Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli M15[pRP4] as His-tag fusion protein | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
C403A | activity reduced to 45% of wild-type level, no effect on Km values for ATP and caffeate | Arabidopsis thaliana |
E401Q | activity reduced to 21% of wild-type level, no effect on Km values for ATP and caffeate | Arabidopsis thaliana |
K211S | activity reduced to 2.7% of wild-type level | Arabidopsis thaliana |
K445T | activity reduced to 0.2% of wild-type level | Arabidopsis thaliana |
K457S | activity reduced to 4% of wild-type level | Arabidopsis thaliana |
K540N | no activity | Arabidopsis thaliana |
R449Q | activity reduced to 3% of wild-type level, Km values significantly increased | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
caffeate | K457S mutant enzyme | Arabidopsis thaliana | |
0.014 | - |
caffeate | K211S mutant enzyme | Arabidopsis thaliana | |
0.018 | - |
caffeate | C403A mutant enzyme | Arabidopsis thaliana | |
0.024 | - |
caffeate | wild type | Arabidopsis thaliana | |
0.028 | - |
caffeate | E401Q mutant enzyme | Arabidopsis thaliana | |
0.029 | - |
ATP | K457S mutant enzyme | Arabidopsis thaliana | |
0.151 | - |
ATP | K211S mutant enzyme | Arabidopsis thaliana | |
0.163 | - |
ATP | wild type | Arabidopsis thaliana | |
0.173 | - |
ATP | C403 mutant enzyme | Arabidopsis thaliana | |
0.24 | - |
ATP | E401Q mutant enzyme | Arabidopsis thaliana | |
0.4 | - |
caffeate | R449Q mutant enzyme | Arabidopsis thaliana | |
1.507 | - |
ATP | R449Q mutant enzyme | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-coumarate + CoA | Arabidopsis thaliana | plant phenylpropanoid metabolism | AMP + diphosphate + 4-coumaroyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
isoform 4CL2 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme using His-tag | Arabidopsis thaliana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.34 | - |
K211S mutant enzyme with caffeate as substrate | Arabidopsis thaliana |
0.37 | - |
R449Q mutant enzyme with caffeate as substrate | Arabidopsis thaliana |
0.46 | - |
K457S mutant enzyme with caffeate as substrate | Arabidopsis thaliana |
2.64 | - |
E401Q mutant enzyme with caffeate as substrate | Arabidopsis thaliana |
5.64 | - |
C403A mutant enzyme with caffeate as substrate | Arabidopsis thaliana |
12.5 | - |
wild type with caffeate as substrate | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-coumarate + CoA | - |
Arabidopsis thaliana | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
ATP + 4-coumarate + CoA | plant phenylpropanoid metabolism | Arabidopsis thaliana | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
ATP + caffeate + CoA | - |
Arabidopsis thaliana | AMP + diphosphate + caffeoyl-CoA | - |
? |