Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutants in Escherichia coli | Zymomonas mobilis |
Protein Variants | Comment | Organism |
---|---|---|
D194N | site-directed mutagenesis, 3400fold decreased kcat and 3fold increased Km for sucrose, activity with sucrose similar to wild-type, reduced activity with raffinose and stachyose | Zymomonas mobilis |
D275N | site-directed mutagenesis, enhanced kcat and Km for sucrose, activity with sucrose similar to wild-type, reduced activity with raffinose and stachyose | Zymomonas mobilis |
D302N | site-directed mutagenesis, enhanced kcat and Km for sucrose, activity with sucrose similar to wild-type, reduced activity with raffinose and stachyose | Zymomonas mobilis |
D308N | site-directed mutagenesis, enhanced kcat and Km for sucrose, activity with sucrose similar to wild-type, reduced activity with raffinose and stachyose | Zymomonas mobilis |
E117Q | site-directed mutagenesis, slightly reduced kcat and enhanced Km for sucrose, slightly enhanced activity with sucrose, reduced activity with raffinose and stachyose | Zymomonas mobilis |
E211Q | site-directed mutagenesis, highly reduced kcat and enhanced Km for sucrose, highly reduced activity with sucrose, reduced activity with raffinose, increased activity with stachyose | Zymomonas mobilis |
E278D | random mutagenesis, 30fold reduced kcat and reduced Km for sucrose, slightly enhanced activity with sucrose, reduced activity with raffinose | Zymomonas mobilis |
E278H | site-directed mutagenesis, 210fold reduced kcat and enhanced Km for sucrose, reduced activity with sucrose and reduced activity with raffinose | Zymomonas mobilis |
H296K | site-directed mutagenesis, reduced kcat and highly increased Km for sucrose, unaltered activity with sucrose, increased activity with raffinose, highly increased activity with stachyose | Zymomonas mobilis |
H296L | site-directed mutagenesis, reduced kcat and highly increased Km for sucrose, unaltered activity with sucrose, increased activity with raffinose, highly increased activity with stachyose | Zymomonas mobilis |
H296R | site-directed mutagenesis, reduced kcat and highly increased Km for sucrose, unaltered activity with sucrose, increased activity with raffinose, highly increased activity with stachyose | Zymomonas mobilis |
H296S | site-directed mutagenesis, reduced kcat and highly increased Km for sucrose, unaltered activity with sucrose, increased activity with raffinose, highly increased activity with stachyose | Zymomonas mobilis |
Q339H/P340A | random mutagenesis, reduced activity with stachyose, slightly enhanced kcat and enhanced Km for sucrose, slightly reduced activity with sucrose | Zymomonas mobilis |
V223A | random mutagenesis, reduced kcat, highly enhanced Km, and reduced activity with sucrose, enhanced activity with raffinose and 3.8fold with stachyose | Zymomonas mobilis |
W80R | random mutagenesis, reduced kcat for sucrose, enhanced Km, reduced activity with sucrose, slightly reduced activity with raffinose | Zymomonas mobilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | mutants, overview | Zymomonas mobilis | |
125 | - |
sucrose | wild-type enzyme | Zymomonas mobilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
alignment of conserved motifs in amino acid sequence | Zymomonas mobilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zymomonas mobilis | - |
gene sucZE2, strain IFO13756 | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside = D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside | Glu278, located in DXXER conserved motif, and Asp194, located in conserved RDP motif, and His296 are involved in catalysis | Zymomonas mobilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
raffinose + (2,6-beta-D-fructosyl)n | - |
Zymomonas mobilis | galactose + (2,6-beta-D-fructosyl)n+1 | - |
? | |
stachyose + (2,6-beta-D-fructosyl)n | - |
Zymomonas mobilis | ? | - |
? | |
sucrose + (2,6-beta-D-fructosyl)n | - |
Zymomonas mobilis | glucose + (2,6-beta-D-fructosyl)n+1 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | mutants, overview | Zymomonas mobilis | |
28.3 | - |
sucrose | wild-type enzyme | Zymomonas mobilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
assay at | Zymomonas mobilis |