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Literature summary for 3.1.3.7 extracted from

  • Hatzios, S.K.; Iavarone, A.T.; Bertozzi, C.R.
    Rv2131c from Mycobacterium tuberculosis is a CysQ 3'-phosphoadenosine-5'-phosphatase (2008), Biochemistry, 47, 5823-5831.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.7

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0081
-
 adenosine 3',5'-bisphosphate in 50 mM Tris buffer, pH 8.5, 0.5 mM Mg2+ Mycobacterium tuberculosis
0.566
-
 D-fructose 1,6-bisphosphate in 50 mM Tris buffer, pH 8.5, 0.5 mM Mg2+ Mycobacterium tuberculosis
7.11
-
 D-myo-inositol monophosphate in 50 mM Tris buffer, pH 8.5, 0.5 mM Mg2+ Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity, optimal activity observed between pH 8.5 and pH 9.5, using 0.5 mM Mg2+ Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 SDS-PAGE Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WKJ1
-
-
Mycobacterium tuberculosis H37Rv P9WKJ1
-
-

Purification (Commentary)

Purification (Comment) Organism
adenosine 3',5'-bisphosphate-agarose resin column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenosine 3',5'-bisphosphate + H2O best substrate Mycobacterium tuberculosis adenosine 5'-phosphate + phosphate
-
 ?
adenosine 3',5'-bisphosphate + H2O best substrate Mycobacterium tuberculosis H37Rv adenosine 5'-phosphate + phosphate
-
 ?
D-fructose 1,6-bisphosphate + H2O
-
 Mycobacterium tuberculosis ?
-
 ?
D-fructose 1,6-bisphosphate + H2O
-
 Mycobacterium tuberculosis H37Rv ?
-
 ?
D-myo-inositol monophosphate + H2O
-
 Mycobacterium tuberculosis D-myo-inositol + phosphate
-
 ?
D-myo-inositol monophosphate + H2O
-
 Mycobacterium tuberculosis H37Rv D-myo-inositol + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
3'-phosphoadenosine-5'-phosphatase
-
 Mycobacterium tuberculosis
CysQ
-
 Mycobacterium tuberculosis
Rv2131c
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.24
-
 D-fructose 1,6-bisphosphate in 50 mM Tris buffer, pH 8.5, 0.5 mM Mg2+ Mycobacterium tuberculosis
0.31
-
 D-myo-inositol monophosphate in 50 mM Tris buffer, pH 8.5, 0.5 mM Mg2+ Mycobacterium tuberculosis
5.4
-
 adenosine 3',5'-bisphosphate in 50 mM Tris buffer, pH 8.5, 0.5 mM Mg2+ Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9.5
-
 Mycobacterium tuberculosis