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Literature summary for 3.1.3.7 extracted from
- NADP(H) phosphatase activities of archaeal inositol monophosphatase and eubacterial 3-phosphoadenosine 5-phosphate phosphatase (2007), Appl. Environ. Microbiol., 73, 5447-5452.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli K-12 AG1 | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| LiCl | - |
Escherichia coli |  |
| Mg2+ | activity is decreased to approximately 70% in the presence of 10 mM MgCl2 | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.14 | - |
adenosine 2',5'-bisphosphate | in 10 mM HEPES pH 7.0 | Escherichia coli | |
| 0.24 | - |
adenosine 3',5'-bisphosphate | in 10 mM HEPES pH 7.0 | Escherichia coli | |
| 1.17 | - |
D-fructose 1,6-bisphosphate | in 10 mM HEPES pH 7.0 | Escherichia coli | |
| 2.13 | - |
NADPH | in 10 mM HEPES pH 7.0 | Escherichia coli | |
| 2.7 | - |
NADP+ | in 10 mM HEPES pH 7.0 | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | in the presence of 10 mM MgCl2, CysQ shows maximum activities of 3'-PAPase and NADP(H)ase at pH 6.4 and pH 8.0, respectively | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
SDS-PAGE | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| TALON metal affinity resin column chromatography | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-AMP + H2O | 10% relative activity compared to NADP+ | Escherichia coli | ? | - |
? | |
| 3'-AMP + H2O | 84% relative activity compared to NADP+ | Escherichia coli | ? | - |
? | |
| adenosine 2',5'-bisphosphate + H2O | 829% relative activity compared to NADP+ | Escherichia coli | adenosine 5'-phosphate + phosphate | - |
? | |
| adenosine 3',5'-bisphosphate + H2O | 607% relative activity compared to NADP+ | Escherichia coli | adenosine 5'-phosphate + phosphate | - |
? | |
| D-fructose 1,6-bisphosphate + H2O | 122% relative activity compared to NADP+ | Escherichia coli | ? | - |
? | |
| D-myo-inositol 2-phosphate + H2O | 9% relative activity compared to NADP+ | Escherichia coli | D-myo-inositol + phosphate | - |
? | |
| D-myo-inositol monophosphate + H2O | 13% relative activity compared to NADP+ | Escherichia coli | D-myo-inositol + phosphate | - |
? | |
| D-myo-inositol-1,4,5-trisphosphate + H2O | 14% relative activity compared to NADP+ | Escherichia coli | ? | - |
? | |
| D-myo-inositol-1,4-bisphosphate + H2O | 485% relative activity compared to NADP+ | Escherichia coli | ? | - |
? | |
| additional information | glycerol phosphate, D-glucose-1-phosphate, D-glucose-6-phosphate, D-fructose-1-phosphate, D-fructose 1,6-bisphosphate, D-fructose-6-phosphate, ADP, 5'-AMP, ATP, NAD+, NMN+, bis-p-nitrophenol phosphate are no substrates | Escherichia coli | ? | - |
? | |
| NADP+ + H2O | 100% activity | Escherichia coli | NAD+ + phosphate | - |
? | |
| NADPH + H2O | 145% relative activity compared to NADP+ | Escherichia coli | NADH + phosphate | - |
? | |
| p-nitrophenyl phosphate | 21% relative activity compared to NADP+ | Escherichia coli | p-nitrophenol + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3'-PAPase | - |
Escherichia coli |
| 3'-phosphoadenosine 5'-phosphate phosphatase | - |
Escherichia coli |
| 3-phosphoadenosine 5-phosphate phosphatase | - |
Escherichia coli |
| CysQ | exhibits also NADP(H) phosphatase and fructose-1,6-bisphosphatase activities | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.7 | - |
NADP+ | in 10 mM HEPES pH 7.0 | Escherichia coli | |
| 1.1 | - |
D-fructose 1,6-bisphosphate | in 10 mM HEPES pH 7.0 | Escherichia coli | |
| 1.45 | - |
NADPH | in 10 mM HEPES pH 7.0 | Escherichia coli | |
| 11.4 | - |
adenosine 3',5'-bisphosphate | in 10 mM HEPES pH 7.0 | Escherichia coli | |
| 13.2 | - |
adenosine 2',5'-bisphosphate | in 10 mM HEPES pH 7.0 | Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.4 | - |
- |
Escherichia coli |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
the 3'-PAPase activity is decreased to approximately 50% at pH 8.0 | Escherichia coli |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
using adenosine 3',5'-bisphosphate as a substrate | Escherichia coli | LiCl | |
| 0.15 | - |
using adenosine 2',5'-bisphosphate as a substrate | Escherichia coli | LiCl | |
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