Application | Comment | Organism |
---|---|---|
synthesis | construction of a protein expression vector using 3',5'-bisphosphate nucleotidase HAL2 in the presence of Ca2+ as an affinity tag. The tag can be used for procaryotic protein expression and purification by maintaining Ca2+ for efficient affinity binding and chelating Ca2+ for elution | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | competitive to Mg2+. Inhibition by Ca2+can be prevented by addition of Mg2+ | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00025 | - |
3'-phosphoadenosine 5'-phosphate | pH 8.0, temperature not specified in the publication | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P32179 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenosine 5'-phosphate + H2 | - |
Saccharomyces cerevisiae | AMP + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Hal2 | - |
Saccharomyces cerevisiae |
met22 | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11 | - |
3'-phosphoadenosine 5'-phosphate | pH 8.0, temperature not specified in the publication | Saccharomyces cerevisiae |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.035 | - |
pH 8.0, temperature not specified in the publication | Saccharomyces cerevisiae | Ca2+ |