1.1.9.1 (R)-2-butanol + ferricyanide enzyme electrode, 6.5% of vmax with 1-butanol, soluble enzyme, 6% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 13fold preferred over (R)-enantiomer, with soluble enzyme 13.5fold, respectively Comamonas testosteroni butan-2-one + ferrocyanide - ? 407635 1.1.9.1 (R)-2-heptanol + ferricyanide enzyme electrode, 10% of vmax with 1-butanol, soluble enzyme, 5.5% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 82fold preferred over (R)-enantiomer, with soluble enzyme 315fold, respectively Comamonas testosteroni heptan-2-one + ferrocyanide - ? 407636 1.1.9.1 (R)-2-hexanol + ferrocyanide enzyme electrode, 14% of vmax with 1-butanol, soluble enzyme, 9% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 35fold preferred over (R)-enantiomer, with soluble enzyme 105fold, respectively Comamonas testosteroni hexan-2-one + ferricyanide - ? 407637 1.1.9.1 (R)-2-octanol + ferrocyanide enzyme electrode, 8% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 82fold preferred over (R)-enantiomer, with soluble enzyme 800fold, respectively Comamonas testosteroni octan-2-one + ferricyanide - ? 407640 1.1.9.1 (R)-3-bromo-2-methyl-1-propanol + ferricyanide - Comamonas testosteroni (R)-3-bromo-2-methyl-propanal + ferrocyanide - r 407642 1.1.9.1 (R)-glycidol + ferrocyanide enzyme electrode, 37% of vmax with 1-butanol, soluble enzyme, 1.6% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 0.9fold preferred over (R)-enantiomer, with soluble enzyme no distinction Comamonas testosteroni ? + ferricyanide - ? 407650 1.1.9.1 (R)-solketal + 2 ferricyanide (R)-enantiomer is prefered, enantiomeric ratio is 30 Comamonas testosteroni (R)-solketal aldehyde + 2 ferrocyanide + 2 H+ - r 407655 1.1.9.1 (R)-solketal + ferrocyanide enzyme electrode, 35% of vmax with 1-butanol, soluble enzyme, 10% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 39fold preferred over (R)-enantiomer, with soluble enzyme 117fold, respectively Comamonas testosteroni ? + ferricyanide - ? 407656 1.1.9.1 (R)-solketal aldehyde + ferrocyanide - Comamonas testosteroni (R)-solketal + ferricyanide - r 407657 1.1.9.1 (R)-solketal aldehyde + ferrocyanide (R)-enantiomer is prefered, enantiomeric ratio is 2.8 for initial rate measurement Comamonas testosteroni (R)-solketal + ferricyanide - r 407657 1.1.9.1 (S)-2-butanol + ferricyanide enzyme electrode, 14% of vmax with 1-butanol, soluble enzyme, 13% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 13fold preferred over (R)-enantiomer, with soluble enzyme 13.5fold, respectively Comamonas testosteroni butan-2-one + ferrocyanide - ? 407669 1.1.9.1 (S)-2-heptanol + ferricyanide enzyme electrode, 70% of vmax with 1-butanol, soluble enzyme, 63% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 82fold preferred over (R)-enantiomer, with soluble enzyme 315fold, respectively Comamonas testosteroni heptan-2-one + ferrocyanide - ? 407670 1.1.9.1 (S)-2-hexanol + ferrocyanide enzyme electrode, 74% of vmax with 1-butanol, soluble enzyme, 71% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 35fold preferred over (R)-enantiomer, with soluble enzyme 105fold, respectively Comamonas testosteroni hexan-2-one + ferricyanide - ? 