EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
  1.1.9.1 | (S)-2-butanol + ferricyanide |
enzyme electrode, 14% of vmax with 1-butanol, soluble enzyme, 13% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 13fold preferred over (R)-enantiomer, with soluble enzyme 13.5fold, respectively |
Comamonas testosteroni |
butan-2-one + ferrocyanide |
- |
? |
| 1.1.9.1 | (S)-2-heptanol + ferricyanide |
enzyme electrode, 70% of vmax with 1-butanol, soluble enzyme, 63% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 82fold preferred over (R)-enantiomer, with soluble enzyme 315fold, respectively |
Comamonas testosteroni |
heptan-2-one + ferrocyanide |
- |
? |
| 1.1.9.1 | (S)-2-hexanol + ferrocyanide |
enzyme electrode, 74% of vmax with 1-butanol, soluble enzyme, 71% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 35fold preferred over (R)-enantiomer, with soluble enzyme 105fold, respectively |
Comamonas testosteroni |
hexan-2-one + ferricyanide |
- |
? |
| 1.1.9.1 | (S)-2-octanol + ferrocyanide |
enzyme electrode, 82% of vmax with 1-butanol, soluble enzyme, 80% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 82fold preferred over (R)-enantiomer, with soluble enzyme 800fold, respectively |
Comamonas testosteroni |
octan-2-one + ferricyanide |
- |
? |
| 1.1.9.1 | (S)-glycidol + ferrocyanide |
enzyme electrode, 30% of vmax with 1-butanol, soluble enzyme, 1.6% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 0.9fold preferred over (R)-enantiomer, with soluble enzyme no disticnction |
Comamonas testosteroni |
? + ferricyanide |
- |
? |
| 1.1.9.1 | (S)-solketal + 2 ferricyanide |
the second step in (S)-solketal conversion is much faster than the first one and that opposite enantiomeric preferences exist for the alcohol and the aldehyde substrates. (R)-enantiomer is prefered, enantiomeric ratio is 30 for initial rate measurement, 117 for progress curve analysis |
Comamonas testosteroni |
(S)-solketal aldehyde + 2 ferrocyanide + 2 H+ |
- |
r |
| 1.1.9.1 | (S)-solketal + ferrocyanide |
enzyme electrode, 9% of vmax with 1-butanol, 0.7% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 39fold preferred over (R)-enantiomer, with soluble enzyme 117fold, respectively |
Comamonas testosteroni |
? + ferricyanide |
- |
? |
| 1.1.9.1 | 1,2-butanediol + ferricyanide |
ADH IIB, 84% of the activity with 1-butanol. ADH IIG, 86% of the activity with 1,2-propanediol |
Pseudomonas putida |
2-hydroxybutanal + ferrocyanide |
- |
? |
| 1.1.9.1 | 1,2-cyclohexanediol + ferricyanide |
- |
Comamonas testosteroni |
2-hydroxycyclohexanone + ferrocyanide |
- |
? |
| 1.1.9.1 | 1,2-ethanediol + ferricyanide |
ADH IIB, 0% of the activity with 1-butanol. ADH IIG, 71% of the activity with 1,2-propanediol |
Pseudomonas putida |
hydroxyacetaldehyde + ferrocyanide |
- |
? |
