Information on EC 2.4.1.10 - levansucrase

Substrates: mutant enzymes H243L and S164A synthesize either high or low levan molecular weight
Products: -

sucrose + maltose

sucrose + maltose

D-glucose + alpha-D-glucopyranosyl-(1->4)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside

sucrose + maltose

erlose + D-glucose

Substrates: -
Products: -

sucrose + maltotriose

Substrates: -
Products: -

sucrose + mannose

Substrates: -
Products: -

sucrose + melibiose

Substrates: -
Products: -

sucrose + melibiose

D-glucose + raffinose

sucrose + melibiose

raffinose + D-glucose

sucrose + methanol

glucose + methyl-beta-D-fructoside

sucrose + polygalacturonic acid

Substrates: -
Products: -

sucrose + raffinose

Fru(beta-2,1)Gal + fructosylated lactose + fructosylated cellobiose + 1-kestotriose + 6-kestotriose + ?

Substrates: -
Products: -

sucrose + raffinose

melibiose + ?

Substrates: -
Products: -

sucrose + raffinose

melibiose + D-fructose + levan

Substrates: -
Products: -

sucrose + sorbitol

sucrose + stachyose

manninotriose + D-fructose

Substrates: -
Products: -

sucrose + sucrose

1-kestose + 6-kestose + neo-kestose + ?

6 entries

sucrose + sucrose

6-kestose + levanbiose + blastose + 1-kestose + ?

sucrose + sucrose

sucrose + sucrose

D-glucose + (2,6-beta-D-fructosyl)3

Q60114

Substrates: dimer form
Products: degree of polymerisation 3

sucrose + sucrose

D-glucose + 1-kestose

sucrose + sucrose

D-glucose + 1-kestose + nystose + ?

sucrose + sucrose

D-glucose + 6-kestose

sucrose + sucrose

D-glucose + 6-kestose + 6-nystose + 6,6,6-kestopentaose + levanbiose + levantriose + ?

sucrose + sucrose

D-glucose + levan

22 entries

sucrose + sucrose

D-glucose + levan + fructooligosaccharides

Substrates: -
Products: -

sucrose + sucrose

D-glucose + levan type-fructan

sucrose + sucrose

D-glucose + low-molecular weight levan + short-chain fructooligosaccharides (DP 3-6)

sucrose + sucrose

D-glucose + neokestose

Substrates: -
Products: identified by one-dimensional and correlation spectroscopy (i.e. COSY, TOCSY, HMBC, DEPT and HSQC)

sucrose + sucrose

D-glucose + nystose

Substrates: -
Products: identified by HPAEC

sucrose + sucrose

kestose + D-glucose

Substrates: high concentration of sucrose
Products: -

sucrose + triisopropylbenzenesulfonyllevan

488320

Substrates: -
Products: -

sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside

D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside

Q60114

Substrates: pH lower than 7.0, fibril form
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

41 entries

surgarcane molasse

D-glucose + fructose + kestose + nystose + difructose + levan

Substrates: -
Products: -

sweet sorghum juice

D-glucose + fructose + kestose + nystose + difructose + levan

Substrates: -
Products: -

table sugar

D-glucose + fructose + kestose + nystose + difructose + levan

Substrates: -
Products: -

additional information

82 entries

2 sucrose

6-kestose + D-glucose

W8GV60

Substrates: -
Products: -

2 sucrose

6-kestose + D-glucose

Substrates: -
Products: -

2 sucrose

6-kestose + D-glucose

Burkholderia cepacia

Substrates: -
Products: -

2 sucrose

6-kestose + D-glucose

Dactylis glomerata

Substrates: -
Products: -

2 sucrose

6-kestose + D-glucose

Substrates: -
Products: -

2 sucrose

6-kestose + D-glucose

Substrates: -
Products: -

2 sucrose

6-kestose + D-glucose

Substrates: -
Products: -

2 sucrose

6-kestose + D-glucose

Lactobacillus gasseri

Substrates: -
Products: -

2 sucrose

6-kestose + D-glucose

Substrates: -
Products: -

2 sucrose

6-kestose + D-glucose

Substrates: -
Products: -

2 sucrose

6-kestose + D-glucose

Phleum pratense

Substrates: -
Products: -

2 sucrose

6-kestose + D-glucose

Substrates: -
Products: -

2 sucrose

6-kestose + D-glucose

Substrates: -
Products: -

2 sucrose

6-kestose + D-glucose

O68609

Substrates: -
Products: -

2 sucrose

6-kestose + D-glucose

Substrates: -
Products: -

2 sucrose

6-kestose + D-glucose

Substrates: -
Products: -

2 sucrose

6-kestose + D-glucose

Substrates: -
Products: -

2 sucrose

D-glucose + beta-D-fructofuranosyl-(2,6)-beta-D-fructofuranosyl-(2,1)-alpha-D-glucopyranoside

Substrates: -
Products: -

2 sucrose

D-glucose + beta-D-fructofuranosyl-(2,6)-beta-D-fructofuranosyl-(2,1)-alpha-D-glucopyranoside

Substrates: -
Products: -

alpha-D-galactopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-galactopyranosyl-1,2-beta-D-fructofuranoside

Substrates: besides the major product, levan, the enzyme produces two galactosyloligofructosides with D-Gal-Fru
Products: -

alpha-D-galactopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-galactopyranosyl-1,2-beta-D-fructofuranoside

Limosilactobacillus reuteri 21

Substrates: besides the major product, levan, the enzyme produces two galactosyloligofructosides with D-Gal-Fru
Products: -

alpha-D-mannopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-mannopyranosyl-1,2-beta-D-fructofuranoside

Substrates: only one mannosyloligofructoside is formed
Products: -

alpha-D-mannopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-mannopyranosyl-1,2-beta-D-fructofuranoside

Limosilactobacillus reuteri 21

Substrates: only one mannosyloligofructoside is formed
Products: -

alpha-D-xylopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-xylopyranosyl-1,2-beta-D-fructofuranoside

Substrates: besides the major product, levan, the enzyme produces at least three different xylosyloligofructosides with D-Xyl-Fru
Products: -

alpha-D-xylopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-xylopyranosyl-1,2-beta-D-fructofuranoside

Limosilactobacillus reuteri 21

Substrates: besides the major product, levan, the enzyme produces at least three different xylosyloligofructosides with D-Xyl-Fru
Products: -

inulin + H2O

Substrates: -
Products: -

inulin + H2O

Substrates: -
Products: -

jaggary

D-glucose + fructose + kestose + nystose + difructose + levan

Substrates: -
Products: -

jaggary

D-glucose + fructose + kestose + nystose + difructose + levan

Leuconostoc mesenteroides MTCC10508

Substrates: -
Products: -

raffinose + (2,6-beta-D-fructosyl)n

galactose + (2,6-beta-D-fructosyl)n+1

488316

Substrates: -
Products: the levan synthesized on raffinose contains one mol of galactosylglucose per mol as one of the 2 terminal glycosyl moieties

raffinose + (2,6-beta-D-fructosyl)n

galactose + (2,6-beta-D-fructosyl)n+1

488324

Substrates: in 0.38 M phosphate buffer
Products: -

raffinose + (2,6-beta-D-fructosyl)n

galactose + (2,6-beta-D-fructosyl)n+1

Substrates: -
Products: -

sucrose

levan + fructooligosaccharides

Substrates: the reaction occurs only in the presence of high salt concentrations (more than 1.5 M NaCl)
Products: -

sucrose

levan + fructooligosaccharides

Substrates: the reaction occurs only in the presence of high salt concentrations (more than 1.5 M NaCl)
Products: -

sucrose

levan + kestose

675837

Substrates: at concentrations below 85 mM (at 37°C). Above 85 mM sucrose, the transglycosylation activity of Lev gradually becomes significant. Transglycosylation never exceeds 50% of total enzyme activity
Products: -

sucrose

levan + kestose

Limosilactobacillus reuteri 121

675837

sucrose + (2,6-beta-D-fructosyl)n

alpha-D-glucose + (2,6-beta-D-fructosyl)n+1

Substrates: -
Products: -

sucrose + (2,6-beta-D-fructosyl)n

alpha-D-glucose + (2,6-beta-D-fructosyl)n+1

Substrates: capacity to produce extracellular homopolysaccharides of fructose, modulation of the composition of the biofilms that form on teeth
Products: -

sucrose + (2,6-beta-D-fructosyl)n

alpha-D-glucose + (2,6-beta-D-fructosyl)n+1

Substrates: -
Products: -

sucrose + (2,6-beta-D-fructosyl)n

alpha-D-glucose + (2,6-beta-D-fructosyl)n+1

Substrates: capacity to produce extracellular homopolysaccharides of fructose, modulation of the composition of the biofilms that form on teeth
Products: -

sucrose + (2,6-beta-D-fructosyl)n

D-glucose + (2,6-beta-D-fructosyl)n+1

685846

Substrates: -
Products: -

sucrose + (2,6-beta-D-fructosyl)n

D-glucose + (2,6-beta-D-fructosyl)n+1

689722

Substrates: reduction of glucose by yeasts (Candida cacaoi DSM 2226), introduced into a dialysing membrane, situated in the reaction medium, and the presence of Mn2+ results in an increase of levan synthesis efficiency to 64%
Products: -

sucrose + (2,6-beta-D-fructosyl)n

D-glucose + (2,6-beta-D-fructosyl)n+1

Substrates: -
Products: -

sucrose + (2,6-beta-D-fructosyl)n

D-glucose + (2,6-beta-D-fructosyl)n+1

Substrates: polyfructan produced by the levansucrase from Bacillus megaterium has a molecular mass of 2711 kDa and consisted mainly of beta(2,6) linkages. Besides the polyfructan formation, the wild-type levansucrase of Bacillus megaterium also synthesizes five different detectable oligosaccharides. Three products are identified: 1-kestose (isokestose), 6-kestose and nystose which are known acceptors for the transfer of fructosyl units
Products: -

sucrose + (2,6-beta-D-fructosyl)n

D-glucose + (2,6-beta-D-fructosyl)n+1

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Substrates: reverse reaction is probably catalyzed by a different levanase
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Substrates: enzyme is probably involved in initiation and progression of dental caries and periodontal diseases
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Substrates: reverse reaction is probably catalyzed by a different levanase
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Substrates: enzyme is probably involved in initiation and progression of dental caries and periodontal diseases
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Substrates: -
Products: high molecular weight branched levan

