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Literature summary for 3.1.3.7 extracted from

  • Lik-Shing Tsang, M.; Schiff, J.A.
    Properties of enzyme fraction A from Chlorella and copurification of 3 (2 ), 5-biphosphonucleoside 3 (2 )-phosphohydrolase, adenosine 5 phosphosulfate sulfohydrolase and adenosine-5-phosphosulfate cyclase activities (1976), Eur. J. Biochem., 65, 113-121.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
5'-AMP
-
Auxenochlorella pyrenoidosa
adenosine 5'-phosphosulfate slight Auxenochlorella pyrenoidosa
adenosine-3',5'-bisphosphate inhibition at high concentration Auxenochlorella pyrenoidosa
Ca2+ in presence of Mg2+ Auxenochlorella pyrenoidosa
dithiothreitol slight Auxenochlorella pyrenoidosa
Na+ slight Auxenochlorella pyrenoidosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
Guanosine 2',5'-bisphosphate
-
Auxenochlorella pyrenoidosa
0.24
-
adenosine 3',5'-bisphosphate
-
Auxenochlorella pyrenoidosa
0.25
-
adenosine 3'-phosphate 5'-phosphosulfate
-
Auxenochlorella pyrenoidosa
0.25
-
guanosine-3',5'-bisphosphate
-
Auxenochlorella pyrenoidosa

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates slightly Auxenochlorella pyrenoidosa
Mg2+ activates Auxenochlorella pyrenoidosa

Organism

Organism UniProt Comment Textmining
Auxenochlorella pyrenoidosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial, copurification with other enzymes Auxenochlorella pyrenoidosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenosine 2',5'-bisphosphate + H2O
-
Auxenochlorella pyrenoidosa adenosine 5'-phosphate + phosphate
-
?
adenosine 3',5'-bisphosphate + H2O
-
Auxenochlorella pyrenoidosa adenosine 5'-phosphate + phosphate
-
?
adenosine 3'-phosphate 5'-phosphosulfate + H2O
-
Auxenochlorella pyrenoidosa adenosine 5'-phosphosulfate + phosphate
-
?
CoA + H2O
-
Auxenochlorella pyrenoidosa ?
-
?
guanosine 2',5'-bisphosphate + H2O
-
Auxenochlorella pyrenoidosa guanosine 2'-phosphate + phosphate
-
?
guanosine 3',5'-bisphosphate + H2O
-
Auxenochlorella pyrenoidosa guanosine 5'-phosphate + phosphate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Auxenochlorella pyrenoidosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
assay at Auxenochlorella pyrenoidosa