EC Number   |
Protein Variants |
Reference |
|---|
   2.3.1.184 | E46K |
site-directed mutagenesis, inactive mutant |
666383 |
| 2.3.1.184 | E7K/F147L/P159E/E182G |
strain R2: cells produce twicefold amount of butanoyl homoserine lactone compared to wild type and yield a hexanoyl homoserine lactone level comparable to the butanoyl homoserine lactone concentration |
703201 |
| 2.3.1.184 | E7K/F147L/V201M |
strain R1: cells produce twicefold amount of butanoyl homoserine lactone compared to wild type |
703201 |
| 2.3.1.184 | F28L |
site-directed mutagenesis, the mutant shows 0.18% activity compared to the wild-type enzyme |
666383 |
| 2.3.1.184 | F69L |
site-directed mutagenesis of ExpISCC1, the mutant shows altered substrate acyl-chain lemgth specificity compared to the wild-type enzyme |
665602 |
| 2.3.1.184 | G159E |
site-directed mutagenesis, the mutant shows 44.6% activity compared to the wild-type enzyme |
666383 |
| 2.3.1.184 | G68D |
site-directed mutagenesis, the mutant shows 0.075% activity compared to the wild-type enzyme |
666383 |
| 2.3.1.184 | G68E |
site-directed mutagenesis, inactive mutant |
666383 |
| 2.3.1.184 | K150E/R154QE |
site-directed mutagenesis, the mutant shows highly reduced activity compared to the fully active mutant LasIDELTAG |
666390 |
| 2.3.1.184 | K150Q |
site-directed mutagenesis, the mutant shows similar activity as the fully active mutant LasIDELTAG |
666390 |
