EC Number   |
Reference |
|---|
   1.1.2.7 | - |
667721 |
| 1.1.2.7 | Ca2+-containing and Ca2+-free enzymes from cell-free extracts by ion exchange and hydrophobic interaction chromatography |
673048 |
| 1.1.2.7 | native active alpha2beta2 MDH complex by ultracentrifugation, anion echange chromatgraphy, and gel filtration |
723854 |
| 1.1.2.7 | native enzyme 22fold from strain AM1 in a single cation exchange chromatographical step, followed by ultrafiltration and buffer exchange, over 97% purity |
670785 |
| 1.1.2.7 | native enzyme 9fold to homogeneity by anion exchange chromatography, ammonium sulfate fractionation, and hydrophobic interaction chromatography, followed by ultrafiltration and gel filtration |
687432 |
| 1.1.2.7 | native isozymes by anion exchange chromatography and gel filtration, type I 7.9fold, type II 14.7fold, the lysozyme and freeze-thawing cell disruption method significantly increases the amount of type II MDH in the soluble fraction compared with strong physical disruption methods such as sonication and French Press |
725300 |
| 1.1.2.7 | recombinant selennomethionine-labeled, detagged MxaJ(residues 12-281) without signal peptide from Escherichia coli strain BL21(DE3) by anion-exchange chromatography and gel filtration |
763672 |
| 1.1.2.7 | soluble fraction concentrated with Centricon (Millipore, Billerica, Mass, USA), applied to a POROS 20 HQ column, followed by FPLC Superose 12 HR 10/30 column |
753048 |
