EC Number |
Protein Variants |
Reference |
---|
2.7.1.176 | K46A/D67T |
site-directed mutagenesis, the change in the Walker A motif enables cloning and expression of genes K46A and D67T in Escherichia coli without coexpression of the antagonistic gene epsilon |
718312 |
2.7.1.176 | more |
a mutant variant truncated after Val-234 lacks the helix K-loop-helix L appendage |
718312 |
2.7.1.176 | more |
construction of truncated variant PezTDELTAC242 , the variant does not accumulate any spontaneous mutations and still retains the toxic phenotype |
718251 |
2.7.1.176 | R157A |
site-directed mutagenesis, the mutation abolishes PezT lethality |
717773 |
2.7.1.176 | R158A |
site-directed mutagenesis, the mutant construct can be cloned into Escherichia coli but not overexpressed |
718312 |
2.7.1.176 | R158A/R171S |
site-directed mutagenesis, the mutant can be cloned and expressed |
718312 |
2.7.1.176 | R170A |
site-directed mutagenesis, the mutation abolishes PezT lethality |
717773 |
2.7.1.176 | R171S |
site-directed mutagenesis |
718312 |
2.7.1.176 | T117V |
site-directed mutagenesis, the mutation abolishes PezT lethality |
717773 |
2.7.1.176 | T120V |
site-directed mutagenesis, the mutation abolishes PezT lethality and a delay in the growth inhibition |
717773 |