SDS-PAGE
33000
-
O95861
SDS-PAGE
38000
-
-
gel filtration
40000
-
Q94FY6, Q94G04
-
41800
-
-, Q3LS17
SDS-PAGE
44000
-
-
membrane-bound enzyme form
44000
-
P0C5A3
calculation from sequence of cDNA
375000
-
-
calculation from sequence of cDNA
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-, Q8VWZ6
x * 37000, recombinant enzyme, SDS-PAGE
?
P0C5A3
x * 47000, SDS-PAGE
?
Gossypium hirsutum CRI 12
-
x * 37000, recombinant enzyme, SDS-PAGE
-
monomer
-
1 * 38000, SDS-PAGE
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
X-ray structure in complex with magnesium and with calcium ions
P32179
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
8
-
-
the 3'-PAPase activity is decreased to approximately 50% at pH 8.0
10
-
-, Q3LS17
remained 60% of maximal activity when the pH of the reaction sample is increased to pH 10
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
nickel affinity chromatography
-, Q3LS17
partial, copurification with other enzymes
-
Ni-NTA column chromatography
-
TALON metal affinity resin column chromatography
-
-
O95861
recombinant enzyme
O95861
-
-, Q9Z0S1
adenosine 3',5'-bisphosphate-agarose resin column chromatography
-, P65163
recombinant enzyme
-
recombinant enzyme
Q94FY6, Q94G04
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expressed in Arabidopsis for genetic complementation
Q42546
expressed in Escherichia coli
Q38945
expression of the Sal1 gene in Escherichia coli
-
expressed in Escherichia coli
-, Q3LS17
expressed in Escherichia coli BL21 (DE3) cells
-
expressed in Escherichia coli K-12 AG1
-
GhHL1 gene, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21, overexpression of GhHL1 complements yeast hal2 mutant and enhances yeast growth under elevated NaCl or LiCl
-, Q8VWZ6
cloning of HsPIP and expression in Escherichia coli
O95861
expression in Sf9 cells
O95861
expression in Sf9 cells
-, Q9Z0S1
expressed in Escherichia coli BL21(DE3) cells
-, P65163
overexpression of the HAL2 gene in Saccharomyces cerevisiae
-
expressed in Escherichia coli
-
expression in Escherichia coli
Q94FY6, Q94G04
EXPRESSION
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
after 1h of dark treatment
Q42546
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
D201N
-
8.2% relative activity compared to the wild type enzyme
D363N
-
6.2% relative activity compared to the wild type enzyme
E133Q
-
4.8% relative activity compared to the wild type enzyme
E301K
-
strongly reduced activity compared to the wild type enzyme
T206A
-
5.6% relative activity compared to the wild type enzyme
V131A
-
strongly reduced activity compared to the wild type enzyme
D141A
P0C5A3
less than 5% of the wild-type activity
D141N
P0C5A3
less than 5% of the wild-type activity
D144N
P0C5A3
less than 5% of the wild-type activity
D292A
P0C5A3
less than 5% of the wild-type activity
D292N
P0C5A3
less than 5% of the wild-type activity
W291L
P0C5A3
less than 5% of the wild-type activity
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
biotechnology
-, Q8VWZ6
GhHL1 is a functional and good candidate gene that might be used to improve salt tolerance in plants
biotechnology
Gossypium hirsutum CRI 12
-
GhHL1 is a functional and good candidate gene that might be used to improve salt tolerance in plants
-
medicine
O95861
enzyme as a potential target of lithium therapy
medicine
P32179
the enzyme as a potential target of lithium therapy
medicine
-
enzyme as a potential target of lithium therapy