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Substrates: -
Products: product forms a complex with the enzyme

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Aerobacter levanicum

Substrates: -
Products: product forms a complex with the enzyme

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Aerobacter levanicum

Substrates: levan biosynthesis
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Substrates: -
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Bacillus sp. (in: Bacteria)

Substrates: activity is affected by sacU mutation
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

488329

Substrates: -
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Bacillus sp. (in: Bacteria) TH4-2

488329

Substrates: -
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

488310, 488316, 488317, 488318, 488319, 488320, 488321, 488338

Substrates: -
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

488311, 488315, 488330

Substrates: -
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Substrates: polymerase and hydrolase activity can be separately modulated by site-directed mutagenesis
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

488332

Substrates: reaction kinetics are dependent on sucrose concentration
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Substrates: activity is affected by sacU mutation
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Bacillus subtilis BS5C4

488315

Substrates: -
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Bacillus subtilis QB112

488310

Substrates: -
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Bacillus subtilis QB127

Substrates: -
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Bacillus subtilis QB127

Substrates: activity is affected by sacU mutation
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

488327, 488339

Substrates: -
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Substrates: various acceptors
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Substrates: beta-D-fructofuranosidase activity
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Substrates: synthesis of a high-molecular-mass fructan of the levan type
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Substrates: translocation of the enzyme is a rate-limiting step
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

488333, 488336, 488337

Substrates: -
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

488337

Substrates: additional beta-fructosidase, i.e. invertase, sucrose-sucrose 1-fructosyltransferase, and sucrose-sucrose 6-fructosyltransferase activities of recombinant enzyme from Pichia pastoris
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Substrates: various acceptors, e.g. fructosyl acceptors 2-1 linked or 2-6 linked, including water, the latter belongs to a fructosidase, i.e. invertase, activity of the purified enzyme, overview
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

488333, 488336

Substrates: transgenic plants
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

488333

Substrates: biosynthesis of fructans, important in assimilate partitioning and possibly in stress tolerance in plants
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Substrates: key enzyme for the formation of the graminans and phleins
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Leuconostoc mesenteroides B-512 FMC

672335

Substrates: -
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

672335

Substrates: -
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Substrates: hydrolase, i.e. invertase, and transferase activities can be altered by modifying reaction conditions
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Pantoea agglomerans

488309

Substrates: beta-D-fructofuranosidase activity
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

488324

Substrates: -
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Substrates: -
Products: molecular weight of levan product is dependent on temperature, salinity, and sucrose concentration

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

488326, 488328

Substrates: -
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Substrates: various D-fructosyl acceptors, overview
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Substrates: transfucosylation from sucrose to various acceptors, except sugar alcohols
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Rahnella aquatilis ATCC 15552

Substrates: the degree of polymerization of levan produced at high temperature is lower than that produced at low temperature
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

488326

Substrates: -
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Substrates: transfucosylation from sucrose to various acceptors, except sugar alcohols
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Substrates: the degree of polymerization of levan produced at high temperature is lower than that produced at low temperature
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Substrates: various D-fructosyl acceptors, overview
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Streptococcus mutans

488323, 488330

Substrates: -
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

488314, 488331, 488343

Substrates: -
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Substrates: -
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Q60114

488341

Substrates: -
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Substrates: -
Products: enzyme plays an important role in the production of ethanol from sugar cane and other sucrose sources

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Q60114

488344

Substrates: -
Products: molecular weight of produced levan is dependent on preparation method, immobilized recombinant enzyme and recombinant enzyme in permeabilized E. coli cells produces low molecular weight levan, free recombinant enzyme produces high molecular weight levan

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Substrates: at lower temperatures, such as 5°C and 15°C and pH 4.0-6.0 the transfructosylation is preferentially catalyzed rather than the hydrolysis of sucrose, but inversely at higher temperatures such as 30°C and 40°C and pH 7.0-8.0 the hydrolysis is preferentially catalyzed
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Substrates: enzyme catalyzes the liberation of reducing sugars from substrates having 2-beta-D-fructofuranose residues on a terminal such as sucrose, raffinose, levan from Aerobacter levanicum and inulin with a relative activity of 100:104:1:0.01
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Zymomonas mobilis ZM1

Q60114

488341

Substrates: -
Products: -

sucrose + ?

blastose + ?

Substrates: -
Products: two-dimensional COSY, TOCSY, HMBC and HSQC experiments

sucrose + ?

blastose + ?

Substrates: -
Products: two-dimensional COSY, TOCSY, HMBC and HSQC experiments

sucrose + ?

polyfructan + ?

Substrates: -
Products: polyfructan, molecular mass of 2711 kDa and consisted mainly of beta(2-6) linkages

sucrose + ?

polyfructan + ?

Substrates: -
Products: polyfructan, molecular mass of 2711 kDa and consisted mainly of beta(2-6) linkages

sucrose + acarbose

D-glucose + 1'-beta-D-fructofuranosyl alpha-acarbose

Leuconostoc mesenteroides B-512 FMC

703493

Substrates: -
Products: product analysis and structure determination by NMR spectroscopy

sucrose + acarbose

D-glucose + 1'-beta-D-fructofuranosyl alpha-acarbose

703493

Substrates: -
Products: product analysis and structure determination by NMR spectroscopy

sucrose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n

D-glucose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n+1

Substrates: -
Products: -

sucrose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n

D-glucose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n+1

Bacillus velezensis SK 21.002

Substrates: -
Products: -

sucrose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n

D-glucose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n+1

Substrates: -
Products: -

sucrose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n

D-glucose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n+1

Substrates: -
Products: -

sucrose + alpha-D-glucosyl-(1->2)-[(2->6)-beta-D-fructosyl]n

D-glucose + alpha-D-glucosyl-(1->2)-[(2->6)-beta-D-fructosyl]n+1

Substrates: -
Products: -

sucrose + alpha-D-glucosyl-(1->2)-[(2->6)-beta-D-fructosyl]n

D-glucose + alpha-D-glucosyl-(1->2)-[(2->6)-beta-D-fructosyl]n+1

Substrates: -
Products: -

sucrose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n alpha-D-glucopyranoside

D-glucose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n alpha-D-glucopyranoside

D-glucose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n alpha-D-glucopyranoside

D-glucose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n+1 alpha-D-glucopyranoside

Substrates: the highest product molecular weight of 38 kDa is reached at 30°C, and gradually decreases at higher temperatures
Products: -

sucrose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n alpha-D-glucopyranoside

D-glucose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n alpha-D-glucopyranoside

D-glucose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n+1 alpha-D-glucopyranoside

Substrates: sucrose is used as a starter unit to which fructose residues, cleaved from another sucrose molecule, become attached
Products: -

sucrose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n alpha-D-glucopyranoside

D-glucose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n alpha-D-glucopyranoside

D-glucose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n+1 alpha-D-glucopyranoside

Substrates: sucrose is used as a starter unit to which fructose residues, cleaved from another sucrose molecule, become attached
Products: -

sucrose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n alpha-D-glucopyranoside

D-glucose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n+1 alpha-D-glucopyranoside

701837

Substrates: -
Products: -

sucrose + cellobiose

beta-D-glucopyranosyl-(1,4)-alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranoside + D-glucose

Substrates: -
Products: -

sucrose + cellobiose

beta-D-glucopyranosyl-(1,4)-alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranoside + D-glucose

Substrates: -
Products: -

sucrose + cellobiose

D-glucose + beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside

Substrates: -
Products: the major transfructosylation product is identified to be cellobiose-fructose (beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside)

sucrose + cellobiose

D-glucose + beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside

Substrates: -
Products: -

sucrose + cellobiose

D-glucose + beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside

Substrates: -
Products: -

sucrose + cellobiose

fructosylated cellobiose + ?

Substrates: -
Products: -

sucrose + cellobiose

fructosylated cellobiose + ?

