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Comparative analysis of two types of methanol dehydrogenase from Methylophaga aminisulfidivorans MPT grown on methanol

Kim, H.G.; Han, G.H.; Kim, D.; Choi, J.S.; Kim, S.W.; J. Basic Microbiol. 52, 141-149 (2012)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
mxaFJGIR gene cluster encoding the large and small subunits, and protein mxaJ, organized in an operon and are transcribed as a single mRNA, DNA and amino acid sequence determination and analysis; mxaFJGIR gene cluster encoding the large and small subunits, and protein mxaJ, organized in an operon and are transcribed as a single mRNA, DNA and amino acid sequence determination and analysis
Methylophaga aminisulfidivorans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.013
-
methanol
type II MDH, pH 7.0, 30°C, with cytochrome cL; type II MDH, pH 7.0, 30°C, with cytochrome cL
Methylophaga aminisulfidivorans
0.0503
-
methanol
type I MDH, pH 7.0, 30°C, with cytochrome cL; type I MDH, pH 7.0, 30°C, with cytochrome cL
Methylophaga aminisulfidivorans
0.0511
-
methanol
type II MDH, pH 9.0, 30°C, with phenazine ethosulfate and 2,6-dichlorophenolindophenol; type II MDH, pH 9.0, 30°C, with phenazine ethosulfate and 2,6-dichlorophenolindophenol
Methylophaga aminisulfidivorans
0.1046
-
methanol
type I MDH, pH 9.0, 30°C, with phenazine ethosulfate and 2,6-dichlorophenolindophenol; type I MDH, pH 9.0, 30°C, with phenazine ethosulfate and 2,6-dichlorophenolindophenol
Methylophaga aminisulfidivorans
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
periplasm
;
Methylophaga aminisulfidivorans
-
-
soluble
;
Methylophaga aminisulfidivorans
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
7580
-
2 * 65980, subunit alpha, + 2 * 7580, subunit beta, + 1 * 27860, MxaJ protein, type II MDH consists of two identical dimers of alpha and beta subunits organized to form the alpha2beta2 tetramer, and contains an additional MxaJ protein; 2 * 65980, subunit alpha, + 2 * 7580, subunit beta, + 1 * 27860, MxaJ protein, type II MDH consists of two identical dimers of alpha and beta subunits organized to form the alpha2beta2 tetramer, and contains an additional MxaJ protein; 2 * 65980, subunit alpha, + 2 * 7580, subunit beta, type I MDH consists of two identical dimers of alpha and beta subunits organized to form the alpha2beta2 tetramer; 2 * 65980, subunit alpha, + 2 * 7580, subunit beta, type I MDH consists of two identical dimers of alpha and beta subunits organized to form the alpha2beta2 tetramer
Methylophaga aminisulfidivorans
27860
-
2 * 65980, subunit alpha, + 2 * 7580, subunit beta, + 1 * 27860, MxaJ protein, type II MDH consists of two identical dimers of alpha and beta subunits organized to form the alpha2beta2 tetramer, and contains an additional MxaJ protein; 2 * 65980, subunit alpha, + 2 * 7580, subunit beta, + 1 * 27860, MxaJ protein, type II MDH consists of two identical dimers of alpha and beta subunits organized to form the alpha2beta2 tetramer, and contains an additional MxaJ protein
Methylophaga aminisulfidivorans
65980
-
2 * 65980, subunit alpha, + 2 * 7580, subunit beta, + 1 * 27860, MxaJ protein, type II MDH consists of two identical dimers of alpha and beta subunits organized to form the alpha2beta2 tetramer, and contains an additional MxaJ protein; 2 * 65980, subunit alpha, + 2 * 7580, subunit beta, + 1 * 27860, MxaJ protein, type II MDH consists of two identical dimers of alpha and beta subunits organized to form the alpha2beta2 tetramer, and contains an additional MxaJ protein; 2 * 65980, subunit alpha, + 2 * 7580, subunit beta, type I MDH consists of two identical dimers of alpha and beta subunits organized to form the alpha2beta2 tetramer; 2 * 65980, subunit alpha, + 2 * 7580, subunit beta, type I MDH consists of two identical dimers of alpha and beta subunits organized to form the alpha2beta2 tetramer
Methylophaga aminisulfidivorans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
methanol + 2 cytochrome cL
Methylophaga aminisulfidivorans
-
formaldehyde + 2 reduced cytochrome cL
-
-
?
