Literature summary for 1.4.3.5 extracted from

Kang, J.H.; Hong, M.L.; Kim, D.W.; Park, J.; Kang, T.C.; Won, M.H.; Baek, N.I.; Moon, B.J.; Choi, S.Y.; Kwon, O.S.
Genomic organization, tissue distribution and deletion mutation of human pyridoxine 5'-phosphate oxidase (2004), Eur. J. Biochem., 271, 2452-2461.

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
DELTA1-56	3.35fold increase in KM-value for pyridoxamine 5'-phosphate compared to wild-type enzyme, 2.95fold increase in KM-value for pyridoxine 5'-phosphate compared to wild-type enzyme, 6fold increase in KI-value for pyridoxal 5'-phosphate compared to wild-type enzyme	Homo sapiens
DELTA238-262	inactive mutant enzyme	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.0021	-	pyridoxamine 5'-phosphate	pH 8.0, 25°C, wild-type enzyme	Homo sapiens
0.0062	-	pyridoxine 5'-phosphate	pH 8.0, 25°C, wild-type enzyme	Homo sapiens
0.0062	-	pyridoxamine 5'-phosphate	pH 8.0, 25°C, mutant enzyme DELTA1-56	Homo sapiens
0.0208	-	pyridoxine 5'-phosphate	pH 8.0, 25°C, mutant enzyme DELTA1-56	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
A-549 cell	0.7% of the activity in liver	Homo sapiens	-
adrenal gland	11.5% of the activity in liver	Homo sapiens	-
amygdala	13.0% of the activity in liver	Homo sapiens	-
aorta	1.2% of the activity in liver	Homo sapiens	-
atrium	14% of the activity in liver detected in left atrium, 11.6% of the activity in liver detected in tight atrium	Homo sapiens	-
bladder	6.8% of the activity in liver	Homo sapiens	-
bone marrow	11.2% of the activity in liver	Homo sapiens	-
brain	23.8% of the activity in liver	Homo sapiens	-
caudate nucleus	19.0% of the activity in liver	Homo sapiens	-
caudate putamen	2.0% of the activity in liver	Homo sapiens	-
cerebellum	13.3% of the activity in liver detected in left cerebellum, 25.0% of the activity in liver detected in the right cerebellum	Homo sapiens	-
cerebral cortex	25.9% of the activity in liver	Homo sapiens	-
cerebral gyrus	of cerebral cortex, 9.5% of the activity in liver	Homo sapiens	-
colon	1.2% of the activity in liver detected in ascending colon, 7.9% of the activity in liver is detected in transverse colon, 2.8% of the activity in liver is detected in descending colon	Homo sapiens	-
corpus callosum	18.4% of the activity in liver	Homo sapiens	-
DAUDI cell	1.1% of the activity in liver	Homo sapiens	-
duodenum	9.3% of the activity in liver	Homo sapiens	-
esophagus	7.5% of the activity in liver	Homo sapiens	-
frontal lobe	12.4% of the activity in liver	Homo sapiens	-
heart	3.7% of the activity in liver	Homo sapiens	-
HeLa-S3 cell	3.5% of the activity in liver	Homo sapiens	-
hippocampus	12.3% of the activity in liver	Homo sapiens	-
HL-60 cell	2.3% of the activity in liver	Homo sapiens	-
ileocecum	6.1% of the activity in liver	Homo sapiens	-
ileum	5.8% of the activity in liver	Homo sapiens	-
interventricular septum	16.2% of the activity in liver	Homo sapiens	-
jejunum	13.3% of the activity in liver	Homo sapiens	-
K-562 cell	2.5% of the activity in liver	Homo sapiens	-
kidney	85.6% of the activity in liver	Homo sapiens	-
left ventricle	4.1% of the activity in liver detected in the left ventricle	Homo sapiens	-
leukocyte	from peripheral blood, 0.4% of the activity in liver	Homo sapiens	-
liver	30.2% of the activity of adult liver is detected in foetal liver	Homo sapiens	-
lung	4.6% of the activity in liver	Homo sapiens	-
lymph node	4.6% of the activity in liver	Homo sapiens	-
medulla oblongata	5.0% of the activity in liver	Homo sapiens	-
MOLT-4 cell	8.1% of the activity in liver	Homo sapiens	-
nucleus accumbens	5.2% of the activity in liver	Homo sapiens	-
occipital lobe	14.7% of the activity in liver	Homo sapiens	-
ovary	4.3% of the activity in liver	Homo sapiens	-
pancreas	2.5% of the activity in liver	Homo sapiens	-
parietal lobe	13.6% of the activity in liver	Homo sapiens	-
placenta	30.2% of the activity in liver	Homo sapiens	-
pons	6.2% of the activity in liver	Homo sapiens	-
prostate gland	16.5% of the activity in liver	Homo sapiens	-
RAJI cell	6.5% of the activity in liver	Homo sapiens	-
rectum	2.3% of the activity in liver	Homo sapiens	-
right ventricle	20.5% of the activity in liver detected in the right ventricle	Homo sapiens	-
salivary gland	10% of the activity in liver	Homo sapiens	-
skeletal muscle	34.1% of the activity in liver	Homo sapiens	-
spinal cord	1.6% of the activity in liver	Homo sapiens	-
spleen	13.5% of the activity in liver	Homo sapiens	-
stomach	17.2% of the activity in liver	Homo sapiens	-
SW-480 cell	5.1% of the activity in liver	Homo sapiens	-
temporal lobe	12.6% of the activity in liver	Homo sapiens	-
testis	8.4% of the activity in liver	Homo sapiens	-
thalamus	10.8% of the activity in liver	Homo sapiens	-
thymus	6.4% of the activity in liver	Homo sapiens	-
thyroid gland	20.3% of the activity in liver	Homo sapiens	-
trachea	4.8% of the activity in liver	Homo sapiens	-
uterus	2.9% of the activity in liver	Homo sapiens	-
vermiform appendix	0.7% of the activity in liver	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
pyridoxamine 5'-phosphate + H2O + O2	-	Homo sapiens	pyridoxal 5'-phosphate + NH3 + H2O2	-	?
pyridoxine 5'-phosphate + H2O + O2	-	Homo sapiens	pyridoxal 5'-phosphate + H2O2	-	?

Synonyms

Synonyms	Comment	Organism
PNPO	-	Homo sapiens
pyridoxine 5'-phosphate oxidase	-	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
3.8	-	pyridoxal 5'-phosphate	pH 8.5, 25°C, pyridoxine 5'-phosphate as substrate, wild-type enzyme	Homo sapiens
23	-	pyridoxal 5'-phosphate	pH 8.5, 25°C, pyridoxine 5'-phosphate as substrate, mutant enzyme DELTA1-56	Homo sapiens