Substrates: -
Products: -

sucrose + D-fucose

beta-D-fructofuranosyl-alpha-D-fucopyranoside + D-glucose

Substrates: -
Products: -

sucrose + D-fucose

beta-D-fructofuranosyl-alpha-D-fucopyranoside + D-glucose

Substrates: -
Products: -

sucrose + D-galactose

beta-D-fructofuranosyl-alpha-D-galactopyranoside + D-glucose

Substrates: -
Products: -

sucrose + D-galactose

beta-D-fructofuranosyl-alpha-D-galactopyranoside + D-glucose

Substrates: -
Products: -

sucrose + D-glucose

glucose + sucrose

Substrates: exchange reaction
Products: -

sucrose + D-glucose

glucose + sucrose

Substrates: exchange reaction
Products: -

sucrose + D-maltose

Substrates: -
Products: -

sucrose + D-maltose

Substrates: -
Products: -

sucrose + D-maltose

Substrates: -
Products: -

sucrose + D-maltose

Substrates: -
Products: -

sucrose + D-sorbitol

Substrates: low activity
Products: -

sucrose + D-sorbitol

Substrates: -
Products: -

sucrose + D-sorbitol

Substrates: -
Products: -

sucrose + D-xylose

Substrates: -
Products: -

sucrose + D-xylose

Leuconostoc mesenteroides MTCC10508

Substrates: -
Products: -

sucrose + D-xylose

Substrates: -
Products: -

sucrose + D-xylose

Substrates: -
Products: -

sucrose + D-xylose

beta-D-fructofuranosyl-alpha-D-xylopyranoside + D-glucose

Substrates: -
Products: -

sucrose + D-xylose

beta-D-fructofuranosyl-alpha-D-xylopyranoside + D-glucose

Substrates: -
Products: -

sucrose + D-xylose

D-glucose + alpha-D-xylopyranosyl-(1->2)-beta-D-fructofuranoside

Substrates: -
Products: -

sucrose + D-xylose

D-glucose + alpha-D-xylopyranosyl-(1->2)-beta-D-fructofuranoside

Substrates: -
Products: -

sucrose + galactose

Fru(beta-2,6)Gal + ?

Substrates: -
Products: -

sucrose + galactose

Fru(beta-2,6)Gal + ?

Substrates: -
Products: -

sucrose + glucose

1-kestotriose + 6-kestotriose + 1-kestotetraose + ?

Substrates: -
Products: -

sucrose + glucose

1-kestotriose + 6-kestotriose + 1-kestotetraose + ?

Substrates: -
Products: -

sucrose + H2O

D-glucose + D-fructose

Substrates: -
Products: -

sucrose + H2O

D-glucose + D-fructose

Substrates: hydrolysis
Products: -

sucrose + H2O

D-glucose + D-fructose

Substrates: -
Products: -

sucrose + H2O

D-glucose + D-fructose

Substrates: -
Products: -

sucrose + H2O

D-glucose + D-fructose

Clostridium arbusti

Clostridium arbusti SL206

Substrates: -
Products: -

sucrose + H2O

D-glucose + D-fructose

Substrates: -
Products: -

sucrose + H2O

D-glucose + D-fructose

Substrates: -
Products: -

sucrose + H2O

D-glucose + D-fructose

Substrates: -
Products: -

sucrose + H2O

D-glucose + D-fructose

Substrates: -
Products: -

sucrose + H2O

D-glucose + D-fructose

Substrates: -
Products: -

sucrose + H2O

D-glucose + D-fructose

Substrates: hydrolysis
Products: -

sucrose + hydroquinone

D-glucose + 4-hydroxyphenyl-beta-D-fructofuranoside

Leuconostoc mesenteroides B-512 FMC

701838

Substrates: -
Products: NMR product analysis, the product shows anti-oxidation activities and inhibition of tyrosinase and of 1,1-diphenyl-2-picrylhydrazyl scavenging activity, it also shows inhibition of lipid peroxidation, kinetics, overview

sucrose + hydroquinone

D-glucose + 4-hydroxyphenyl-beta-D-fructofuranoside

701838

sucrose + isomaltose

D-glucose + theanderose

Bacillus subtilis ATCC 6051

757034

Substrates: -
Products: -

sucrose + isomaltose

D-glucose + theanderose

757034

Substrates: -
Products: -

sucrose + isomaltose

D-glucose + theanderose

Bacillus subtilis CECT 3

757034

Substrates: -
Products: -

sucrose + lactose

Substrates: -
Products: -

sucrose + lactose

Leuconostoc mesenteroides MTCC10508

Substrates: -
Products: -

sucrose + lactose

Substrates: -
Products: -

sucrose + lactose

Substrates: -
Products: -

sucrose + lactose

Substrates: -
Products: -

sucrose + lactose

D-glucose + beta-D-galactopyranosyl-(1->4)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside

Substrates: -
Products: -

sucrose + lactose

D-glucose + beta-D-galactopyranosyl-(1->4)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside

Substrates: -
Products: -

sucrose + lactose

D-glucose + beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside

Substrates: -
Products: the major transfructosylation product is identified to be lactosefructose or lactosucrose (beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside)

sucrose + lactose

D-glucose + beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside

Leuconostoc mesenteroides B?512

736295

Substrates: optimized conditions are pH 6.5, 50°C, 27% w/v sucrose, 27% w/v lactose, and 5 U/ml of the purified recombinant enzyme
Products: i.e. lactosefructose or lactosucrose

sucrose + lactose

D-glucose + beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside

736295

Substrates: optimized conditions are pH 6.5, 50°C, 27% w/v sucrose, 27% w/v lactose, and 5 U/ml of the purified recombinant enzyme
Products: i.e. lactosefructose or lactosucrose

sucrose + lactose

D-glucose + lactosylfructoside

735894

Substrates: method optimization for lactosylfructoside biosynthesis, overview. Equal concentration of the two substrates showed a better performance than the other concentration ratios
Products: lactosylfructoside is a functional oligosaccharide consisting of D-glucose, D-galactose, and D-fructose. Purification of the trisaccharide from the product using a HPLC system and confirmation by NMR spectroscopy

sucrose + lactose

D-glucose + lactosylfructoside

Bacillus velezensis SK 21.002

735894

sucrose + lactose

D-glucose + O-beta-D-galactopyranosyl-(1,4)-O-beta-D-glucopyranosyl-(1,2)-beta-D-fructofuranoside

Substrates: levansucrase transfers the fructosyl moiety of sucrose to lactose to form lactosucrose
Products: i.e. lactosucrose or 4G-beta-D-galactosylsucrose

sucrose + lactose

D-glucose + O-beta-D-galactopyranosyl-(1,4)-O-beta-D-glucopyranosyl-(1,2)-beta-D-fructofuranoside

Substrates: levansucrase transfers the fructosyl moiety of sucrose to lactose to form lactosucrose, optimization of reaction conditions, 28.5% conversion in absence and 43.2% in presence of glucose oxidase, which eliminates the inhibitory D-glucose reaction product, overview
Products: i.e. lactosucrose or 4G-beta-D-galactosylsucrose, product identification and quantification by NMR spectroscopy

sucrose + lactose

lactosucrose

Bacillus amyloliquefaciens IFO15535

Substrates: -
Products: -

sucrose + lactose

lactosucrose

Substrates: -
Products: -

sucrose + lactose

lactosucrose

Geobacillus stearothermophilus

Substrates: Bacillus subtilis is the most effective producer of lactofructose
Products: -

sucrose + lactose

lactosucrose

Geobacillus stearothermophilus ATCC 12980

Substrates: -
Products: -

sucrose + lactose

lactosucrose

Substrates: -
Products: -

sucrose + lactose

lactosucrose

Paenibacillus polymyxa

Paenibacillus polymyxa KCCM35411

Substrates: -
Products: -

sucrose + lactose

lactosucrose

Substrates: -
Products: -

sucrose + lactose

lactosucrose

Pseudomonas syringae IFO14086

Substrates: -
Products: -

sucrose + lactose

lactosucrose

Substrates: -
Products: -

sucrose + lactose

lactosucrose

Rahnella aquatilis KCTC2858

Substrates: -
Products: -

sucrose + lactose

lactosucrose

Substrates: -
Products: -

sucrose + lactose

lactosucrose

Sterigmatomyces elviae

Sterigmatomyces elviae ATCC 18894

Substrates: -
Products: -

sucrose + lactose

lactosucrose

Substrates: -
Products: -

sucrose + maltose

Substrates: -
Products: -

sucrose + maltose

Leuconostoc mesenteroides MTCC10508

Substrates: -
Products: -

sucrose + maltose

Substrates: -
Products: -

sucrose + maltose

D-glucose + alpha-D-glucopyranosyl-(1->4)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside

Substrates: -
Products: -

sucrose + maltose

D-glucose + alpha-D-glucopyranosyl-(1->4)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside

Substrates: -
Products: -

sucrose + melibiose

D-glucose + raffinose

Substrates: -
Products: -

sucrose + melibiose

D-glucose + raffinose

Clostridium arbusti

Clostridium arbusti SL206

Substrates: when 30% (w/v) sucrose and 30% (w/v) melibiose are catalyzed using 3% (w/v) whole cells at pH 6.5 (50 mM sodium phosphate buffer) and 55°C, the highest raffinose yield is 222 g/l after a 6 h reaction. The conversion ratio from each substrate to raffinose is 50%
Products: -

sucrose + melibiose

D-glucose + raffinose

sucrose + melibiose

raffinose + D-glucose

Substrates: -
Products: -

sucrose + melibiose

raffinose + D-glucose

Substrates: -
Products: -

sucrose + methanol

glucose + methyl-beta-D-fructoside

Substrates: -
Products: -

sucrose + methanol

glucose + methyl-beta-D-fructoside

488328

Substrates: -
Products: -

sucrose + sorbitol

Substrates: -
Products: -

sucrose + sorbitol

Leuconostoc mesenteroides MTCC10508

Substrates: -
Products: -

sucrose + sucrose

1-kestose + 6-kestose + neo-kestose + ?