methanol + 2 cytochrome cL
Methylophaga aminisulfidivorans MPT
-
formaldehyde + 2 reduced cytochrome cL
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Methylophaga aminisulfidivorans
A3FJ49
gene mxaJ; mxaFJGIR gene cluster encoding the large and small subunits, and protein mxaJ, organized in an operon and are transcribed as a single mRNA
-
Methylophaga aminisulfidivorans
A3FJ51
small subunit; mxaFJGIR gene cluster encoding the large and small subunits, and protein mxaJ, organized in an operon and are transcribed as a single mRNA
-
Methylophaga aminisulfidivorans MPT
A3FJ49
gene mxaJ; mxaFJGIR gene cluster encoding the large and small subunits, and protein mxaJ, organized in an operon and are transcribed as a single mRNA
-
Methylophaga aminisulfidivorans MPT
A3FJ51
small subunit; mxaFJGIR gene cluster encoding the large and small subunits, and protein mxaJ, organized in an operon and are transcribed as a single mRNA
-
Purification (Commentary)
Commentary
Organism
native isozymes by anion exchange chromatography and gel filtration, type I 7.9fold, type II 14.7fold, the lysozyme and freeze-thawing cell disruption method significantly increases the amount of type II MDH in the soluble fraction compared with strong physical disruption methods such as sonication and French Press; native isozymes by anion exchange chromatography and gel filtration, type I 7.9fold, type II 14.7fold, the lysozyme and freeze-thawing cell disruption method significantly increases the amount of type II MDH in the soluble fraction compared with strong physical disruption methods such as sonication and French Press
Methylophaga aminisulfidivorans
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.69
-
type I MDH, pH 7.0, 30°C, with cytochrome cL; type I MDH, pH 7.0, 30°C, with cytochrome cL
Methylophaga aminisulfidivorans
0.75
-
type II MDH, pH 7.0, 30°C, with cytochrome cL; type II MDH, pH 7.0, 30°C, with cytochrome cL
Methylophaga aminisulfidivorans
18.66
-
type I MDH, pH 9.0, 30°C, with phenazine ethosulfate and 2,6-dichlorophenolindophenol; type I MDH, pH 9.0, 30°C, with phenazine ethosulfate and 2,6-dichlorophenolindophenol
Methylophaga aminisulfidivorans
21.03
-
type II MDH, pH 9.0, 30°C, with phenazine ethosulfate and 2,6-dichlorophenolindophenol; type II MDH, pH 9.0, 30°C, with phenazine ethosulfate and 2,6-dichlorophenolindophenol
Methylophaga aminisulfidivorans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
methanol + 2 2,6-dichlorophenolindophenol
in the activity assay phenazine ethosulfate is used as primary electron acceptor, and 2,6-dichlorophenol indophenol as a terminal acceptor
725300
Methylophaga aminisulfidivorans
formaldehyde + 2 reduced 2,6-dichlorophenolindophenol
-
-
-
?
methanol + 2 2,6-dichlorophenolindophenol
in the activity assay phenazine ethosulfate is used as primary electron acceptor, and 2,6-dichlorophenolindophenol as a terminal acceptor
725300
Methylophaga aminisulfidivorans
formaldehyde + 2 reduced 2,6-dichlorophenolindophenol
-
-
-
?
methanol + 2 2,6-dichlorophenolindophenol
in the activity assay phenazine ethosulfate is used as primary electron acceptor, and 2,6-dichlorophenol indophenol as a terminal acceptor
725300
Methylophaga aminisulfidivorans MPT
formaldehyde + 2 reduced 2,6-dichlorophenolindophenol
-
-
-
?
methanol + 2 2,6-dichlorophenolindophenol
in the activity assay phenazine ethosulfate is used as primary electron acceptor, and 2,6-dichlorophenolindophenol as a terminal acceptor
725300
Methylophaga aminisulfidivorans MPT
formaldehyde + 2 reduced 2,6-dichlorophenolindophenol
-
-
-
?
methanol + 2 cytochrome cL
-
725300
Methylophaga aminisulfidivorans
formaldehyde + 2 reduced cytochrome cL
-
-
-
?
methanol + 2 cytochrome cL
native enzyme with pyrroloquinoline quinone as a prosthetic group and cytochrome cL as the primary electron acceptor
725300
Methylophaga aminisulfidivorans
formaldehyde + 2 reduced cytochrome cL
-
-
-
?
methanol + 2 cytochrome cL
-
725300
Methylophaga aminisulfidivorans MPT
formaldehyde + 2 reduced cytochrome cL
-
-
-
?
methanol + 2 cytochrome cL
native enzyme with pyrroloquinoline quinone as a prosthetic group and cytochrome cL as the primary electron acceptor
725300
Methylophaga aminisulfidivorans MPT
formaldehyde + 2 reduced cytochrome cL
-
-
-
?
