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Literature summary for 2.3.1.184 extracted from

  • Parsek, M.R.; Schaefer, A.L.; Greenberg, E.P.
    Analysis of random and site-directed mutations in rhlI, a Pseudomonas aeruginosa gene encoding an acylhomoserine lactone synthase (1997), Mol. Microbiol., 26, 301-310.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in Escherichia coli Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
C126S site-directed mutagenesis, the mutant shows 81.5% activity compared to the wild-type enzyme Pseudomonas aeruginosa
C67S site-directed mutagenesis, the mutant shows 1.9% activity compared to the wild-type enzyme Pseudomonas aeruginosa
C67S/C69S site-directed mutagenesis, the mutant shows 0.8% activity compared to the wild-type enzyme Pseudomonas aeruginosa
C69S site-directed mutagenesis, the mutant shows 30.4% activity compared to the wild-type enzyme Pseudomonas aeruginosa
C89S site-directed mutagenesis, the mutant shows 63% activity compared to the wild-type enzyme Pseudomonas aeruginosa
D48N site-directed mutagenesis, inactive mutant Pseudomonas aeruginosa
D51N site-directed mutagenesis, nearly inactive mutant Pseudomonas aeruginosa
E101K site-directed mutagenesis, inactive mutant Pseudomonas aeruginosa
E144K site-directed mutagenesis, the mutant shows 43.5% activity compared to the wild-type enzyme Pseudomonas aeruginosa
E46K site-directed mutagenesis, inactive mutant Pseudomonas aeruginosa
F28L site-directed mutagenesis, the mutant shows 0.18% activity compared to the wild-type enzyme Pseudomonas aeruginosa
G159E site-directed mutagenesis, the mutant shows 44.6% activity compared to the wild-type enzyme Pseudomonas aeruginosa
G68D site-directed mutagenesis, the mutant shows 0.075% activity compared to the wild-type enzyme Pseudomonas aeruginosa
G68E site-directed mutagenesis, inactive mutant Pseudomonas aeruginosa
additional information screening of diverse mutants constructrd by random mutagenesis, overview Pseudomonas aeruginosa
R104C site-directed mutagenesis, nearly inactive mutant Pseudomonas aeruginosa
R104H site-directed mutagenesis, nearly inactive mutant Pseudomonas aeruginosa
R24W site-directed mutagenesis, inactive mutant Pseudomonas aeruginosa
R71C site-directed mutagenesis, the mutant shows 0.05% activity compared to the wild-type enzyme Pseudomonas aeruginosa
R71H site-directed mutagenesis, inactive mutant Pseudomonas aeruginosa
S103E site-directed mutagenesis, the mutant shows 5.4% activity compared to the wild-type enzyme Pseudomonas aeruginosa
W34G site-directed mutagenesis, the mutant shows 0.10% activity compared to the wild-type enzyme Pseudomonas aeruginosa
W34Y site-directed mutagenesis, the mutant shows 60% activity compared to the wild-type enzyme Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pseudomonas aeruginosa the enzyme is involved in quorum-sensing signaling by synthesizing the signaling molecules, the autoinducers N-acylhomoserine lactones, the enzyme acts as a quorum-sensing signal generator ?
-
?
S-adenosyl-L-methionine + butyryl-[acyl-carrier protein] Pseudomonas aeruginosa
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5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa P54291 RhlI; gene rhlI
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Reaction

Reaction Comment Organism Reaction ID
an acyl-[acyl-carrier protein] + S-adenosyl-L-methionine = an [acyl-carrier protein] + S-methyl-5'-thioadenosine + an N-acyl-L-homoserine lactone RhlI contains no active site cysteine, residues R24, F28, W34, E46, D48, D51, G68, C67, R71, E101, S103, and R104 are critical for catalytic activity Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is involved in quorum-sensing signaling by synthesizing the signaling molecules, the autoinducers N-acylhomoserine lactones, the enzyme acts as a quorum-sensing signal generator Pseudomonas aeruginosa ?
-
?
S-adenosyl-L-methionine + butyryl-[acyl-carrier protein]
-
Pseudomonas aeruginosa 5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
?

Synonyms

Synonyms Comment Organism
acylhomoserine lactone synthase
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Pseudomonas aeruginosa
autoinducer synthesis protein rhlI
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Pseudomonas aeruginosa
RhlI
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Pseudomonas aeruginosa