EC Number   |
General Information |
Reference |
|---|
   2.3.2.15 | evolution |
evolution and functional differentiation of the recently diverged three phytochelatin synthase genes from Arundo donax, phylogenetic analysis and evolutionary modeling, overview. AdPCS1-3 proteins are significantly less divergent than other duplicated PCSs, displaying only 25-29 substitutions. All the canonical features of PCSs are present, namely the catalytic triad Cys56, His162, and Asp180. The lengths of both N- and C-terminal domains are comparable to those of previously validated PCSs. AdPCS1-3 genes evolved at different evolutionary rates |
759571 |
| 2.3.2.15 | evolution |
genes encoding phytochelatin synthases have been found in all vascular plants as well as some algae, fungi, diatoms and invertebrates. Horizontal gene transfer of phytochelatin synthases from bacteria to extremophilic green algae. A detailed phylogenetic analysis gives insight into the complicated evolutionary history of PCS genes and provides evidence for multiple horizontal gene transfer events from bacteria to eukaryotes within the gene family. A separate subgroup containing PCS-like genes within the PCS gene family is not supported since the PCS genes are monophyletic only when the PCS-like genes are included. Genotyping, overview |
-, 759735 |
| 2.3.2.15 | evolution |
the two CDSs of OsPCS1 and OsPCS2 show 72% sequence identity at the nucleotide level, whereas the two proteins share overall 62.1% amino acid sequence identity. Moreover, both the intron sizes and nucleotide sequences are found to be highly differing between OsPCS1 and OsPCS2. Alignment of the deduced OsPCS1 and OsPCS2 polypeptides indicate the presence of N-terminal Phytochelatin (pfam05023) domain with high sequence identity (80.7%) including the conserved catalytic triad of Cys (C), His (H) and Asp (D). On the other hand, the C-terminal Phytochelatin_C (pfam09328) domain show moderate identity (57.68%) between these two polypeptides |
-, 759971 |
| 2.3.2.15 | malfunction |
AtPCS2-overexpressing transgenic Arabidopsis thaliana and Nicotiana tabacum plants display increased seed germination rates and seedling growth under high salt stress. In addition, transgenic Arabidopsis subjected to salt stress exhibit enhanced proline accumulation and reduced Na+/K+ ratios compared to wild-type plants. Effects of salt stress on seed germination and root elongation of AtPCS2-overexpressing transgenic plants, phenotype, overview |
-, 759667 |
| 2.3.2.15 | malfunction |
genetically engineered plants having their OsPCS1 expression silenced via RNA interference (OsPCS1 RNAi) show no significant difference phenotype compared to wild-type plants. Treatment with buthionine sulfoximine, an inhibitor of GSH biosynthesis, significantly decreases Cd and As tolerance of rice seedlings. Concentrations of thiol peptides in the roots of OsPCS RNAi plants grown under Cd stress, overview |
759634 |
| 2.3.2.15 | malfunction |
genetically engineered plants having their OsPCS2 expression silenced via RNA interference (OsPCS2 RNAi) contain less phytochelatins (PCs) and more glutathione (GSH), the substrate of PC synthesis by PCS, than wild-type plants. OsPCS2 RNAi plants are sensitive to As(III) stress, but Cd tolerance is little affected. Treatment with buthionine sulfoximine, an inhibitor of GSH biosynthesis, significantly decreases Cd and As tolerance of rice seedlings. Concentrations of thiol peptides in the roots of OsPCS RNAi plants grown under Cd stress, overview |
759634 |
| 2.3.2.15 | malfunction |
RNAi-mediated grain-specific silencing of OsPCS decreases cadmium accumulation in rice grain |
-, 759971 |
| 2.3.2.15 | malfunction |
the As(III)-sensitive phenotype of cad1-3 seedlings is completely rescued by the introduction of OsPCS1full variant, whereas growth of the plants expressing the other OsPCS variants is inhibited by As(III) as strongly as that of cad1-3. Similarly, OsPCS1full introduction complements the Cd-sensitive phenotype of cad1-3, while the other OsPCS1 variants do not. OsPCS1 mutant plants show increased sensitivity to Cd and As(III) stress |
759935 |
| 2.3.2.15 | malfunction |
the As(III)-sensitive phenotype of cad1-3 seedlings is completely rescued by the introduction of OsPCS1full variant, whereas growth of the plants expressing the other OsPCS1 variants is inhibited by As(III) as strongly as that of cad1-3. Similarly, OsPCS1full introduction complements the Cd-sensitive phenotype of cad1-3, while the other OsPCS1 variants do not |
759935 |
| 2.3.2.15 | malfunction |
the As(III)-sensitive phenotype of cad1-3 seedlings is completely rescued by the introduction of OsPCS1full variant, whereas growth of the plants expressing the other OsPCS1 variants is inhibited by As(III) as strongly as that of cad1-3. Similarly, OsPCS1full introduction complements the Cd-sensitive phenotype of cad1-3, while the other OsPCS1 variants do not. OsPCS1 mutant plants show increased sensitivity to Cd and As(III) stress |
759935 |