Subunits
Subunits
Commentary
Organism
More
the original conformation of the MDH Methylophaga aminisulfidivorans MPT is most likely the alpha2beta2-MxaJ complex; the original conformation of the MDH Methylophaga aminisulfidivorans MPT is most likely the alpha2beta2-MxaJ complex
Methylophaga aminisulfidivorans
pentamer
2 * 65980, subunit alpha, + 2 * 7580, subunit beta, + 1 * 27860, MxaJ protein, type II MDH consists of two identical dimers of alpha and beta subunits organized to form the alpha2beta2 tetramer, and contains an additional MxaJ protein; 2 * 65980, subunit alpha, + 2 * 7580, subunit beta, + 1 * 27860, MxaJ protein, type II MDH consists of two identical dimers of alpha and beta subunits organized to form the alpha2beta2 tetramer, and contains an additional MxaJ protein
Methylophaga aminisulfidivorans
tetramer
2 * 65980, subunit alpha, + 2 * 7580, subunit beta, type I MDH consists of two identical dimers of alpha and beta subunits organized to form the alpha2beta2 tetramer; 2 * 65980, subunit alpha, + 2 * 7580, subunit beta, type I MDH consists of two identical dimers of alpha and beta subunits organized to form the alpha2beta2 tetramer
Methylophaga aminisulfidivorans
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
;
Methylophaga aminisulfidivorans
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.11
-
methanol
type I MDH, pH 9.0, 30°C, with phenazine ethosulfate and 2,6-dichlorophenolindophenol; type I MDH, pH 9.0, 30°C, with phenazine ethosulfate and 2,6-dichlorophenolindophenol
Methylophaga aminisulfidivorans
0.16
-
methanol
type II MDH, pH 9.0, 30°C, with phenazine ethosulfate and 2,6-dichlorophenolindophenol; type II MDH, pH 9.0, 30°C, with phenazine ethosulfate and 2,6-dichlorophenolindophenol
Methylophaga aminisulfidivorans
2.96
-
methanol
type I MDH, pH 7.0, 30°C, with cytochrome cL; type I MDH, pH 7.0, 30°C, with cytochrome cL
Methylophaga aminisulfidivorans
4.51
-
methanol
type II MDH, pH 7.0, 30°C, with cytochrome cL; type II MDH, pH 7.0, 30°C, with cytochrome cL
Methylophaga aminisulfidivorans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
;
Methylophaga aminisulfidivorans
Cofactor
Cofactor
Commentary
Organism
Structure
pyrroloquinoline quinone
;
Methylophaga aminisulfidivorans
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Methylophaga aminisulfidivorans
type I MDH; type I MDH
-
5.4
Methylophaga aminisulfidivorans
type II MDH; type II MDH
-
5.8
Cloned(Commentary) (protein specific)
Commentary
Organism
mxaFJGIR gene cluster encoding the large and small subunits, and protein mxaJ, organized in an operon and are transcribed as a single mRNA, DNA and amino acid sequence determination and analysis
Methylophaga aminisulfidivorans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyrroloquinoline quinone
-
Methylophaga aminisulfidivorans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.013
-
methanol
type II MDH, pH 7.0, 30°C, with cytochrome cL
Methylophaga aminisulfidivorans
0.0503
-
methanol
type I MDH, pH 7.0, 30°C, with cytochrome cL
Methylophaga aminisulfidivorans
0.0511
-
methanol
type II MDH, pH 9.0, 30°C, with phenazine ethosulfate and 2,6-dichlorophenolindophenol
Methylophaga aminisulfidivorans
0.1046
-
methanol
type I MDH, pH 9.0, 30°C, with phenazine ethosulfate and 2,6-dichlorophenolindophenol
Methylophaga aminisulfidivorans
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
periplasm
-
Methylophaga aminisulfidivorans
-
-
soluble
-
Methylophaga aminisulfidivorans
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
7580
-