Substrates: -
Products: -

sucrose + sucrose

1-kestose + 6-kestose + neo-kestose + ?

Substrates: -
Products: -

sucrose + sucrose

1-kestose + 6-kestose + neo-kestose + ?

Substrates: -
Products: -

sucrose + sucrose

1-kestose + 6-kestose + neo-kestose + ?

Substrates: -
Products: -

sucrose + sucrose

1-kestose + 6-kestose + neo-kestose + ?

Substrates: -
Products: -

sucrose + sucrose

1-kestose + 6-kestose + neo-kestose + ?

Substrates: -
Products: -

sucrose + sucrose

6-kestose + levanbiose + blastose + 1-kestose + ?

Substrates: -
Products: -

sucrose + sucrose

6-kestose + levanbiose + blastose + 1-kestose + ?

Substrates: -
Products: -

sucrose + sucrose

Substrates: -
Products: -

sucrose + sucrose

Substrates: product concentrations of fructose, kestose or fructan depend on the concentration of sucrose, pH and temperatur
Products: -

sucrose + sucrose

D-glucose + 1-kestose

Substrates: -
Products: identified by HPAEC

sucrose + sucrose

D-glucose + 1-kestose

Substrates: -
Products: identified by HPAEC

sucrose + sucrose

D-glucose + 1-kestose + nystose + ?

Substrates: -
Products: -

sucrose + sucrose

D-glucose + 1-kestose + nystose + ?

Substrates: -
Products: -

sucrose + sucrose

D-glucose + 6-kestose

Substrates: -
Products: identified by HPAEC

sucrose + sucrose

D-glucose + 6-kestose

Substrates: -
Products: identified by HPAEC

sucrose + sucrose

D-glucose + 6-kestose + 6-nystose + 6,6,6-kestopentaose + levanbiose + levantriose + ?

Substrates: -
Products: -

sucrose + sucrose

D-glucose + 6-kestose + 6-nystose + 6,6,6-kestopentaose + levanbiose + levantriose + ?

Substrates: -
Products: -

sucrose + sucrose

D-glucose + 6-kestose + 6-nystose + 6,6,6-kestopentaose + levanbiose + levantriose + ?

Substrates: -
Products: -

sucrose + sucrose

D-glucose + levan

Substrates: -
Products: -

sucrose + sucrose

D-glucose + levan

Aspergillus awamori EM66

Substrates: -
Products: -

sucrose + sucrose

D-glucose + levan

Substrates: -
Products: -

sucrose + sucrose

D-glucose + levan

Bacillus subtilis NRC 16

Substrates: -
Products: -

sucrose + sucrose

D-glucose + levan

Substrates: -
Products: -

sucrose + sucrose

D-glucose + levan

Substrates: -
Products: -

sucrose + sucrose

D-glucose + levan

Substrates: -
Products: -

sucrose + sucrose

D-glucose + levan

756945

Substrates: -
Products: -

sucrose + sucrose

D-glucose + levan

Substrates: the enzyme has high product specificity, and no fructooligosaccharide is identified in the product
Products: -

sucrose + sucrose

D-glucose + levan

Substrates: -
Products: -

sucrose + sucrose

D-glucose + levan

Substrates: -
Products: -

sucrose + sucrose

D-glucose + levan

Substrates: -
Products: -

sucrose + sucrose

D-glucose + levan

Substrates: -
Products: -

sucrose + sucrose

D-glucose + levan

Substrates: -
Products: -

sucrose + sucrose

D-glucose + levan

Substrates: -
Products: -

sucrose + sucrose

D-glucose + levan

Substrates: -
Products: -

sucrose + sucrose

D-glucose + levan

Substrates: the recombinant enzyme shows 80% conversion yield
Products: -

sucrose + sucrose

D-glucose + levan

Substrates: the recombinant enzyme shows 80% conversion yield
Products: -

sucrose + sucrose

D-glucose + levan

Substrates: -
Products: -

sucrose + sucrose

D-glucose + levan

Substrates: -
Products: -

sucrose + sucrose

D-glucose + levan

Substrates: -
Products: -

sucrose + sucrose

D-glucose + levan

684132

Substrates: is assayed in a standard reaction containing 1 ml 10% sucrose and 1 ml enzyme (0.01-0.05mg/ml) incubated at 15°C
Products: -

sucrose + sucrose

D-glucose + levan type-fructan

Substrates: -
Products: -

sucrose + sucrose

D-glucose + levan type-fructan

Bacillus amyloliquefaciens KK9

Substrates: -
Products: -

sucrose + sucrose

D-glucose + low-molecular weight levan + short-chain fructooligosaccharides (DP 3-6)

Substrates: -
Products: -

sucrose + sucrose

D-glucose + low-molecular weight levan + short-chain fructooligosaccharides (DP 3-6)

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Aspergillus awamori EM66

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Azotobacter chroococcum

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Bacillus amyloliquefaciens KK9

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Bacillus subtilis NRC 16

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Burkholderia vietnamiensis

756945

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

A4JNE0

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Erwinia amylovora ATCC 49946

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Gluconobacter albidus

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Kozakia baliensis

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Paraburkholderia graminis ATCC 700544

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

756347, 756610

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Zymomonas mobilis subsp. mobilis

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Zymomonas mobilis subsp. mobilis ATCC 31821

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

additional information

Aerobacter levanicum

Substrates: 6-beta-D-fructosyl)n is levan
Products: -

additional information

730222

Substrates: design and evaluation of a bi-enzymatic system: combined use of levansucrase from Bacillus amyloliquefaciens and endoinulinase from Aspergillus niger in a one-step reaction for the synthesis of fructooligosaccharides (FOS) and oligolevans using sucrose as the sole substrate, overview. At the initial stage of time course, short chain fructooligosaccharides, 1-kestose, nystose, and 1F-fructosylnystose are the major products, whilst 6-kestose, medium chain fructooligosaccharides (mcFOSs, levanohexaose, levanopentaose) and oligolevans become the dominant ones at the late stage. NMR analysis of levan, levanopentaose and levanohexaose, overview
Products: -

additional information

Substrates: levansucrase catalyzes simultaneously both the transfructosylation and the hydrolysis, which is regarded as the transfer of the fructosyl group to water, time courses for transfructosylation and hydrolysis reactions, overview. Measurement of sucrose hydrolysis releasing D-glucose and D-fructose. Disaccharides lactose, cellobiose and melibiose are used as acceptors, with high acceptor specificity towards lactose. Maple syrup is used as assay medium and sucrose source, profile of the end-products synthesized by the enzyme, overview
Products: -

additional information

Bacillus amyloliquefaciens ATCC 23350

additional information

730222

additional information

Substrates: levansucrase catalyzes three distinct reactions depending on the fructosyl acceptor molecule, including polymerization, transfructosylation, and hydrolysis. Wide substrate specificity of levansucrase toward monosaccharides, disaccharides, and aromatic and alkyl alcohols, producing diverse sucrose analogues, hetero-oligosaccharides (especially lactosucrose), and fructosides, overview
Products: -

additional information

Substrates: broad transglycosylation capability of the levansucrase from Bacillus licheniformis strain 8-37-0-1, substrate specificity, overview. When using sucrose as the donor, the enzyme displays a wide range of acceptor specificity and is able to transfer fructosyl to a series of sugar acceptors including hexose, pentose, beta- or alpha-disaccharides, along with the difficult branched alcohols. Product analysis by mass and NMR spectrometry
Products: -

additional information

W8GV60

Substrates: levan synthesis, the first reaction of levan synthesis is formation of 6-kestose from two molecules of sucrose, one acting as a fructosyl donor and the other as an acceptor. 6-Kestose is further extended through numerous transfructosylation reactions
Products: -

additional information

Substrates: levansucrase catalyzes three distinct reactions depending on the fructosyl acceptor molecule: polymerization (using the growing fructan chain as an acceptor), transfructosylation (using monosaccharides, disaccharides, or oligosaccharides as acceptors), and hydrolysis (using water as an acceptor), overview
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additional information

Substrates: the enzyme also hydrolyzes sucrose to D-glucose and D-fructose
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additional information

additional information

Substrates: the enzyme also hydrolyzes sucrose to D-glucose and D-fructose
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additional information

Substrates: mutant enzymes Y429N and R433A no longer produce levan but exclusively oligosaccharides
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additional information

Substrates: mutant enzymes Y429N and R433A no longer produce levan but exclusively oligosaccharides
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additional information

Substrates: levansucrase catalyzes the synthesis of levan from sucrose, but it may also transfer the fructosyl moiety from sucrose to acceptor molecules included in the reaction medium
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additional information

Substrates: levansucrase catalyzes the synthesis of levan from sucrose, but it may also transfer the fructosyl moiety from sucrose to acceptor molecules. The wild-type enzyme synthesizes levan with molecular weight bimodal distribution, while the mutants R360K and Y429N synthesize only oligosaccharides, substrate specificities, overview
Products: -

additional information

Substrates: levan synthesis from sucrose, structure analysis by NMR spectrometry, overview. Levan size analysis by gel filtration. As sucrose concentration increases, the average molecular weight of the low dextran distribution decreases, at 4°C compared to 37°C, the low molecular weight distribution region increases in size at any given substrate concentration, and a maximum of 20.64 kDa is obtained at 200 g/l
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additional information

additional information

additional information

additional information

Substrates: measurement of sucrose hydrolysis, releasing D-glucose and D-fructose. As the reaction proceeds, SacB becomes less hydrolytic while transferase becomes preponderant to reach up to 80% at the end of the reaction
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additional information

additional information

additional information

Substrates: the enzyme also hydrolyzes sucrose to D-glucose and D-fructose
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additional information

