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Literature summary for 2.3.2.15 extracted from

  • Shah, K.; King, G.D.; Jiang, H.
    A chromatin modulator sustains self-renewal and enables differentiation of postnatal neural stem and progenitor cells (2020), J. Mol. Cell Biol., 12, 4-16 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.2.15

Activating Compound

Activating Compound Comment Organism Structure
As3+ 70fold activation at 0.05 mM, recombinant MBP-tagged isozyme PCS1 Oryza sativa Japonica Group
Cd2+ 50fold activation at 0.05 mM, recombinant MBP-tagged isozyme PCS1 Oryza sativa Japonica Group
additional information isozyme OsPCS1 is more strongly activated by As3+ than by Cd2+, and it is vice versa for expression of OsPCS2 Oryza sativa Japonica Group

Cloned(Commentary)

Cloned (Comment) Organism
gene PCS1, DNA and amino acid sequence determination and analysis, sequence comparisons, different PCS1 splicing variants are detected, quantitative expression analysis, recombinant expression of MBP-tagged isozyme PCS1 Oryza sativa Japonica Group
gene PCS2, DNA and amino acid sequence determination and analysis, sequence comparisons, different PCS1 splicing variants are detected, quantitative expression analysis, recombinant expression of MBP-tagged isozyme PCS2 Oryza sativa Japonica Group

Protein Variants

Protein Variants Comment Organism
additional information silencing of OsPCS1 expression via RNA interference by OsPCS1 RNAi introduced into rice via Agrobacterium tumefaciens strain EHA105 transfection method. Phenotypes, overview Oryza sativa Japonica Group
additional information silencing of OsPCS2 expression via RNA interference by OsPCS2 RNAi introduced into rice via Agrobacterium tumefaciens strain EHA105 transfection method. Phenotypes, overview Oryza sativa Japonica Group

Metals/Ions

Metals/Ions Comment Organism Structure
As3+ 50fold activation at 0.05 mM, recombinant MBP-tagged isozyme PCS2 Oryza sativa Japonica Group
Cd2+ 70fold activation at 0.05 mM, recombinant MBP-tagged isozyme PCS2 Oryza sativa Japonica Group
additional information isozyme OsPCS2 is more strongly activated by Cd2+ than by As3+, and it is vice versa for expression of OsPCS1 Oryza sativa Japonica Group

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glutathione + [Glu(-Cys)]n-Gly Oryza sativa Japonica Group
-
 Gly + [Glu(-Cys)]n+1-Gly
-
 ?

Organism

Organism UniProt Comment Textmining
Oryza sativa Japonica Group A0A1Q2SVY7 cultivar Nipponbare
-
Oryza sativa Japonica Group Q0DI55 cultivar Nipponbare
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information the expression level of isozyyme OsPCS2 is higher than that of isozyme OsPCS1 in the shoots and roots of rice plants Oryza sativa Japonica Group
-
additional information the expression level of isozyyme OsPCS2 is higher than that of isozyme OsPCS1 in the shoots and roots of rice plants. The expression pattern of OsPCS1 splice variants is not changed by Cd or As stress in both shoots and roots Oryza sativa Japonica Group
-
root
-
 Oryza sativa Japonica Group
-
seedling
-
 Oryza sativa Japonica Group
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + [Glu(-Cys)]n-Gly
-
 Oryza sativa Japonica Group Gly + [Glu(-Cys)]n+1-Gly
-
 ?

Synonyms

Synonyms Comment Organism
OsPCS1
-
 Oryza sativa Japonica Group
OsPCS2
-
 Oryza sativa Japonica Group
PC synthase
-
 Oryza sativa Japonica Group
PCS
-
 Oryza sativa Japonica Group

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
 assay at Oryza sativa Japonica Group

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Oryza sativa Japonica Group

Expression

Organism Comment Expression
Oryza sativa Japonica Group the expression of OsPCS2 is not significantly affected by As(III) stress in the roots additional information
Oryza sativa Japonica Group the expression pattern of OsPCS1 splice variants is not changed by Cd or As stress in both shoots and roots additional information
Oryza sativa Japonica Group expression of OsPCS1 is upregulated by As(III) stress in the roots up

General Information

General Information Comment Organism
malfunction genetically engineered plants having their OsPCS1 expression silenced via RNA interference (OsPCS1 RNAi) show no significant difference phenotype compared to wild-type plants. Treatment with buthionine sulfoximine, an inhibitor of GSH biosynthesis, significantly decreases Cd and As tolerance of rice seedlings. Concentrations of thiol peptides in the roots of OsPCS RNAi plants grown under Cd stress, overview Oryza sativa Japonica Group
malfunction genetically engineered plants having their OsPCS2 expression silenced via RNA interference (OsPCS2 RNAi) contain less phytochelatins (PCs) and more glutathione (GSH), the substrate of PC synthesis by PCS, than wild-type plants. OsPCS2 RNAi plants are sensitive to As(III) stress, but Cd tolerance is little affected. Treatment with buthionine sulfoximine, an inhibitor of GSH biosynthesis, significantly decreases Cd and As tolerance of rice seedlings. Concentrations of thiol peptides in the roots of OsPCS RNAi plants grown under Cd stress, overview Oryza sativa Japonica Group
physiological function isozyme OsPCS2 is a major isozyme controlling phytochelatin (PC) synthesis, and PCs are important for As tolerance in rice. PC synthesis may make a smaller contribution to Cd tolerance in rice. Synthesis of thiol peptides in response to Cd or As(III) stress in rice roots Oryza sativa Japonica Group
physiological function pyhtochelatins (PCs) are important for As tolerance in rice. PC synthesis may make a smaller contribution to Cd tolerance in rice. Synthesis of thiol peptides in response to Cd or As(III) stress in rice roots Oryza sativa Japonica Group