EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
|---|
   3.2.1.45 | 0.007 |
- |
C18-glucosylceramide |
in 50 mM MES buffer, pH 6.0, at 37°C |
680835 |
| 3.2.1.45 | 0.012 |
- |
2,4-dinitrophenyl beta-D-glucopyranoside |
pH 6.5, 37°C, mutant D223G |
654535 |
| 3.2.1.45 | 0.012 |
- |
p-nitrophenyl beta-D-glucopyranoside |
pH 6.5, 37°C, mutant D223G |
654535 |
| 3.2.1.45 | 0.03 |
- |
(6-[N-(7-nitrobenz-2-oxa-1,3 diazol-4-yl)amino]hexanoyl)-beta-D-galactosylceramide |
in 50 mM MES buffer, pH 6.0, at 37°C |
680835 |
| 3.2.1.45 | 0.12 |
- |
(6-[N-(7-nitrobenz-2-oxa-1,3 diazol-4-yl)amino]hexanoyl)-beta-D-glucosylceramide |
in 50 mM MES buffer, pH 6.0, at 37°C |
680835 |
| 3.2.1.45 | 1.23 |
- |
4-methylumbelliferyl-beta-D-glucopyranoside |
in 50 mM MES buffer, pH 6.0, at 37°C |
680835 |
| 3.2.1.45 | 1.57 |
- |
4-methylumbelliferyl-beta-D-galactopyranoside |
in 50 mM MES buffer, pH 6.0, at 37°C |
680835 |
| 3.2.1.45 | 3.47 |
- |
4-nitrophenyl beta-D-glucoside |
mutant enzyme D508H, at pH 5.5 and 60°C |
749456 |
| 3.2.1.45 | 30.2 |
- |
4-nitrophenyl beta-D-glucoside |
mutant enzyme D508N, at pH 5.5 and 60°C |
749456 |
| 3.2.1.45 | 31.67 |
- |
glucosylceramide |
imiglucerase alfa, in 50 mM sodium citrate, pH 6.0 with 0.75 mg/ml bovine serum albumin, at 37°C |
703910 |