Substrates: the enzyme also hydrolyzes sucrose to D-glucose and D-fructose
Products: -

additional information

Brenneria goodwinii

A0A0G4JRN2

755827

Substrates: the enzyme also hydrolyzes sucrose to D-glucose and D-fructose
Products: -

additional information

Brenneria goodwinii

755827

Substrates: the enzyme also hydrolyzes sucrose to D-glucose and D-fructose
Products: -

additional information

Burkholderia cepacia

additional information

Dactylis glomerata

additional information

additional information

Substrates: the enzyme also hydrolyzes sucrose to D-glucose and D-fructose
Products: -

additional information

Substrates: the enzyme also hydrolyzes sucrose to D-glucose and D-fructose
Products: -

additional information

Substrates: the enzyme also hydrolyzes sucrose to D-glucose and D-fructose
Products: -

additional information

Substrates: in Lactobacillus sanfranciscensis TMW 1.392, sucrose metabolism and formation of fructan and 1-kestose is dependent on the activity of a single enzyme, levansucrase
Products: -

additional information

additional information

Fructilactobacillus sanfranciscensis TMW 1.392

Substrates: in Lactobacillus sanfranciscensis TMW 1.392, sucrose metabolism and formation of fructan and 1-kestose is dependent on the activity of a single enzyme, levansucrase
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additional information

additional information

Substrates: the enzyme also hydrolyzes sucrose to D-glucose and D-fructose
Products: -

additional information

Substrates: the enzyme also hydrolyzes sucrose to D-glucose and D-fructose
Products: -

additional information

Substrates: the enzyme cannot hydrolyze levan from Halomonas smyrnensis, Zymomonas mobilis and Bacillus subtilis, and inulin from chicory
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additional information

Substrates: the enzyme cannot hydrolyze levan from Halomonas smyrnensis, Zymomonas mobilis and Bacillus subtilis, and inulin from chicory
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additional information

Substrates: the enzyme cannot hydrolyze levan from Halomonas smyrnensis, Zymomonas mobilis and Bacillus subtilis, and inulin from chicory
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additional information

Lactobacillus gasseri

additional information

Substrates: levan synthesis from sucrose
Products: -

additional information

Substrates: measurement of sucrose hydrolysis
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additional information

Substrates: levan synthesis from sucrose
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additional information

Substrates: measurement of sucrose hydrolysis
Products: -

additional information

additional information

Microbacterium laevaniformans

additional information

Microbacterium laevaniformans

additional information

additional information

Substrates: the enzyme also hydrolyzes sucrose to D-glucose and D-fructose
Products: -

additional information

Substrates: the enzyme also hydrolyzes sucrose to D-glucose and D-fructose
Products: -

additional information

Substrates: levan, a polymer of fructose linked by fructofurano side bonds, is produced by the transfructosylation reaction of levansucrase
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additional information

Substrates: the enzyme also hydrolyzes sucrose to D-glucose and D-fructose
Products: -

additional information

Substrates: the enzyme also shows hydrolytic activity with sucrose producing D-glucose and D-fructose
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additional information

Phleum pratense

additional information

additional information

additional information

675845

Substrates: the product levan has not a major impact on biofilm formation
Products: -

additional information

673743

Substrates: thermo-responsive expression and differential secretion of the enzyme. The extracellular levansucrase is encoded by two functional genes, lscB and lscC. Transcription of lscB and lscC is temperature-dependent. Quantification of Lsc in supernatants and cellular protein samples of mutants defective in either lscB or lscC confirm that LscB secretion at low temperature is due to a combination of thermo-regulated transcription and secretion. LscC accumulates in the periplasmic space
Products: -

additional information

O68609

additional information

Substrates: isozyme Lsc3 synthesizes a high molecular weight fructan polymer, levan, from sucrose, but it also shows formation of fructooligosaccharides with potential prebiotic effects from both sucrose and raffinose, degrees of polymerization up to five, overview. Formation of fructooligosaccharides is enhanced by increased sucrose or raffinose concentrations of 600 mM and higher. Analysis of product in underivatized form by fully automated chip-based nanoelectrospray ionization, nanoESI, high-capacity ion trap mass spectrometry, HCT-MS. Structural analysis by tandem mass spectrometry, MS/MS, employing collision-induced dissociation at low energies, overview
Products: -

additional information

Substrates: assay of the growth phenotype of levansucrase-expressing bacteria on agar plate containing sucrose. Only wild-type Lsc3 produces levan giving a mucoid phenotype to the streaks of respective transformant. Quantitative evaluation of the sucrose-splitting (total) levansucrase activity on microplates
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additional information

additional information

Q883P5

additional information

additional information

Q883P5

additional information

additional information

additional information

additional information

Substrates: the enzyme also hydrolyzes sucrose to D-glucose and D-fructose
Products: -

additional information

Substrates: the enzyme also hydrolyzes sucrose to D-glucose and D-fructose
Products: -

additional information

Substrates: the enzyme also hydrolyzes sucrose to D-glucose and D-fructose
Products: -

additional information

Q60114

488344

Substrates: production of low molecular weight levan in permeabilized recombinant E. coli cells
Products: -

additional information

Substrates: 6-beta-D-fructosyl)n is levan
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additional information

Q60114

Substrates: change in substrate specificity appears to be unique to Zymomonas mobilis levansucrase because the levansucrases from Bacillus subtilis, Rahnella aquatilis, and Lactobacillus reuteri are capable of synthesizing levan in their soluble form and are not reported to form fibril-like structures
Products: -

additional information

additional information

Substrates: levan biosynthesis and fructooligosaccharide biosynthesis
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additional information

additional information

736648

Substrates: three activities are displayed by the levansucrase from Zymomonas mobilis: formation of levan (polymerization), production of short-chain fructooligosaccharides (FOS), and sucrose hydrolysis. Several inulin-type FOS (1-kestose, nystose, 1F-fructosylnystose), neo-FOS (blastose, neokestose, neonystose) and levan-type FOS (6-kestose, 6,6-nystose) are synthesized by levansucrase, identification by mass spectroscopy and NMR spectrometry, e.g. of 6,6-nystose (i.e. Fru-beta(2->6)-Fru-beta(2->6)-Fru-beta(2->1)-alphaGlc). Assay method optimization, transfructosylation to hydrolysis ratio. , overview
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additional information

Zymomonas mobilis 113S

Substrates: levan biosynthesis and fructooligosaccharide biosynthesis
Products: -

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NATURAL SUBSTRATE

NATURAL PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

LITERATURE

COMMENTARY

REVERSIBILITY
r=reversible
ir=irreversible
?=not specified

2 sucrose

D-glucose + beta-D-fructofuranosyl-(2,6)-beta-D-fructofuranosyl-(2,1)-alpha-D-glucopyranoside

sucrose + (2,6-beta-D-fructosyl)n

alpha-D-glucose + (2,6-beta-D-fructosyl)n+1

sucrose + (2,6-beta-D-fructosyl)n

D-glucose + (2,6-beta-D-fructosyl)n+1

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

14 entries

sucrose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n

D-glucose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n+1

4 entries

sucrose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n alpha-D-glucopyranoside

D-glucose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n+1 alpha-D-glucopyranoside

5 entries

sucrose + lactose

D-glucose + O-beta-D-galactopyranosyl-(1,4)-O-beta-D-glucopyranosyl-(1,2)-beta-D-fructofuranoside

Substrates: levansucrase transfers the fructosyl moiety of sucrose to lactose to form lactosucrose
Products: i.e. lactosucrose or 4G-beta-D-galactosylsucrose

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

41 entries

additional information

20 entries

2 sucrose

D-glucose + beta-D-fructofuranosyl-(2,6)-beta-D-fructofuranosyl-(2,1)-alpha-D-glucopyranoside

Substrates: -
Products: -

2 sucrose

D-glucose + beta-D-fructofuranosyl-(2,6)-beta-D-fructofuranosyl-(2,1)-alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + (2,6-beta-D-fructosyl)n

alpha-D-glucose + (2,6-beta-D-fructosyl)n+1

Substrates: capacity to produce extracellular homopolysaccharides of fructose, modulation of the composition of the biofilms that form on teeth
Products: -

sucrose + (2,6-beta-D-fructosyl)n

alpha-D-glucose + (2,6-beta-D-fructosyl)n+1

Substrates: capacity to produce extracellular homopolysaccharides of fructose, modulation of the composition of the biofilms that form on teeth
Products: -

sucrose + (2,6-beta-D-fructosyl)n

D-glucose + (2,6-beta-D-fructosyl)n+1

685846

Substrates: -
Products: -

sucrose + (2,6-beta-D-fructosyl)n

D-glucose + (2,6-beta-D-fructosyl)n+1

Substrates: -
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Substrates: enzyme is probably involved in initiation and progression of dental caries and periodontal diseases
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Substrates: enzyme is probably involved in initiation and progression of dental caries and periodontal diseases
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sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Aerobacter levanicum

