BRENDA - Enzyme Database show
show all sequences of 2.1.1.224

Identification of 8-methyladenosine as the modification catalyzed by the radical SAM methyltransferase Cfr that confers antibiotic resistance in bacteria

Giessing, A.M.; Jensen, S.S.; Rasmussen, A.; Hansen, L.H.; Gondela, A.; Long, K.; Vester, B.; Kirpekar, F.; RNA 15, 327-336 (2009)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
C113A
no significant reduction in activity
Escherichia coli
C115A
mutation of the cysteines in the presumed radical S-adenosyl-L-methionine motif CxxxCxxC abolishes Cfr activity
Escherichia coli
C119A
mutation of the cysteines in the presumed radical S-adenosyl-L-methionine motif CxxxCxxC abolishes Cfr activity
Escherichia coli
C122A
mutation of the cysteines in the presumed radical S-adenosyl-L-methionine motif CxxxCxxC abolishes Cfr activity
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Escherichia coli
Cfr confers a phenotype with resistance to phenicols, lincosamides, oxazolidinones, pleuromutilins, and streptogramin A antibiotics. Methylation of position 8 is the dominant antibiotic resistance determinant, but indigenous modification at position 2 also contributes to low-level resistance
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
C6EKJ2
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
706726
Escherichia coli
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
-
-
?
additional information
Cfr confers a phenotype with resistance to phenicols, lincosamides, oxazolidinones, pleuromutilins, and streptogramin A antibiotics. Methylation of position 8 is the dominant antibiotic resistance determinant, but indigenous modification at position 2 also contributes to low-level resistance
706726
Escherichia coli
?
-
-
-
-
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
C113A
no significant reduction in activity
Escherichia coli
C115A
mutation of the cysteines in the presumed radical S-adenosyl-L-methionine motif CxxxCxxC abolishes Cfr activity
Escherichia coli
C119A
mutation of the cysteines in the presumed radical S-adenosyl-L-methionine motif CxxxCxxC abolishes Cfr activity
Escherichia coli
C122A
mutation of the cysteines in the presumed radical S-adenosyl-L-methionine motif CxxxCxxC abolishes Cfr activity
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Escherichia coli
Cfr confers a phenotype with resistance to phenicols, lincosamides, oxazolidinones, pleuromutilins, and streptogramin A antibiotics. Methylation of position 8 is the dominant antibiotic resistance determinant, but indigenous modification at position 2 also contributes to low-level resistance
?
-
-
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
706726
Escherichia coli
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
-
-
?
additional information
Cfr confers a phenotype with resistance to phenicols, lincosamides, oxazolidinones, pleuromutilins, and streptogramin A antibiotics. Methylation of position 8 is the dominant antibiotic resistance determinant, but indigenous modification at position 2 also contributes to low-level resistance
706726
Escherichia coli
?
-
-
-
-
General Information
General Information
Commentary
Organism
physiological function
Cfr confers a phenotype with resistance to phenicols, lincosamides, oxazolidinones, pleuromutilins, and streptogramin A antibiotics. The RlmN knock out strain JW2501-1 is less sensitive to the antibiotics than standard laboratory strain HB101
Escherichia coli
General Information (protein specific)
General Information
Commentary
Organism
physiological function
Cfr confers a phenotype with resistance to phenicols, lincosamides, oxazolidinones, pleuromutilins, and streptogramin A antibiotics. The RlmN knock out strain JW2501-1 is less sensitive to the antibiotics than standard laboratory strain HB101
Escherichia coli
Other publictions for EC 2.1.1.224
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735450
Hansen
A cfr-like gene from Clostridi ...
Clostridioides difficile
Antimicrob. Agents Chemother.
59
5841-5843
2015
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1
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1
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4
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1
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1
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1
1
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733111
Atkinson
Distinction between the Cfr me ...
Staphylococcus sciuri
Antimicrob. Agents Chemother.
57
4019-4026
2013
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1
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1
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1
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1
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1
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1
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1
1
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1
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1
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2
2
-
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735095
Challand
Cysteine methylation controls ...
Staphylococcus aureus, Staphylococcus sciuri
PLoS ONE
8
e67979
2013
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2
-
2
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2
3
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2
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1
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1
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3
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2
3
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2
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2
3
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4
1
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-
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2
2
-
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-
736280
Wang
-
Quantum chemistry studies of a ...
Staphylococcus aureus
Int. J. Quantum Chem.
113
1409-1415
2013
-
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1
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2
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1
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1
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2
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1
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3
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718598
Locke
Genetic environment and stabil ...
Staphylococcus aureus, Staphylococcus aureus CM05
Antimicrob. Agents Chemother.
56
332-340
2012
-
-
1
-
1
-
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4
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1
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718601
Hansen
The order Bacillales hosts fun ...
Bacillus amyloliquefaciens, Bacillus amyloliquefaciens FZB42, Bacillus clausii, Brevibacillus brevis, Brevibacillus brevis NBRC 100599
Antimicrob. Agents Chemother.
56
3563-3567
2012
-
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3
-
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5
-
9
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10
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3
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3
3
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5
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10
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6
6
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716783
Yan
RNA methylation by radical SAM ...
Escherichia coli
Proc. Natl. Acad. Sci. USA
108
3930-3934
2011
-
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1
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1
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1
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716926
Boal
Structural basis for methyl tr ...
Escherichia coli
Science
332
1089-1092
2011
-
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1
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716927
Grove
A radically different mechanis ...
Escherichia coli
Science
332
604-607
2011
-
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718587
LaMarre
Low fitness cost of the multid ...
Staphylococcus aureus, Staphylococcus aureus RN4220
Antimicrob. Agents Chemother.
55
3714-3719
2011
-
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1
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1
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1
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2
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19
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1
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2
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1
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1
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1
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1
1
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1
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1
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2
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1
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2
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1
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1
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2
2
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704203
Yan
RlmN and Cfr are radical SAM e ...
Escherichia coli
J. Am. Chem. Soc.
132
3953-3964
2010
-
-
1
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3
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1
1
1
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1
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1
1
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3
1
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1
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3
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1
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720968
Booth
High-level expression and reco ...
Azotobacter vinelandii
Protein Expr. Purif.
74
204-210
2010
1
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1
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-
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-
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1
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1
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3
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1
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2
1
1
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1
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1
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1
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1
1
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1
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1
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1
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2
1
1
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1
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1
1
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705983
Kaminska
Insights into the structure, f ...
Escherichia coli
Nucleic Acids Res.
38
1652-1663
2009
-
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1
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13
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1
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2
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1
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13
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1
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1
1
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706726
Giessing
Identification of 8-methyladen ...
Escherichia coli
RNA
15
327-336
2009
-
-
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4
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1
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3
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2
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1
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2
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1
1
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701724
Long
The Cfr rRNA methyltransferase ...
Escherichia coli
Antimicrob. Agents Chemother.
50
2500-2505
2006
-
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6
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1
1
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705766
Kehrenberg
A new mechanism for chloramphe ...
Escherichia coli
Mol. Microbiol.
57
1064-1073
2005
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