EC Number   |
Protein Variants |
Reference |
|---|
   2.4.1.10 | A154S |
the mutant shows reduced activity compared to the wild type enzyme |
756945 |
| 2.4.1.10 | A344P |
site directed mutagenesis, same behavior like the wild-type |
689854 |
| 2.4.1.10 | C339S |
kcat/Km for hydrolysis of sucrose is 68.75fold lower than wild-type value |
-, 671836 |
| 2.4.1.10 | C395S |
kcat/Km for hydrolysis of sucrose is 60fold lower than wild-type value |
-, 671836 |
| 2.4.1.10 | D135N |
kcat/Km for hydrolysis of sucrose is 1257fold lower than wild-type value |
-, 671836 |
| 2.4.1.10 | D194N |
site-directed mutagenesis, 3400fold decreased kcat and 3fold increased Km for sucrose, activity with sucrose similar to wild-type, reduced activity with raffinose and stachyose |
488343 |
| 2.4.1.10 | D194N |
the mutant shows 8300fold decrease in catalytic efficiency compared to the wild type enzyme |
756347 |
| 2.4.1.10 | D219A |
inactive |
756347 |
| 2.4.1.10 | D219A |
site-directed mutagenesis, inactive mutant |
-, 736849, 736891 |
| 2.4.1.10 | D225A |
inactive |
756945 |
