Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.3.7 extracted from

  • Yang, Y.; Ma, J.; Yang, Y.; Zhang, X.; Wang, Y.; Yang, L.; Sun, M.
    Yeast 3,5-bisphosphate nucleotidase: an affinity tag for protein purification (2014), Protein Expr. Purif., 97, 81-87.
    View publication on PubMed

Application

Application Comment Organism
synthesis construction of a protein expression vector using 3',5'-bisphosphate nucleotidase HAL2 in the presence of Ca2+ as an affinity tag. The tag can be used for procaryotic protein expression and purification by maintaining Ca2+ for efficient affinity binding and chelating Ca2+ for elution Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ competitive to Mg2+. Inhibition by Ca2+can be prevented by addition of Mg2+ Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00025
-
3'-phosphoadenosine 5'-phosphate pH 8.0, temperature not specified in the publication Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P32179
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenosine 5'-phosphate + H2
-
Saccharomyces cerevisiae AMP + phosphate
-
?

Synonyms

Synonyms Comment Organism
Hal2
-
Saccharomyces cerevisiae
met22
-
Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
11
-
3'-phosphoadenosine 5'-phosphate pH 8.0, temperature not specified in the publication Saccharomyces cerevisiae

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.035
-
pH 8.0, temperature not specified in the publication Saccharomyces cerevisiae Ca2+