407671 1.1.9.1 (S)-2-octanol + ferrocyanide enzyme electrode, 82% of vmax with 1-butanol, soluble enzyme, 80% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 82fold preferred over (R)-enantiomer, with soluble enzyme 800fold, respectively Comamonas testosteroni octan-2-one + ferricyanide - ? 407673 1.1.9.1 (S)-glycidol + ferrocyanide enzyme electrode, 30% of vmax with 1-butanol, soluble enzyme, 1.6% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 0.9fold preferred over (R)-enantiomer, with soluble enzyme no disticnction Comamonas testosteroni ? + ferricyanide - ? 407680 1.1.9.1 (S)-solketal + 2 ferricyanide the second step in (S)-solketal conversion is much faster than the first one and that opposite enantiomeric preferences exist for the alcohol and the aldehyde substrates. (R)-enantiomer is prefered, enantiomeric ratio is 30 for initial rate measurement, 117 for progress curve analysis Comamonas testosteroni (S)-solketal aldehyde + 2 ferrocyanide + 2 H+ - r 407685 1.1.9.1 (S)-solketal + ferrocyanide enzyme electrode, 9% of vmax with 1-butanol, 0.7% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 39fold preferred over (R)-enantiomer, with soluble enzyme 117fold, respectively Comamonas testosteroni ? + ferricyanide - ? 407686 1.1.9.1 1,2-butanediol + ferricyanide ADH IIB, 84% of the activity with 1-butanol. ADH IIG, 86% of the activity with 1,2-propanediol Pseudomonas putida 2-hydroxybutanal + ferrocyanide - ? 407692 1.1.9.1 1,2-cyclohexanediol + ferricyanide - Comamonas testosteroni 2-hydroxycyclohexanone + ferrocyanide - ? 407693 1.1.9.1 1,2-ethanediol + ferricyanide ADH IIB, 0% of the activity with 1-butanol. ADH IIG, 71% of the activity with 1,2-propanediol Pseudomonas putida hydroxyacetaldehyde + ferrocyanide - ? 407703 1.1.9.1 1,2-propanediol + ferricyanide ADH IIB, 43% of the activity with 1-butanol. ADH IIG, maximum activity Pseudomonas putida 2-hydroxypropanal + ferrocyanide - ? 407704 1.1.9.1 1,3-butanediol + ferricyanide ADH IIB, 105% of the activity with 1-butanol. ADH IIG, 75% of the activity with 1,2-propanediol Pseudomonas putida 3-hydroxybutanal + ferrocyanide - ? 407707 1.1.9.1 1,3-butanediol + ferricyanide enzyme electrode, 91% of vmax with 1-butanol Comamonas testosteroni ? + ferrocyanide - ? 407708 1.1.9.1 1,3-cyclohexanediol + ferricyanide - Comamonas testosteroni 3-hydroxycyclohexanone + ferrocyanide - ? 407709 1.1.9.1 1,3-propandione + ferricyanide ADH IIB, 61% of the activity with 1-butanol. ADH IIG, 42% of the activity with 1,2-propanediol Pseudomonas putida 3-hydroxypropanal + ferrocyanide - ? 407710 1.1.9.1 1,4-butanediol + ferricyanide ADH IIB, 110% of the activity with 1-butanol. ADH IIG, 75% of the activity with 1,2-propanediol Pseudomonas putida 4-hydroxybutanal + ferrocyanide - ? 407711 1.1.9.1 1-butanol + ferricyanide - Comamonas testosteroni butanal + ferrocyanide - ? 