Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Arthrospira platensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Li+ | - |
Arthrospira platensis | |
Na+ | - |
Arthrospira platensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.61 | - |
adenosine 3'-phosphate 5'-phosphosulfate | in 50 mM Tris (pH 8.0), 2 mM MgCl2, at 30°C | Arthrospira platensis | |
1.03 | - |
adenosine 3',5'-bisphosphate | in 50 mM Tris (pH 8.0), 2 mM MgCl2, at 30°C | Arthrospira platensis | |
41.5 | - |
5'-ADP | in 50 mM Tris (pH 8.0), 2 mM MgCl2, at 30°C | Arthrospira platensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | the activity increases over increasing concentrations of Mg2+ to reach its optimal values in the presence of 2 to 2.5 mM Mg2+ | Arthrospira platensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41800 | - |
SDS-PAGE | Arthrospira platensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrospira platensis | Q3LS17 | formerly known as Arthrospira platensis | - |
Purification (Comment) | Organism |
---|---|
nickel affinity chromatography | Arthrospira platensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-ADP + H2O | - |
Arthrospira platensis | ? | - |
? | |
adenosine 3',5'-bisphosphate + H2O | 100% activity | Arthrospira platensis | adenosine 5'-phosphate + phosphate | - |
? | |
adenosine 3'-phosphate 5'-phosphosulfate + H2O | 42% activity compared to adenosine 3',5'bisphosphate | Arthrospira platensis | adenosine 5'-phosphosulfate + phosphate | - |
? | |
inositol-1,4-bisphosphate + H2O | 5.6% activity compared to adenosine 3',5'bisphosphate | Arthrospira platensis | inositol 4-phosphate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-phosphoadenosine-5-phosphatase | - |
Arthrospira platensis |
HalA | - |
Arthrospira platensis |
PAPase | - |
Arthrospira platensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Arthrospira platensis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
10 | - |
remained 60% of maximal activity when the pH of the reaction sample is increased to pH 10 | Arthrospira platensis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
3.6 | - |
in 50 mM Tris (pH 8.0), 2 mM MgCl2, at 30°C | Arthrospira platensis | Li+ | |
600 | - |
in 50 mM Tris (pH 8.0), 2 mM MgCl2, at 30°C | Arthrospira platensis | Na+ |