EC Number   |
Protein Variants |
Reference |
|---|
    1.4.3.5 | D49A |
decrease in affinity for pyridoxine 5'-phosphate |
391906 |
| 1.4.3.5 | DELTA1-56 |
3.35fold increase in KM-value for pyridoxamine 5'-phosphate compared to wild-type enzyme, 2.95fold increase in KM-value for pyridoxine 5'-phosphate compared to wild-type enzyme, 6fold increase in KI-value for pyridoxal 5'-phosphate compared to wild-type enzyme |
655483 |
| 1.4.3.5 | DELTA238-262 |
inactive mutant enzyme |
655483 |
| 1.4.3.5 | DELTA273-294 |
a deletion mutant lacking the C-terminal 22 amino acids shows that the C-terminus is essential for enzymatic activity |
704628 |
| 1.4.3.5 | DELTAW294 |
a deletion mutant in which only the C-terminal tryptophan is deleted, displays activity similar to the wild type protein for chromophore and pyridoxal formation |
704628 |
| 1.4.3.5 | E50K |
mutant shows activity similar to the wild-type enzyme |
668875 |
| 1.4.3.5 | G118R |
pathogenic variant that is responsible for pyridoxamine 5'-phosphate oxidase deficiency. 51% of the activity as compared to wild-type enzyme. Tm-value of the mutant enzyme (in absence and presence of FMN) is about 10°C lower than the Tm-value of the wilde-type enzyme |
765774 |
| 1.4.3.5 | H199A |
decrease in affinity for pyridoxine 5'-phosphate |
391906 |
| 1.4.3.5 | H199N |
little decrease in pyridoxine 5'-phosphate turnover |
391906 |
| 1.4.3.5 | K145A/F177A |
the Tm-value is about 1°C higher than the Tm-value of the wild-type enzyme |
-, 765076 |