399267 1.1.9.1 1-butanol + ferricyanide ADH IIG, 42% of the activity with 1,2-propanediol Pseudomonas putida butanal + ferrocyanide - ? 399267 1.1.9.1 1-butanol + ferricyanide maximum velocity rate for both enzyme electrode and soluble enzyme Comamonas testosteroni butanal + ferrocyanide - ? 399267 1.1.9.1 1-butanol + N,N,N',N'-tetramethyl-p-phenylenediamine - Comamonas testosteroni butanal + ? - r 407717 1.1.9.1 1-heptanol + ferricyanide ADH IIB, 99% of the activity with 1-butanol. ADH IIG, 39% of the activity with 1,2-propanediol Pseudomonas putida heptanal + ferrocyanide - ? 407722 1.1.9.1 1-hexanol + ferricyanide ADH IIB, 70% of the activity with 1-butanol. ADH IIG, 46% of the activity with 1,2-propanediol Pseudomonas putida hexanal + ferrocyanide - ? 407723 1.1.9.1 1-octanol + ferricyanide - Comamonas testosteroni octanal + ferrocyanide - ? 407739 1.1.9.1 1-octanol + ferricyanide ADH IIB, 44% of the activity with 1-butanol. ADH IIG, 24% of the activity with 1,2-propanediol Pseudomonas putida octanal + ferrocyanide - ? 407739 1.1.9.1 1-octanol + ferrocyanide enzyme electrode, 95% of vmax with 1-butanol, soluble enzyme, 95% of vmax with 1-butanol Comamonas testosteroni octanal + ferricyanide - ? 407740 1.1.9.1 1-octanol + N,N,N',N'-tetramethyl-p-phenylenediamine - Comamonas testosteroni octanal + ? - r 407741 1.1.9.1 1-pentanol + ferricyanide - Comamonas testosteroni pentanal + ferrocyanide - ? 407754 1.1.9.1 1-pentanol + ferricyanide ADH IIB, 100% of the activity with 1-butanol. ADH IIG, 40% of the activity with 1,2-propanediol Pseudomonas putida pentanal + ferrocyanide - ? 407754 1.1.9.1 1-pentanol + ferrocyanide enzyme electrode, 93% of vmax with 1-butanol, soluble enzyme, 100% of vmax with 1-butanol Comamonas testosteroni pentanal + ferricyanide - ? 407755 1.1.9.1 1-pentanol + N,N,N',N'-tetramethyl-p-phenylenediamine - Comamonas testosteroni pentanal + ? - r 407756 1.1.9.1 1-propanol + ferricyanide - Comamonas testosteroni propanal + ferrocyanide - ? 407758 1.1.9.1 1-propanol + ferricyanide ADH IIB, 70% of the activity with 1-butanol. ADH IIG, 41% of the activity with 1,2-propanediol Pseudomonas putida propanal + ferrocyanide - ? 407758 1.1.9.1 1-propanol + ferricyanide enzyme electrode, 93% of vmax with 1-butanol, soluble enzyme, 90% of vmax with 1-butanol Comamonas testosteroni propanal + ferrocyanide - ? 407758 1.1.9.1 1-propanol + N,N,N',N'-tetramethyl-p-phenylenediamine - Comamonas testosteroni propanal + ? - r 407759 1.1.9.1 2,3-butanediol + ferricyanide ADH IIB, 12% of the activity with 1-butanol. ADH IIG, 71% of the activity with 1,2-propanediol Pseudomonas putida 3-hydroxybutan-2-one + ferrocyanide - ? 407818 1.1.9.1 2,4-pentanediol + ferricyanide ADH IIB, 45% of the activity with 1-butanol. ADH IIG, 0% of the activity with 1,2-propanediol Pseudomonas putida 4-hydroxypentan-2-one + ferrocyanide - ? 407829 1.1.9.1 2-butanol + ferricyanide - Comamonas testosteroni butanone + ferrocyanide - ? 407841 1.1.9.1 2-butanol + ferricyanide ADH IIB, 90% of the activity with 1-butanol. ADH IIG, 15% of the activity with 1,2-propanediol Pseudomonas putida butanone + ferrocyanide - ? 