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Literature summary for 3.2.1.45 extracted from

  • Paal, K.; Ito, M.; Withers, S.G.
    Paenibacillus sp. TS12 glucosylceramidase: kinetic studies of a novel sub-family of family 3 glycosidases and identification of the catalytic residues (2004), Biochem. J., 378, 141-149.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.45

Protein Variants

Protein Variants Comment Organism
D223G the mutation reduces the activity, but can be partially restored by addition of formate Paenibacillus sp.
E411A the mutation reduces the activity, but can be partially restored by addition of azide Paenibacillus sp.

Inhibitors

Inhibitors Comment Organism Structure
2-deoxy-2-fluoro-beta-D-glucopyranoside
-
 Paenibacillus sp.
p-nitrophenyl beta-D-glucopyranoside inhibits mutant D223G Paenibacillus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0003
-
 2,4-dinitrophenyl beta-D-glucopyranoside pH 6.5, 37°C, mutant E411A Paenibacillus sp.
0.018
-
 p-nitrophenyl beta-D-glucopyranoside pH 6.5, 37°C, mutant E411A Paenibacillus sp.
0.15
-
 2,4-dinitrophenyl beta-D-glucopyranoside pH 6.5, 37°C, mutant D223G Paenibacillus sp.
0.16
-
 2,4-dinitrophenyl beta-D-glucopyranoside pH 6.5, 37°C, wild-type enzyme Paenibacillus sp.
0.27
-
 p-nitrophenyl beta-D-glucopyranoside pH 6.5, 37°C, wild-type enzyme Paenibacillus sp.
0.33
-
 p-nitrophenyl beta-D-glucopyranoside pH 6.5, 37°C, mutant D223G Paenibacillus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
92020
-
 electrospray ionisation mass spectrometry Paenibacillus sp.

Organism

Organism UniProt Comment Textmining
Paenibacillus sp.
-
 TS12
-

Reaction

Reaction Comment Organism Reaction ID
a D-glucosyl-N-acylsphingosine + H2O = D-glucose + a ceramide the enzyme hydrolyses the beta-glucosidic linkage of glucosylceramide with retention of anomeric conformation via a two-step, double-displacement mechanism Paenibacillus sp.
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-dinitrophenyl beta-D-glucopyranoside + H2O
-
 Paenibacillus sp. p-nitrophenol + beta-D-glucose
-
 ?
p-nitrophenyl beta-D-glucopyranoside + H2O
-
 Paenibacillus sp. p-nitrophenol + beta-D-glucose
-
 ?

Synonyms

Synonyms Comment Organism
Gcase
-
 Paenibacillus sp.
glycosylceramidase
-
 Paenibacillus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.012
-
 p-nitrophenyl beta-D-glucopyranoside pH 6.5, 37°C, mutant D223G Paenibacillus sp.
0.012
-
 2,4-dinitrophenyl beta-D-glucopyranoside pH 6.5, 37°C, mutant D223G Paenibacillus sp.
470
-
 2,4-dinitrophenyl beta-D-glucopyranoside pH 6.5, 37°C, wild-type enzyme Paenibacillus sp.
560
-
 p-nitrophenyl beta-D-glucopyranoside pH 6.5, 37°C, wild-type enzyme Paenibacillus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5
-
 rapid loss Paenibacillus sp.
6 8 stable Paenibacillus sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.7
-
 p-nitrophenyl beta-D-glucopyranoside pH 6.5, 37°C, mutant D223G Paenibacillus sp.
2.4
-
 2-deoxy-2-fluoro-beta-D-glucopyranoside pH 6.5, 37°C Paenibacillus sp.