Substrates: levan biosynthesis
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sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Substrates: activity is affected by sacU mutation
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Substrates: activity is affected by sacU mutation
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Bacillus subtilis QB127

Substrates: activity is affected by sacU mutation
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Substrates: synthesis of a high-molecular-mass fructan of the levan type
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Substrates: translocation of the enzyme is a rate-limiting step
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

488337

Substrates: -
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

488333, 488336

Substrates: transgenic plants
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

488333

Substrates: biosynthesis of fructans, important in assimilate partitioning and possibly in stress tolerance in plants
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sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Substrates: key enzyme for the formation of the graminans and phleins
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Streptococcus mutans

488323

Substrates: -
Products: -

sucrose + (2,6-beta-D-fructosyl)n

glucose + (2,6-beta-D-fructosyl)n+1

Substrates: -
Products: -

sucrose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n

D-glucose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n+1

Substrates: -
Products: -

sucrose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n

D-glucose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n+1

Bacillus velezensis SK 21.002

Substrates: -
Products: -

sucrose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n

D-glucose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n+1

Substrates: -
Products: -

sucrose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n

D-glucose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n+1

Substrates: -
Products: -

sucrose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n alpha-D-glucopyranoside

D-glucose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n alpha-D-glucopyranoside

D-glucose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n alpha-D-glucopyranoside

D-glucose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n alpha-D-glucopyranoside

D-glucose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n alpha-D-glucopyranoside

D-glucose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n+1 alpha-D-glucopyranoside

701837

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Aspergillus awamori EM66

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Azotobacter chroococcum

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Bacillus amyloliquefaciens KK9

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Bacillus subtilis NRC 16

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Burkholderia vietnamiensis

756945

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

A4JNE0

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Erwinia amylovora ATCC 49946

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Gluconobacter albidus

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Kozakia baliensis

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Paraburkholderia graminis ATCC 700544

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

756347, 756610

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Zymomonas mobilis subsp. mobilis

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Zymomonas mobilis subsp. mobilis ATCC 31821

Substrates: -
Products: -

sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside

D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside

Substrates: -
Products: -

additional information

Aerobacter levanicum

Substrates: 6-beta-D-fructosyl)n is levan
Products: -

additional information

additional information

Substrates: levansucrase catalyzes the synthesis of levan from sucrose, but it may also transfer the fructosyl moiety from sucrose to acceptor molecules included in the reaction medium
Products: -

additional information

additional information

additional information

additional information

Substrates: in Lactobacillus sanfranciscensis TMW 1.392, sucrose metabolism and formation of fructan and 1-kestose is dependent on the activity of a single enzyme, levansucrase
Products: -

additional information

Fructilactobacillus sanfranciscensis TMW 1.392

Substrates: in Lactobacillus sanfranciscensis TMW 1.392, sucrose metabolism and formation of fructan and 1-kestose is dependent on the activity of a single enzyme, levansucrase
Products: -

additional information

Substrates: levan synthesis from sucrose
Products: -

additional information

Microbacterium laevaniformans

Substrates: levan synthesis from sucrose
Products: -

additional information

additional information

Substrates: levan, a polymer of fructose linked by fructofurano side bonds, is produced by the transfructosylation reaction of levansucrase
Products: -

additional information

675845

Substrates: the product levan has not a major impact on biofilm formation
Products: -

additional information

673743

additional information

additional information

additional information

Q60114

488344

Substrates: production of low molecular weight levan in permeabilized recombinant E. coli cells
Products: -

additional information

Substrates: 6-beta-D-fructosyl)n is levan
Products: -

additional information

Substrates: levan biosynthesis and fructooligosaccharide biosynthesis
Products: -

additional information

Zymomonas mobilis 113S

Substrates: levan biosynthesis and fructooligosaccharide biosynthesis
Products: -

show all columns Go to Metals and Ions Search

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METALS and IONS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Ba2+

107% activity at 5 mM

Ca2+

8 entries

Co2+

Bacillus sp. (in: Bacteria)

Fe2+

4fold increase in activity at 5 mM

488329

Fe3+

dependent on for tertiary structure, can partially be replaced by Sr2+, not Mg2+, Ba2+, and Mn2+

488310

K+

118% activity at 5 mM

Mg2+

Mn2+

MnCl2

22% activation of the immobilized enzyme at 0.1 mM, 30% activation at 0.1 mM of the free enzyme

Ni2+

109% activity at 5 mM

Rb+

activates slightly, recombinant enzyme

Sn2+

143% activity at 5 mM

additional information

Ca2+

dependent on for tertiary structure, can partially be replaced by Sr2+, not Mg2+, Sr2+, Ba2+, and Mn2+

488310

Ca2+

activates both transfructosylation and hydrolytic activities

Ca2+

113% activity at 5 mM

Ca2+

activates, recombinant enzyme

Ca2+

addition of 0.1 mM Ca2+ in the reaction medium, containing 0.05 mM EDTA, causes a significant increase in the enzyme's activity, achieving about 100% relative activity

Ca2+

Mg2+, Zn2+, Mn2+, Fe2+ have no effect

Ca2+

Q8GGV4

Ca2+ play an important structural role. At 45°C the mutant enzymes D500A and D500N are inactive in the absence of Ca2+ ions, with, respectively, 15% and 45% of wild-type activity remaining in the presence of 1 mM Ca2+. In the presence of 1 mM Ca2+ mutant enzymes display highest activity at 40°C. In the absence of Ca2+ ions, the optimal temperature is 30°C

658750

Ca2+

Q8GGV4

stimulates activity, Asp500 residues play an important role in Ca2+ binding

658750

Co2+

slightly activating

Co2+

activates strongly, recombinant enzyme

Mg2+

activates both transfructosylation and hydrolytic activities

Mg2+

115% activity at 5 mM

Mn2+

The transferase activity of levansucrase in the reaction mixture supplemented with Mn2+ is 100% higher than the enzyme activity in medium without metal ions, the hydrolytic activity of the levansucrase is lowered by 80%

689722

Mn2+

128% activity at 5 mM

additional information

not influenced by Rb+, Na+, Co2+ and Li+

additional information

enzyme activities of free and immobilized enzymes are not affected by Ca2+

additional information

not affected by 5 mM of Ba2+, Zn2+, Hg2+, Ni2+, Mn2+, Cu2+, Co2+, Ca2+, Na2MoO4

show all columns Go to Inhibitor Search

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INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

2-mercaptoethanol

slight inhibition at 50 mM

6-phosphogluconate

reaction intermediate

Ag+

AgCl

immobilized enzyme: 38% inhibition at 0.1 mM, 67% inhibition at 1 mM, complete inhibition at 10 mM. Free enzyme: 49% inhibition at 0.1 mM, 71% inhibition at 1 mM, 95% inhibition at 10 mM

AgNO3

1 mM, 86% inhibition

Al3+

Ba2+

complete inhibition at 5 mM

Ca2+

Co2+

complete inhibition at 1 mM

Cr3+

42.4% residual activity at 0.02 mM

Cu2+

7 entries

CuSO4

immobilized enzyme: 25% inhibition at 0.1 mM, 37% inhibition at 1 mM, 65% inhibition at 10 mM. Free enzyme: 30% inhibition at 0.1 mM, 42% inhibition at 1 mM, 79% inhibition at 10 mM

D-glucose

dithiothreitol

EDTA

ethanol

complete inhibition at 1.6 M

Fe2(SO4)3

Fe2+

4 entries

Fe3+

20 ng/ml medium, 30% inhibition of the free enzyme, 22% inhibition of the immobilized enzyme

FeCl3

immobilized enzyme: 8% inhibition at 0.1 mM, 21% inhibition at 1 mM, 35% inhibition at 10 mM. Free enzyme: 13% inhibition at 0.1 mM, 29% inhibition at 1 mM, 40% inhibition at 10 mM

FeSO4

1 mM, 17% inhibition

Hg2+

HgCl2

immobilized enzyme: 54% inhibition at 0.1 mM, 61% inhibition at 1 mM, 79% inhibition at 10 mM. Free enzyme: 61% inhibition at 0.1 mM, 75% inhibition at 1 mM, 91% inhibition at 10 mM

high ionic strength

Iodine

slight inhibition

K+

Li+

mannose

Mersalyl acid

Mg2+

MgSO4

11% inhibition of the immobilized enzyme at 10 mM, 21% inhibition of the free enzyme at 10 mM

Mn2+

N-bromosuccinimide

Na+

Na2S2O5

NaBr

NaCl

NaNO3

Ni2+

phenylmethanesulfonyl fluoride

proteinase K

raffinose

SDS

sucrose

Triton X-100

71% residual activity at 5 mM

Urea

Zn2+

5 entries

additional information

7 entries

Ag+

2% residual activity at 5 mM

Ag+

Al3+

37.9% residual activity at 0.02 mM

Al3+

55% residual activity at 5 mM

Ca2+

46.1% residual activity at 0.02 mM

Ca2+

1% residual activity at 1 mM

Cu2+

52.2% residual activity at 0.02 mM

Cu2+

inhibits transfructosylation and hydrolytic activities

Cu2+

5% residual activity at 5 mM

Cu2+

complete inhibition at 1 mM

Cu2+

Cu2+

20 ng/ml medium, 42% inhibition of the free enzyme, 80% inhibition of the immobilized enzyme

