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EC Number	Protein Variants	Commentary	Reference
2.1.1.224	F118A	mutation eliminates the resistance to both florfenicol and tiamulin	705983
2.1.1.224	H214A	moderately decreased resistance to both florfenicol and tiamulin	705983
2.1.1.224	Q28A	mutation eliminates the resistance to both florfenicol and tiamulin	705983
2.1.1.224	R25A	mutation lowers the resistance to both florfenicol and tiamulin considerably	705983
2.1.1.224	S189A	mutation lowers the resistance to both florfenicol and tiamulin considerably	705983
2.1.1.224	S212A	mutation eliminates the resistance to both florfenicol and tiamulin	705983
2.1.1.224	C113A	no significant reduction in activity	706726
2.1.1.224	C115A	mutation of the cysteines in the presumed radical S-adenosyl-L-methionine motif CxxxCxxC abolishes Cfr activity	706726
2.1.1.224	C119A	mutation of the cysteines in the presumed radical S-adenosyl-L-methionine motif CxxxCxxC abolishes Cfr activity	706726
2.1.1.224	C122A	mutation of the cysteines in the presumed radical S-adenosyl-L-methionine motif CxxxCxxC abolishes Cfr activity	706726

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Release 2023.1 (January 2023)