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EC Number Substrates Commentary Substrates Organism Products Commentary (Products) Reversibility
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.2242 S-adenosyl-L-methionine + adenine2503 in 23S rRNA - Mammaliicoccus sciuri 2 S-adenosyl-L-homocysteine + 2,8-dimethyladenine2503 in 23S rRNA - ?
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.224more Cfr confers a phenotype with resistance to phenicols, lincosamides, oxazolidinones, pleuromutilins, and streptogramin A antibiotics. Methylation of position 8 is the dominant antibiotic resistance determinant, but indigenous modification at position 2 also contributes to low-level resistance Escherichia coli K-12 ? - ?
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.224more enzyme Cfr methylates adenosine 2503 of the 23S rRNA in the peptidyltransferase centre (P-site) of the bacterial ribosome. In wild-type Cfr, where Cys338 is methylated, S-adenosyl-L-methionine binding leads to rapid oxidation of the [4Fe-4S] cluster and production of 5'-deoxyadenosine Staphylococcus aureus ? - ?
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.224more methylation at C8 is similar to that at C2, cf. EC 2.1.1.192 Staphylococcus aureus ? - ?
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.224more Cfr methylates A2503 of 23S rRNA at C8 and C2, while RlmN only performs a C2 methylation using the same mechanism of function Mammaliicoccus sciuri ? - -
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.224more in vitro assays with Escherichia coli RNA Clostridioides difficile ? - -
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.224more kinetic analysis of methylation of a 155mer rRNA substrate by Cfr enzymes under limiting and under excess substrate conditions initiated with a protein-based reductant, substrate sequence, overview Bacillus amyloliquefaciens ? - -
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.224more kinetic analysis of methylation of a 155mer rRNA substrate by Cfr enzymes under limiting and under excess substrate conditions initiated with a protein-based reductant, substrate sequence, overview Enterococcus faecalis ? - -
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.224more kinetic analysis of methylation of a 155mer rRNA substrate by Cfr enzymes under limiting and under excess substrate conditions initiated with a protein-based reductant, substrate sequence, overview Paenibacillus lautus ? - -
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.224more kinetic analysis of methylation of a 155mer rRNA substrate by Cfr enzymes under limiting and under excess substrate conditions initiated with a protein-based reductant, substrate sequence, overview Clostridioides difficile ? - -
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