BRENDA - Enzyme Database
show all sequences of 2.3.1.184

Altering the substrate specificity of RhlI by directed evolution

Kambam, P.K.; Eriksen, D.T.; Lajoie, J.; Sayut, D.J.; Sun, L.; ChemBioChem 10, 553-558 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli
Pseudomonas aeruginosa
Engineering
Amino acid exchange
Commentary
Organism
E7K/F147L/P159E/E182G
strain R2: cells produce twicefold amount of butanoyl homoserine lactone compared to wild type and yield a hexanoyl homoserine lactone level comparable to the butanoyl homoserine lactone concentration
Pseudomonas aeruginosa
E7K/F147L/V201M
strain R1: cells produce twicefold amount of butanoyl homoserine lactone compared to wild type
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
butanoyl [acyl carrier protein] + S-adenosyl-L-methionine
Pseudomonas aeruginosa
-
N-butanoyl homoserine lactone + [acyl carrier protein] + 5'-methyl-thioadenosine
-
-
ir
hexanoyl [acyl carrier protein] + S-adenosyl-L-methionine
Pseudomonas aeruginosa
production is only 1/15 of N-butanoyl homoserine lactone
N-hexanoyl homoserine lactone + [acyl carrier protein] + 5'-methyl-thioadenosine
-
-
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas aeruginosa
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
butanoyl [acyl carrier protein] + S-adenosyl-L-methionine
-
703201
Pseudomonas aeruginosa
N-butanoyl homoserine lactone + [acyl carrier protein] + 5'-methyl-thioadenosine
-
-
-
ir
hexanoyl [acyl carrier protein] + S-adenosyl-L-methionine
production is only 1/15 of N-butanoyl homoserine lactone
703201
Pseudomonas aeruginosa
N-hexanoyl homoserine lactone + [acyl carrier protein] + 5'-methyl-thioadenosine
-
-
-
ir
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli
Pseudomonas aeruginosa
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
E7K/F147L/P159E/E182G
strain R2: cells produce twicefold amount of butanoyl homoserine lactone compared to wild type and yield a hexanoyl homoserine lactone level comparable to the butanoyl homoserine lactone concentration
Pseudomonas aeruginosa
E7K/F147L/V201M
strain R1: cells produce twicefold amount of butanoyl homoserine lactone compared to wild type
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
butanoyl [acyl carrier protein] + S-adenosyl-L-methionine
Pseudomonas aeruginosa
-
N-butanoyl homoserine lactone + [acyl carrier protein] + 5'-methyl-thioadenosine
-
-
ir
hexanoyl [acyl carrier protein] + S-adenosyl-L-methionine
Pseudomonas aeruginosa
production is only 1/15 of N-butanoyl homoserine lactone
N-hexanoyl homoserine lactone + [acyl carrier protein] + 5'-methyl-thioadenosine
-
-
ir
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
butanoyl [acyl carrier protein] + S-adenosyl-L-methionine
-
703201
Pseudomonas aeruginosa
N-butanoyl homoserine lactone + [acyl carrier protein] + 5'-methyl-thioadenosine
-
-
-
ir
hexanoyl [acyl carrier protein] + S-adenosyl-L-methionine
production is only 1/15 of N-butanoyl homoserine lactone
703201
Pseudomonas aeruginosa
N-hexanoyl homoserine lactone + [acyl carrier protein] + 5'-methyl-thioadenosine
-
-
-
ir
Other publictions for EC 2.3.1.184
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735510
Britstein
A new N-acyl homoserine lacton ...
uncultured Rhodobacterales bacterium
Appl. Environ. Microbiol.
82
1274-1285
2016
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1
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9
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3
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3
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735547
Mangwani
Involvement of quorum sensing ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa N6P6
Appl. Microbiol. Biotechnol.
99
10283-10297
2015
-
1
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5
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4
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2
-
-
2
-
-
735775
Hansen
Triazole-containing N-acyl hom ...
Escherichia coli, Pseudomonas aeruginosa
Bioorg. Med. Chem.
23
1638-1650
2015
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6
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2
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6
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736148
How
Unravelling the genome of long ...
Acinetobacter sp., Acinetobacter sp. GG2
Front. Microbiol.
6
240
2015
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1
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1
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2
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4
1
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1
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1
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4
1
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735502
Gao
An N-acyl homoserine lactone s ...
Nitrosospira multiformis
Appl. Environ. Microbiol.
80
951-958
2014
-
-
1
1
-
-
-
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-
1
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4
-
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-
-
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-
2
1
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1
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1
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1
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1
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2
1
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-
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1
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-
737246
Chang
Non-antibiotic quorum sensing ...
Pseudomonas aeruginosa
Sci. Rep.
4
7245
2014
-
-
1
-
-
-
3
-
-
-
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4
-
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2
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4
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-
718655
Rampioni
Functional characterization of ...
Pseudomonas aeruginosa, Pseudomonas putida, Pseudomonas putida WCS358
Appl. Environ. Microbiol.
78
726-734
2012
-
-
2
-
-
-
2
-
-
-
-
3
-
4
-
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-
-
-
-
-
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3
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2
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2
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3
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-
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3
-
-
-
-
-
-
-
-
-
2
2
2
2
-
-
720863
Anbazhagan
Detection of quorum sensing si ...