Cu2+

D-glucose

D-glucose

D-glucose acts as an inhibitor of the transfructosylation reaction, the decrease of glucose concentration by yeast in the medium by 16-19% results in the increased degree of levan polymerisation (by 6 to 9%) and efficiency of levan synthesis (by 9 to 11%)

689722

D-glucose

complete inhibition at 30 mM

D-glucose

complete inhibition at 30 mM

488314, 488330

D-glucose

shows high product inhibition in lactosucrose formation, D-glucose is therefor eliminated from the reaction system of lactosucrose production by glucose oxidase

dithiothreitol

complete inhibition at 1 mM

dithiothreitol

complete inhibition at 1 mM

dithiothreitol

weak inhibition at 50 mM

EDTA

41.8% residual activity at 0.02 mM

EDTA

36% residual activity at 5 mM

EDTA

97% inhibition

EDTA

the presence of 0.05 mM EDTA results in a decrease in the enzymatic activity by about 80%

EDTA

incubation with EDTA blocks enzyme activity completely

Fe2+

inhibits transfructosylation and hydrolytic activities

Fe2+

50% residual activity at 5 mM

Fe2+

complete inhibition at 1 mM

Fe2+

strong inhibition

Hg2+

2% residual activity at 5 mM

Hg2+

strong

Hg2+

20 ng/ml medium, 67.5% inhibition of the free enzyme, 54.3% inhibition of the immobilized enzyme

Hg2+

Hg2+

no inhibition by HgCl2, 5 mM

high ionic strength

suppresses transfer of fructosyl residues, especially in synthesis of high molecular weight levan, suppresses hydrolysis of levan, effect occurs with almost the same degree in hydrolysis of both low and high molecular weight levans

488316

high ionic strength

under conditions of high ionic strength, only levan with an average degree of polymerization of 120 is synthesized with a reasonable yield

488320

K+

49.1% residual activity at 0.02 mM

K+

Li+

inactivation at 8 M

Li+

mannose

no inhibition

mannose

Mg2+

45.4% residual activity at 0.02 mM

Mg2+

61% residual activity at 5 mM

Mg2+

Mn2+

inhibits only the hydrolytic activity

Mn2+

6% residual activity at 5 mM

Mn2+

Mn2+

20 ng/ml medium, 80% inhibition of the free enzyme, 75% inhibition of the immobilized enzyme

Na+

54.5% residual activity at 0.02 mM

Na+

NaCl

immobilized enzyme: 20% inhibition at 0.1 mM, 29% inhibition at 1 mM, 50% inhibition at 10 mM. Free enzyme: 26% inhibition at 0.1 mM, 40% inhibition at 1 mM, 55% inhibition at 10 mM

NaCl

30% of the protein is precipitated at 2 M NaCl

Ni2+

inhibits transfructosylation and hydrolytic activities

Ni2+

complete inhibition at 1 mM

Ni2+

Ni2+

slight inhibition

proteinase K

90% remaining activity after incubation at 37°C for 1 h

proteinase K

proteinase K has no effect

raffinose

raffinose

SDS

immobilized enzyme: 11% inhibition at 0.1 mM, 21% inhibition at 1 mM, 48% inhibition at 10 mM. Free enzyme: 14% inhibition at 0.1 mM, 27% inhibition at 1 mM, 52% inhibition at 10 mM

SDS

3% residual activity at 5 mM

SDS

2% residual activity at 5 mM

sucrose

above 300 mM, inhibition of transferase activity, not hydrolase activity

sucrose

Q60114

substrate inhibition

Urea

weak inhibition at 5 M

Urea

6 M urea decreased the activity by 40%

Zn2+

33.9% residual activity at 0.02 mM

Zn2+

inhibits transfructosylation and hydrolytic activities

Zn2+

41% residual activity at 5 mM

Zn2+

Zn2+

20 ng/ml medium, 75% inhibition of the free enzyme, 70% inhibition of the immobilized enzyme

additional information

no inhibition by EDTA at 0.1-10 mM

additional information

not inhibited by urea

additional information

arabinose is not inhibitory

additional information

hydroxylamine hydrochloride; not affected by 2-mercaptoethanol; not: EDTA

additional information

not: EDTA

additional information

not affected by 2-mercaptoethanol; not affected by cysteine; not affected by several hexoses, overview

additional information

show all columns Go to Activating Compound Search

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ACTIVATING COMPOUND

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

aspartic acid

5 mM, activates partially purified enzyme

EDTA

1 mM EDTA enhances the enzyme activity by 31%

elugent

slight activation at 1%

ethanol

ethanol increases the fructooligosaccharide biosynthesis activity of levansucrase, fructooligosaccharide production at different temperatures in the presence of ethanol, inverse relationship between temperature and ratio synthetic activity/total activity of levansucrase, overview

fructose

slight activation

488332

fructose 1,6-biphosphate

5 mM, activates partially purified enzyme

glucoseamine-HCl

5 mM, activates partially purified enzyme

levan

4 entries

NaCl

Sodium dodecyl sulfate

slight activation at 5% w/v

sucrose

Klebsiella sp. L1

sucrose concentration at 10% (w/v) is the excellent levansucrase inducer

755924

Tween 20

slight activation at 1%

additional information

levan

accelerates the rate of polymerization of levan, effective only under conditions of low ionic strength

488320

levan

1.4fold increase in Vmax of wild-type in the presence of 15 g/l of levan is observed

levan

3.7fold increase in Vmax of S164A in the presence of 15 g/l of levan is observed

levan

increase of transferase not hydrolase activity

NaCl

maximum activity at 3.0 M

NaCl

the enzyme requires at least 1.5 M NaCl to be active and shows maximum activity in the presence of 3.5 M NaCl

NaCl

Klebsiella sp. L1

maximum activity at 2% (w/v)

755924

additional information

levan has no effect on Y429N, a mutant that has lost the fructan-synthesizing activity

additional information

levan has no effect on Y429N, a mutant that has lost the fructan-synthesizing activity

Go to Disease Search

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DISEASE

TITLE OF PUBLICATION

LINK TO PUBMED

Dental Plaque

Relative effects of sucrolytic enzymes in human dental plaque.

1070129

Infections

Identification of Erwinia amylovora genes induced during infection of immature pear tissue.

16291682

Infections

Purification and characterization of an extracellular levansucrase from Pseudomonas syringae pv. phaseolicola.

7751294

Pancreatic Neoplasms

Optimization strategy of Bacillus subtilis MT453867 levansucrase and evaluation of levan role in pancreatic cancer treatment.

34015407

Periodontal Diseases

Levan and levansucrase of Actinomyces viscosus.

14893

Starvation

Effects of phosphate limitation on expression of genes involved in pyrimidine synthesis and salvaging in Arabidopsis.

15820655

show all columns Go to KM Value Search

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

47.81

D-glucose

pH 6.0, 30°C, recombinant enzyme

2 - 978.37

sucrose

102 entries

additional information

31 entries

sucrose

hydrolytic activity, at pH 6.0 and 37°C

2 - 3

sucrose

total activity, at pH 6.0 and 37°C

2.3

sucrose

pH 6.6, mutant enzyme S173A

2.5

sucrose

mutant enzyme S164A, 0.5 U/ml of enzyme activity at 37°C and pH 6.0

sucrose

total activity, at pH 6.0 and 37°C

sucrose

hydrolytic activity, at pH 6.0 and 37°C

4.1

sucrose

pH 6.6, mutant enzyme N252A

6.6

sucrose

pH 6.6, wild-type enzyme

7.4

sucrose

mutant enzyme I341V, 0.5 U/ml of enzyme activity at 37°C and pH 6.0

sucrose

sucrose

wild-type, 0.5 U/ml of enzyme activity at 37°C and pH 6.0

sucrose

total activity, at pH 6.0 and 37°C

sucrose

transfructosylating activity, at pH 6.0 and 37°C

sucrose

hydrolytic activity, at pH 6.0 and 37°C

sucrose

total activity, at pH 6.0 and 37°C

10.5

sucrose

mutant D309N

10.5

sucrose

mutant enzyme H243L, 0.5 U/ml of enzyme activity at 37°C and pH 6.0

11.4

sucrose

wild-type enzyme

11.9

sucrose

30°C, pH 5.0, wild-type enzyme

sucrose

12.3

sucrose

30°C, pH 5.0, mutant enzyme D135N

13.1

sucrose

30°C, pH 5.0, mutant enzyme C395S

13.5

sucrose

at 37°C

488332

13.8

sucrose

30°C, pH 5.0, mutant enzyme C339S

sucrose

17.1

sucrose

sucrose hydrolysis, pH 6.0-6.6, 60°C

18.5

sucrose

sucrose hydrolysis, pH 6.0-6.6, 60°C

sucrose

19.6

sucrose

wild type enzyme, hydrolytic activity, at pH 6.0 and 50°C

sucrose

hydrolytic activity, at pH 6.0 and 37°C

sucrose

hydrolytic activity, at pH 6.0 and 37°C

22.5

sucrose

24.1

sucrose

sucrose hydrolysis, pH 6.0-6.6, 60°C

26.6

sucrose

sucrose

29.3

sucrose

mutant enzyme R433A, 0.5 U/ml of enzyme activity at 37°C and pH 6.0

sucrose

mutant enzyme R369K, 0.5 U/ml of enzyme activity at 37°C and pH 6.0

sucrose

total activity, at pH 6.0 and 37°C

31.9

sucrose

pH 6.6, mutant enzyme W94A

33.6

sucrose

at pH 6.5 and 37°C

sucrose

Q60114

25°C, pH 5.0, transfructosylation activity

sucrose

sucrose hydrolysis, wild-type

sucrose

Q60114

25°C, pH 5.0, levan synthesis activity is monitored by following the increase in turbidity at 600 nm that occurrs due to the insoluble levan formation

