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Literature summary for 1.1.2.7 extracted from
- Comparative analysis of two types of methanol dehydrogenase from Methylophaga aminisulfidivorans MPT grown on methanol (2012), J. Basic Microbiol., 52, 141-149.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| mxaFJGIR gene cluster encoding the large and small subunits, and protein mxaJ, organized in an operon and are transcribed as a single mRNA, DNA and amino acid sequence determination and analysis | Methylophaga aminisulfidivorans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.013 | - |
methanol | type II MDH, pH 7.0, 30°C, with cytochrome cL | Methylophaga aminisulfidivorans |  |
| 0.0503 | - |
methanol | type I MDH, pH 7.0, 30°C, with cytochrome cL | Methylophaga aminisulfidivorans | |
| 0.0511 | - |
methanol | type II MDH, pH 9.0, 30°C, with phenazine ethosulfate and 2,6-dichlorophenolindophenol | Methylophaga aminisulfidivorans | |
| 0.1046 | - |
methanol | type I MDH, pH 9.0, 30°C, with phenazine ethosulfate and 2,6-dichlorophenolindophenol | Methylophaga aminisulfidivorans | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| periplasm | - |
Methylophaga aminisulfidivorans | - |
- |
| soluble | - |
Methylophaga aminisulfidivorans | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 7580 | - |
2 * 65980, subunit alpha, + 2 * 7580, subunit beta, + 1 * 27860, MxaJ protein, type II MDH consists of two identical dimers of alpha and beta subunits organized to form the alpha2beta2 tetramer, and contains an additional MxaJ protein | Methylophaga aminisulfidivorans |
| 7580 | - |
2 * 65980, subunit alpha, + 2 * 7580, subunit beta, type I MDH consists of two identical dimers of alpha and beta subunits organized to form the alpha2beta2 tetramer | Methylophaga aminisulfidivorans |
| 27860 | - |
2 * 65980, subunit alpha, + 2 * 7580, subunit beta, + 1 * 27860, MxaJ protein, type II MDH consists of two identical dimers of alpha and beta subunits organized to form the alpha2beta2 tetramer, and contains an additional MxaJ protein | Methylophaga aminisulfidivorans |
| 65980 | - |
2 * 65980, subunit alpha, + 2 * 7580, subunit beta, + 1 * 27860, MxaJ protein, type II MDH consists of two identical dimers of alpha and beta subunits organized to form the alpha2beta2 tetramer, and contains an additional MxaJ protein | Methylophaga aminisulfidivorans |
| 65980 | - |
2 * 65980, subunit alpha, + 2 * 7580, subunit beta, type I MDH consists of two identical dimers of alpha and beta subunits organized to form the alpha2beta2 tetramer | Methylophaga aminisulfidivorans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methanol + 2 cytochrome cL | Methylophaga aminisulfidivorans | - |
formaldehyde + 2 reduced cytochrome cL | - |
? | |
| methanol + 2 cytochrome cL | Methylophaga aminisulfidivorans MPT | - |
formaldehyde + 2 reduced cytochrome cL | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methylophaga aminisulfidivorans | A3FJ49 | gene mxaJ; mxaFJGIR gene cluster encoding the large and small subunits, and protein mxaJ, organized in an operon and are transcribed as a single mRNA | - |
| Methylophaga aminisulfidivorans | A3FJ51 | small subunit; mxaFJGIR gene cluster encoding the large and small subunits, and protein mxaJ, organized in an operon and are transcribed as a single mRNA | - |
| Methylophaga aminisulfidivorans MPT | A3FJ49 | gene mxaJ; mxaFJGIR gene cluster encoding the large and small subunits, and protein mxaJ, organized in an operon and are transcribed as a single mRNA | - |
| Methylophaga aminisulfidivorans MPT | A3FJ51 | small subunit; mxaFJGIR gene cluster encoding the large and small subunits, and protein mxaJ, organized in an operon and are transcribed as a single mRNA | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native isozymes by anion exchange chromatography and gel filtration, type I 7.9fold, type II 14.7fold, the lysozyme and freeze-thawing cell disruption method significantly increases the amount of type II MDH in the soluble fraction compared with strong physical disruption methods such as sonication and French Press | Methylophaga aminisulfidivorans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.69 | - |
type I MDH, pH 7.0, 30°C, with cytochrome cL | Methylophaga aminisulfidivorans |
| 0.75 | - |
type II MDH, pH 7.0, 30°C, with cytochrome cL | Methylophaga aminisulfidivorans |
| 18.66 | - |
type I MDH, pH 9.0, 30°C, with phenazine ethosulfate and 2,6-dichlorophenolindophenol | Methylophaga aminisulfidivorans |
