EC Number   |
Protein Variants |
Reference |
|---|
    4.1.1.33 | C210S |
decrease in melting temperature by 12 degrees |
-, 734098 |
| 4.1.1.33 | D281N |
site-directed mutagenesis, the mutant only shows reduced diphosphomevalonate 3-kinase activity and phosphate elimination/decarboxylation |
-, 759486 |
| 4.1.1.33 | D281T |
site-directed mutagenesis, the mutant only shows reduced diphosphomevalonate 3-kinase activity compared to wild-type, but is inactive in the process of phosphate elimination/decarboxylation |
-, 759486 |
| 4.1.1.33 | D281V |
site-directed mutagenesis, the mutant only shows reduced diphosphomevalonate 3-kinase activity compared to wild-type, but is inactive in the process of phosphate elimination/decarboxylation |
-, 759486 |
| 4.1.1.33 | D283A |
10000fold decrease in kcat value |
726990 |
| 4.1.1.33 | D283A |
catalytically deficient enzyme with 100000fold decreased kcat value |
726990 |
| 4.1.1.33 | D302A |
1000fold decrease in kcat value, still retains ability to stoichiometrically bind nucleotide triphosphates at the active site |
666848 |
| 4.1.1.33 | D302N |
100000fold decrease in kcat value, still retains ability to stoichiometrically bind nucleotide triphosphates at the active site |
666848 |
| 4.1.1.33 | D306A |
low activity |
678692 |
| 4.1.1.33 | D306E |
low activity |
678692 |
