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EC Number
Metals/Ions
Commentary
Reference
2.7.1.32
Mg2+
-
662203
,
671362
2.7.1.32
Mn2+
-
641366
2.7.1.32
Mg2+
10 mM MgSO4 required for maximal activity
641357
2.7.1.32
Mg2+
11 mM used in assay conditions
760908
2.7.1.32
Na+
8.7-35 mM: slight activation, 70 mM: inactivation
641362
2.7.1.32
MgCl2
assay at
671383
2.7.1.32
Mg2+
ATP concentration 1.25-2.5 mM: reaction requires free Mg2+ rather than MgATP2-, ATP concentration exceeding that of Mg2+: strong inhibition
641345
2.7.1.32
Mn2+
cannot substitute for Mg2+
641364
2.7.1.32
more
Co2+, Cd2+: almost completely ineffective, Ca2+, Ni2+, Zn2+: totally ineffective
641359
,
641371
2.7.1.32
Mg2+
dependent on
739407
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