EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
2.1.1.224 | 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA |
- |
Alkalihalobacillus clausii |
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA |
- |
? |
2.1.1.224 | 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA |
- |
Alkalihalobacillus clausii |
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA |
verification of RNA methylation at A2503 in 23S rRNA by primer extension method |
? |
2.1.1.224 | 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA |
the enzyme methylates the 8 position of 23S rRNA residue A2503 to confer resistance to multiple antibiotic classes acting upon the large subunit of the bacterial ribosome |
Azotobacter vinelandii |
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA |
- |
? |
2.1.1.224 | 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA |
in the presence of sodium dithionite reconstituted Cfr is both reducible and able to cleave S-adenosyl-L-methionine to 5'-deoxyadeonsine, DOA |
Azotobacter vinelandii |
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA |
- |
? |
2.1.1.224 | 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA |
- |
Bacillus amyloliquefaciens |
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA |
- |
? |
2.1.1.224 | 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA |
- |
Bacillus amyloliquefaciens |
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA |
verification of RNA methylation at A2503 in 23S rRNA by primer extension method |
? |
2.1.1.224 | 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin |
- |
Bacillus amyloliquefaciens |
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin |
- |
? |
2.1.1.224 | more |
kinetic analysis of methylation of a 155mer rRNA substrate by Cfr enzymes under limiting and under excess substrate conditions initiated with a protein-based reductant, substrate sequence, overview |
Bacillus amyloliquefaciens |
? |
- |
- |
2.1.1.224 | 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin |
- |
Bacillus amyloliquefaciens DSM-7 |
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin |
- |
? |
2.1.1.224 | more |
kinetic analysis of methylation of a 155mer rRNA substrate by Cfr enzymes under limiting and under excess substrate conditions initiated with a protein-based reductant, substrate sequence, overview |
Bacillus amyloliquefaciens DSM-7 |
? |
- |
- |