Acinetobacter sp.
PLoS ONE
7
e36696
2012
-
-
1
-
1
-
-
-
-
-
-
3
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
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3
-
-
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-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
719734
Berger
Tropodithietic acid production ...
Phaeobacter inhibens, Phaeobacter inhibens DSM 17395
J. Bacteriol.
193
6576-6585
2011
-
-
-
-
1
-
-
-
-
-
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2
-
8
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2
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1
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2
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-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
720916
Chung
Small-molecule inhibitor bindi ...
Burkholderia glumae, Burkholderia glumae BGR1
Proc. Natl. Acad. Sci. USA
108
12089-12094
2011
-
1
-
-
-
-
2
-
-
-
-
2
-
5
-
-
-
-
-
-
-
-
2
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-
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-
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-
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1
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-
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2
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-
-
2
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-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
720388
Lee
Phenotypic and physiological a ...
Chromobacterium violaceum
Microbiology
156
3762-3772
2010
-
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1
-
-
-
-
-
-
-
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1
-
4
-
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-
-
-
-
-
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1
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-
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-
-
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1
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-
1
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-
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-
-
1
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-
-
-
-
-
-
-
-
-
1
1
-
-
-
720492
Tsai
LuxR-type quorum-sensing regul ...
Agrobacterium tumefaciens, Aliivibrio fischeri, Dickeya chrysanthemi, Escherichia coli, Pantoea stewartii, Pectobacterium carotovorum, Pseudomonas aeruginosa, Pseudomonas syringae, Serratia marcescens, Serratia marcescens SS-1, Serratia sp., Serratia sp. 39006, Yersinia enterocolitica, Yersinia pestis, Yersinia pseudotuberculosis
Mol. Microbiol.
77
1072-1082
2010
-
-
1
-
-
-
-
-
-
-
-
34
-
15
-
-
-
-
-
-
-
-
39
-
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-
-
-
-
-
-
-
-
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-
-
1
-
-
-
-
-
-
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-
-
-
34
-
-
-
-
-
-
-
-
39
-
-
-
-
-
-
-
-
-
2
30
30
2
-
-
701799
Steindler
LasI/R and RhlI/R quorum sensi ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa PUPa3
Appl. Environ. Microbiol.
75
5131-5140
2009
-
-
-
-
1
-
-
-
-
-
-
4
-
7
-
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-
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4
-
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2
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4
-
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-
4
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
701947
Cao
Complex quorum-sensing regulat ...
Mesorhizobium tianshanense
Arch. Microbiol.
191
283-289
2009
-
-
-
-
1
-
-
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-
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-
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1
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1
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-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
702995
Yang
Functional analysis of three A ...
Mesorhizobium loti, Mesorhizobium loti NZP 2213
Can. J. Microbiol.
55
210-214
2009
1
-
1
-
1
-
-
-
-
-
-
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-
9
-
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1
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2
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2
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1
2
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703201
Kambam
Altering the substrate specifi ...
Pseudomonas aeruginosa
ChemBioChem
10
553-558
2009
-
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1
-
2
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-
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-
2
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1
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2
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1
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2
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2
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2
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-
687413
Niu
Isolation and characterization ...
Acinetobacter baumannii
J. Bacteriol.
190
3386-3392
2008
1
1
1
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1
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-
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1
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6
-
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1
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1
1
1
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1
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1
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1
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704275
Hussain
The acyl-homoserine lactone-ty ...
Dickeya chrysanthemi
J. Bacteriol.
190
1045-1053
2008
-
-
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1
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1
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1
1
-
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-
665409
Gould
Specificity of acyl-homoserine ...
Pantoea stewartii
J. Bacteriol.
188
773-783
2006
-
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1
-
5
-
-
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1
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4
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1
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8
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1
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5
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1
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8
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-
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-
665410
Kirwan
Quorum-sensing signal synthesi ...
Yersinia pestis, Yersinia pestis KIM6+
J. Bacteriol.
188
784-788
2006
-
-
1
-
-
-
-
-
-
-
-
10
-
3
-
-
-
-
-
-
-
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12
-
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-
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-
-
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1
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-
-
-
-
-
-
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-
10
-
-
-
-
-
-
-
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12
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
664092
Raychaudhuri
Chemical mechanism and substra ...
Pseudomonas aeruginosa
Biochemistry
44
2974-2981
2005
-
-
1
-
-
-
-
4
-
1
-
1
-
2
-
-
1
1
-
-
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5
-
1
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4
1
1
-
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-
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1
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-
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4
-
1
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1
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1
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-
5
-
1
-
-
4
1
1
-
-
-
-
-
-
-
-
665602
Brader
Altering substrate chain lengt ...
Pectobacterium carotovorum subsp. carotovorum
J. Biol. Chem.
280
10403-10409
2005
-
-
1
-
2
-
-
-
-
-
-
2
-
5
-
-
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1
-
-
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5
-
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1
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2
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2
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5
-
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-
663701
Ulrich
Quorum quenching: Enzymatic di ...
Burkholderia thailandensis
Appl. Environ. Microbiol.
70
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