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Literature summary for 2.7.1.176 extracted from

  • Khoo, S.K.; Loll, B.; Chan, W.T.; Shoeman, R.L.; Ngoo, L.; Yeo, C.C.; Meinhart, A.
    Molecular and structural characterization of the PezAT chromosomal toxin-antitoxin system of the human pathogen Streptococcus pneumoniae (2007), J. Biol. Chem., 282, 19606-19618.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene pezT, DNA and amino acid sequence determination and analysis, sequence comparison, co-expression of fused His-tagged PezT with His-tagged PezA in Escherichia coli BL21(DE3). The PezA antitoxin represses transcription from PpezAT and the PezT toxin acts as a co-repressor Streptococcus pneumoniae

Protein Variants

Protein Variants Comment Organism
D66T site-directed mutagenesis Streptococcus pneumoniae
K45A site-directed mutagenesis, the mutation abolishes PezT lethality Streptococcus pneumoniae
R157A site-directed mutagenesis, the mutation abolishes PezT lethality Streptococcus pneumoniae
R170A site-directed mutagenesis, the mutation abolishes PezT lethality Streptococcus pneumoniae
T117V site-directed mutagenesis, the mutation abolishes PezT lethality Streptococcus pneumoniae
T120V site-directed mutagenesis, the mutation abolishes PezT lethality and a delay in the growth inhibition Streptococcus pneumoniae

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae Q97QZ1 gene pezT
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Purification (Commentary)

Purification (Comment) Organism
recombinant fused His-tagged PezT with His-tagged PezA from Escherichia coli BL21(DE3) by nickel affinity chromatography, PezA is cleaved off by thrombin, followed by anion exchange and gel filtration to purifiy PezT and PezA, quantitative real-time RT PCR expression analysis Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information phosphoryltransfer reaction from ATP/GTP to an unknown substrate is much more likely responsible for toxicity than a simple nucleoside triphosphate hydrolysis Streptococcus pneumoniae ?
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?

Synonyms

Synonyms Comment Organism
PezT
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Streptococcus pneumoniae

General Information

General Information Comment Organism
metabolism expression of PezT and zeta in Escherichia coli lead to temporary inhibition in cell growth and is therefore seen as triggering cell stasis rather than cell death Streptococcus pneumoniae
physiological function PezAT chromosomal toxin-antitoxin system of the human pathogen Streptococcus pneumoniae, the toxicity of PezT is counteracted by PezA, which is encoded immediately upstream of pezT and shares weak sequence similarities in the C-terminal region with the epsilon antitoxin. The pezAT genes form a bicistronic operon that is co-transcribed from a sigma70-like promoter upstream of pezA and is negatively autoregulated with PezA functioning as a transcriptional repressor and PezT as a co-repressor. Inactivation of PezT by PezA, in the inactive PezA2PezT2 heterotetrameric protein complex, the nucleotide binding site of PezT is covered by two helices of PezA Streptococcus pneumoniae