Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Plagiochasma appendiculatum |
Protein Variants | Comment | Organism |
---|---|---|
A251S | the mutant enzyme shows a lower binding affinity and catalytic efficiency than the wild type enzyme | Plagiochasma appendiculatum |
A251S/M247Y | the mutant enzyme shows a lower binding affinity and catalytic efficiency than the wild type enzyme | Plagiochasma appendiculatum |
M247Y | the catalytic efficiency of, 4-coumaric, caffeic and dihydro-4-coumaric acids are higher in the mutant than in the wild type enzyme | Plagiochasma appendiculatum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.155 | - |
4-coumarate | mutant enzyme M247Y, at pH 7.5 and 30°C | Plagiochasma appendiculatum | |
0.1887 | - |
4-coumarate | wild type enzyme, at pH 7.5 and 30°C | Plagiochasma appendiculatum | |
0.1929 | - |
caffeate | mutant enzyme M247Y, at pH 7.5 and 30°C | Plagiochasma appendiculatum | |
0.2089 | - |
caffeate | wild type enzyme, at pH 7.5 and 30°C | Plagiochasma appendiculatum | |
0.253 | - |
dihydro-4-coumarate | mutant enzyme M247Y, at pH 7.5 and 30°C | Plagiochasma appendiculatum | |
0.283 | - |
4-coumarate | mutant enzyme M247Y/A251S, at pH 7.5 and 30°C | Plagiochasma appendiculatum | |
0.389 | - |
4-coumarate | mutant enzyme A251S, at pH 7.5 and 30°C | Plagiochasma appendiculatum | |
0.4418 | - |
dihydro-4-coumarate | wild type enzyme, at pH 7.5 and 30°C | Plagiochasma appendiculatum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-coumarate + CoA | Plagiochasma appendiculatum | 4-coumarate is the most favorable substrate | AMP + diphosphate + 4-coumaroyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plagiochasma appendiculatum | A0A0D4C3L0 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.008 | 4 | mutant enzyme M247Y/A251S, with dihydro-4-coumarate as substrate, at pH 7.5 and 30°C | Plagiochasma appendiculatum |
0.0093 | - |
mutant enzyme A251S, with dihydro-4-coumarate as substrate, at pH 7.5 and 30°C | Plagiochasma appendiculatum |
0.0135 | - |
mutant enzyme A251S, with caffeate as substrate, at pH 7.5 and 30°C | Plagiochasma appendiculatum |
0.0152 | - |
mutant enzyme M247Y/A251S, with cinnamate as substrate, at pH 7.5 and 30°C | Plagiochasma appendiculatum |
0.0161 | - |
mutant enzyme M247Y/A251S, with caffeate as substrate, at pH 7.5 and 30°C | Plagiochasma appendiculatum |
0.0204 | - |
wild type enzyme, with dihydro-4-coumarate as substrate, at pH 7.5 and 30°C | Plagiochasma appendiculatum |
0.0231 | - |
mutant enzyme A251S, with cinnamate as substrate, at pH 7.5 and 30°C | Plagiochasma appendiculatum |
0.0344 | - |
mutant enzyme M247Y, with dihydro-4-coumarate as substrate, at pH 7.5 and 30°C | Plagiochasma appendiculatum |
0.0378 | - |
wild type enzyme, with cinnamate as substrate, at pH 7.5 and 30°C | Plagiochasma appendiculatum |
0.0409 | - |
wild type enzyme, with caffeate as substrate, at pH 7.5 and 30°C | Plagiochasma appendiculatum |
0.0577 | - |
mutant enzyme M247Y/A251S, with 4-coumarate as substrate, at pH 7.5 and 30°C | Plagiochasma appendiculatum |
0.0706 | - |
mutant enzyme A251S, with 4-coumarate as substrate, at pH 7.5 and 30°C | Plagiochasma appendiculatum |
0.1079 | - |
mutant enzyme M247Y, with cinnamate as substrate, at pH 7.5 and 30°C | Plagiochasma appendiculatum |
0.1136 | - |
wild type enzyme, with 4-coumarate as substrate, at pH 7.5 and 30°C | Plagiochasma appendiculatum |
0.1309 | - |
mutant enzyme M247Y, with caffeate as substrate, at pH 7.5 and 30°C | Plagiochasma appendiculatum |
0.4561 | - |
mutant enzyme M247Y, with 4-coumarate as substrate, at pH 7.5 and 30°C | Plagiochasma appendiculatum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-coumarate + CoA | - |
Plagiochasma appendiculatum | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
ATP + 4-coumarate + CoA | 4-coumarate is the most favorable substrate | Plagiochasma appendiculatum | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
ATP + caffeate + CoA | - |
Plagiochasma appendiculatum | AMP + diphosphate + caffeoyl-CoA | - |
? | |
ATP + cinnamate + CoA | - |
Plagiochasma appendiculatum | AMP + diphosphate + cinnamoyl-CoA | - |
? | |
ATP + dihydro-4-coumarate + CoA | - |
Plagiochasma appendiculatum | AMP + diphosphate + dihydro-4-coumaroyl-CoA | - |
? | |
additional information | no activity with ferulate, 5-hydroxyferylate, and sinapate | Plagiochasma appendiculatum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50920, calculated from amino acid sequence | Plagiochasma appendiculatum |
? | x * 80000, thioredoxin-, His- and S-tagged enzyme, SDS-PAGE | Plagiochasma appendiculatum |
Synonyms | Comment | Organism |
---|---|---|
4-coumarate CoA ligase | - |
Plagiochasma appendiculatum |
4-coumarate: coenzyme A ligase | - |
Plagiochasma appendiculatum |
4CL1 | - |
Plagiochasma appendiculatum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.084 | - |
caffeate | wild type enzyme, at pH 7.5 and 30°C | Plagiochasma appendiculatum | |
0.084 | - |
dihydro-4-coumarate | wild type enzyme, at pH 7.5 and 30°C | Plagiochasma appendiculatum | |
0.098 | - |
dihydro-4-coumarate | mutant enzyme M247Y, at pH 7.5 and 30°C | Plagiochasma appendiculatum | |
0.123 | - |
4-coumarate | mutant enzyme M247Y/A251S, at pH 7.5 and 30°C | Plagiochasma appendiculatum | |
0.216 | - |
4-coumarate | wild type enzyme, at pH 7.5 and 30°C | Plagiochasma appendiculatum | |
0.217 | - |
caffeate | mutant enzyme M247Y, at pH 7.5 and 30°C | Plagiochasma appendiculatum | |
0.221 | - |
4-coumarate | mutant enzyme A251S, at pH 7.5 and 30°C | Plagiochasma appendiculatum | |
0.782 | - |
4-coumarate | mutant enzyme M247Y, at pH 7.5 and 30°C | Plagiochasma appendiculatum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Plagiochasma appendiculatum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Plagiochasma appendiculatum | calculated from amino acid sequence | - |
9.7 |
Organism | Comment | Expression |
---|---|---|
Plagiochasma appendiculatum | enzyme gene transcription is induced when Plagiochasma appendiculatum thallus is treated with either salicylic acid or methyl jasmonate | up |