EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
4.1.99.2 | -999 |
- |
more |
pH-independent kinetic constants for substrates bearing small substituents |
33467 |
4.1.99.2 | -999 |
- |
more |
the turnover-numbers of the mutant enzyme R381I, R381V and R381A for the substrates S-(o-nitrophenyl)-L-Cys, 3-chloro-L-Ala, O-benzoyl-L-Ser and S-methyl-L-Cys are comparable to those of the wild-type enzyme |
33470 |
4.1.99.2 | 0.000034 |
- |
L-tyrosine |
pH 8.0, 25°C, recombinant mutant F448H |
746580 |
4.1.99.2 | 0.00013 |
- |
L-tyrosine |
pH 8.0, 25°C, mutant enzyme K256H |
664018 |
4.1.99.2 | 0.00016 |
- |
L-tyrosine |
pH 8.0, 25°C, recombinant mutant F449A |
746580 |
4.1.99.2 | 0.000217 |
- |
S-ethyl-L-Cys |
mutant enzyme Y71F |
33471 |
4.1.99.2 | 0.00026 |
- |
L-tyrosine |
pH 8.0, 25°C, recombinant mutant F448A |
746580 |
4.1.99.2 | 0.000383 |
- |
S-methyl-L-Cys |
mutant enzyme Y71F |
33471 |
4.1.99.2 | 0.0008 |
- |
Phe |
beta-elimination |
33443 |
4.1.99.2 | 0.001 |
- |
L-tyrosine |
pH 8.0, 25°C, mutant enzyme K256A |
664018 |