2 * 65980, subunit alpha, + 2 * 7580, subunit beta, + 1 * 27860, MxaJ protein, type II MDH consists of two identical dimers of alpha and beta subunits organized to form the alpha2beta2 tetramer, and contains an additional MxaJ protein; 2 * 65980, subunit alpha, + 2 * 7580, subunit beta, type I MDH consists of two identical dimers of alpha and beta subunits organized to form the alpha2beta2 tetramer
Methylophaga aminisulfidivorans
27860
-
2 * 65980, subunit alpha, + 2 * 7580, subunit beta, + 1 * 27860, MxaJ protein, type II MDH consists of two identical dimers of alpha and beta subunits organized to form the alpha2beta2 tetramer, and contains an additional MxaJ protein
Methylophaga aminisulfidivorans
65980
-
2 * 65980, subunit alpha, + 2 * 7580, subunit beta, + 1 * 27860, MxaJ protein, type II MDH consists of two identical dimers of alpha and beta subunits organized to form the alpha2beta2 tetramer, and contains an additional MxaJ protein; 2 * 65980, subunit alpha, + 2 * 7580, subunit beta, type I MDH consists of two identical dimers of alpha and beta subunits organized to form the alpha2beta2 tetramer
Methylophaga aminisulfidivorans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
methanol + 2 cytochrome cL
Methylophaga aminisulfidivorans
-
formaldehyde + 2 reduced cytochrome cL
-
-
?
methanol + 2 cytochrome cL
Methylophaga aminisulfidivorans MPT
-
formaldehyde + 2 reduced cytochrome cL
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
native isozymes by anion exchange chromatography and gel filtration, type I 7.9fold, type II 14.7fold, the lysozyme and freeze-thawing cell disruption method significantly increases the amount of type II MDH in the soluble fraction compared with strong physical disruption methods such as sonication and French Press
Methylophaga aminisulfidivorans
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.69
-
type I MDH, pH 7.0, 30°C, with cytochrome cL
Methylophaga aminisulfidivorans
0.75
-
type II MDH, pH 7.0, 30°C, with cytochrome cL
Methylophaga aminisulfidivorans
18.66
-
type I MDH, pH 9.0, 30°C, with phenazine ethosulfate and 2,6-dichlorophenolindophenol
Methylophaga aminisulfidivorans
21.03
-
type II MDH, pH 9.0, 30°C, with phenazine ethosulfate and 2,6-dichlorophenolindophenol
Methylophaga aminisulfidivorans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
methanol + 2 2,6-dichlorophenolindophenol
in the activity assay phenazine ethosulfate is used as primary electron acceptor, and 2,6-dichlorophenol indophenol as a terminal acceptor
725300
Methylophaga aminisulfidivorans
formaldehyde + 2 reduced 2,6-dichlorophenolindophenol
-
-
-
?
methanol + 2 2,6-dichlorophenolindophenol
in the activity assay phenazine ethosulfate is used as primary electron acceptor, and 2,6-dichlorophenolindophenol as a terminal acceptor
725300
Methylophaga aminisulfidivorans
formaldehyde + 2 reduced 2,6-dichlorophenolindophenol
-
-
-
?
methanol + 2 2,6-dichlorophenolindophenol
in the activity assay phenazine ethosulfate is used as primary electron acceptor, and 2,6-dichlorophenol indophenol as a terminal acceptor
725300
Methylophaga aminisulfidivorans MPT
formaldehyde + 2 reduced 2,6-dichlorophenolindophenol
-
-
-
?
methanol + 2 2,6-dichlorophenolindophenol
in the activity assay phenazine ethosulfate is used as primary electron acceptor, and 2,6-dichlorophenolindophenol as a terminal acceptor
725300
Methylophaga aminisulfidivorans MPT
formaldehyde + 2 reduced 2,6-dichlorophenolindophenol
-
-
-
?
methanol + 2 cytochrome cL
-
725300
Methylophaga aminisulfidivorans
formaldehyde + 2 reduced cytochrome cL
-
-
-
?
methanol + 2 cytochrome cL
native enzyme with pyrroloquinoline quinone as a prosthetic group and cytochrome cL as the primary electron acceptor
725300
Methylophaga aminisulfidivorans
formaldehyde + 2 reduced cytochrome cL
-
-
-
?
