Protein Variants | Comment | Organism |
---|---|---|
E69D/K256R | no activity with L-Tyr, turnover-number for S-(o-nitrophenyl)-L-cysteine is 1.5fold lower than the wild-type value, turnover-number for S-ethyl-L-Cys is 11fold lower than the wild-type value | Citrobacter freundii |
K256A | turnover-number for L-Tyr is 3500fold lower than wild-type value, turnover-number for S-(o-nitrophenyl)-L-cysteine is 560fold lower than the wild-type value, turnover-number for S-ethyl-L-Cys is 1560fold lower than the wild-type value, activity is not increased by addition of monovalent cations, K+, Na+, Li+, Rb+, or NH4+ | Citrobacter freundii |
K256H | turnover-number for L-Tyr is 26923fold lower than wild-type value, turnover-number for S-(o-nitrophenyl)-L-cysteine is 189fold lower than the wild-type value, turnover-number for S-ethyl-L-Cys is 443fold lower than the wild-type value, activity is not increased by addition of monovalent cations, K+, Na+, Li+, Rb+, or NH4+ | Citrobacter freundii |
K256R | turnover-number for L-Tyr is 29fold lower than wild-type value, turnover-number for S-(o-nitrophenyl)-L-cysteine is 30fold lower than the wild-type value, turnover-number for S-ethyl-L-Cys is 195fold lower than the wild-type value | Citrobacter freundii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrobacter freundii | P31013 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tyrosine + H2O | - |
Citrobacter freundii | phenol + pyruvate + NH3 | - |
? | |
S-(o-nitrophenyl)-L-cysteine + H2O | - |
Citrobacter freundii | ? | - |
? | |
S-ethyl-L-Cys + H2O | - |
Citrobacter freundii | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00013 | - |
L-tyrosine | pH 8.0, 25°C, mutant enzyme K256H | Citrobacter freundii | |
0.001 | - |
L-tyrosine | pH 8.0, 25°C, mutant enzyme K256A | Citrobacter freundii | |
0.0025 | - |
S-ethyl-L-Cys | pH 8.0, 25°C, mutant enzyme K256A | Citrobacter freundii | |
0.0088 | - |
S-ethyl-L-Cys | pH 8.0, 25°C, mutant enzyme K256H | Citrobacter freundii | |
0.0091 | - |
S-(o-nitrophenyl)-L-cysteine | pH 8.0, 25°C, mutant enzyme K256A | Citrobacter freundii | |
0.02 | - |
S-ethyl-L-Cys | pH 8.0, 25°C, mutant enzyme K256R | Citrobacter freundii | |
0.027 | - |
S-(o-nitrophenyl)-L-cysteine | pH 8.0, 25°C, mutant enzyme K256H | Citrobacter freundii | |
0.12 | - |
L-tyrosine | pH 8.0, 25°C, mutant enzyme K256R | Citrobacter freundii | |
0.17 | - |
S-(o-nitrophenyl)-L-cysteine | pH 8.0, 25°C, mutant enzyme K256R | Citrobacter freundii | |
0.37 | - |
S-ethyl-L-Cys | pH 8.0, 25°C, mutant enzyme E69D/K256R | Citrobacter freundii | |
7.4 | - |
S-(o-nitrophenyl)-L-cysteine | pH 8.0, 25°C, mutant enzyme E69D/K256R | Citrobacter freundii |