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show all sequences of 1.1.2.7

Purification, crystallization and preliminary X-ray crystallographic analysis of a methanol dehydrogenase from the marine bacterium Methylophaga aminisulfidivorans MP(T)

Choi, J.M.; Kim, H.G.; Kim, J.S.; Youn, H.S.; Eom, S.H.; Yu, S.L.; Kim, S.W.; Lee, S.H.; Acta Crystallogr. Sect. F 67, 513-516 (2011)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
purified native enzyme, hanging drop vapour diffusion method, 8 mg/ml protein in 25 mM Tris-HCl, pH 8.0, mixed with 0.1 M sodium cacodylate pH 6.5, 0.2 M magnesium acetate tetrahydrate and 10% v/v PEG 8000, 20°C, macrocrystals are soaked for 30 s in cryosolution consisting of crystallization solution with 20% v/v glycerol, X-ray diffraction structure determination and analysis at 1.7 A reolution, molecular replacement
Methylophaga aminisulfidivorans
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
periplasm
-
Methylophaga aminisulfidivorans
-
-
soluble
periplasmic region of the bacterial membrane
Methylophaga aminisulfidivorans
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
catalytic, active site-bound
Methylophaga aminisulfidivorans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
methanol + 2 cytochrome cL
Methylophaga aminisulfidivorans
-
formaldehyde + 2 reduced cytochrome cL
-
-
?
methanol + 2 cytochrome cL
Methylophaga aminisulfidivorans MPT
-
formaldehyde + 2 reduced cytochrome cL
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Methylophaga aminisulfidivorans
A3FJ51
small subunit; genes mxaF and mxaI, encoding the large and small subunit
-
Methylophaga aminisulfidivorans MPT
A3FJ51
small subunit; genes mxaF and mxaI, encoding the large and small subunit
-
Purification (Commentary)
Commentary
Organism
native active alpha2beta2 MDH complex by ultracentrifugation, anion echange chromatgraphy, and gel filtration
Methylophaga aminisulfidivorans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
methanol + 2 cytochrome cL
-
723854
Methylophaga aminisulfidivorans
formaldehyde + 2 reduced cytochrome cL
-
-
-
?
methanol + 2 cytochrome cL
-
723854
Methylophaga aminisulfidivorans MPT
formaldehyde + 2 reduced cytochrome cL
-
-
-
?
Subunits
Subunits
Commentary
Organism
heterotetramer
alpha2beta2, the MEDH heterotetramer is composed of two large subunits and two small subunits
Methylophaga aminisulfidivorans
More
the alpha-subunit is known to function as the active site for the oxidoreduction reaction, which includes the PQQ group as a redox cofactor and a calcium ion coordinated to vicinal charged and hydrophobic amino-acid residues
Methylophaga aminisulfidivorans
Cofactor
Cofactor
Commentary
Organism
Structure
pyrroloquinoline quinone
-
Methylophaga aminisulfidivorans
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Methylophaga aminisulfidivorans
-
-
5.4
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyrroloquinoline quinone
-
Methylophaga aminisulfidivorans
Crystallization (Commentary) (protein specific)
Crystallization
Organism
purified native enzyme, hanging drop vapour diffusion method, 8 mg/ml protein in 25 mM Tris-HCl, pH 8.0, mixed with 0.1 M sodium cacodylate pH 6.5, 0.2 M magnesium acetate tetrahydrate and 10% v/v PEG 8000, 20°C, macrocrystals are soaked for 30 s in cryosolution consisting of crystallization solution with 20% v/v glycerol, X-ray diffraction structure determination and analysis at 1.7 A reolution, molecular replacement
Methylophaga aminisulfidivorans
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
periplasm
-
Methylophaga aminisulfidivorans
-
-
soluble
periplasmic region of the bacterial membrane
Methylophaga aminisulfidivorans
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
catalytic, active site-bound
Methylophaga aminisulfidivorans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
methanol + 2 cytochrome cL
Methylophaga aminisulfidivorans
-
formaldehyde + 2 reduced cytochrome cL
-
-
?
methanol + 2 cytochrome cL
Methylophaga aminisulfidivorans MPT
-
formaldehyde + 2 reduced cytochrome cL
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
native active alpha2beta2 MDH complex by ultracentrifugation, anion echange chromatgraphy, and gel filtration
Methylophaga aminisulfidivorans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
methanol + 2 cytochrome cL
-
723854
Methylophaga aminisulfidivorans
formaldehyde + 2 reduced cytochrome cL
-
-
-
?