| 21.03 | - |
type II MDH, pH 9.0, 30°C, with phenazine ethosulfate and 2,6-dichlorophenolindophenol | Methylophaga aminisulfidivorans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methanol + 2 2,6-dichlorophenolindophenol | in the activity assay phenazine ethosulfate is used as primary electron acceptor, and 2,6-dichlorophenol indophenol as a terminal acceptor | Methylophaga aminisulfidivorans | formaldehyde + 2 reduced 2,6-dichlorophenolindophenol | - |
? | |
| methanol + 2 2,6-dichlorophenolindophenol | in the activity assay phenazine ethosulfate is used as primary electron acceptor, and 2,6-dichlorophenolindophenol as a terminal acceptor | Methylophaga aminisulfidivorans | formaldehyde + 2 reduced 2,6-dichlorophenolindophenol | - |
? | |
| methanol + 2 2,6-dichlorophenolindophenol | in the activity assay phenazine ethosulfate is used as primary electron acceptor, and 2,6-dichlorophenol indophenol as a terminal acceptor | Methylophaga aminisulfidivorans MPT | formaldehyde + 2 reduced 2,6-dichlorophenolindophenol | - |
? | |
| methanol + 2 2,6-dichlorophenolindophenol | in the activity assay phenazine ethosulfate is used as primary electron acceptor, and 2,6-dichlorophenolindophenol as a terminal acceptor | Methylophaga aminisulfidivorans MPT | formaldehyde + 2 reduced 2,6-dichlorophenolindophenol | - |
? | |
| methanol + 2 cytochrome cL | - |
Methylophaga aminisulfidivorans | formaldehyde + 2 reduced cytochrome cL | - |
? | |
| methanol + 2 cytochrome cL | native enzyme with pyrroloquinoline quinone as a prosthetic group and cytochrome cL as the primary electron acceptor | Methylophaga aminisulfidivorans | formaldehyde + 2 reduced cytochrome cL | - |
? | |
| methanol + 2 cytochrome cL | - |
Methylophaga aminisulfidivorans MPT | formaldehyde + 2 reduced cytochrome cL | - |
? | |
| methanol + 2 cytochrome cL | native enzyme with pyrroloquinoline quinone as a prosthetic group and cytochrome cL as the primary electron acceptor | Methylophaga aminisulfidivorans MPT | formaldehyde + 2 reduced cytochrome cL | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | the original conformation of the MDH Methylophaga aminisulfidivorans MPT is most likely the alpha2beta2-MxaJ complex | Methylophaga aminisulfidivorans |
| pentamer | 2 * 65980, subunit alpha, + 2 * 7580, subunit beta, + 1 * 27860, MxaJ protein, type II MDH consists of two identical dimers of alpha and beta subunits organized to form the alpha2beta2 tetramer, and contains an additional MxaJ protein | Methylophaga aminisulfidivorans |
| tetramer | 2 * 65980, subunit alpha, + 2 * 7580, subunit beta, type I MDH consists of two identical dimers of alpha and beta subunits organized to form the alpha2beta2 tetramer | Methylophaga aminisulfidivorans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| type I MDH | - |
Methylophaga aminisulfidivorans |
| type II MDH | - |
Methylophaga aminisulfidivorans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Methylophaga aminisulfidivorans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.11 | - |
methanol | type I MDH, pH 9.0, 30°C, with phenazine ethosulfate and 2,6-dichlorophenolindophenol | Methylophaga aminisulfidivorans | |
| 0.16 | - |
methanol | type II MDH, pH 9.0, 30°C, with phenazine ethosulfate and 2,6-dichlorophenolindophenol | Methylophaga aminisulfidivorans | |
| 2.96 | - |
methanol | type I MDH, pH 7.0, 30°C, with cytochrome cL | Methylophaga aminisulfidivorans | |
| 4.51 | - |
methanol | type II MDH, pH 7.0, 30°C, with cytochrome cL | Methylophaga aminisulfidivorans | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Methylophaga aminisulfidivorans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyrroloquinoline quinone | - |
Methylophaga aminisulfidivorans | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Methylophaga aminisulfidivorans | type I MDH | - |
5.4 |
| Methylophaga aminisulfidivorans | type II MDH | - |
5.8 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | type II MDH has higher specific activity than type I MDH. The original conformation of the MDH Methylophaga aminisulfidivorans MPT is most likely the alpha2beta2-MxaJ complex. The lysozyme and freeze-thawing cell disruption method significantly increases the amount of type II MDH in the soluble fraction compared with strong physical disruption methods such as sonication and French Press | Methylophaga aminisulfidivorans |
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