Cloned (Comment) | Organism |
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gene pezT, DNA and amino acid sequence determination and analysis, sequence comparison, co-expression of fused His-tagged PezT with His-tagged PezA in Escherichia coli BL21(DE3). The PezA antitoxin represses transcription from PpezAT and the PezT toxin acts as a co-repressor | Streptococcus pneumoniae |
Protein Variants | Comment | Organism |
---|---|---|
D66T | site-directed mutagenesis | Streptococcus pneumoniae |
K45A | site-directed mutagenesis, the mutation abolishes PezT lethality | Streptococcus pneumoniae |
R157A | site-directed mutagenesis, the mutation abolishes PezT lethality | Streptococcus pneumoniae |
R170A | site-directed mutagenesis, the mutation abolishes PezT lethality | Streptococcus pneumoniae |
T117V | site-directed mutagenesis, the mutation abolishes PezT lethality | Streptococcus pneumoniae |
T120V | site-directed mutagenesis, the mutation abolishes PezT lethality and a delay in the growth inhibition | Streptococcus pneumoniae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
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Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pneumoniae | Q97QZ1 | gene pezT | - |
Purification (Comment) | Organism |
---|---|
recombinant fused His-tagged PezT with His-tagged PezA from Escherichia coli BL21(DE3) by nickel affinity chromatography, PezA is cleaved off by thrombin, followed by anion exchange and gel filtration to purifiy PezT and PezA, quantitative real-time RT PCR expression analysis | Streptococcus pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | phosphoryltransfer reaction from ATP/GTP to an unknown substrate is much more likely responsible for toxicity than a simple nucleoside triphosphate hydrolysis | Streptococcus pneumoniae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PezT | - |
Streptococcus pneumoniae |
General Information | Comment | Organism |
---|---|---|
metabolism | expression of PezT and zeta in Escherichia coli lead to temporary inhibition in cell growth and is therefore seen as triggering cell stasis rather than cell death | Streptococcus pneumoniae |
physiological function | PezAT chromosomal toxin-antitoxin system of the human pathogen Streptococcus pneumoniae, the toxicity of PezT is counteracted by PezA, which is encoded immediately upstream of pezT and shares weak sequence similarities in the C-terminal region with the epsilon antitoxin. The pezAT genes form a bicistronic operon that is co-transcribed from a sigma70-like promoter upstream of pezA and is negatively autoregulated with PezA functioning as a transcriptional repressor and PezT as a co-repressor. Inactivation of PezT by PezA, in the inactive PezA2PezT2 heterotetrameric protein complex, the nucleotide binding site of PezT is covered by two helices of PezA | Streptococcus pneumoniae |