407841 1.1.9.1 2-butanol + ferrocyanide enzyme electrode, 17% of vmax with 1-butanol, soluble enzyme, 10% of vmax with 1-butanol Comamonas testosteroni butanone + ferricyanide - ? 407842 1.1.9.1 2-hexanol + ferricyanide ADH IIB, 99% of the activity with 1-butanol. ADH IIG, 33% of the activity with 1,2-propanediol Pseudomonas putida hexan-2-one + ferrocyanide - ? 399434 1.1.9.1 2-methylcyclohexanol + ferricyanide - Comamonas testosteroni 2-methylcyclohexanone + ferrocyanide - ? 407872 1.1.9.1 2-octanol + ferricyanide - Comamonas testosteroni octan-2-one + ferrocyanide - ? 407884 1.1.9.1 2-octanol + ferrocyanide enzyme electrode, 84% of vmax with 1-butanol, soluble enzyme, 80% of vmax with 1-butanol Comamonas testosteroni octan-2-one + ferricyanide - ? 407885 1.1.9.1 2-pentanol + ferricyanide - Comamonas testosteroni pentan-2-one + ferrocyanide - ? 399477 1.1.9.1 2-pentanol + ferricyanide ADH IIB, 120% of the activity with 1-butanol. ADH IIG, 19% of the activity with 1,2-propanediol Pseudomonas putida pentan-2-one + ferrocyanide - ? 399477 1.1.9.1 2-pentanol + ferrocyanide enzyme electrode, 40% of vmax with 1-butanol, soluble enzyme, 34% of vmax with 1-butanol Comamonas testosteroni pentan-2-one + ferricyanide - ? 407901 1.1.9.1 2-propanol + ferricyanide - Comamonas testosteroni acetone + ferrocyanide - ? 407904 1.1.9.1 2-propanol + ferricyanide ADH IIB, 52% of the activity with 1-butanol. ADH IIG, 0% of the activity with 1,2-propanediol Pseudomonas putida acetone + ferrocyanide - ? 407904 1.1.9.1 2-propanol + ferrocyanide enzyme electrode, 9% of vmax with 1-butanol, soluble enzyme, 6% of vmax with 1-butanol Comamonas testosteroni acetone + ferricyanide - ? 407905 1.1.9.1 3-heptanol + ferricyanide - Comamonas testosteroni heptan-3-one + ferrocyanide - ? 408001 1.1.9.1 3-heptanol + ferrocyanide enzyme electrode, 3.7% of vmax with 1-butanol, soluble enzyme, 1% of vmax with 1-butanol Comamonas testosteroni heptan-3-one + ferricyanide - ? 408002 1.1.9.1 3-hexanol + ferricyanide - Comamonas testosteroni hexan-3-one + ferrocyanide - ? 408003 1.1.9.1 3-hexanol + ferrocyanide enzyme electrode, 6.5% of vmax with 1-butanol, soluble enzyme, 9% of vmax with 1-butanol Comamonas testosteroni hexan-3-one + ferricyanide - ? 408004 1.1.9.1 3-methyl-2-pentanol + ferricyanide enzyme electrode, 3.7% of vmax with 1-butanol Comamonas testosteroni 3-methylpentan-2-one + ferrocyanide - ? 408019 1.1.9.1 3-methyl-2-pentanol + ferricyanide enzyme electrode, 9.3% of vmax with 1-butanol Comamonas testosteroni 3-methylpentan-2-one + ferrocyanide - ? 408019 1.1.9.1 3-methylcyclohexanol + ferricyanide - Comamonas testosteroni 3-methylcyclohexanone + ferrocyanide - ? 408021 1.1.9.1 3-nonanol + ferricyanide - Comamonas testosteroni nonan-3-one + ferrocyanide - ? 408032 1.1.9.1 3-nonanol + ferricyanide enzyme electrode, 4.7% of vmax with 1-butanol, soluble enzyme, 4.5% of vmax with 1-butanol Comamonas testosteroni nonan-3-one + ferrocyanide - ? 408032 1.1.9.1 3-octanol + ferricyanide - Comamonas testosteroni octan-3-one + ferrocyanide - ? 