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Literature summary for 2.1.1.224 extracted from

  • Giessing, A.M.; Jensen, S.S.; Rasmussen, A.; Hansen, L.H.; Gondela, A.; Long, K.; Vester, B.; Kirpekar, F.
    Identification of 8-methyladenosine as the modification catalyzed by the radical SAM methyltransferase Cfr that confers antibiotic resistance in bacteria (2009), RNA, 15, 327-336.
    View publication on PubMedView publication on EuropePMC
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Protein Variants

Protein Variants Comment Organism
C113A no significant reduction in activity Escherichia coli K-12
C115A mutation of the cysteines in the presumed radical S-adenosyl-L-methionine motif CxxxCxxC abolishes Cfr activity Escherichia coli K-12
C119A mutation of the cysteines in the presumed radical S-adenosyl-L-methionine motif CxxxCxxC abolishes Cfr activity Escherichia coli K-12
C122A mutation of the cysteines in the presumed radical S-adenosyl-L-methionine motif CxxxCxxC abolishes Cfr activity Escherichia coli K-12

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli K-12 Cfr confers a phenotype with resistance to phenicols, lincosamides, oxazolidinones, pleuromutilins, and streptogramin A antibiotics. Methylation of position 8 is the dominant antibiotic resistance determinant, but indigenous modification at position 2 also contributes to low-level resistance ?
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 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli K-12 P36979
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
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 Escherichia coli K-12 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
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 ?
additional information Cfr confers a phenotype with resistance to phenicols, lincosamides, oxazolidinones, pleuromutilins, and streptogramin A antibiotics. Methylation of position 8 is the dominant antibiotic resistance determinant, but indigenous modification at position 2 also contributes to low-level resistance Escherichia coli K-12 ?
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 ?

Synonyms

Synonyms Comment Organism
Cfr
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 Escherichia coli K-12

General Information

General Information Comment Organism
physiological function Cfr confers a phenotype with resistance to phenicols, lincosamides, oxazolidinones, pleuromutilins, and streptogramin A antibiotics. The RlmN knock out strain JW2501-1 is less sensitive to the antibiotics than standard laboratory strain HB101 Escherichia coli K-12