methanol + 2 cytochrome cL
-
723854
Methylophaga aminisulfidivorans MPT
formaldehyde + 2 reduced cytochrome cL
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
heterotetramer
alpha2beta2, the MEDH heterotetramer is composed of two large subunits and two small subunits
Methylophaga aminisulfidivorans
More
the alpha-subunit is known to function as the active site for the oxidoreduction reaction, which includes the PQQ group as a redox cofactor and a calcium ion coordinated to vicinal charged and hydrophobic amino-acid residues
Methylophaga aminisulfidivorans
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Methylophaga aminisulfidivorans
-
-
5.4
General Information
General Information
Commentary
Organism
metabolism
the electrons released from pyrroloquinoline quinone during the oxidation successively reduce cytochrome cL, cytochrome cH and finally the membrane oxidase cytochrome aa3. As a result of the oxidoreduction pathway, a proton electrochemical gradient is produced around the membrane, which in turn drives the generation of one ATP molecule per molecule of methanol
Methylophaga aminisulfidivorans
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the electrons released from pyrroloquinoline quinone during the oxidation successively reduce cytochrome cL, cytochrome cH and finally the membrane oxidase cytochrome aa3. As a result of the oxidoreduction pathway, a proton electrochemical gradient is produced around the membrane, which in turn drives the generation of one ATP molecule per molecule of methanol
Methylophaga aminisulfidivorans
Other publictions for EC 1.1.2.7
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724402
Gvozdev
Quinone-dependent alcohol dehy ...
Diplococcus sp., Methylophilus methylotrophus, Methylophilus methylotrophus W3A1, Methylorubrum extorquens, Paracoccus denitrificans, Paracoccus pantotrophus, Pseudomonas sp., Rhodoblastus acidophilus
Biochemistry
77
843-856
2012
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45
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7
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725300
Kim
Comparative analysis of two ty ...
Methylophaga aminisulfidivorans, Methylophaga aminisulfidivorans MPT
J. Basic Microbiol.
52
141-149
2012
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723854
Choi
Purification, crystallization ...
Methylophaga aminisulfidivorans, Methylophaga aminisulfidivorans MPT
Acta Crystallogr. Sect. F
67
513-516
2011
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724176
Li
The enzymatic reaction-induced ...
Methylophilus methylotrophus, Methylophilus methylotrophus W3A1
Biochem. Biophys. Res. Commun.
406
621-626
2011
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1
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712855
Idupulapati
Quantum chemical modeling of m ...
Methylorubrum extorquens
J. Phys. Chem. A
114
1887-1896
2010
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687417
Kalyuzhnaya
Characterization of a novel me ...
Methylibium petroleiphilum, Methyloversatilis universalis, Methyloversatilis universalis FAM5
J. Bacteriol.
190
3817-3823
2008
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673047
Leopoldini
The preferred reaction path fo ...
Methylophilus methylotrophus, Methylophilus methylotrophus W3A1
Chem. Eur. J.
13
2109-2117
2007
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39
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676902
Zhang
Mechanism of methanol oxidatio ...
Methylophilus methylotrophus
Proc. Natl. Acad. Sci. USA
104
745-749
2007
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667721
Nojiri
Crystal structures of cytochro ...
Hyphomicrobium denitrificans, Hyphomicrobium denitrificans A3151
Biochemistry
45
3481-3492
2006
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7
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1
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670785
Liu
A rapid method for the purific ...
Methylorubrum extorquens, Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1
Protein Expr. Purif.
46
316-320
2006
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688333
Williams
The 1.6A X-ray structure of th ...
Methylorubrum extorquens
J. Mol. Biol.
357
151-162
2006
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671068
Williams
The atomic resolution structur ...
Methylorubrum extorquens
Acta Crystallogr. Sect. D
D61
75-79
2005
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673048
Nojiri
-
Preparation and characterizati ...
Hyphomicrobium denitrificans, Hyphomicrobium denitrificans A3151
Chem. Lett.
34
1036-1037
2005
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684666
Anthony
The quinoprotein dehydrogenase ...
Methylophilus sp., Methylorubrum extorquens
Arch. Biochem. Biophys.
428
2-9
2004
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685313
Anthony
The structure and mechanism of ...
Methylophilus sp., Methylorubrum extorquens
Biochim. Biophys. Acta
1647
18-23
2003
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687993
Jongejan
Direct hydride transfer in the ...
Methylophilus methylotrophus, Methylorubrum extorquens
J. Comput. Chem.
22
1732-1749
2001
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689818
Zheng
Catalytic mechanism of quinopr ...
Methylophilus methylotrophus, Methylophilus methylotrophus W3A1
Proc. Natl. Acad. Sci. USA
98
432-434
2001
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687432
Grosse
Purification and properties of ...
Methylosinus sp., Methylosinus sp. WI 14
J. Basic Microbiol.
38
189-196
1998
2
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12
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689815
Zheng
Conformation of coenzyme pyrro ...
Methylophilus methylotrophus, Methylophilus methylotrophus W3A1
Proc. Natl. Acad. Sci. USA
94
11881-11886
1997
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