44.46

sucrose

wild type enzyme, total activity, at pH 6.0 and 50°C

44.74

sucrose

mutant enzyme K372Y, hydrolytic activity, at pH 6.0 and 50°C

sucrose

50.5

sucrose

at pH 6.5 and 37°C

51.9

sucrose

pH 6.6, mutant enzyme Y421A

sucrose

at pH 5.9 and 50°C

66.6

sucrose

pH 6.6, mutant enzyme L118A

77.91

sucrose

mutant enzyme K372Y, total activity, at pH 6.0 and 50°C

86.69

sucrose

mutant enzyme K372A, hydrolytic activity, at pH 6.0 and 50°C

110

sucrose

sucrose hydrolysis, mutant R331K

113.13

sucrose

mutant enzyme K372A, transfructosylation activity, at pH 6.0 and 50°C

117.2

sucrose

pH 6.5, 40°C

118

sucrose

mutant enzyme E404K, at pH 6.5 and 45°C

120

sucrose

sucrose hydrolysis, mutant R331H

122

sucrose

125

sucrose

wild-type enzyme

127.96

sucrose

mutant enzyme N251W, transfructosylation activity, at pH 6.0 and 50°C

127.96

sucrose

wild type enzyme, transfructosylation activity, at pH 6.0 and 50°C

131

sucrose

at pH 6.0 and 37°C

137.62

sucrose

mutant enzyme N251W/K372Y, transfructosylation activity, at pH 6.0 and 50°C

154

sucrose

mutant enzyme R360S, 0.5 U/ml of enzyme activity at 37°C and pH 6.0

160

sucrose

160

sucrose

O68609

sucrose hydrolysis, pH 5.8-6.6, 60°C

176.72

sucrose

mutant enzyme K372Y, transfructosylation activity, at pH 6.0 and 50°C

180

sucrose

sucrose hydrolysis, mutants R331L and R331S

193.4

sucrose

mutant enzyme K372A, total activity, at pH 6.0 and 50°C

249

sucrose

mutant enzyme E404P, at pH 6.5 and 45°C

273

sucrose

mutant enzyme E404M, at pH 6.5 and 45°C

283

sucrose

mutant enzyme E404Q, at pH 6.5 and 45°C

284

sucrose

mutant enzyme E404F, at pH 6.5 and 45°C

286

sucrose

mutant enzyme E404H, at pH 6.5 and 45°C

287

sucrose

mutant enzyme E404Y, at pH 6.5 and 45°C

293

sucrose

mutant enzyme E404G, at pH 6.5 and 45°C

294

sucrose

mutant enzyme E404W, at pH 6.5 and 45°C

296

sucrose

mutant enzyme E404L, at pH 6.5 and 45°C

296

sucrose

mutant enzyme E404N, at pH 6.5 and 45°C

296

sucrose

mutant enzyme E404S, at pH 6.5 and 45°C

297.3

sucrose

mutant enzyme G361F, 0.5 U/ml of enzyme activity at 37°C and pH 6.0

305

sucrose

wild type enzyme, at pH 6.5 and 45°C

309

sucrose

mutant enzyme E404V, at pH 6.5 and 45°C

318

sucrose

mutant enzyme E404I, at pH 6.5 and 45°C

319.4

sucrose

mutant enzyme Y429N, 0.5 U/ml of enzyme activity at 37°C and pH 6.0

338

sucrose

mutant enzyme E404T, at pH 6.5 and 45°C

346

sucrose

mutant enzyme E404D, at pH 6.5 and 45°C

355

sucrose

mutant enzyme E404A, at pH 6.5 and 45°C

370

sucrose

mutant enzyme E404C, at pH 6.5 and 45°C

377

sucrose

mutant enzyme E404R, at pH 6.5 and 45°C

436

sucrose

transfructosylating activity, at pH 6.0 and 37°C

440

sucrose

transfructosylating activity, at pH 6.0 and 37°C

467

sucrose

transfructosylating activity, at pH 6.0 and 37°C

479

sucrose

transfructosylating activity, at pH 6.0 and 37°C

480

sucrose

total activity, at pH 6.0 and 37°C

480.4

sucrose

pH 6.6, mutant enzyme W172A

519

sucrose

transfructosylating activity, at pH 6.0 and 37°C

617

sucrose

hydrolytic activity, at pH 6.0 and 37°C

674

sucrose

transfructosylating activity, at pH 6.0 and 37°C

795.22

sucrose

mutant enzyme N251W/K372Y, total activity, at pH 6.0 and 50°C

820.75

sucrose

mutant enzyme N251W, hydrolytic activity, at pH 6.0 and 50°C

978.37

sucrose

mutant enzyme N251W, total activity, at pH 6.0 and 50°C

additional information

additional information

mutants, overview

additional information

Michaelis-Menten kinetics

additional information

Michaelis-Menten kinetics

additional information

Michaelis-Menten kinetics

additional information

kinetics, overview

additional information

2.3 mM (sucrose) total, mutant enzyme S173A

additional information

29.2 mM (sucrose) total, mutant enzyme R370A, a 4fold KM value decrease compared with the wild-type enzyme supported the interactions of Arg 370 with the 2-OH and 3-OH of the glucosyl residue in the active site

additional information

31.9 mM (sucrose) total, mutant enzyme W94A, 9% of the catalytic efficiency of the wild-type

additional information

Q60114

32 mM (sucrose), 25°C, pH 5.0, total activity

additional information

32 mM (sucrose), 25°C, pH 5.0, total activity

additional information

35.1 mM (sucrose) total, mutant enzyme N252H

additional information

4.1 mM (sucrose) total, mutant enzyme N252A

additional information

480.4 mM (sucrose) total, mutant enzyme W172A, 72fold increase of the KM of the wild-type

additional information

51.9 mM (sucrose) total, mutant enzyme Y421A, 3%of the wild-type activity

additional information

6.6 mM (sucrose) total, wild-type, pH 6.6, 37°C, 50 mM Sorensens phosphate buffer, 7.36 mg/l enzyme concentration, reaction time 60 min

additional information

66.6 mM (sucrose), mutant enzyme L118A

additional information

7.5 mM (sucrose) total, mutant enzyme N252G

additional information

8.4 mM (sucrose) total, mutant enzyme N252D

additional information

Q60114

at pHs higher than 7.0, Zymomonas mobilis levansucrase does not exhibit levan synthesis activity

additional information

at pHs higher than 7.0, Zymomonas mobilis levansucrase does not exhibit levan synthesis activity

additional information

mutant enzyme D257A, no activity

additional information

mutant enzyme D95A, no activity

additional information

mutant enzyme E350A, nearly inactive

additional information

mutant enzyme E352A, no measureable activity

additional information

mutant enzyme R256A, nearly inactive

additional information

mutant enzyme S164K is inactive

additional information

mutant enzyme S164K is inactive

additional information

mutants A344P and F414W have the same behavior like the wild-type

additional information

mutants A344P and F414W have the same behavior like the wild-type

additional information

kinetics of free and immobilized enzymes, overview

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TURNOVER NUMBER [1/s]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.0039 - 600000

sucrose

81 entries

additional information

0.0039

sucrose

30°C, pH 5.0, wild-type enzyme

0.0267

sucrose

sucrose hydrolysis, mutant R331S

0.117

sucrose

sucrose hydrolysis, mutant R331L

0.367

sucrose

sucrose hydrolysis, mutant R331H

0.583

sucrose

sucrose hydrolysis, wild-type

sucrose

wild-type enzyme

1.47

sucrose

sucrose hydrolysis, mutant R331K

1.7

sucrose

30°C, pH 5.0, mutant enzyme D135N

6.3

sucrose

mutant enzyme Y429N

6.4

sucrose

mutant enzyme S164A

7.2

sucrose

sucrose hydrolysis, pH 6.0-6.6, 60°C

13.3

sucrose

mutant D309N

13.5

sucrose

mutant enzyme R360S

28.3

sucrose

wild-type enzyme

41.8

sucrose

mutant enzyme K372A, hydrolytic activity, at pH 6.0 and 50°C

57.4

sucrose

mutant enzyme G361F

58.24

sucrose

mutant enzyme N251W/K372Y, transfructosylation activity, at pH 6.0 and 50°C

66.9

sucrose

30°C, pH 5.0, mutant enzyme C339S

71.8

sucrose

30°C, pH 5.0, mutant enzyme C395S

81.77

sucrose

mutant enzyme N251W, transfructosylation activity, at pH 6.0 and 50°C

87.5

sucrose

mutant enzyme R433A

99.69

sucrose

mutant enzyme K372A, transfructosylation activity, at pH 6.0 and 50°C

108

sucrose

levansucrase-endolevanase fusion enzyme, pH and temperature not specified in the publication

141.5

sucrose

mutant enzyme H243L

144.68

sucrose

mutant enzyme K372Y, hydrolytic activity, at pH 6.0 and 50°C

156.86

sucrose

mutant enzyme K372A, total activity, at pH 6.0 and 50°C

164

sucrose

levansucrase, pH and temperature not specified in the publication

164.6

sucrose

wild-type enzyme