methanol + 2 cytochrome cL
-
725300
Methylophaga aminisulfidivorans MPT
formaldehyde + 2 reduced cytochrome cL
-
-
-
?
methanol + 2 cytochrome cL
native enzyme with pyrroloquinoline quinone as a prosthetic group and cytochrome cL as the primary electron acceptor
725300
Methylophaga aminisulfidivorans MPT
formaldehyde + 2 reduced cytochrome cL
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
More
the original conformation of the MDH Methylophaga aminisulfidivorans MPT is most likely the alpha2beta2-MxaJ complex
Methylophaga aminisulfidivorans
pentamer
2 * 65980, subunit alpha, + 2 * 7580, subunit beta, + 1 * 27860, MxaJ protein, type II MDH consists of two identical dimers of alpha and beta subunits organized to form the alpha2beta2 tetramer, and contains an additional MxaJ protein
Methylophaga aminisulfidivorans
tetramer
2 * 65980, subunit alpha, + 2 * 7580, subunit beta, type I MDH consists of two identical dimers of alpha and beta subunits organized to form the alpha2beta2 tetramer
Methylophaga aminisulfidivorans
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Methylophaga aminisulfidivorans
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.11
-
methanol
type I MDH, pH 9.0, 30°C, with phenazine ethosulfate and 2,6-dichlorophenolindophenol
Methylophaga aminisulfidivorans
0.16
-
methanol
type II MDH, pH 9.0, 30°C, with phenazine ethosulfate and 2,6-dichlorophenolindophenol
Methylophaga aminisulfidivorans
2.96
-
methanol
type I MDH, pH 7.0, 30°C, with cytochrome cL
Methylophaga aminisulfidivorans
4.51
-
methanol
type II MDH, pH 7.0, 30°C, with cytochrome cL
Methylophaga aminisulfidivorans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Methylophaga aminisulfidivorans
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Methylophaga aminisulfidivorans
type I MDH
-
5.4
Methylophaga aminisulfidivorans
type II MDH
-
5.8
General Information
General Information
Commentary
Organism
additional information
type II MDH has higher specific activity than type I MDH. The original conformation of the MDH Methylophaga aminisulfidivorans MPT is most likely the alpha2beta2-MxaJ complex. The lysozyme and freeze-thawing cell disruption method significantly increases the amount of type II MDH in the soluble fraction compared with strong physical disruption methods such as sonication and French Press; type II MDH has higher specific activity than type I MDH. The original conformation of the MDH Methylophaga aminisulfidivorans MPT is most likely the alpha2beta2-MxaJ complex. The lysozyme and freeze-thawing cell disruption method significantly increases the amount of type II MDH in the soluble fraction compared with strong physical disruption methods such as sonication and French Press
Methylophaga aminisulfidivorans
General Information (protein specific)
General Information
Commentary
Organism
additional information
type II MDH has higher specific activity than type I MDH. The original conformation of the MDH Methylophaga aminisulfidivorans MPT is most likely the alpha2beta2-MxaJ complex. The lysozyme and freeze-thawing cell disruption method significantly increases the amount of type II MDH in the soluble fraction compared with strong physical disruption methods such as sonication and French Press
Methylophaga aminisulfidivorans
Other publictions for EC 1.1.2.7
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724402
Gvozdev
Quinone-dependent alcohol dehy ...
Diplococcus sp., Methylophilus methylotrophus, Methylophilus methylotrophus W3A1, Methylorubrum extorquens, Paracoccus denitrificans, Paracoccus pantotrophus, Pseudomonas sp., Rhodoblastus acidophilus
Biochemistry
77
843-856
2012
-
-
-
-
-
-
-
-
14
7
-
8
-
45
-
-
-
7
-
-
-
-
24
7
-
-
-
-
-
-
-
7
-
-
-
-
-
-
7
-
-
-
-
-
-
-
14
7
-
8
-
-
-
-
-
-
-
-
24
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
725300
Kim
Comparative analysis of two ty ...
Methylophaga aminisulfidivorans, Methylophaga aminisulfidivorans MPT
J. Basic Microbiol.
52
141-149
2012
-
-
1
-
-
-
-
4
2
-
3
4
-
7
-
-
1
-
-
-
4
-
12
3
1
-
-
4
1
-
-
1
-
2
-
-
-
2
2
-
-
-
-
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