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Literature summary for 2.3.2.15 extracted from
- Evolution and functional differentiation of recently diverged phytochelatin synthase genes from Arundo donax L. (2019), J. Exp. Bot., 70, 5391-5405 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene PCS1, sequence comparisons and phylogenetic analysis, recombinant expression in Arabidopsis thaliana Col-0 ecotype, and induction by Cd2+, functional recombinant expression in Saccharomyces cerevisiae YK44 highly Cd2+-sensitive strain conferring Cd resistance. Compared to PCS2 and 3 expressing lines, the AdPCS1 transformant has a longer lag phase, resulting in slower initial growth. This difference is rescued after 24 h, with the growth kinetics of the AdPCS1 transformant becoming normal, indicating that the difference results from decreased translational efficiency | Arundo donax |
| gene PCS2, sequence comparisons and phylogenetic analysis, recombinant expression in Arabidopsis thaliana Col-0 ecotype, and induction by Cd2+, functional recombinant expression in Saccharomyces cerevisiae YK44 highly Cd-sensitive strain conferring Cd2+ resistance | Arundo donax |
| gene PCS3, sequence comparisons and phylogenetic analysis, recombinant expression in Arabidopsis thaliana Col-0 ecotype, and induction by Cd2+, functional recombinant expression in Saccharomyces cerevisiae YK44 highly Cd-sensitive strain conferring Cd2+ resistance | Arundo donax |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + [Glu(-Cys)]n-Gly | Arundo donax | - |
Gly + [Glu(-Cys)]n+1-Gly | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arundo donax | A0A4Y5QLU9 | - |
- |
| Arundo donax | A0A4Y5QLV0 | - |
- |
| Arundo donax | A0A4Y5QPE2 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| internode | - |
Arundo donax | - |
| leaf | sheath and blade | Arundo donax | - |
| additional information | expression pattern of isozymes AdPCS1-3 in different organs/tissues of Arundo donax, semi-quantitative RT-PCR expression analysis, overview | Arundo donax | - |
| node | - |
Arundo donax | - |
| rhizome | - |
Arundo donax | - |
| root | - |
Arundo donax | - |
| shoot | - |
Arundo donax | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + Glu-Cys-Gly | - |
Arundo donax | Gly + [Glu(-Cys)]2-Gly | - |
? | |
| glutathione + [Glu(-Cys)]2-Gly | - |
Arundo donax | Gly + [Glu(-Cys)]3-Gly | - |
? | |
| glutathione + [Glu(-Cys)]3-Gly | - |
Arundo donax | Gly + [Glu(-Cys)]4-Gly | - |
? | |
| glutathione + [Glu(-Cys)]n-Gly | - |
Arundo donax | Gly + [Glu(-Cys)]n+1-Gly | - |
? | |
| additional information | PC2 is the main polymerization product of PCS1, followed by PC3, low amount of PC4 formation. PCS1 has the highest activity of the three isozymes, PCS3 the lowest | Arundo donax | ? | - |
- |
|
| additional information | PC2 is the main polymerization product of PCS2, followed by PC3, low amount of PC4 formation. PCS1 has the highest activity of the three isozymes, PCS3 the lowest | Arundo donax | ? | - |
- |
|
| additional information | PC2 is the main polymerization product of PCS3, followed by PC3, low amount of PC4 formation. PCS1 has the highest activity of the three isozymes, PCS3 the lowest | Arundo donax | ? | - |
- |
|
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AdPCS1 | - |
Arundo donax |
| AdPCS2 | - |
Arundo donax |
| AdPCS3 | - |
Arundo donax |
| PCS | - |
Arundo donax |
| phytochelatin synthase | - |
Arundo donax |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Arundo donax | expression of gene AdPCS 1 is responsive to stress through heavy metals | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | evolution and functional differentiation of the recently diverged three phytochelatin synthase genes from Arundo donax, phylogenetic analysis and evolutionary modeling, overview. AdPCS1-3 proteins are significantly less divergent than other duplicated PCSs, displaying only 25-29 substitutions. All the canonical features of PCSs are present, namely the catalytic triad Cys56, His162, and Asp180. The lengths of both N- and C-terminal domains are comparable to those of previously validated PCSs. AdPCS1-3 genes evolved at different evolutionary rates | Arundo donax |
| physiological function | phytochelatin synthases (PCSs) play pivotal roles in the detoxification of heavy metals and metalloids in plants | Arundo donax |
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