EC Number   |
Protein Variants |
Reference |
|---|
    6.2.1.12 | A251S |
the mutant enzyme shows a lower binding affinity and catalytic efficiency than the wild type enzyme |
746000 |
| 6.2.1.12 | A251S/M247Y |
the mutant enzyme shows a lower binding affinity and catalytic efficiency than the wild type enzyme |
746000 |
| 6.2.1.12 | C403A |
activity reduced to 45% of wild-type level, no effect on Km values for ATP and caffeate |
651262 |
| 6.2.1.12 | E340A |
4CL1-mutant, activity almost completely abolished |
652422 |
| 6.2.1.12 | E401Q |
activity reduced to 21% of wild-type level, no effect on Km values for ATP and caffeate |
651262 |
| 6.2.1.12 | F239A |
site-directed mutagenesis, the mutant strain produces more naringenin chalcone compared to the wild-type |
728481 |
| 6.2.1.12 | F239N |
site-directed mutagenesis, the mutant strain produces more naringenin chalcone compared to the wild-type |
728481 |
| 6.2.1.12 | F239R |
site-directed mutagenesis, the mutant strain produces more naringenin chalcone compared to the wild-type |
728481 |
| 6.2.1.12 | F239S |
site-directed mutagenesis, the mutant shows increased activity in vivo, and in vitro with coumaric acid, but reduced activity with ferulic acid compared to the wild-type enzyme. The mutant strain produces more naringenin chalcone compared to the wild-type |
728481 |
| 6.2.1.12 | F269G |
site-directed mutagenesis, the mutant strain produces less naringenin chalcone compared to the wild-type |
728481 |