408034 1.1.9.1 3-octanol + ferrocyanide enzyme electrode, 10% of vmax with 1-butanol, soluble enzyme, 10% of vmax with 1-butanol Comamonas testosteroni octan-3-one + ferricyanide - ? 408035 1.1.9.1 3-pentanol + ferricyanide - Comamonas testosteroni pentan-3-one + ferrocyanide - ? 408036 1.1.9.1 3-pentanol + ferricyanide ADH IIB, 43% of the activity with 1-butanol. ADH IIG, 15% of the activity with 1,2-propanediol Pseudomonas putida pentan-3-one + ferrocyanide - ? 408036 1.1.9.1 3-pentanol + ferrocyanide enzyme electrode, 1% of vmax with 1-butanol, soluble enzyme, 1.4% of vmax with 1-butanol Comamonas testosteroni pentan-3-one + ferricyanide - ? 408037 1.1.9.1 4-decanol + ferricyanide - Comamonas testosteroni decan-4-one + ferrocyanide - ? 408085 1.1.9.1 4-decanol + ferricyanide enzyme electrode, 3.7% of vmax with 1-butanol, soluble enzyme, 3.6% of vmax with 1-butanol Comamonas testosteroni decan-4-one + ferrocyanide - ? 408085 1.1.9.1 4-heptanol + ferricyanide - Comamonas testosteroni heptan-4-one + ferrocyanide - ? 408091 1.1.9.1 4-heptanol + ferricyanide enzyme electrode, 9.3% of vmax with 1-butanol, soluble enzyme, 10% of vmax with 1-butanol Comamonas testosteroni heptan-4-one + ferrocyanide - ? 408091 1.1.9.1 4-methyl-2-pentanol + ferricyanide enzyme electrode, 5.6% of vmax with 1-butanol Comamonas testosteroni 4-methylpentan-2-one + ferrocyanide - ? 408108 1.1.9.1 5-methyl-2-hexanol + ferricyanide - Comamonas testosteroni 5-methylhexan-2-one + ferrocyanide - r 451158 1.1.9.1 6-aminohexan-1-ol + N,N,N',N'-tetramethyl-p-phenylenediamine - Comamonas testosteroni 6-aminohexanal + ? - r 408216 1.1.9.1 acetaldehyde + reduced N,N,N',N'-tetramethyl-p-phenylenediamine - Comamonas testosteroni ethanol + N,N,N',N'-tetramethyl-p-phenylenediamine - r 408248 1.1.9.1 allyl alcohol + ferricyanide ADH IIB, 160% of the activity with 1-butanol. ADH IIG, 46% of the activity with 1,2-propanediol Pseudomonas putida prop-2-enal + ferrocyanide - ? 408345 1.1.9.1 benzyl alcohol + N,N,N',N'-tetramethyl-p-phenylenediamine - Comamonas testosteroni benzaldehyde + ? - r 408769 1.1.9.1 butanal + ferrocyanide ADH IIB, 32% of the activity with 1-butanol. ADH IIG, 13% of the activity with 1,2-propanediol Pseudomonas putida 1-butanol + ferricyanide - ? 408792 1.1.9.1 butanal + reduced N,N,N',N'-tetramethyl-p-phenylenediamine - Comamonas testosteroni 1-butanol + N,N,N',N'-tetramethyl-p-phenylenediamine - r 408793 1.1.9.1 butane-1,3-diol + N,N,N',N'-tetramethyl-p-phenylenediamine - Comamonas testosteroni ? - r 408794 1.1.9.1 cyclohexanol + ferricyanide - Comamonas testosteroni cyclohexanal + ferrocyanide - ? 409109 1.1.9.1 cyclohexanol + ferricyanide enzyme electrode, 11% of vmax with 1-butanol, soluble enzyme, 9% of vmax with 1-butanol Comamonas testosteroni cyclohexanone + ferrocyanide - ? 409110 1.1.9.1 ethanal + ferrocyanide - Comamonas testosteroni ethanol + ferricyanide - r 409370 1.1.9.1 ethanal + ferrocyanide ADH IIB, 78% of the activity with 1-butanol. ADH IIG, 16% of the activity with 1,2-propanediol Pseudomonas putida ethanol + ferricyanide - ? 409370 1.1.9.1 ethanol + cytochrome c 5% of the activity with Wurster's Blue. In presence of phenazine methosulfate, 60% of the activity with Wurster's Blue Comamonas testosteroni ethanal + reduced cytochrome c - r 409373 1.1.9.1 ethanol + ferricyanide - Comamonas testosteroni ethanal + ferrocyanide - ? 409374 1.1.9.1 ethanol + ferricyanide - Comamonas testosteroni ethanal + ferrocyanide - r 409374 1.1.9.1 ethanol + ferricyanide 100% of the activity with Wurster's Blue Comamonas testosteroni ethanal + ferrocyanide - r 409374 1.1.9.1 ethanol + ferricyanide 86% of vmax with 1-butanol, soluble enzyme, 75% of vmax with 1-butanol Comamonas testosteroni ethanal + ferrocyanide - ? 409374 1.1.9.1 ethanol + ferricyanide ADH IIB, 78% of the activity with 1-butanol. ADH IIG, 22% of the activity with 1,2-propanediol Pseudomonas putida ethanal + ferrocyanide - ? 409374 1.1.9.1 ethanol + N,N,N',N'-tetramethyl-p-phenylenediamine i.e. Wurster's Blue Comamonas testosteroni ethanal + ? - ? 409375 1.1.9.1 ethanol + oxidized 2,6-dichlorophenolindophenol 10% of the activity with Wurster's Blue. In presence of phenazine methosulfate, 75% of the activity with Wurster's Blue Comamonas testosteroni ethanal + reduced 2,6-dichlorophenolindophenol - r 409372 1.1.9.1 formaldehyde + N,N,N',N'-tetramethyl-p-phenylenediamine - Comamonas testosteroni methanol + ? - r 409414 1.1.9.1 glycerol + ferrocyanide ADH IIB, 0% of the activity with 1-butanol. ADH IIG, 63% of the activity with 1,2-propanediol Pseudomonas putida 2,3-dihydroxypropanal + ferricyanide - ? 409539 1.1.9.1 iso-amyl alcohol + ferricyanide ADH IIB, 100% of the activity with 1-butanol. ADH IIG, 93% of the activity with 1,2-propanediol Pseudomonas putida 3-methylbutanol + ferrocyanide - ? 409605 1.1.9.1 additional information no substrate: D-glucose, D-galactose, D-fructose, Alpha-D-methylglucoside, mannitol Comamonas testosteroni ? - ? 89 1.1.9.1 additional information The affinity of the enzyme for aliphatic alcohols increases with the chain length of the substrate. The same property is observed for secondary alcohols in the series 2-propanol to 2-octanol Comamonas testosteroni ? - ? 89 1.1.9.1 n-butanol + ferricyanide - Comamonas testosteroni butanal + ferrocyanide - ? 392780 1.1.9.1 octanal + reduced N,N,N',N'-tetramethyl-p-phenylenediamine - Comamonas testosteroni 1-octanol + N,N,N',N'-tetramethyl-p-phenylenediamine - r 409988 1.1.9.1 propanal + ferrocyanide ADH IIB, 125% of the activity with 1-butanol. ADH IIG, 58% of the activity with 1,2-propanediol Pseudomonas putida 1-propanol + ferricyanide - ? 410112 1.1.9.1 rac-2,2-dimethyl-4-hydroxymethyl-1,3-dioxolane + ferricyanide i.e. solketal. Enantiomeric ratio is 30 for solketal and 6 for rac-2,2-dimethyl-4-[1,1-2H]hydroxymethyl-1,3-[5,5,4-2H]dioxolane, d5-solketal. Isotopic substitution affects the relative kinetic weights of the initial hydron/deuteron transfer from substrate to cofactor and the subsequent proton/deuteron shift in the cofactor-product complex Comamonas testosteroni ? + ferrocyanide - ? 374531